Megazyme/棉子糖/D-半乳糖检测试剂盒/K-RAFGA/每个试剂盒120次检测
商品编号：
K-RAFGA
品牌：
Megazyme INC
市场价：
¥4860.00
美元价：
2916.00
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
TheRaffinose/D-Galactosetestkitisspecificandarapid measurementandanalysisofraffinoseandD-galactoseinplantmaterialsandfoodproducts.
Measurementoftotalstarchincerealproductsbyamyloglucosidase-alpha-amylasemethod:collaborativestudy.
McCleary,B.V.,Gibson,T.S.&Mugford,D.C.(1997).JournalofAOACInternational,80,571-579.
LinktoArticle
ReadAbstract
AnAmericanAssociationofCerealChemists/AOACcollaborativestudywasconductedtoevaluatetheaccuracyandreliABIlityofanenzymeassaykitprocedureformeasurementoftotalstarchinarangeofcerealgrainsandproducts.Thefloursampleisincubatedat95degreesCwithThermostablealpha-amylasetocatalyzethehydrolysisofstarchtomaltodextrins,thepHoftheslurryisadjusted,andtheslurryistreatedwithahighlypurifiedamyloglucosidasetoquantitativelyhydrolyzethedextrinstoglucose.Glucoseismeasuredwithglucoseoxidase-peroxidasereagent.Thirty-twocollaboratorsweresent16homogeneoustestsamplesas8blindduplicates.Thesesamplesincludedchickenfeedpellets,whitebread,greenpeas,high-amylosemaizestarch,whitewheatflour,wheatstarch,oatbran,andspaghetti.Allsampleswereanalyzedbythestandardprocedureasdetailedabove;4samples(high-amylosemaizestarchandwheatstarch)werealsoanalyzedbyamethodthatrequiresthesamplestobecookedfirstindimethylsulfoxide(DMSO).Relativestandarddeviationsforrepeatability(RSD(r))rangedfrom2.1to3.9%,andrelativestandarddeviationsforreproducibility(RSD(R))rangedfrom2.9to5.7%.TheRSD(R)valueforhighamylosemaizestarchanalyzedbythestandard(non-DMSO)procedurewas5.7%;thevaluewasreducedto2.9%whentheDMSOprocedurewasused,andthedeterminedstarchvaluesincreasedfrom86.9to97.2%.
Measurementofcarbohydratesingrain,feedandfood.
McCleary,B.V.,Charnock,S.J.,Rossiter,P.C.,O’Shea,M.F.,Power,A.M.&Lloyd,R.M.(2006).JournaloftheScienceofFoodandAgriculture,86(11),1648-1661.
LinktoArticle
ReadAbstract
Proceduresforthemeasurementofstarch,starchdamage(gelatinisedstarch),resistantstarchandtheamylose/amylopectincontentofstarch,β-glucan,fructan,glucomannanandgalactosyl-sucroseoligosaccharides(raffinose,stachyoseandverbascose)inplantmaterial,animalfeedsandfoodsaredescribed.Mostofthesemethodshavebeensuccessfullysubjectedtointerlaboratoryevaluation.AllmethodsarebasedontheuseofenzymeseitherpurifiedbyconventionalchromatographyorproducedusingmolecularBIOLOGytechniques.Suchmethodsallowspecific,accurateandreliablequantificationofaparticularcomponent.Problemsincalculatingtheactualweightofgalactosyl-sucroseoligosaccharidesintestsamplesarediscussedindetail.
Acidicα-galactosidaseisthemostabundantnectarininfloralnectarofcommontobacco(Nicotianatabacum).
Zha,H.G.,Flowers,V.L.,Yang,M.,Chen,L.Y.&Sun,H.(2012).Annalsofbotany,109(4),735-745.
