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Megazyme/β-Glucan Assay Kit (Yeast & Mushroom) /K-YBGL/100 assays per kit
Megazyme/β-Glucan Assay Kit (Yeast & Mushroom) /K-YBGL/100 assays per kit
商品编号：
K-YBGL
品牌：
Megazyme INC
市场价：
¥4340.00
美元价：
2604.00
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
β-Glucan(Yeast&Mushroom)AssayKitissuitablefor themeasurementandanalysisof1,3:1,6-β-glucanandα-glucaninyeastandmushroom preparations.
New,improvedprocedure.
Severalacidandenzyme-basedmethodsforthehydrolysisofβ-glucanwerecomparedandthebestoptionwasthemethodusingH2SO4.Formostsamples,similarβ-glucanvalueswereobtainedwithboththeoptimizedHClandH2SO4procedures.However,inthecaseofcertainsamples,specificallyGanodermalucidumandPoriacocus,theH2SO4procedureresultedinsignificantlyhighervalues.
McCleary,B.V.&Draga,A.(2016).Measurementofβ-GlucaninMushroomsandMycelialProducts.JournalofAOACInternational,Vol.99,No.2.
Thekitcomponentshavenotbeenalteredfrompreviouslots.
Measurementofβ-GlucaninMushroomsandMycelialProducts.
McCleary,B.V.&Draga,A.(2016).JournalofAOACInternational,99(2),364-373.
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Arobustandreliablemethodhasbeendevelopedforthemeasurementofβ-glucaninmushroomandmycelialproducts.Totalglucan(plusfreeglucoseandglucosefromsucrose)wasmeasuredusingcontrolledacidhydrolysiswithH2SO4andtheglucosereleasedspecificallywasmeasuredusingglucoseoxidase/peroxidasereagent.α-Glucan(starch/glycogen)plusfreeglucoseandglucosefromsucrosewerespecificallymeasuredafterhydrolysisofstarch/glycogentoglucosewithglucoamylaseandsucrosetoglucoseplusfructosewithinvertaseandtheglucosespecificallymeasuredwithGOPODreagent.β-Glucanwasdeterminedbythedifference.Severalacidandenzyme-basedmethodsforthehydrolysisoftheβ-glucanwerecompared,andthebestoptionwasthemethodusingH2SO4.Formostsamples,similarβ-glucanvalueswereobtainedwithboththeoptimizedHClandH2SO4procedures.However,inthecaseofcertainsamples,specificallyGanodermalucidumandPoriacocus,theH2SO4procedureresultedinsignificantlyhighervalues.Hydrolysiswith2Ntrifluoroaceticacidat120°Cwasfoundtobemuchlesseffectivethaneitheroftheothertwoacidsevaluated.Assaysbasedtotallyonenzymatichydrolysis,ingeneral,yieldedmuchlowervaluesthanthoseobtainedwiththeH2SO4procedure.
Screeningofbeta-glucancontentsincommerciallycultivatedandwildgrowingmushrooms.
Sari,M.,Prange,A.,Lelley,J.I.&HambitzerR.(2016).FoodChemistry,216,45-51.
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Mushroomshaveuniquesensorypropertiesandnutritionalvaluesaswellashealthbenefitsduetotheirbioactivecompounds,especiallybeta-glucans.Well-knownedIBLeandmedicinalmushroomspeciesaswellasuncommonorunknownspeciesrepresentinginterestingsourcesofbioactivebeta-glucanshavebeenwidelystudied.Commerciallycultivatedandwildgrowingmushroomswereanalysedfortheirbeta-glucancontents.Enzymaticdeterminationsofallglucans,alpha-glucansandbeta-glucansin39mushroomsspecieswereperformed,leADIngtoveryremarkableresults.Manywildgrowingspeciespresenthighbeta-glucancontents,especiallyBracketfungi.Thewell-knowncultivatedspeciesAgaricusbisporus,LentinulaedodesandCantharelluscibariusaswellasmostscreenedwildgrowingspeciesshowhigherglucancontentsintheirstipesthancaps.
Characterizationofcross-flowultrafiltrationfractionsfromMaitakeMedicinalMushroom,Grifolafrondosa(Agaricomycetes),RevealsDistinctCytotoxicityinTumorCells.
Sari,M.,Hambitzer,R.,Lelley,J.I.,Toepler,K.,Teusch,N.,Nickisch-Hartfiel,A.(2016).InternationalJournalofMedicinalMushrooms,18(8),671-680.
