Megazyme/乳果糖分析试剂盒/K-乳酸/每个试剂盒50次分析
商品编号：
K-LACTUL
品牌：
Megazyme INC
市场价：
¥3880.00
美元价：
2328.00
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
TheLactuloseAssayKitissuitablefor thespecific,rapidandsensitivemeasurementandanalysisoflactuloseinmilkandmilk-basedsamples.ReagentsincludedinthiskitmayalsobepreparedforuseintheproceduredescribedbyISOMethod11285:2004.
Invitroandinvivoprotectiveeffectsoffermentedpreparationsofdietaryherbsagainstlipopolysaccharideinsult.
Bose,S.,Song,M.Y.,Nam,J.K.,Lee,M.J.&Kim,H.(2012).FoodChemistry,134(2),758-765.
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Lipopolysaccharide(LPS)isknowntoproduceendotoxicshockbytriggeringsystemicinflammatoryresponses.Here,weevaluatedtheprotectiveeffectsofthreefermented/re-fermentedherbs,RhizomaAtractylodisMacrocephalae,MassaMedicataFermentata,andDolichorisSemen,inanLPS-mediatedinflammatoryinsult,eitherindividuallyinvitrousingRAW264.7cellsorincombinationininvivousingrats.Ingeneral,eachofthefermentedherbsshowedappreciableinvitroanti-inflammatoryactivity,althoughthedegreeofthisactivityvariedwiththeherbused.Moreover,amixtureoffermentedherbalextractsincombinationwithprobioticssignificantlyattenuatedthebloodendotoxinandCRPlevels,aswellasthegutpermeABIlity,andsignificantlyaugmentedtheintestinalLactobacillusspp.colonisationinLPS-treatedrats.However,theseeffectswerenotobservedfollowingtheadmiNISTrationofthecorrespondingmixtureofunfermentedherbalextracts.Thus,ourresultshighlightthebeneficialimpactsoftheuseoffermentedherbproductswithprobioticstocombatLPS-mediatedinflammatoryinsults.
Theeffectofintestinalpermeabilityandendotoxemiaontheprognosisofacutepancreatitis.
Koh,Y.Y.,Jeon,W.K.,Cho,Y.K.,Kim,H.J.,Chung,W.G.,Chon,C.U.,Oh,T.Y.&Shin,J.H.(2012).GutandLiver,6(4),505-511.
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Background/Aims:Earlyintestinalmucosaldamageplaysanimportantroleinsevereacutepancreatitis(AP).Previousstudieshaveshownthatintestinalpermeability(IP),serumendotoxinandcytokinescontributetotheearlyintestinalbarrierdysfunctioninAP.ThisstudyexploredthepredictivecapacityofIP,endotoxemiaandcytokinesasprognosticindicatorsinAPpatients.Methods:Eighty-sevenAPpatientswereincludedinthestudy.ThepatientswereclassifiedintothreegroupsaccordingtotheBalthazarcomputedtomographyseverityindex(CTSI).Wecomparedthebiochemicalparameters,includingIP,serumendotoxinlevelandcytokinelevelamongthethreegroups.TheassociationsofIPwithserumendotoxin,cytokines,CTSI,andotherwidelyusedbiochemicalparametersandscoringsystemswerealsoexamined.Results:IP,serumendotoxin,interleukin(IL-6)andtumornecrosisfactor(TNF)-αhadapositivecorrelationwiththeCTSIofAP.Endotoxin,IL-6,TNF-α,CTSI,theRanson/APACHEIIscore,thedurationofhospitalstay,complicationsanddeathsignificantlyaffectIPintheAPpatients.Conclusions:WebelievethatIPwithsubsidiarymeasurementsofserumendotoxin,IL-6andTNF-αmaybereliableMarkersforpredictingtheprognosisofAP.FurtherstudiesthatcanrestoreandpreservegutbarrierfunctioninAPpatientsarewarranted.
Assessmentofgastrointestinalpermeabilitybylactulosetestinsheepafterrepeatedindomethacintreatment.
Minuti,A.,Ahmed,S.,Trevisi,E.,Piccioli-Cappelli,F.,Bertoni,G.&Bani,P.(2013).JournalofAnimalScience,91(12),5646-5653.
