Megazyme/L-阿拉伯糖/D-半乳糖分析试剂盒/K-ARGA/115分析（手动）/1150分析（微孔板）/1150分析（自动分析仪
商品编号：
K-ARGA
品牌：
Megazyme INC
市场价：
¥4200.00
美元价：
2520.00
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
TheL-ArABInose/D-Galactosetestkitisa simple,reliableandaccurateUVmethodforthemeasurementandanalysisofL-arabinoseand/orD-galactoseinvariousmaterialsincludingfoods,feeds,beveragesandplantproducts.
Extendedcofactorsstability.Dissolvedcofactorsstablefor>1yearat4oC.
Suitableformanual,auto-analyserandmicroplateformats.
Hydrolysisofwheatflourarabinoxylan,acid-debranchedwheatflourarabinoxylanandarabino-xylo-oligosaccharidesbyβ-xylanase,α-L-arabinofuranosidaseandβ-xylosidase.
McCleary,B.V.,McKie,V.A.,Draga,A.,Rooney,E.,Mangan,D.&Larkin,J.(2015).CarbohydrateResearch,407,79-96.
LinktoArticle
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Arangeofα-L-arabinofuranosyl-(1-4)-β-D-xylo-oligosaccharides(AXOS)wereproducedbyhydrolysisofwheatflourarabinoxylan(WAX)andaciddebranchedarabinoxylan(ADWAX),inthepresenceandabsenceofanAXH-d3α-L-arabinofuranosidase,byseveralGH10andGH11β-xylanases.ThestructuresoftheoligosaccharideswerecharacterisedbyGC-MSandNMRandbyhydrolysisbyarangeofα-L-arabinofuranosidasesandβ-xylosidase.TheAXOSwerepurifiedandusedtocharacterisetheactionpatternsofthespecificα-L-arabinofuranosidases.Theseenzymes,incombinationwitheitherCellvibriomixtusorNeocallimastixpatriciarumβ-xylanase,wereusedtoproduceelevatedlevelsofspecificAXOSonhydrolysisofWAX,suchas32-α-L-Araf-(1-4)-β-D-xylobiose(A3X),23-α-L-Araf-(1-4)-β-D-xylotriose(A2XX),33-α-L-Araf-(1-4)-β-D-xylotriose(A3XX),22-α-L-Araf-(1-4)-β-D-xylotriose(XA2X),32-α-L-Araf(1-4)-β-D-xylotriose(XA3X),23-α-L-Araf-(1-4)-β-D-xylotetraose(XA2XX),33-α-L-Araf-(1-4)-β-D-xylotetraose(XA3XX),23,33-di-α-L-Araf-(1-4)-β-D-xylotriose(A2+3XX),23,33-di-α-L-Araf-(1-4)-β-D-xylotetraose(XA2+3XX),24,34-di-α-L-Araf-(1-4)-β-D-xylopentaose(XA2+3XXX)and33,34-di-α-L-Araf-(1-4)-β-D-xylopentaose(XA3A3XX),manyofwhichhavenotpreviouslybeenproducedinsufficientquantitiestoallowtheiruseassubstratesinfurtherenzymicstudies.ForA2,3XX,yieldsofapproximately16%ofthestartingmaterial(wheatarabinoxylan)havebeenachieved.Mixturesoftheα-L-arabinofuranosidases,withspecificactiononAXOS,havebeencombinedwithβ-xylosidaseandβ-xylanasetoobtainanoptimalmixtureforhydrolysisofarabinoxylantoL-arabinoseandD-xylose.
Novelbifunctionalα-L-arabinofuranosidase/xylobiohydrolase(ABF3)fromPenicilliumpurpurogenum.
Ravanal,M.C.,Callegari,E.&Eyzaguirre,J.(2010).AppliedandEnvironmentalMicroBIOLOGy,76(15),5247-5253.
