Megazyme/D-果糖/D-葡萄糖分析试剂盒/K-FRUGL/110分析（手动）/1100分析（微孔板）/1100分析（自动分析仪）
商品编号：
K-FRUGL
品牌：
Megazyme INC
市场价：
¥3540.00
美元价：
2124.00
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
D-Fructose/D-Glucosetestkit,anenzymaticUV-methodforthemeasurementandanalysisofD-fructoseand/orD-glucoseinplantandfoodproducts.
ExtendedcofactorsstABIlity.Dissolvedcofactorsstablefor>1yearat 4oC.
Suitableformanual,auto-analyserandmicroplateformats.
Grapeandwineanalysis:Oenologiststoexploitadvancedtestkits.
Charnock,S.C.&McCleary,B.V.(2005).RevuedesEnology,117,1-5.
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Itiswithoutdoubtthattestingplaysapivotalrolethroughoutthewholeofthevinificationprocess.ToproducethebestpossIBLequalitywineandtominimiseprocessproblemssuchas“stuck”fermentationortroublesomeinfections,itisnowrecognisedthatifpossibletestingshouldbeginpriortoharvestingofthegrapesandcontinuethroughtobottling.TrADItionalmethodsofwineanalysisareoftenexpensive,timeconsuming,requireeitherelaborateequipmentorspecialistexpertiseandfrequentlylackaccuracy.However,enzymaticbio-analysisenablestheaccuratemeasurementofthevastmajorityofanalytesofinteresttothewinemaker,usingjustonepieceofapparatus,thespectrophotometer(seepreviousissueNo.116foradetailedtechnicalreview).Grapejuiceandwineareamenabletoenzymatictestingasbeingliquidstheyarehomogenous,easytomanipulate,andcangenerallybeanalysedwithoutanysamplepreparation.
Megazyme“advanced”winetestkitsgeneralcharacteristicsandvalidation.
Charnock,S.J.,McCleary,B.V.,Daverede,C.&Gallant,P.(2006).ReveuedesOenologues,120,1-5.
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ManyoftheenzymatictestkitsareofficialmethodsofprestigiousorganisationssuchastheAssociationofOfficialAnalyticalChemicals(AOAC)andtheAmericanAssociationofCerealChemists(AACC)inresponsetotheinterestfromoenologists.Megazymedecidedtouseitslonghistoryofenzymaticbio-analysistomakeasignificantcontributiontothewineindustry,bythedevelopmentofarangeofadvancedenzymatictestkits.Thistaskhasnowbeensuccessfullycompletedthroughthestrategicandcomprehensiveprocessofidentifyinglimitationsofexistingenzymaticbio-analysistestkitswheretheyoccurred,andthenusingadvancedtechniques,suchasmolecularBIOLOGy(photo1),torapidlyovercomethem.Noveltestkitshavealsobeendevelopedforanalytesofemerginginteresttotheoenologist,suchasyeastavailablenitrogen(YAN;seepages2-3ofissue117article),orwherepreviouslyenzymesweresimplyeithernotavailable,orweretooexpensivetoemploy,suchasforD-mannitolanalysis.
LactosefermentationbyKombucha–aprocesstoobtainnewmilk–basedbeverages.
Iličić,M.,Kanurić,K.,Milanović,S.,Lončar,E.,Djurić,M.&Malbaša,R.(2012).RomanianBiotechnologicalLetters,17(1),7013-7021.
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Thispaperfocusesonfermentationoflactosefromamodelsystem(blacktea)andfromtwotypesofmilk(0.9%w/wand2.2%w/woffat)byapplicationofKombucha.QuantitiesoftheappliedKombuchastarterwere10%v/vand15%v/v.Allfermentationswereperformedat42°C.TheprocesstoachieveadesirablepH=4.5wasslowerinthemodelsystem(16h)thaninmilks(9-10h).Regardingstarterquantity,10%v/vprovedtheoptimal.Regardingtypesofmilk,higherfatcontentguaranteesshorterfermentationandhigheryieldofmetabolites.Utilizationoflactosewasfoundatalevelof≈20%and≈30%inmilkswith0.9%w/wand2.2%w/woffat,respectively.Thiswascorrelatedwithanappearanceofintermediatesand/orproducts.Glucoseunderwentfurthertransformationsalmostentirely,whilegalactoseshowedmuchlowerreactivity.Seventotwelvetimeshighercontentsoflacticacidwerefoundcomparedtoaceticacid.Milk-basedbeveragefromthereducedfatsample,inoculatedwith10%v/vofKombuchastarter,hasthebestphysicalcharacteristics(syneresisandwaterholdingcapacity).Italsodevelopedagoodtexture(especiallycohesivenessandindexofviscosity).Milklactosefermentationwasaprocessthatcouldhavebeenusedforobtainingnewmilk-basedproducts.