LinktoArticle
ReadAbstract
BackgroundandAims:Todate,mostfloralnectarins(nectarproteins)arereportedtofunctioninnectardefence,particularlyforinsect-pollinatedoutcrossingspecies.Wecomparednectarincompositionandabundanceinselfingcommontobacco(Nicotianatobaccum)withoutcrossingornamentaltobaccoplantstoelucidatethefunctionaldifferenceofnectarinsindifferentreproductivesystems.Methods:Commontobacco(CT)nectarinswereseparatedbySDS-PAGEandtheNterminusofthemostabundantnectarinwassequencedviaEdmandegradation.Thefull-lengthnectaringenewasamplifiedandclonedfromgenomicDNAandmRNAwithhiTail-PCRandRACE(rapidamplificationofCDNAends),andexpressionpatternsweretheninvestigatedindifferenttissuesusingsemi-quantitativereversetranscriptasePCR.Additionally,high-performanceliquidchromatographyandenzymaticanalysesofnectarsugarcomposition,andotherbiochemicaltraitsandfunctionsofthenovelnectarinwerestudied.KeyResults:ThemostabundantnectarininCTnectarisanacidicα-galactosidase,heredesignatedNTα-Gal.Thiscompoundhasamolecularmassof40013DaandatheoreticalpIof5•33.NTα-Galhasaconservedα-Galcharacteristicsignature,encodesamatureproteinof364aminoacidsandisexpressedindifferentorgans.Comparedwith27othermelliferousplantspeciesfromdifferentfamilies,CTfloralnectardemonstratedthehighestα-Galactivity,whichisinhibitedbyD-galactose.RaffinosefamilyoligosaccharideswerenotdetectedinCTnectar,indicatingthatNTα-Galdoesnotfunctioninpost-secretoryhydrolysis.Moreover,tobaccoplantfruitsdidnotdevelopintactskinwithgalactoseinhibitionofNTα-Galactivityinnectar,suggestingthatNTα-Galinducescell-wallsurfacerestructuringduringtheinitialstagesoffruitdevelopment.Conclusions:α-GalwasthemostabundantnectarininselfingCTplants,butwasnotdetectedinthenectarofstrictlyoutcrossingsistertobaccospecies.Nofunctionwasdemonstratedinantimicrobialdefence.Therefore,floralnectarinsinselfingspeciesmaintaintheirfunctionalsignificanceinreproductiveorgandevelopment.
ViscozymeLactiononsoyslurryaffectscarbohydratesandantioxidantpropertiesofsilkentofu.
Rosset,M.,Prudencio,S.H.&Beléia,A.D.P.(2012).FoodScienceandTechnologyInternational,18(6),531-538.
LinktoArticle
ReadAbstract
ThisstudyinvestigatedtheenzymatictreatmentofsoyslurryusingViscozymeLtohydrolyzethecarbohydrates.TheoptimumtemperatureofViscozymeLactionwas55°C.Theincreaseofglucoseandgalactosecontentintofu(1.36and0.19 g/100 g,respectively)confirmedtheViscozymeactivityonsoyslurrywhencomparedtothecontrol.Thetreatedtofuhadmoretotalphenolicsthanthecontrol(173and161 mggallicacidequivalents/100 gfreeze-driedtofu,respectively)andhigherantioxidantactivitybythe2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonicacid)diammoniumsaltand1,1-diphenyl-2-picryhydrazyl,2,2-diphenyl-1-picryhydrazylrADIcaltests.Totalreducingsugar(glucoseequivalents)contentintreatedtofuwasapproximatelyfourtimeshigherthanthatinthecontrolundertheoptimumconditions(30FungalBeta-Glucanaseunits/10 gsolids,55°C,30 min).Thetofusdifferedinthesensoryanalysisforsoyodorandsurfaceuniformity,buttherewasnopreferenceforoneovertheother.
EffectsofabrownbeanseveningmealonmetabolicriskMarkersandappetiteregulatinghormonesatasubsequentstandardizedbreakfast:arandomizedcross-overstudy.
Nilsson,A.,Johansson,E.,Ekström,L.&Björck,I.(2013).PloSone,8(4),e59985.
