EnzymaticYeastBeta-Glucantestkit,anenzymaticprocedureforthemeasurementandanalysisof1,3:1,6-β-glucaninyeast.Alsomeasures1,3-β-glucan.
InVitropotentialantioxidantactivityof(1→3),(1→6)-β-D-glucanandproteinfractionsfromSaccharomycescerevisiaecellwalls.
TriggeringDectin-1-PathwayAloneIsNotSufficienttoInduceCytokineProductionbyMurineMacrophages.
Quantificationof1,3-β-D-glucanfromyeastaddedasafunctionalingredienttobread.
Colourimetricmethodfortheenzymaticdeterminationof
yeastβ-Glucanandalso(1-3)-βGlucans
Principle:
(KOH,4°C,30min)
(1)1,3:1,6-β-Glucan+1,3-β-glucan+H2O→solubleglucan
(glucazyme,40°C,16h)
(2)Solubleglucan+H2O→D-glucose
(glucoseoxidase)
(3)D-Glucose+H2O+O2→D-gluconate+H2O2
(peroxidase)
(4)2H2O2+p-hydroxybenzoicacid+4-aminoantipyrine→
quinoneimine+4H2O
Kitsize: 50assays
Method: Spectrophotometricat510nm
Reactiontime: ~100min
Detectionlimit: 1-100%ofsampleweight
Applicationexamples:
Yeastpreparationsandothermaterials
Methodrecognition: Novelmethod
Advantages
- Verycompetitiveprice(costpertest)
- Allreagentsstablefor>12monthsafterpreparation
- Mega-Calc™softwaretoolisavailablefromourwebsiteforhassle-freerawdataprocessing
- Standardincluded