Sourdough-leavenedbreadimprovespostprandialglucoseandinsulinplasmalevelsinsubjectswithimpairedglucosetolerance.
Maioli,M.,Pes,G.M.,Sanna,M.,Cherchi,S.,Dettori,M.,Manca,E.&Farris,G.A.(2008).ActaDiabetologica,45(2),91-96.
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Sourdoughbreadhasbeenreportedtoimproveglucosemetabolisminhealthysubjects.Inthisstudypostprandialglycaemicandinsulinaemicresponseswereevaluatedinsubjectswithimpairedglucosetolerance(IGT)whohadamealcontainingsourdoughbreadleavenedwithlactobacilli,incomparisontoareferencemealcontainingbreadleavenedwithbakeryeast.SixteenIGTsubjects(agerange52–75,averageBMI29.9±4.2kg/m2)wererandomlygivenamealcontainingsourdoughbread(A)andamealcontainingthereferencebread(B)intwoseparateoccasionsatthebeginningofthestudyandafter7days.Sourdoughbreadwasleavenedfor8husingastartercontainingautochthonousSaccharomycescerevisiaeandseveralbacilliabletoproduceasignificantamountofD-andL-lacticacid,whereasthereferencebreadwasleavenedfor2hwithcommercialbakeryeastcontainingSaccharomycescerevisiae.Plasmaglucoseandinsulinlevelsweremeasuredattime0,30,60,120,and180min.InIGTsubjectssourdoughbreadinducedasignificantlylowerplasmaglucoseresponseat30minutes(p=0.048)andasmallerincrementalareaundercurve(AUC)Δ0–30andΔ0–60min(p=0.020and0.018respectively)incomparisontothebreadleavenedwithbakeryeast.Plasmainsulinresponsetothistypeofbreadshowedlowervaluesat30min(p=0.045)andasmallerAUCΔ0–30min(p=0.018).ThisstudyshowsthatinsubjectswithIGTglycaemicandinsulinaemicresponsesaftertheconsumptionofsourdoughbreadarelowerthanafterthebreadleavenedwithbakeryeast.Thiseffectislikelyduetothelacticacidproducedduringdoughleaveningaswellasthereducedavailabilityofsimplecarbohydrates.Thus,sourdoughbreadmaypotentiallybeofbenefitinsubjectswithimpairedglucosemetabolism.
Endocrineandmetaboliceffectsofconsumingfructose-andglucose-sweetenedbeverageswithmealsinobesemenandwomen:Influenceofinsulinresistanceonplasmatriglycerideresponses.
Teff,K.L.,Grudziak,J.,Townsend,R.R.,Dunn,T.N.,Grant,R.W.,Adams,S.H.,Keim,N.L.,Cummings,B.P.,Stanhope,K.L.&Havel,P.J.(2009).JournalofClinicalEndocrinology&Metabolism,94(5),1562-1569.
