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Megazyme/The D-Fructose and D-Glucose Assay Kit (MegaQuant™ Format)/K-FRGLMQ/60 assays per kit
TheD-FructoseandD-GlucoseMegaQuant™Formattestkitissuitablefor themeasurementandanalysisofD-fructoseandD-glucoseingrapes,grapejuiceandwineusingtheMegaQuant™colorimeter(measurementat505nm).
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Simplecolourimetricmethodforthedeterminationof
D-FructoseandD-Glucoseinfoodstuffs,beveragesand
othermaterials
Principle:
(hexokinase)
(1)D-Glucose+ATP→G-6-P+ADP
(hexokinase)
(2)D-Fructose+ATP→F-6-P+ADP
(phosphoglucoseisomerase)
(3)F-6-P ↔ G-6-P
(glucose-6-phosphatedehydrogenase)
(4)G-6-P+NADP+→gluconate-6-phosphate+NADPH+H+
(diaphorase)
(5)INT+NADPH+H+→NADP++INT-formazan
Kitsize: 60assays
Method: Spectrophotometricat505nm
Reactiontime: ~5min
Detectionlimit: 23.2mg/L
Applicationexamples:
Grapejuice/must,wine,beer,fruitjuices,softdrinks,milk,jam,honey,
dieteticfoods,bread,bakeryproducts,candies,desserts,confectionery,
ice-cream,fruitandvegetables,condiments,tobacco,cosmetics,
pharmaceuticals,paperandothermaterials(e.g.biologicalcultures,
samples,etc.)
Methodrecognition: Novelmethod
Advantages
- Novelproduct,patentedtechnology
- Spectrophotometer/laboratoryexpertisenotrequired
- Highlystablereagents(atleastthreeseasonsuse)
- Verycompetitiveprice(costpertest)
- Verysimpleprocedure
- Rapidreactiontime(~10min)
- Standardincluded