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Theaimofthestudywastoassessthesmallintestinepermeabilitybyusinglactuloseasasugarprobeandbloodmetabolitesinsheepafterachallengewithrepeatedindomethacininjections.Accordingtoachangeoverdesign,7adultsheep(4malesand3females)weresubjectedto4intramuscularinjections(every12h)ofsaline[control(CRT);7animals]orindomethacin(INDO;7animals).Twohoursafterthelastinjection,30goflactulosewereadministeredorallytobothCTRandINDO.Bloodsampleswerecollecteddailyfortheanalysisofthemetabolicprofileand5sampleswerecollectedat2-hintervalsfollowinglactuloseingestiontomonitorchangesinbloodlevelsoflactuloseasanindexofintestinalpermeability.TheINDOchallengeinducedclinicalsymptomssuchaslackofappetite,dullness,weakness,depression,anddiarrheawithtracesofbloodinthefeces.InINDOgroup,haptoglobinandceruloplasminincreased(P<0.01)=""after=""indo=""challenge=""whereas=""a=""decrease="">P<0.05)=""of=""negative=""acute=""phase=""reactants=""(e.g.,=""cholesterol,=""albumin,=""and=""paraoxonase)=""was=""observed.=""reactive=""oxygen=""metabolites=""increased="">P<0.01)=""from=""60=""to=""204=""h=""after=""the=""indo=""challenge=""start,=""with=""a=""decrease=""of=""vitamin=""e=""concentration=""from=""12="">P<0.01)=""to=""132=""h="">P<0.05).=""blood=""lactulose=""concentrations=""were=""increased="">P<0.05)=""in=""indo=""animals=""and=""the=""highest=""mean=""values=""(17.67=""µg/ml=""in=""indo=""vs.=""0.17=""µg/ml=""in=""crt;="">P<0.01)=""were=""observed=""6=""h=""after=""oral=""dosage=""of=""lactulose.=""these=""changes=""indicate=""that=""the=""indo=""challenge=""led=""to=""severe=""inflammatory=""responses=""with=""oxidative=""stress=""by=""enhancing=""small=""intestinal=""permeability=""in=""sheep=""that=""allowed=""lactulose=""to=""enter=""in=""blood.=""the=""results=""of=""this=""experiment=""demonstrate=""that=""lactulose=""can=""be=""used=""as=""a=""probe=""to=""assess=""gastrointestinal=""permeability=""in=""adult=""ruminants=""to=""test=""the=""consequences=""of=""stressing=""conditions=""on=""animal=""welfare.=""for=""this=""purpose,=""the=""most=""suitable=""time=""for=""blood=""sampling=""is=""between=""2=""and=""8=""h=""after=""the=""oral=""dosage=""of=""lactulose.="">
EvaluationoftheinvitroandinvivoprotectiveeffectsofunfermentedandfermentedRhizomacoptidisformulationsagainstlipopolysaccharideinsult.
Bose,S.,Jeon,S.,Eom,T.,Song,M.Y.&Kim,H.(2012).FoodChemistry,135(2),452-459.
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Lipopolysaccharide(LPS)canproduceendotoxicshockbytriggeringthesystemicinflammatoryresponse.Here,weevaluatedtheinvitroandinvivoprotectiveeffectsofunfermentedandfermentedRhizomacoptidis(RCandFRC,respectively)againstLPS-insult.Ingeneral,RCsuppressedtheLPS-inducedexpressionofkeyinflammatorymediatorsinRAW264.7cells,inadose-dependentmanner.Notably,FRCata20μg/mldoseincombinationwiththeprobioticusedforfermentationshowedmorepotentinvitroanti-inflammatoryactivitiesthanthatexhibitedbythecorrespondingdoseofRC.Moreover,oraltreatmentwithFRCinassociationwiththeprobiotic,butnotoraladministrationofRC,significantlyattenuatedbloodendotoxinandC-reactiveprotein(CRP)levels,andgutpermeability,andsignificantlyaugmentedtheintestinalpopulationofBifodobacteriumspp.andLactobacillusspp.inLPS-treatedanimals.OurresultsdemonstratethebeneficialimpactoffermentedRCincombinationwiththeassociatedprobioticincombatingLPS-insultbothinvitroandinvivo.