LinktoArticle
ReadAbstract
Background:Dietarypreventionstrategiesareincreasinglyrecognizedasessentialtocombatthecurrentepidemicofobesityandrelatedmetabolicdisorders.ThepurposeofthepresentstudywastoevaluatethepotentialprebioticeffectsofindigestIBLecarbohydratesinSwedishbrownbeans(Phaseolusvulgarisvar.nanus)inrelationtocardiometabolicriskmarkersandappetiteregulatinghormones.Methods:Brownbeans,orwhitewheatbread(WWB,referenceproduct)wereprovidedaseveningmealsto16healthyyoungadultsinarandomisedcrossoverdesign.Glucose,insulin,appetiteregulatoryhormones,GLP-1,GLP-2,appetitesensations,andmarkersofinflammationweremeasuredatafollowingstandardisedbreakfast,thatisat11to14hposttheeveningmeals.Additionally,colonicfermentationactivitywasestimatedfrommeasurementofplasmashortchainfattyacids(SCFA,includingalsobranchedchainfattyacids)andbreathhydrogen(H2)excretion.Results:Aneveningmealofbrownbeans,incomparisonwithWWB,loweredbloodglucose(−15%,p<0.01)- and="" insulin="" (−16%,="">p<0.05) responses,="" increased="" satiety="" hormones="" (pyy="" 51%,="">p<0.001), suppressed="" hunger="" hormones="" (ghrelin="" −14%,="">p<0.05), and="" hunger="" sensations="" (−15%,="">p=0.05),increasedGLP-2concentrations(8.4%,p<0.05) and="" suppressed="" inflammatory="" markers="" (il-6="" −35%,="" and="" il-18="" −8.3%,="">p<0.05) at="" a="" subsequent="" standardised="" breakfast.="" breath="">2(141%,p<0.01), propionate="" (16%,="">p<0.05), and="" isobutyrate="" (18%,="">p<0.001) were="" significantly="" increased="" after="" brown="" beans="" compared="" to="" after="" wwb,="" indicating="" a="" higher="" colonic="" fermentative="" activity="" after="" brown="" beans.="">Conclusions:Aneveningmealwithbrownbeansbeneficiallyaffectedimportantmeasuresofcardiometabolicriskandappetiteregulatoryhormones,withinatimeframeof11–14h,incomparisontoaWWBeveningmeal.ConcentrationsofplasmaSCFAandH2wereincreased,indicatinginvolvementofcolonicfermentation.Indigestiblecolonicsubstratesfrombrownbeansmayprovideapreventivetoolinrelationtoobesityandthemetabolicsyndrome.
Theeffectsoffermentationandenzymatictreatmentofpeaonnutrientdigestibilityandgrowthperformanceofbroilers.
Boroojeni,F.G.,Senz,M.,Kozłowski,K.,Boros,D.,Wisniewska,M.,Rose,D.,Männer,K.&Zentek,J.(2017).Animal,1-10.
LinktoArticle
ReadAbstract
Thepresentstudyexaminedtheimpactsofnative,fermentedorenzymaticallytreatedpeas(PisumsativumL.)inclusioninbroilerdiets,ongrowthperformanceandnutrientdigestibility.Forthefermentationprocess,Madonnapeawasmixedwithwater(1/1)containing2.57×108Bacillussubtilis(GalliPro®)spores/kgpeaandthen,incubatedfor48hat30°C.Fortheenzymatictreatmentprocess,theusedwaterfordoughproductioncontainedthreeenzymes,AlphaGalTM (α-galactosidase),RONOZYME®ProActandVP(proteaseandpectinasesrespectively–DSM,Switzerland)andthepeadoughincubatedfor24hat30°C.Ninecorn-wheat-soybeandietswereformulatedbysupplying10%,20%and30%oftherequiredCPwitheithernative,fermentedorenzymaticallytreatedpeas.Performancewasrecordedweeklyandattheendoftheexperiment(day35),apparentilealdigestibility(AID)ofCP,aminoacids(AA),crudefat,starch,Ca,PandKweredetermined.DataweresubjectedtoANOVAusingGLMprocedurewitha3×3factorialarrangementoftreatments.Bothprocessesreducedα-galactosides,phytate,trypsininhibitoractivityandresistantstarchinpeas.Increasinglevelsofpeaproductsupto300g/kgdiet,reducedBWgainandfeedintake(P≤0.05).Broilersfeddietscontainingenzymaticallytreatedpeahadthebestfeedconversionratioatday35.DifferenttypesofpeaproductandtheirinclusionlevelshadnoeffectonAIDofallnutrients.TheinteractionbetweentypeofthepeaproductsandinclusionlevelswassignificantforAIDofstarch.Fornativepeadiets,10%groupshowedsimilarAIDofstarchto20%nativepeabutithadhigherAIDthan30%nativepea.Forfermentedandenzymaticallytreatedgroups,allthreelevelsdisplayedsimilarAIDofstarch.Inconclusion,enzymatictreatmentandfermentationcouldimprovethenutritionalqualityofpea.Inclusionofenzymaticallytreatedpeainbroilerdietscouldimprovebroilerperformancecomparedwithotherpeaproductswhile,itdisplayedneitherpositivenornegativeimpactonnutrientdigestibility.Thepresentfindingsindicatethefeasibilityoftheseprocesses,particularlyenzymatictreatment,forimprovingthenutritionalqualityofpeaasaproteinsourceforbroilernutrition.