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Context:Comparedwithglucose-sweetenedbeverages,consumptionoffructose-sweetenedbeverageswithmealselevatespostprandialplasmatriglyceridesandlowers24-hinsulinandleptinprofilesinnormal-weightwomen.Theeffectsoffructose,comparedwithglucose,ingestiononmetabolicprofilesinobesesubjectshasnotbeenstudied.Objective:Theobjectiveofthestudywastocomparetheeffectsoffructose-andglucose-sweetenedbeveragesconsumedwithmealsonhormonesandmetabolicsubstratesinobesesubjects.DesignandSetting:Thestudyhadawithin-subjectdesignconductedintheclinicalandtranslationalresearchcenter.Participants:Participantsincluded17obesemen(n=9)andwomen(n=8),withabodymassindexgreaterthan30kg/m2.Interventions:Subjectswerestudiedundertwoconditionsinvolvingingestionofmixednutrientmealswitheitherglucose-sweetenedbeveragesorfructose-sweetenedbeverages.Thebeveragesprovided30%oftotalkilocalories.Bloodsampleswerecollectedover24h.MainOutcomeMeasures:Areaunderthecurve(24hAUC)forglucose,lactate,insulin,leptin,ghrelin,uricacid,triglycerides(TGs),andfreefattyacidswasmeasured.Results:Comparedwithglucose-sweetenedbeverages,fructoseconsumptionwasassociatedwithlowerAUCsforinsulin(1052.6±135.1vs.549.2±79.7μU/mlper23h,P<0.001)=""and=""leptin=""(151.9=""±=""22.7=""vs.=""107.0=""±=""15.0=""ng/ml=""per=""24=""h,="">P<0.03)=""and=""increased=""auc=""for=""tg=""(242.3=""±=""96.8=""vs.=""704.3=""±=""124.4=""mg/dl=""per=""24=""h,="">P<0.0001).=""insulin-resistant=""subjects=""exhibited=""larger=""24-h=""tg=""profiles="">P<0.03).="">Conclusions:Inobesesubjects,consumptionoffructose-sweetenedbeverageswithmealswasassociatedwithlessinsulinsecretion,blunteddiurnalleptinprofiles,andincreasedpostprandialTGconcentrationscomparedwithglucoseconsumption.IncreasesofTGswereaugmentedinobesesubjectswithinsulinresistance,suggestingthatfructoseconsumptionmayexacerbateanalreadyadversemetabolicprofilepresentinmanyobesesubjects.Comparedtoglucose-sweetenedbeverages,consumptionoffructose-sweetenedbeveragesincreasespostprandialtriglyceridesinobesesubjects,potentiallyexacerbatingtheknownadversemetabolicprofileassociatedwithobesity.
Metabolicandendocrineprofilesinresponsetosystemicinfusionoffructoseandglucoseinrhesusmacaques.
Adams,S.H.,Stanhope,K.L.,Grant,R.W.,Cummings,B.P.&Havel,P.J.(2008).Endocrinology,149(6),3002-3008.
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Diurnalpatternsofcirculatingleptinconcentrationsareattenuatedafterconsumptionoffructose-sweetenedbeveragescomparedwithglucose-sweetenedbeverages,likelyaresultoflimitedpostprandialglucoseandinsulinexcursionsafterfructose.Differencesinpostprandialexposureofadiposetissuetoperipheralcirculatingfructoseandglucoseorinadipocytemetabolismofthetwosugarsmayalsobeinvolved.Thus,wecomparedplasmaleptinconcentrationsafter6-hivinfusionsofsaline,glucose,orfructose(15mg/kg•min)inovernight-fastedadultrhesusmonkeys(n=9).Despiteincreasesofplasmafructosefromundetectablelevelstoabout2mmduringfructoseinfusion,plasmaleptinconcentrationsdidnotincrease,andthechangeofinsulinwasonlyabout10%ofthatseenduringglucoseinfusion.Duringglucoseinfusion,plasmaleptinwassignificantlyincreasedabovebaselineconcentrationsby240minandincreasedsteadilyuntilthefinal480-mintimepoint(changeinleptin=+2.5±0.9ng/ml,P<0.001="">vs.saline;percentchangeinleptin=+55±16%;P<0.005="">vs.saline).Substantialanaerobicmetabolismoffructosewassuggestedbyalargeincreaseofsteady-stateplasmalactate(changeinlactate=1.64±0.15mmfrombaseline),whichwassignificantlygreaterthanthatduringglucose(+0.53±0.14mm)orsaline(−0.51±0.14mm)infusions(P<0.001).=""therefore,=""increased=""adipose=""exposure=""to=""fructose=""and=""an=""active=""whole-body=""anaerobic=""fructose=""metabolism=""are=""not=""sufficient=""to=""increase=""circulating=""leptin=""levels=""in=""rhesus=""monkeys.=""thus,=""additional=""factors="">i.e.limitedpost-fructoseinsulinexcursionsand/orhexose-specificdifferencesinadipocytemetabolism)arelikelytounderliedisparateeffectsoffructoseandglucosetoincreasecirculatingleptinconcentrations.
β-FructofuranosidaseandsucrosephosphorylaseofrumenbacteriumPseudobutyrivibrioruminisstrain3.
Kasperowicz,A.,Stan-Glasek,K.,Guczynska,W.,Pristas,P.,Javorsky,P.,Vandzurova,A.&Michalowski,T.(2012).WorldJournalofMicrobiologyandBiotechnology,28(3),1271-1279.