Theeffectsofco-administrationofprobioticswithherbalmedicineonobesity,metabolicendotoxemiaanddySBIosis:Arandomizeddouble-blindcontrolledclinicaltrial.
Lee,S.J.,Bose,S.,Seo,J.G.,Chung,W.S.,Lim,C.Y.&Kim,H.(2013).ClinicalNutrition,33(6),973-981.
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Backgrounds&aims:Probioticshelpmaintainbalanceincompositionofthegutmicrobiota,andhavebeenconsideredasapotentialtreatmentforobesity.Thisstudywasconductedinordertoassesstheeffectsofprobioticswhencombinedwithherbalmedicineintreatmentofobesity.Probioticsweretestedfortheabilitytomodulategutmicrobiota,gutpermeability,andendotoxinlevel,whichmayhavecorrelationwithfactorsinvolvedinobesity.Methods:Arandomized,double-blind,placebocontrolledstudywasconducted,inwhichpatientswithhigherBMI(>25kg/m2)andwaistcircumference(>85cm)wereenrolledandrandomlyassignedtoreceiveBofutsushosanwitheitherprobioticsorplacebocapsulesforaperiodofeightweeks.Assessmentofbodycompositionparameters,metabolicbiomarkers,endotoxinlevel,gutpermeability,andfecalbacteriainstoolwasperformedatbaselineandatweek8.ThestudywasregisteredattheClinicalResearchInformationService,approvedbytheKoreaNationalInstituteofHealth(KCT0000386).Results:Althoughbothgroupsshowedasignificantreductioninweightandwaistcircumference(p=0.000),nosignificantdifferencesinbodycompositionandmetabolicmarkerswereobserved.Incorrelationanalysis,changeinbodycompositionshowedpositivecorrelationwithendotoxinlevel(r=0.441,p<0.05=""for=""bw;=""and="">r=0.350,p<0.05=""for=""fat=""mass)=""and=""the=""population=""of=""gut="">Lactobacillusplantarum(r=0.425,p<0.05=""for=""bw;=""and="">r=0.407,p<0.05=""for=""bmi).=""the=""gram=""negative=""bacterial=""population=""in=""gut=""also=""exhibited=""positive=""correlation=""with=""changes=""in=""body=""composition=""(wc)=""and=""total=""cholesterol=""level="">r=0.359,and0.393,fortheformerandlaterparameters,respectively,p<0.05=""for=""both).=""while,=""the=""profile=""of=""gut="">Bifidobacteriumbrevepopulationshowednegativecorrelationwithendotoxinlevel(r=−0.350,p<0.05).="">Conclusions:Correlationsbetweengutmicrobiotaandchangeinbodycompositionindicatethatprobioticsmayinfluenceenergymetabolisminobesity.Correlationbetweenendotoxinlevelandweightreductionindicatesthatprobioticsmayplayanimportantroleinpreventionofendotoxinproduction,whichcanleadtogutmicrobiotadysbiosisassociatedwithobesity.
FlosLoniceraAmelioratesObesityandAssociatedEndotoxemiainRatsthroughModulationofGutPermeabilityandIntestinalMicrobiota.
Wang,J.H.,Bose,S.,Kim,G.C.,Hong,S.U.,Kim,J.H.,Kim,J.E.&Kim,H.(2014).PloSone,9(1),e86117.