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ThesoftrotfungusPenicilliumpurpurogenumgrowsonavarietyofnaturalsubstratesandsecretesvariousisoformsofxylanolyticenzymes,includingthreearabinofuranosidases.Thisworkdescribesthebiochemicalpropertiesaswellasthenucleotideandaminoacidsequencesofarabinofuranosidase3(ABF3).Thisenzymehasbeenpurifiedtohomogeneity.Itisaglycosylatedmonomerwithamolecularweightof50,700andcanbindcellulose.Theenzymeisactivewithp-nitrophenylα-L-arabinofuranosideandp-nitrophenylβ-D-xylopyranosidewithAKmof0.65mMand12mM,respectively.Theenzymeisactiveonxylooligosaccharides,yieldingproductsofshorterlength,includingxylose.However,itdoesnothydrolyzearabinooligosaccharides.Whenassayedwithpolymericsubstrates,littlearabinoseisliberatedfromarabinananddebranchedarabinan;however,ithydrolyzesarabinoseandreleasesxylooligosaccharidesfromarabinoxylan.SequencingbothABF3CDNAandgenomicDNArevealsthatthisgenedoesnotcontainintronsandthattheopenreADIngframeis1,380nucleotidesinlength.Thededucedmatureproteiniscomposedof433aminoacidsresiduesandhasacalculatedmolecularweightof47,305.ThededucedaminoacidsequencehasbeenvalidatedbymassspectrometryanalysisofpeptidesfrompurifiedABF3.Atotalof482bpofthepromoterweresequenced;putativebindingsitesfortranscriptionfactorssuchasCreA(four),XlnR(one),andAreA(three)andtwoCCAATboxeswerefound.Theenzymehastwodomains,onesimilartoproteinsofglycosylhydrolasefamily43attheamino-terminalendandafamily6carbohydratebindingmoduleatthecarboxylend.ABF3isthefirstdescribedmodularfamily43enzymefromafungalsource,havingbothα-L-arabinofuranosidaseandxylobiohydrolasefunctionalities.
TheinfluenceofAspergillusnigertranscriptionfactorsAraRandXlnRinthegeneexpressionduringgrowthinD-xylose,L-arabinoseandsteam-explodedsugarcanebagasse.
deSouza,W.R.,Maitan-Alfenas,G.P.,deGouvêa,P.F.,Brown,N.A.,Savoldi,M.,Battaglia,E.,GoldmanM.H.S.,deVries,R.P.&Goldman,G.H.(2013).FungalGeneticsandBiology,60,29-45.
LinktoArticle
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Theinterestintheconversionofplantbiomasstorenewablefuelssuchasbioethanolhasledtoanincreasedinvestigationintotheprocessesregulatingbiomasssaccharification.ThefilamentousfungusAspergillusnigerisanimportantmicroorganismcapableofproducingawidevarietyofplantbiomassdegradingenzymes.InA.nigerthetranscriptionalactivatorXlnRanditsclosehomolog,AraR,controlsthemain(hemi-)cellulolyticsystemresponsIBLeforplantpolysaccharidedegradation.Sugarcaneisusedworldwideasafeedstockforsugarandethanolproduction,whilethelignocellulosicresidualbagassecanbeusedindifferentindustrialapplications,includingethanolproduction.Theuseofpentosesugarsfromhemicellulosesrepresentsanopportunitytofurtherincreaseproductionefficiencies.Inthepresentstudy,wedescribeaglobalgeneexpressionanalysisofA.nigerXlnR-andAraR-deficientmutantstrains,grownonaD-xylose/L-arabinosemonosaccharidemixtureandsteam-explodedsugarcanebagasse.DifferentgenesetsofCAZyenzymesandsugartransporterswereshowntobeindividuallyorduallyregulatedbyXlnRandAraR,withXlnRappearingtobethemajorregulatoroncomplexpolysaccharides.Ourstudycontributestounderstandingofthecomplexregulatorymechanismsresponsibleforplantpolysaccharide-degradinggeneexpression,andopensnewpossibilitiesfortheengineeringoffungiabletoproducemoreefficientenzymaticcocktailstobeusedinbiofuelproduction.
HomologousexpressionandbiochemicalcharacterizationofthearylsulfatasefromKluyveromyceslactisanditsrelevanceinmilkprocessing.
Stressler,T.,Leisibach,D.,Lutz-Wahl,S.,Kuhn,A.&Fischer,L.(2016).AppliedMicrobiologyandBiotechnology,100(12),5401-5414.
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Theindustrialmanufacturingprocessoflactose-freemilkproductsdependsontheapplicationofcommercialβ-galactosidase(lactase)preparations.ThesepreparationsareoftenobtainedfromKluyveromyceslactis.ThereisagenepresentinthegenomeofK.lactiswhichshouldencodeforanenzymecalledarylsulfatase(EC3.1.6.1).Therefore,thisenzymecouldalsobepresentinβ-galactosidasepreparations.ThearylsulfataseissUSPectedofbeingresponsibleforanunpleasant“cowshed-like”off-flavorresultingfromthereleaseofp-cresolfrommilkendogenousalkylphenolsulfuricesters.Sofar,nogene/functionalityrelationshipisdescribed.Inaddition,nostudyisavailablewhichhasshownthatarylsulfatasefromK.lactisistrulyresponsiblefortheflavorgeneration.Inthisstudy,weclonedtheputativearylsulfatasegenefromK.lactisGG799intothecommerciallyavailablevectorpKLAC2.Thecloningstrategychosenresultedinahomologous,secretoryexpressionofthearylsulfatase.Weshowedthattheheretoforeputativearylsulfatasehasthedesiredactivitywiththesyntheticsubstratep-nitrophenylsulfateandwiththenaturalsubstratep-cresolsulfate.Theenzymewasbiochemicallycharacterizedandshowedanoptimumtemperatureof45-50 CandanoptimumpHof9-10.Additionally,thearylsulfatasewasactivatedbyCa2+ionsandwasinactivatedbyZn2+ions.Moreover,thearylsulfatasewasinhibitedbyp-cresolandsulfateions.Finally,theenzymewasaddedtoultra-heattreated(UHT)milkandasensorytriangletestverifiedthatthearylsulfatasefromK.lactiscancauseanunpleasant“cowshed-like”off-flavor.