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ThesubjectofthisstudywasthefructanandsucrosedegradingenzymesofbacteriumPseudobutyrivibrioruminisstrain3.Itwasstatedthatcellextractfrombacteriagrowingoninulincontainedβ-fructofuranosidase(EC3.2.1.80and/orEC3.2.1.26)andsucrosephosphorylase(EC2.4.1.7),whilethebacteriamaintainedonsucroseshowedonlyphosphorylase.Partiallypurifiedβ-fructofuranosidasedigestedinulooligosaccharidesandsucrosetofructoseorfructoseandglucose,respectively,butwasunabletodegradethelongchainpolymersofcommercialinulinandTimothygrassfructan.DigestionrateofinulooligosaccharidesfitMichaelis–MentenkineticswithVmax5.64μM/mg/minandKm1.274%,respectively,whilethatofsucrosewaslinear.Partiallypurifiedsucrosephosphorylasedigestedonlysucrose.Thedigestionproductswerefructose,glucose-1Pandfreeglucose.ThereactionwasinagreementwithMichaelis–Mentenkinetics.TheVmaxwere0.599and0.584μM/mg/min,whileKmwere0.190and0.202%forfructosereleaseandglucose-1Pformation,respectively,whenbacteriagrewoninulin.TheVmaxwere,however,1.37and1.023μM/mg/min,whileKmwere0.264and0.156%,ifbacteriaweregrownonsucrose.Thefreeglucosewashardlydetectablefortheenzymeoriginatedfrominulingrownbacteria,butglucoselevelsrangedfrom0.05to0.25μM/mg/min,whencellextractfrombacteriagrownonsucrosewasused.Releaseoffreeglucosewasobservedwhennoinorganicphosphatewaspresentinreactionmixture.
ComparativeeffectsoffructoseandglucoseonlipogenicgeneexpressionandintermediarymetabolisminHepG2livercells.
Hirahatake,K.M.,Meissen,J.K.,Fiehn,O.&Adams,S.H.(2011).PloSone,6(11),e26583.
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Consumptionoflargeamountsoffructoseorsucroseincreaseslipogenesisandcirculatingtriglyceridesinhumans.Althoughtheunderlyingmolecularmechanismsresponsibleforthiseffectarenotcompletelyunderstood,itispossiblethatasreportedforrodents,highfructoseexposureincreasesexpressionofthelipogenicenzymesfattyacidsynthase(FAS)andacetyl-CoAcarboxylase(ACC-1)inhumanliver.Sinceactivationofthehexosaminebiosynthesispathway(HBP)isassociatedwithincreasesintheexpressionofFASandACC-1,itraisesthepossibilitythatHBP-relatedmetaboliteswouldcontributetoanyincreaseinhepaticexpressionoftheseenzymesfollowingfructoseexposure.Thus,wecomparedlipogenicgeneexpressioninhuman-derivedHepG2cellsafterincubationinculturemediumcontainingglucosealoneorglucoseplus5mMfructose,usingtheHBPprecursor10mMglucosamine(GlcN)asapositivecontrol.Cellularmetaboliteprofilingwasconductedtoanalyzedifferencesbetweenglucoseandfructosemetabolism.DespiteevidencefortheactiveuptakeandmetabolismoffructosebyHepG2cells,expressionofFASorACC-1didnotincreaseinthesecellscomparedwiththoseincubatedwithglucosealone.LevelsofUDP-N-acetylglucosamine(UDP-GlcNAc),theend-productoftheHBP,didnotdiffersignificantlybetweentheglucoseandfructoseconditions.Exposureto10mMGlcNfor10minutesto24hoursresultedin8-foldelevatedlevelsofintracellularUDP-GlcNAc(P<0.001), as="" well="" as="" a="" 74–126%="" increase="" in="" fas="">P<0.05) and="" 49–95%="" increase="" in="" acc-1="">P<0.01) expression="" above="" controls.="" it="" is="" concluded="" that="" in="" hepg2="" liver="" cells="" cultured="" under="" standard="" conditions,="" sustained="" exposure="" to="" fructose="" does="" not="" result="" in="" an="" activation="" of="" the="" hbp="" or="" increased="" lipogenic="" gene="" expression.="" should="" this="" scenario="" manifest="" in="" human="" liver="">invivo,itwouldsuggestthathighfructoseconsumptionpromotestriglyceridesynthesisprimarilythroughitsactiontoprovidelipidprecursorcarbonandnotbyactivatinglipogenicgeneexpression.