UV-methodforthedeterminationofRaffinose(alsostachyose
andverbascose)andD-Galactoseinlegumeseeds,plant
materials,foodstuffsandfeed
Principle:
(α-galactosidase)
(1)Raffinose+stachyose+verbascose+H2O→D-galactose+
sucrose
(galactosemutarotase)
(2)α-D-Galactose↔β-D-galactose
(β-galactosedehydrogenase)
(3)β-D-Galactose+NAD+→D-galactonicacid+NADH+H+
Kitsize: *120assays
* Thenumberofmanualtestsperkitcanbedoubledifallvolumesarehalved.
ThiscanbereadilyaccommodatedusingtheMegaQuantTM Wave
Spectrophotometer(D-MQWAVE).Method: Spectrophotometricat340nm
Reactiontime: ~60min
Detectionlimit: 21mg/L
Applicationexamples:
Cerealflours,soybeanflour,by-productsofsucrosemanufacture
andothermaterials
Methodrecognition:
Usedandacceptedinfoodanalysis
Advantages
- Veryrapidreactionduetoinclusionofgalactosemutarotase(patentedtechnology)
- Verycompetitiveprice(costpertest)
- Allreagentsstablefor>2yearsafterpreparation
- Mega-Calc™softwaretoolisavailablefromourwebsiteforhassle-freerawdataprocessing
- Standardincluded
品牌介绍
Megazyme品牌产品简介
来源：作者：人气：2149发表时间：2016-05-19 10:59:00【大 中 小】
Megazyme是一家全球性公司，专注于开发和提供用于饮料、谷物、乳制品、食品、饲料、发酵、生物燃料和葡萄酒产业用的分析试剂、酶和检测试剂盒。Megazyme的许多检测试剂盒产品已经为众多官方科学协会（包括AOAC, AACC , RACI, EBC和ICC等），经过严格的审核，批准认证为官方标准方法，确保以准确、可靠、定量和易于使用的测试方法，满足客户的质量诉求。
Megazyme的主要产品线包括：
◆ 检测试剂盒
◆ 酶
◆ 酶底物
◆ 碳水化合物
◆ 化学品/仪器
官网地址：http://www.megazyme.com
检测试剂盒特色产品：
货号
中文品名
用途
K-ACETAF
乙酸[AF法]检测试剂盒
酶法定量分析乙酸最广泛使用的方法
K-ACHDF
可吸收糖/膳食纤维检测试剂盒
酒精沉淀法测定膳食纤维
K-AMIAR
氨快速检测试剂盒
用于包括葡萄汁、葡萄酒以及其它食品饮料样品中氨含量的快速检测分析。
K-AMYL
直链淀粉/支链淀粉检测试剂盒
谷物淀粉和而粉中直链淀粉/支链淀粉比例和含量检测
K-ARAB
阿拉伯聚糖检测试剂盒
果汁浓缩液中阿拉伯聚糖的检测
K-ASNAM
L-天冬酰胺/L-谷氨酰胺和氨快速检测试剂盒
用于食品工业中丙烯酰胺前体、细胞培养基、以及上清液组分中、L-天冬酰胺，谷氨酰胺和氨的检测分析
K-ASPTM
阿斯巴甜检测试剂盒
专业用于测定饮料和食品中阿斯巴甜含量，操作简单
K-BETA3
β-淀粉酶检测试剂盒
适用于麦芽粉中β-淀粉酶的测定
K-BGLU
混合键β-葡聚糖检测试剂盒
测定谷物、荞麦粉、麦汁、啤酒及其它食品中混合键β-葡聚糖(1,3:1,4-β-D-葡聚糖)的含量