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β-glucansfromBasidiomyceteslikeGrifolafrondosa(themaitakemushroom)arewellknownfortheirhealthbenefits.PolysaccharidepreparationsfrommedicinalmushroomssuchasG.frondosahavebeensuccessfullytestedinavastnumberofstudies.Manymushroomextractshavebeendevelopedandtodayaremerchandizedforusemedicinallyandcommercially.Studiescouldshowthat,inparticular,chemicalstructuralfeaturessuchasthemolecularsizeofβ-glucanssignificantlyinfluencetheirbioactivity.Thusitishighlyimportanttoexplorethecompositionandstructuralpropertiesofβ-glucansextractedfrommedicinalmushroomsandtheireffectsonhumantumorcellviability.Ourstudyfocusesonthemolecularweightcutoffdistributionofβ-glucansinhotwater-basedextractsfrommaitakemushrooms.Cross-flowultrafiltrationwasappliedtoobtain5fractionsofdifferentmolecularsize.β-glucancontentwasquantifiedusinganenzyme-basedtestkit,specializedto1,3-1,6-β-glucans.Hereweshowthatonlysmallamountsofβ-glucanswithahighmolecularweight(>100kDa)couldbedetectedfromanaqueousextractofG.frondosa.Themaincompoundsencompasssubstanceswithalowmolecularweight(<5 kda),="" composing="" about="" 35%="" of="" the="" whole="" extract.="" in="" addition,="" tumor="" cell="" viability="" studies="" demonstrate="" significant="" cytotoxic="" potential="" in="" 2="" different="" solid="" cancer="" cell="" types="" for="" the="" fraction="" with="" a="" high="" molecular="" weight="" (="">100kDa)andfor1fractionwithalowmolecularweight(5-10kDa).Insummary,ourexperimentsprovethatcross-flowultrafiltrationservesasaquickandeasymethodfordividingcrudeaqueousmushroomextractsintodifferentmolecular-weightfractionsthatinhibittumorcellviABIlityinvitro.
MushroomsofgenusPleurotusasasourceofdietaryfibresandglucansforfoodsupplements.
Synytsya,A.,MíčKoVá,K.,Jablonský,I.,Sluková,M.&Čopíková,J.(2008).CzechJournalofFoodSciences,26(6),441-446.
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Fruitbodies(separatelypileiandstems)ofmushroomsPleurotusostreatus(fourstrains)andPleurotuseryngiiwerecharacterisedasasourceofpolysaccharides.ThecontentsofglucansanddietaryfibresweredeterminedwithusingtherespectiveMegazymeenzymatickits.Enzymaticanalysisofthefruitbodiesconfirmedsignificantdifferencesinthecontentsofthesecomponentsamongthespeciesandstrains.Thestemscontainedmoreinsolubledietaryfibresthanthepileiinallthecasesandmoreβ-glucansinmostcases.However,relativelyhighcontentsofβ-glucan(20–50%ofdrymatter)couldbearesultofincompleteenzymatichydrolysisofinsolubleα-1,3-glucans.Nevertheless,lowfoodqualitystemsofmushroomsPleurotussp.couldbeavaluablesourceofcellwallglucansforthepreparationoffoodsupplements.
GlucansfromfruitbodiesofcultivatedmushroomsPleurotusostreatusandPleurotuseryngii:Structureandpotentialprebioticactivity.
Synytsya,A.,Míčková,K.,Synytsya,A.,Jablonský,I.,Spěváček,J.,Erban,V.,Kováříková,E.&Čopíková,J.(2009).CarbohydratePolymers,76(4),548-556.
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Cultivatedoystermushrooms(genusPleurotus)areinterestingasasourceofBIOLOGicallyactiveglucans.Partially,β-glucanfromPleurotussp.(pleuran)hasbeenusedasfoodsupplementsduetoitsimmunosuppressiveactivity.Likeotherdietaryfibrecomponents,oystermushroompolysaccharidescanstimulatethegrowthofcolonmicroorganisms(probiotics),i.e.actasprebiotics.SpecificglucanswereisolatedfromstemsofPleurotusostreatusandPleurotuseryngiibysubsequentboilingwaterandalkaliextraction.Obtainedwatersoluble(L1),alkalisoluble(L2)andinsoluble(S)fractionswerecharacterisedbyvariousanalyticalmethods.Spectroscopicanalysisdetectedglucansinallthefractions:branched1,3-1,6-β-D-glucanpredominatedinL1andS,whilelinear1,3-α-D-glucaninL2.FractionsL1alsocontainedmarkedamountofproteinspartiallyincomplexwithglucans;proteincontentinL2wasinsignificant.EffectivedeproteinisationofL1andseparationofα-andβ-glucansinL2wasachievedbythetreatmentwithphenolicreagent.SmallamountofchitinwasfoundinSasacomponentofcellwallchitin–glucancomplex.PotentialprebioticactivityofextractsL1andL2wastestingusingnineprobioticstrainsofLactobacillus,BifidobacteriumandEnterococcus.Theseprobioticsshoweddifferentgrowthcharacteristicsdependentlyonusedextractandstrainspecificityduetothepresenceofstructurallydiversecompounds.TheextractsL1andL2canbeappliedtosynbioticconstructiononlyforcarefullyselectedprobioticstrains.ThisexploitationoffruitbodyextractsextendstheuseofmushroomsP.ostreatusandP.eryngiiforhumanhealth.