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BackgroundandAims:Increasingevidencehasindicatedacloseassociationofhost-gutflorametabolicinteractionwithobesity.FlosLonicera,atrADItionalherbalmedicine,isusedwidelyineasternAsiaforthetreatmentofvariousdisorders.TheaimofthisstudywastoevaluatewhetherunfermentedorfermentedformulationsofFlosLoniceracouldexertabeneficialimpacttocombatobesityandrelatedmetabolicendotoxemia.Methods:Obesityandmetabolicendotoxemiawereinducedseparatelyortogetherinratsthroughfeedingaeight-weekhighfatdieteitheralone(HFDcontrolgroup)orincombinationwithasingleLPSstimulation(intraperitonealinjection,0.75mg/kg)(LPScontrolgroup).While,themechanismofactionoftheLoniceraformulationswasexploredinvitrousingRAW264.7andHCT116celllinesasmodels.Results:Incell-basedstudies,treatmentwithbothunfermentedFlosLonicera(UFL)andfermentedFlosLonicera(FFL)formulationsresultedinsuppressionofLPS-inducedNOproductionandgeneexpressionofvitalproinflammatorycytokines(TNF-α,COX-2,andIL-6)inRAW264.7cells,reducedthegeneexpressionofzonulaoccludens(ZO)-1andclaudin-1,andnormalizedtransepithelialelectricresistance(TEER)andhorseradishperoxidase(HRP)fluxinLPS-treatedHCT-116cells.Inananimalstudy,treatmentofHFDaswellasHFD+LPSgroupswithUFLorFFLresultedinanotabledecreaseinbodyandadiposetissueweights,amelioratedtotalcholesterol,HDL,triglyceride,aspartatetransaminaseandendotoxinlevelsinserum,reducedtheurinarylactulose/mannitolratio,andmarkedlyalleviatedlipidaccumulationinliver.Inaddition,exposureofHFDaswellasHFD+LPSgroupswithUFLorFFLresultedinsignificantalterationofthedistributionofintestinalflora,especiallyaffectingthepopulationofAkkermansiaspp.andratioofBacteroidetesandFirmicutes.Conclusion:ThisevidencecollectivelydemonstratesthatFlosLoniceraamelioratesobesityandrelatedmetabolicendotoxemiaviaregulatingdistributionofgutfloraandgutpermeability.
HepatoprotectiveeffectofLentinusedodesmyceliafermentedformulationagainstalcoholicliverinjuryinrats.
Chung,W.S.,Wang,J.H.,Bose,S.,Park,J.M.,Park,S.O.,Lee,S.J.,Jeon,S.&Kim,H.(2015).JournalofFoodBiochemistry,39(3),251-262.
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Thehepatoprotectiveeffectsoffermentedblackricebranextracts(FF1andFF2:blackricebranfermentedbyLentinusedodesderivedfrommyceliumsupplementedwithsoybeanor Hoveniadulcis)andtheirassociatedmechanismswereevaluated.Inaninvitroexperiment,FFscausedsignificantameliorationofthemetabolicfunctionofrathepatocytestreatedwith NH4Cl.Inaddition,administrationofFFstoratswithchronicliverinjuryinducedby12-weekcontinualalcoholconsumptionresultedinsignificantrestorationofbodyweightshrinkage,notableattenuationofexcessiveaspartateaminotransferase,alkalinephosphataseandendotoxininserum,malondialdehydeinliverandthelactulose/mannitolratioinurine.FurThermore,FF1orFF2alsocausedsignificantdownregulationofgeneexpressionofseveralcriticalinflammatorymediators(interleukin-6,tumornecrosisfactor-alpha,cyclooxygenase-2andinducIBLenitricoxidesynthase).HistopathologicalfindingsalsoindicatedthatFFsreducedinflammation,necrosisandfattyinfiltrationinlivertissue.Takentogether,FFsexerthepatoprotectiveeffectsthroughanti-inflammatoryandanti-lipidperoxidativepropertiesandregulationofintestinalpermeability.
Evaluationoffurosine,lactuloseandacid-solubleβ-lactoglobulinastimetemperatureintegratorsforwhippingcreamsamplesatretailinAustria.
Boitz,L.I.&Mayer,H.K.(2015).InternationalDairyJournal,50,24-31.
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Threeheatloadindicators,i.e.,furosine,lactuloseandacid-solubleβ-lactoglobulin,weredeterminedinwhippingcreamsamplespurchasedfromAustrianmarket(n = 33),aswellascommercialsamplesobtaineddirectlyfromoneAustriandairycompany(onebrand,n= 25).Furosinecontentswere47.8 ± 14.0,72.2 ± 36.6,and172.5 ± 17.7 mg 100 g−1protein,andβ-lactoglobulincontentswere143 ± 91,195 ± 150,and89 ± 31 mg L−1forretailedpasteurised,extendedshelflife(ESL),andultra-hightemperature(UHT)creamsamples,respectively.Lactuloseconcentrations(analysedenzymatically)were29 ± 10,56 ± 41,and201 ± 24 mg L−1forpasteurised,ESLandUHTcreamsamples,respectively.Linearcorrelationobtainedforfurosineandlactuloseconcentrationsindicatedthattheseindicatorscanreliablyassesstheheatloadofpasteurised,ESLandUHTcreamsamples,whereasβ-lactoglobulinwasdefinitelynotappropriatetodiscriminatebetweentheseheatingcategories.