Measurementofglucoseandfructoseinclinicalsamplesusinggaschromatography/massspectrometry.
Wahjudi,P.N.,Patterson,M.E.,Lim,S.,Yee,J.K.,Mao,C.S.&Lee,W.N.P.(2010).ClinicalBiochemistry,43(1-2),198-207.
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Objective:Theimpactofincreasedfructoseconsumptiononcarbohydratemetabolismisatopicofcurrentinterest,butdeterminationofserumlevelhasbeenhinderedduetolowconcentrationandinterferencefromserumglucose.Wearereportingamethodforthequantificationofglucoseandfructoseinclinicalsamplesusinggaschromatography/massspectrometry(GC/MS).TheaccuracyandprecisionofGC/MSandanenzymaticassaywerecompared.Designandmethods:Massspectrometryfragmentationpatternsofmethyloximeperacetatederivatizedaldoseandketoseweredetermined.Uniquefragmentsforglucoseandfructosewereusedforquantitativeanalysisusingisotopelabeledrecoverystandards.Results:Methyloximeperacetatederivativesofglucoseandfructoseshowedcharacteristiclossofacetate(M-60)orketene(M-42)underchemicalionization(CI).Underelectronimpact(EI)ionization,auniqueC1–C2fragmentofglucosewasformed,whileaC1–C3fragmentwasformedfromketo-hexoses.Theseuniquefragmentswereusedinthequantitativeassayofglucoseandfructoseinclinicalsamples.Inclinicalsamples,theGC/MSassayhasalowerlimitofdetectionthanthatoftheenzymaticassay.Inplasmasamplesfrompatientsevaluatedfordiabetestheaverageserumglucoseandfructosewere6.19±2.72mMand46±25.22μM.Fructoseconcentrationsinmanyofthesesampleswerebelowthelimitofdetectionoftheenzymaticmethod.Conclusion:DerivatizationofaldoseandketosemonosaccharidestotheirrespectiveO-methyloximeacetatesforGC/MSanalysisisafacilemethodfordeterminationofserum/plasmaglucoseandfructosesamples.
PhysiologicalstudiesofLeuconostocmesenteroidesstrainNRRLB-1149duringcultivationonglucoseandfructosemedia.
Bivolarski,V.,Vasileva,T.,Shukla,R.,Goyal,A.&Iliev,I.(2012).JournalofBioScience&Biotechnology,1(3),235-240.
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Glycosyltransferasesareextracellularandcell-associatedsucraseenzymesproducedmainlybylacticacidbacteriaLeuconostocmesenteroides,oralStreptococcusspeciesandalsoLactobacillusspecies.Accordingtothesynthesizedpolymer(glucanorfructan)inthepresenceofsucrose,theseenzymesaredividedintotwogroups:glucosyltransferases(GTFs)andfructosyltransferases(FTFs).OnlyStreptococcus,LactobacillusandLeuconostocstrainsareknownasproducersofbothGTFsandFTFs.TheenzymesfromLactobacillusandLeuconostocspp.areimplicatedinthesynthesisofpolymersandoligosaccharides(OS)importantforhumanhealthbecauseoftheirprebioticpropertiesandimmunomodulatingactivity.Inthepresentwork,westudiedtheproductionofextracellularandcell-associatedglycosyltransferasesbyLeuconostocmesenteroidesstrainNRRLB-1149duringitsgrowthonmediacontainingglucoseorfructoseasamaincarbonsource.Theenzymeactivities,pHandbiomassformationweremeasuredandcomparedduringthecultivation.Wehaveshownthatglucoseandfructosehavenotanequalroleforenzymeproduction.Thehighestextracellularactivitywasdetectedatthe4thhourduringthecultivationofthestraininmediumwithfructose-5.45U/mg.Whenthestrainwascultivatedinmediumwithglucose,themaximumofextracellularenzymeactivitywasdetectedatthe5thhourofthecultivationbutthemeasuredactivitywasabout9timeslowercomparedtothese,obtainedaftercultivationinfructosemedium.Thestudiedstrainproducedmainlyextracellularglycosyltransferasesinglucoseorfructosemedium,whichwere92.4%and97.1%ofthetotalenzymeactivity,respectively.Inordertocharacterizetheproducedenzymes,cell-associatedandextracellularenzymesweredeterminedusingSDS-PAGEandinsituPeriodicAcidSchiff"sstainingafterincubationwith10%sucrose.Whentheinvestigatedstrainwasgrowninmediawithsucrose,glucoseorfructose,severaltypesofglycosyltransferasesweredetected-dextransucrasewithmolecularweight180kDaandtwofructosyltransferases,correspondingto120kDaand86kDamolecularweights.