UV-methodforthedeterminationofL-ArabinoseandD-Galactose
inhydrolysedplantproducts
Principle:
(galactosemutarotase)
(1)α-L-Arabinose/α-D-galactose↔β-L-arabinose/β-D-galactose
(β-galactosedehydrogenase)
(2)β-L-Arabinose+NAD+→L-arabinonicacid+NADH+H+
(β-galactosedehydrogenase)
(3)β-D-Galactose+NAD+→D-galactonicacid+NADH+H+
Kitsize: *115assays(manual)/1150(microplate)
/1150(auto-analyser)* Thenumberofmanualtestsperkitcanbedoubledifallvolumesarehalved.
ThiscanbereadilyaccommodatedusingtheMegaQuantTM Wave
Spectrophotometer(D-MQWAVE).Method: Spectrophotometricat340nm
Reactiontime: ~12min(L-arabinose)
~6min(D-galactose)
Detectionlimit: 0.58mg/L(L-arabinose)
0.69mg/L(D-galactose)
Applicationexamples:
Analysisofhydrolysatesofoligo-andpolysaccharides(e.g.arabinan,
arabinoxylan,galactan,arabinogalactan),milk,dairyproducts,foods
containingmilk(e.g.dieteticfoods,bakeryproducts,babyfood,
chocolate,sweetsandice-cream),foodadditives(e.g.sweeteners),
cosmetics,pharmaceuticalsandothermaterials(e.g.biological
cultures,samples,etc.)
Methodrecognition: Novelmethod
Advantages
- Veryrapidreactionduetoinclusionofgalactosemutarotase(patentedtechnology)
- Verycosteffective
- Allreagentsstablefor>2yearsafterpreparation
- Onlyenzymatickitavailable
- Mega-Calc™softwaretoolisavailablefromourwebsiteforhassle-freerawdataprocessing
- Standardincluded
- Suitableformanual,microplateandauto-analyserformats
品牌介绍
Megazyme品牌产品简介
来源：作者：人气：2149发表时间：2016-05-19 10:59:00【大 中 小】
Megazyme是一家全球性公司，专注于开发和提供用于饮料、谷物、乳制品、食品、饲料、发酵、生物燃料和葡萄酒产业用的分析试剂、酶和检测试剂盒。Megazyme的许多检测试剂盒产品已经为众多官方科学协会（包括AOAC, AACC , RACI, EBC和ICC等），经过严格的审核，批准认证为官方标准方法，确保以准确、可靠、定量和易于使用的测试方法，满足客户的质量诉求。
Megazyme的主要产品线包括：
◆ 检测试剂盒
◆ 酶
◆ 酶底物
◆ 碳水化合物
◆ 化学品/仪器
官网地址：http://www.megazyme.com
检测试剂盒特色产品：
货号
中文品名
用途
K-ACETAF
乙酸[AF法]检测试剂盒
酶法定量分析乙酸最广泛使用的方法
K-ACHDF
可吸收糖/膳食纤维检测试剂盒
酒精沉淀法测定膳食纤维
K-AMIAR
氨快速检测试剂盒
用于包括葡萄汁、葡萄酒以及其它食品饮料样品中氨含量的快速检测分析。
K-AMYL
直链淀粉/支链淀粉检测试剂盒
谷物淀粉和而粉中直链淀粉/支链淀粉比例和含量检测
K-ARAB
阿拉伯聚糖检测试剂盒
果汁浓缩液中阿拉伯聚糖的检测
K-ASNAM
L-天冬酰胺/L-谷氨酰胺和氨快速检测试剂盒