UV-methodforthedeterminationofLactuloseinmilkand
foodstuffscontainingdairyproducts
Principle:
(β-galactosidase)
(1)Lactulose+H2O→D-galactose+D-fructose
(glucoseoxidase+catalase+H2O2)
(2)D-Glucose+H2O+O2→D-gluconicacid+H2O2
(hexokinase)
(3)D-Fructose+ATP→F-6-P+ADP
(phosphoglucoseisomerase)
(4)F-6-P → G-6-P
(glucose-6-phosphatedehydrogenase)
(5)G-6-P+NADP+→gluconate-6-phosphate+NADPH+H+
(gluconate-6-phosphatedehydrogenase)
(6)Gluconate-6-phosphate+NADP+→ribulose-5-phosphate+NADPH
+CO2+H+
Kitsize: 50assays
Method: Spectrophotometricat340nm
Reactiontime: ~120min
Detectionlimit: 4.8mg/L
Applicationexamples:
Milk,dairyproductsandfoodscontainingmilk
Methodrecognition: Novelmethod
Advantages
- Twicethesensitivityoftraditionalhexokinasebasedlactulosemethods
- Verycosteffective
- Allreagentsstablefor>2yearsafterpreparation
- Mega-Calc™softwaretoolisavailablefromourwebsiteforhassle-freerawdataprocessing
- Standardincluded
品牌介绍
Megazyme品牌产品简介
来源：作者：人气：2149发表时间：2016-05-19 10:59:00【大 中 小】
Megazyme是一家全球性公司，专注于开发和提供用于饮料、谷物、乳制品、食品、饲料、发酵、生物燃料和葡萄酒产业用的分析试剂、酶和检测试剂盒。Megazyme的许多检测试剂盒产品已经为众多官方科学协会（包括AOAC, AACC , RACI, EBC和ICC等），经过严格的审核，批准认证为官方标准方法，确保以准确、可靠、定量和易于使用的测试方法，满足客户的质量诉求。
Megazyme的主要产品线包括：
◆ 检测试剂盒
◆ 酶
◆ 酶底物
◆ 碳水化合物
◆ 化学品/仪器
官网地址：http://www.megazyme.com
检测试剂盒特色产品：
货号
中文品名
用途
K-ACETAF
乙酸[AF法]检测试剂盒
酶法定量分析乙酸最广泛使用的方法
K-ACHDF
可吸收糖/膳食纤维检测试剂盒
酒精沉淀法测定膳食纤维
K-AMIAR
氨快速检测试剂盒
用于包括葡萄汁、葡萄酒以及其它食品饮料样品中氨含量的快速检测分析。
K-AMYL
直链淀粉/支链淀粉检测试剂盒
谷物淀粉和而粉中直链淀粉/支链淀粉比例和含量检测
K-ARAB
阿拉伯聚糖检测试剂盒
果汁浓缩液中阿拉伯聚糖的检测
K-ASNAM
L-天冬酰胺/L-谷氨酰胺和氨快速检测试剂盒
用于食品工业中丙烯酰胺前体、细胞培养基、以及上清液组分中、L-天冬酰胺，谷氨酰胺和氨的检测分析
K-ASPTM
阿斯巴甜检测试剂盒
专业用于测定饮料和食品中阿斯巴甜含量，操作简单
K-BETA3
β-淀粉酶检测试剂盒
适用于麦芽粉中β-淀粉酶的测定
K-BGLU