K-CERA
α-淀粉酶检测试剂盒
谷物和发酵液（真菌和细菌）中α-淀粉酶的分析测定
K-CITR
柠檬酸检测试剂盒
快速、可靠地检测食品、饮料和其它物料中柠檬酸（柠檬酸盐）含量
K-DLATE
乳酸快速检测试剂盒
快速、特异性检测饮料、肉类、奶制品和其它食品中L-乳酸和D-乳酸(乳酸盐）含量
K-EBHLG
酵母β-葡聚糖酶检测试剂盒
用于测量和分析酵母中1,3:1,6?-β-葡聚糖，也可以检测1,3-葡聚糖
K-ETSULPH
总亚硫酸检测试剂盒
测定葡萄酒、饮料、食品和其他物料中总亚硫酸含量（按二氧化硫计）的一种简单，高效，可靠的酶法检测方法
K-FRGLMQ
D-果糖/D-葡萄糖[MegaQuant法]检测试剂盒
适用于使用megaquant?色度计（505nm下）测定葡萄、葡萄汁和葡萄酒中D-果糖和D-葡萄糖的含量。
K-FRUC
果聚糖检测试剂盒
含有淀粉、蔗糖和其他糖类的植物提取物和食品中果聚糖的含量测定。
K-FRUGL
D-果糖/D-葡萄糖检测试剂盒
对植物和食品中果糖或葡萄糖含量的酶法紫外分光测定。
K-GALM
半乳甘露聚糖检测试剂盒
食品和植物产品中半乳甘露聚糖的含量检测
K-GLUC
D-葡萄糖[GOPOD]检测试剂盒
谷物提取物中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUHK
D-葡萄糖[HK]检测试剂盒
植物和食品中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUM
葡甘聚糖检测试剂盒
植物和食品中葡甘聚糖的含量测定。
K-INTDF
总膳食纤维检测试剂盒
总膳食纤维特定检测和分析
K-LACGAR
乳糖/D-半乳糖快速检测试剂盒
用于快速检测食品和植物产品中乳糖、D-半乳糖和L-阿拉伯糖
K-LACSU
乳糖/蔗糖/D-葡萄糖检测试剂盒
混合面粉和其它物料中蔗糖、乳糖和D-葡萄糖的测定
K-LACTUL
乳果糖检测试剂盒
特异性、快速和灵敏测量奶基样品中乳果糖含量
K-MANGL
D-甘露糖/D-果糖/D-葡萄糖检测试剂盒
适合测定植物产品和多糖酸性水解产物中D-甘露糖含量
K-MASUG
麦芽糖/蔗糖/D-葡萄糖检测试剂盒
在植物和食品中麦芽糖，蔗糖和葡萄糖的含量检测
K-PECID
胶质识别检测试剂盒
食品配料中果胶的鉴别
K-PHYT
植酸(总磷)检测试剂盒
食品和饲料样品植酸/总磷含量测量的简便方法。不需要通过阴离子交换色谱对植酸纯化，适合于大量样本分析
K-PYRUV
丙酮酸检测试剂盒
在啤酒、葡萄酒、果汁、食品和体液中丙酮酸分析
K-RAFGA
棉子糖/D-半乳糖检测试剂盒
快速测量植物材料和食品中棉子糖和半乳糖含量
K-RAFGL
棉子糖/蔗糖/D-半乳糖检测试剂盒
分析种子和种子粉中D-葡萄糖、蔗糖、棉子糖、水苏糖和毛蕊花糖含量。通过将棉子糖、水苏糖和毛蕊花糖酶解D-葡萄糖、D-果糖和半乳糖，从而测定葡萄糖含量来确定
K-SDAM
淀粉损伤检测试剂盒
谷物面粉中淀粉损伤的检测和分析
K-SUCGL
蔗糖/D-葡萄糖检测试剂盒
饮料、果汁、蜂蜜和食品中蔗糖和葡萄糖的分析
K-SUFRG
蔗糖/D-果糖/D-葡萄糖检测试剂盒
适用于植物和食品中蔗糖、D-葡萄糖和D-果糖的测定
K-TDFR
总膳食纤维检测试剂盒
总膳食纤维检测
K-TREH
海藻糖检测试剂盒
快速、可靠地检测食品、饮料和其它物料中海藻糖含量
K-URAMR
尿素/氨快速检测试剂盒
适用于水、饮料、乳制品和食品中尿素和氨的快速测定
K-URONIC
D-葡萄糖醛酸/D-半乳糖醛酸检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中六元糖醛酸含量（D-葡萄糖醛酸和D-半乳糖醛酸）
K-XYLOSE
D-木糖检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中D-木糖含量
K-YBGL
Beta葡聚糖[酵母和蘑菇]检测试剂盒
检测酵母和蘑菇制品中1,3:1,6-beta-葡聚糖和α-葡聚糖含量
联络我们