Aldosereductaseisimplicatedinhighglucose‐inducedoxidativestressinmouseembryonicneuralstemcells.
Fu,J.,Tay,S.S.W.,Ling,E.A.&Dheen,S.T.(2007).JournalofNeuRochemistry,103(4),1654-1665.
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Oxidativestresscausedbyhyperglycemiaisoneofthekeyfactorsresponsibleformaternaldiabetes-inducedcongenitalmalformations,includingneuraltubedefectsinembryos.However,mechanismsbywhichmaternaldiabetesinducesoxidativestressduringneurulationarenotclear.Thepresentstudywasaimedtoinvestigatewhetherhighglucoseinducesoxidativestressinneuralstemcells(NSCs),whichcomposetheneuraltubeduringdevelopment.WealsoinvestigatedthemechanismbywhichhighglucosedisturbsthegrowthandsurvivalofNSCsinvitro.NSCswereexposedtophysiologicalD-glucoseconcentration(PG,5mmol/L),PGwithL-glucose(25mmol/L),orhighD-glucoseconcentration(HG,30or45mmol/l).HGinducedreactiveoxygenspeciesproductionandmRNAexpressionofaldosereductase(AR),whichcatalyzestheglucosereductionthroughpolyolpathway,inNSCs.Expressionofglucosetransporter1(Glut1)mRNAandproteinwhichregulatesglucoseuptakeinNSCswasincreasedatearlystage(24h)andbecamedown-regulatedatlatestage(72h)ofexposuretoHG.InhibitionofARbyfidarestat,anARinhibitor,decreasedtheoxidativestress,restoredthecellviabilityandproliferation,andreducedapoptoticcelldeathinNSCsexposedtoHG.Moreover,inhibitionofARattenuatedthedown-regulationofGlut1expressioninNSCsexposedtoHGfor72h.TheseresultssuggestthattheactivationofpolyolpathwayplaysaroleintheinductionofoxidativestresswhichaltersGlut1expressionandcellcycleinNSCsexposedtoHG,therebyresultinginabnormalpatterningoftheneuraltubeinembryosofdiabeticpregnancy.
Immobilisedyeastgrapemustdeacidificationinarecyclefixedbedreactor.
Portugal,I.,Ribeiro,S.C.,Xavier,A.M.R.B.,Centeno,F.&Strehaiano,P.(2011).InternationalJournalofFoodScience&Technology,46(2),284-289.
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Maloalcoholicfermentation(MAF)ofgrapemustbySchizosaccharomycespombeimmobilisedincalcium-alginatedouble-layerbeads(ProMalic®)wasstudiedinErlenmeyerflasksandinatotalrecyclefixed-bedreactoroperatinginbatchmode.Thereactionispseudo-firstorderwithrespecttoL-malicacidandundersimilarconditionsdeacidificationisfasterintherecyclereactor.ThiswasattributedtomasstransferlimitationswhichwereconfirmedintherecyclereactorbystudyingtheinfluenceofyeastloadontherateofMAF.Masstransferlimitationsarealsoresponsiblefortheloweractivationenergyoffermentationwiththeimmobilisedyeast(67±9kJmol-1)incomparisonwiththefreecells(126±19kJmol-1).AlcoholicfermentationandMAFwereperformedsimultaneously,bothintherecyclereactorandintheindustrialtrials,confirmingtheefficacyofimmobilisedS.pombetoreducegrapemustaciditywithoutinterferingwiththemainfermentation.Altogether,thepresentresultsareusefulforthescale-upofarecyclereactortoprocesslargevolumesofgrapemust.
UV-methodforthedeterminationofD-FructoseandD-Glucose
infoodstuffs,beveragesandothermaterials
Principle:
(hexokinase)
(1)D-Glucose+ATP→G-6-P+ADP
(hexokinase)
(2)D-Fructose+ATP→F-6-P+ADP
(glucose-6-phosphatedehydrogenase)
(3)G-6-P+NADP+→gluconate-6-phosphate+NADPH+H+
(phosphoglucoseisomerase)
(4)F-6-P ↔ G-6-P
Kitsize: * 110assays(manual)/1100(microplate)
/1100(auto-analyser)* Thenumberofmanualtestsperkitcanbedoubledifallvolumesarehalved.