混合键β-葡聚糖检测试剂盒
测定谷物、荞麦粉、麦汁、啤酒及其它食品中混合键β-葡聚糖(1,3:1,4-β-D-葡聚糖)的含量
K-CERA
α-淀粉酶检测试剂盒
谷物和发酵液（真菌和细菌）中α-淀粉酶的分析测定
K-CITR
柠檬酸检测试剂盒
快速、可靠地检测食品、饮料和其它物料中柠檬酸（柠檬酸盐）含量
K-DLATE
乳酸快速检测试剂盒
快速、特异性检测饮料、肉类、奶制品和其它食品中L-乳酸和D-乳酸(乳酸盐）含量
K-EBHLG
酵母β-葡聚糖酶检测试剂盒
用于测量和分析酵母中1,3:1,6?-β-葡聚糖，也可以检测1,3-葡聚糖
K-ETSULPH
总亚硫酸检测试剂盒
测定葡萄酒、饮料、食品和其他物料中总亚硫酸含量（按二氧化硫计）的一种简单，高效，可靠的酶法检测方法
K-FRGLMQ
D-果糖/D-葡萄糖[MegaQuant法]检测试剂盒
适用于使用megaquant?色度计（505nm下）测定葡萄、葡萄汁和葡萄酒中D-果糖和D-葡萄糖的含量。
K-FRUC
果聚糖检测试剂盒
含有淀粉、蔗糖和其他糖类的植物提取物和食品中果聚糖的含量测定。
K-FRUGL
D-果糖/D-葡萄糖检测试剂盒
对植物和食品中果糖或葡萄糖含量的酶法紫外分光测定。
K-GALM
半乳甘露聚糖检测试剂盒
食品和植物产品中半乳甘露聚糖的含量检测
K-GLUC
D-葡萄糖[GOPOD]检测试剂盒
谷物提取物中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUHK
D-葡萄糖[HK]检测试剂盒
植物和食品中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUM
葡甘聚糖检测试剂盒
植物和食品中葡甘聚糖的含量测定。
K-INTDF
总膳食纤维检测试剂盒
总膳食纤维特定检测和分析
K-LACGAR
乳糖/D-半乳糖快速检测试剂盒
用于快速检测食品和植物产品中乳糖、D-半乳糖和L-阿拉伯糖
K-LACSU
乳糖/蔗糖/D-葡萄糖检测试剂盒
混合面粉和其它物料中蔗糖、乳糖和D-葡萄糖的测定
K-LACTUL
乳果糖检测试剂盒
特异性、快速和灵敏测量奶基样品中乳果糖含量
K-MANGL
D-甘露糖/D-果糖/D-葡萄糖检测试剂盒
适合测定植物产品和多糖酸性水解产物中D-甘露糖含量
K-MASUG
麦芽糖/蔗糖/D-葡萄糖检测试剂盒
在植物和食品中麦芽糖，蔗糖和葡萄糖的含量检测
K-PECID
胶质识别检测试剂盒
食品配料中果胶的鉴别
K-PHYT
植酸(总磷)检测试剂盒
食品和饲料样品植酸/总磷含量测量的简便方法。不需要通过阴离子交换色谱对植酸纯化，适合于大量样本分析
K-PYRUV
丙酮酸检测试剂盒
在啤酒、葡萄酒、果汁、食品和体液中丙酮酸分析
K-RAFGA
棉子糖/D-半乳糖检测试剂盒
快速测量植物材料和食品中棉子糖和半乳糖含量
K-RAFGL
棉子糖/蔗糖/D-半乳糖检测试剂盒
分析种子和种子粉中D-葡萄糖、蔗糖、棉子糖、水苏糖和毛蕊花糖含量。通过将棉子糖、水苏糖和毛蕊花糖酶解D-葡萄糖、D-果糖和半乳糖，从而测定葡萄糖含量来确定
K-SDAM
淀粉损伤检测试剂盒
谷物面粉中淀粉损伤的检测和分析
K-SUCGL
蔗糖/D-葡萄糖检测试剂盒
饮料、果汁、蜂蜜和食品中蔗糖和葡萄糖的分析
K-SUFRG
蔗糖/D-果糖/D-葡萄糖检测试剂盒
适用于植物和食品中蔗糖、D-葡萄糖和D-果糖的测定
K-TDFR
总膳食纤维检测试剂盒
总膳食纤维检测
K-TREH
海藻糖检测试剂盒
快速、可靠地检测食品、饮料和其它物料中海藻糖含量
K-URAMR
尿素/氨快速检测试剂盒
适用于水、饮料、乳制品和食品中尿素和氨的快速测定
K-URONIC
D-葡萄糖醛酸/D-半乳糖醛酸检测试剂盒
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