用于食品工业中丙烯酰胺前体、细胞培养基、以及上清液组分中、L-天冬酰胺，谷氨酰胺和氨的检测分析
K-ASPTM
阿斯巴甜检测试剂盒
专业用于测定饮料和食品中阿斯巴甜含量，操作简单
K-BETA3
β-淀粉酶检测试剂盒
适用于麦芽粉中β-淀粉酶的测定
K-BGLU
混合键β-葡聚糖检测试剂盒
测定谷物、荞麦粉、麦汁、啤酒及其它食品中混合键β-葡聚糖(1,3:1,4-β-D-葡聚糖)的含量
K-CERA
α-淀粉酶检测试剂盒
谷物和发酵液（真菌和细菌）中α-淀粉酶的分析测定
K-CITR
柠檬酸检测试剂盒
快速、可靠地检测食品、饮料和其它物料中柠檬酸（柠檬酸盐）含量
K-DLATE
乳酸快速检测试剂盒
快速、特异性检测饮料、肉类、奶制品和其它食品中L-乳酸和D-乳酸(乳酸盐）含量
K-EBHLG
酵母β-葡聚糖酶检测试剂盒
用于测量和分析酵母中1,3:1,6?-β-葡聚糖，也可以检测1,3-葡聚糖
K-ETSULPH
总亚硫酸检测试剂盒
测定葡萄酒、饮料、食品和其他物料中总亚硫酸含量（按二氧化硫计）的一种简单，高效，可靠的酶法检测方法
K-FRGLMQ
D-果糖/D-葡萄糖[MegaQuant法]检测试剂盒
适用于使用megaquant?色度计（505nm下）测定葡萄、葡萄汁和葡萄酒中D-果糖和D-葡萄糖的含量。
K-FRUC
果聚糖检测试剂盒
含有淀粉、蔗糖和其他糖类的植物提取物和食品中果聚糖的含量测定。
K-FRUGL
D-果糖/D-葡萄糖检测试剂盒
对植物和食品中果糖或葡萄糖含量的酶法紫外分光测定。
K-GALM
半乳甘露聚糖检测试剂盒
食品和植物产品中半乳甘露聚糖的含量检测
K-GLUC
D-葡萄糖[GOPOD]检测试剂盒
谷物提取物中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUHK
D-葡萄糖[HK]检测试剂盒
植物和食品中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUM
葡甘聚糖检测试剂盒
植物和食品中葡甘聚糖的含量测定。
K-INTDF
总膳食纤维检测试剂盒
总膳食纤维特定检测和分析
K-LACGAR
乳糖/D-半乳糖快速检测试剂盒
用于快速检测食品和植物产品中乳糖、D-半乳糖和L-阿拉伯糖
K-LACSU
乳糖/蔗糖/D-葡萄糖检测试剂盒
混合面粉和其它物料中蔗糖、乳糖和D-葡萄糖的测定
K-LACTUL
乳果糖检测试剂盒
特异性、快速和灵敏测量奶基样品中乳果糖含量
K-MANGL
D-甘露糖/D-果糖/D-葡萄糖检测试剂盒
适合测定植物产品和多糖酸性水解产物中D-甘露糖含量
K-MASUG
麦芽糖/蔗糖/D-葡萄糖检测试剂盒
在植物和食品中麦芽糖，蔗糖和葡萄糖的含量检测
K-PECID
胶质识别检测试剂盒
食品配料中果胶的鉴别
K-PHYT
植酸(总磷)检测试剂盒
食品和饲料样品植酸/总磷含量测量的简便方法。不需要通过阴离子交换色谱对植酸纯化，适合于大量样本分析
K-PYRUV
丙酮酸检测试剂盒
在啤酒、葡萄酒、果汁、食品和体液中丙酮酸分析
K-RAFGA
棉子糖/D-半乳糖检测试剂盒
快速测量植物材料和食品中棉子糖和半乳糖含量
K-RAFGL
棉子糖/蔗糖/D-半乳糖检测试剂盒
分析种子和种子粉中D-葡萄糖、蔗糖、棉子糖、水苏糖和毛蕊花糖含量。通过将棉子糖、水苏糖和毛蕊花糖酶解D-葡萄糖、D-果糖和半乳糖，从而测定葡萄糖含量来确定
K-SDAM
淀粉损伤检测试剂盒
谷物面粉中淀粉损伤的检测和分析
K-SUCGL
蔗糖/D-葡萄糖检测试剂盒
饮料、果汁、蜂蜜和食品中蔗糖和葡萄糖的分析
K-SUFRG
蔗糖/D-果糖/D-葡萄糖检测试剂盒
适用于植物和食品中蔗糖、D-葡萄糖和D-果糖的测定
K-TDFR
总膳食纤维检测试剂盒
总膳食纤维检测
K-TREH
海藻糖检测试剂盒
快速、可靠地检测食品、饮料和其它物料中海藻糖含量
K-URAMR
尿素/氨快速检测试剂盒
适用于水、饮料、乳制品和食品中尿素和氨的快速测定
K-URONIC
D-葡萄糖醛酸/D-半乳糖醛酸检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中六元糖醛酸含量（D-葡萄糖醛酸和D-半乳糖醛酸）
K-XYLOSE
D-木糖检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中D-木糖含量
K-YBGL
Beta葡聚糖[酵母和蘑菇]检测试剂盒
检测酵母和蘑菇制品中1,3:1,6-beta-葡聚糖和α-葡聚糖含量
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