ThiscanbereadilyaccommodatedusingtheMegaQuantTM Wave
Spectrophotometer(D-MQWAVE).Method: Spectrophotometricat340nm
Reactiontime: ~13min
Detectionlimit: 0.66mg/L
Applicationexamples:
Wine,beer,fruitjuices,softdrinks,milk,jam,honey,dieteticfoods,
bread,bakeryproducts,candies,desserts,confectionery,ice-cream,
fruitandvegetables,condiments,tobacco,cosmetics,pharmaceuticals,
paperandothermaterials(e.g.biologicalcultures,samples,etc.)
Methodrecognition:
MethodsbasedonthisprinciplehavebeenacceptedbyAOAC,EN,
NEN,NF,DIN,GOST,OIV,IFU,AIJN,MEBAKandIOCCC
Advantages
- PVPincorporatedtopreventtannininhibition
- ValidatedbytheUniversityofWine,SuzelaRousse,France
- Verycompetitiveprice(costpertest)
- Allreagentsstablefor>2yearsafterpreparation(manualanalysisapplications)
- Rapidreactionateither25or37°C
- Mega-Calc™softwaretoolisavailablefromourwebsiteforhassle-freerawdataprocessing
- Standardincluded
- Extendedcofactorsstability
- Suitableformanual,microplateandauto-analyserformats
品牌介绍
Megazyme品牌产品简介
来源：作者：人气：2149发表时间：2016-05-19 10:59:00【大 中 小】
Megazyme是一家全球性公司，专注于开发和提供用于饮料、谷物、乳制品、食品、饲料、发酵、生物燃料和葡萄酒产业用的分析试剂、酶和检测试剂盒。Megazyme的许多检测试剂盒产品已经为众多官方科学协会（包括AOAC, AACC , RACI, EBC和ICC等），经过严格的审核，批准认证为官方标准方法，确保以准确、可靠、定量和易于使用的测试方法，满足客户的质量诉求。
Megazyme的主要产品线包括：
◆ 检测试剂盒
◆ 酶
◆ 酶底物
◆ 碳水化合物
◆ 化学品/仪器
官网地址：http://www.megazyme.com
检测试剂盒特色产品：
货号
中文品名
用途
K-ACETAF
乙酸[AF法]检测试剂盒
酶法定量分析乙酸最广泛使用的方法
K-ACHDF
可吸收糖/膳食纤维检测试剂盒
酒精沉淀法测定膳食纤维
K-AMIAR
氨快速检测试剂盒
用于包括葡萄汁、葡萄酒以及其它食品饮料样品中氨含量的快速检测分析。
K-AMYL
直链淀粉/支链淀粉检测试剂盒
谷物淀粉和而粉中直链淀粉/支链淀粉比例和含量检测
K-ARAB
阿拉伯聚糖检测试剂盒
果汁浓缩液中阿拉伯聚糖的检测
K-ASNAM
L-天冬酰胺/L-谷氨酰胺和氨快速检测试剂盒
用于食品工业中丙烯酰胺前体、细胞培养基、以及上清液组分中、L-天冬酰胺，谷氨酰胺和氨的检测分析
K-ASPTM
阿斯巴甜检测试剂盒
专业用于测定饮料和食品中阿斯巴甜含量，操作简单
K-BETA3
β-淀粉酶检测试剂盒
适用于麦芽粉中β-淀粉酶的测定
K-BGLU
混合键β-葡聚糖检测试剂盒
测定谷物、荞麦粉、麦汁、啤酒及其它食品中混合键β-葡聚糖(1,3:1,4-β-D-葡聚糖)的含量
K-CERA
α-淀粉酶检测试剂盒
谷物和发酵液（真菌和细菌）中α-淀粉酶的分析测定
K-CITR
柠檬酸检测试剂盒
快速、可靠地检测食品、饮料和其它物料中柠檬酸（柠檬酸盐）含量
K-DLATE
乳酸快速检测试剂盒
快速、特异性检测饮料、肉类、奶制品和其它食品中L-乳酸和D-乳酸(乳酸盐）含量
K-EBHLG
酵母β-葡聚糖酶检测试剂盒
用于测量和分析酵母中1,3:1,6?-β-葡聚糖，也可以检测1,3-葡聚糖
K-ETSULPH
总亚硫酸检测试剂盒
测定葡萄酒、饮料、食品和其他物料中总亚硫酸含量（按二氧化硫计）的一种简单，高效，可靠的酶法检测方法
K-FRGLMQ
D-果糖/D-葡萄糖[MegaQuant法]检测试剂盒
适用于使用megaquant?色度计（505nm下）测定葡萄、葡萄汁和葡萄酒中D-果糖和D-葡萄糖的含量。
K-FRUC
果聚糖检测试剂盒
含有淀粉、蔗糖和其他糖类的植物提取物和食品中果聚糖的含量测定。
K-FRUGL
D-果糖/D-葡萄糖检测试剂盒
对植物和食品中果糖或葡萄糖含量的酶法紫外分光测定。
K-GALM
半乳甘露聚糖检测试剂盒
食品和植物产品中半乳甘露聚糖的含量检测
K-GLUC
D-葡萄糖[GOPOD]检测试剂盒
谷物提取物中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUHK
D-葡萄糖[HK]检测试剂盒
植物和食品中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUM
葡甘聚糖检测试剂盒
植物和食品中葡甘聚糖的含量测定。
K-INTDF
总膳食纤维检测试剂盒
总膳食纤维特定检测和分析
K-LACGAR
乳糖/D-半乳糖快速检测试剂盒
用于快速检测食品和植物产品中乳糖、D-半乳糖和L-阿拉伯糖
K-LACSU
乳糖/蔗糖/D-葡萄糖检测试剂盒
混合面粉和其它物料中蔗糖、乳糖和D-葡萄糖的测定
K-LACTUL
乳果糖检测试剂盒
特异性、快速和灵敏测量奶基样品中乳果糖含量
K-MANGL
D-甘露糖/D-果糖/D-葡萄糖检测试剂盒
适合测定植物产品和多糖酸性水解产物中D-甘露糖含量
K-MASUG
麦芽糖/蔗糖/D-葡萄糖检测试剂盒
在植物和食品中麦芽糖，蔗糖和葡萄糖的含量检测
K-PECID
胶质识别检测试剂盒
食品配料中果胶的鉴别
K-PHYT
植酸(总磷)检测试剂盒
食品和饲料样品植酸/总磷含量测量的简便方法。不需要通过阴离子交换色谱对植酸纯化，适合于大量样本分析
K-PYRUV
丙酮酸检测试剂盒
在啤酒、葡萄酒、果汁、食品和体液中丙酮酸分析
K-RAFGA
棉子糖/D-半乳糖检测试剂盒
快速测量植物材料和食品中棉子糖和半乳糖含量
K-RAFGL
棉子糖/蔗糖/D-半乳糖检测试剂盒
分析种子和种子粉中D-葡萄糖、蔗糖、棉子糖、水苏糖和毛蕊花糖含量。通过将棉子糖、水苏糖和毛蕊花糖酶解D-葡萄糖、D-果糖和半乳糖，从而测定葡萄糖含量来确定
K-SDAM
淀粉损伤检测试剂盒
谷物面粉中淀粉损伤的检测和分析
K-SUCGL
蔗糖/D-葡萄糖检测试剂盒
饮料、果汁、蜂蜜和食品中蔗糖和葡萄糖的分析
K-SUFRG
蔗糖/D-果糖/D-葡萄糖检测试剂盒
适用于植物和食品中蔗糖、D-葡萄糖和D-果糖的测定
K-TDFR
总膳食纤维检测试剂盒
总膳食纤维检测
K-TREH
海藻糖检测试剂盒
快速、可靠地检测食品、饮料和其它物料中海藻糖含量
K-URAMR
尿素/氨快速检测试剂盒
适用于水、饮料、乳制品和食品中尿素和氨的快速测定
K-URONIC
D-葡萄糖醛酸/D-半乳糖醛酸检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中六元糖醛酸含量（D-葡萄糖醛酸和D-半乳糖醛酸）
K-XYLOSE
D-木糖检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中D-木糖含量
K-YBGL
Beta葡聚糖[酵母和蘑菇]检测试剂盒
检测酵母和蘑菇制品中1,3:1,6-beta-葡聚糖和α-葡聚糖含量
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