HighCellDensityFermentationofSaccharomycescerevisiaeJUL3inFed-batchCulturefortheProductionofβ-Glucan.
Kim,Y.H.,Kang,S.W.,Lee,J.H.,Chang,H.L.,Yun,C.W.,Paik,H.D.,Kang,C.W.&Kim,S.W.(2007).JournalofIndustrialandEngineeringChemistry-Seoul-,13(1),153-158.
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β-Glucanisacellwallcomponentthatisoneofthemostplentifulcellpolysaccharides.Moreover,ithasbeenfoundtohaveseveralbeneficialeffectsontheimmunesystem.Inyeast,β-glucanismainlycontainedintheyeastcellwall,andthusitisimportanttoproducehighlevelsofcellmassforthemassproductionofyeastβ-glucan.Responsesurfacemethodology(RSM)offersapotentialmeansofoptimizingprocessfactorsandmediumcomponents;ithasbeenusedtoestimatetheeffectsofmediumcomponentsoncellmassproduction.Inthepresentstudy,theoptimalconcentrationsofmolassesandcornsteepliquor(CSL)inthemediumweredeterminedtobe6.4%(v/v)and17%(v/v).Thecellmasspredictedbystatisticalanalysiswas9.76g/Lafter20hofcultivation.Ina2.5-Lstirredtankreactor(STR),thecellmassproducedinabatchculturewas36.5∼39.3g/L.Themaximumcellmassinthefed-batchculturesofSaccharomycescerevisiaeJUL3was95.7g/Lusing50%molassessolutionandafeedrateof10mL/h.Thecellmassobtainedinthefed-batchculturewas2.4-foldhigherthanthatobtainedinthebatchculture.
Developmentofaloefermentationproductsandimprovementsofgastrointestinalfunctioninvitro.
Cho,J.H.,Baik,S.O.,Kim,C.S.,Kim,H.H.,Jung,E.J.,Kim,H.K.&Kim,B.K.(2012).FoodScienceandBiotechnology,21(1),35-42.
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ThreealoefermentationproductsfromGanodermalucidum(AG),Hericiumerinaceum(AH),andPhellinuslinteus(AP)wereobtainedbyfermentingmushroommyceliausingaloeasasubstrate.WhenAG,AH,andAPwereaddedtosterilizedaloeandfermentedfor5days,thecolorofthealoefermentationproductchangedfrompinktobeige,whichisaloe’snaturalcolor,throughthefermentationtime.ThepHofthealoefermentationproductsranged4.32–4.36shortlyafterinoculationandthen4.62–4.68duringthe5daysoffermentation.pHincreasedby7%duringthetotalfermentationtime.Thesolidcontenthadincreased1.28–1.40times.ThecontentsofaloinAandBincreasedwithfermentationtime.β-Glucancontentdecreasedwithfermentationtime.Theureaseinhibitionactivity(%)wereremarkableinAG-496.70,AH-492.30,andAP-466.40%,indicatingtheseproductshadgrowthinhibitioneffectsagainstHelicobacterpylori.Moreover,AGandAHweremosteffectiveasanti-H.pyloritreatments.
Responsesurfaceoptimizationofβ-glucanextractionfromcauliflowermushrooms(Sparassiscrispa).
Bae,I.Y.,Kim,K.J.,Lee,S.&Lee,H.G.(2012).FoodScienceandBiotechnology,21(4),1031-1035.
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Theextractionproceduresforβ-glucansfromcauliflowermushrooms(Sparassiscrispa)wereoptimizedbyresponsesurfacemethodology.Experimentaldesignwasusedtoinvestigatetheeffectof3extractionparameters(pH,extractiontime,andratioofwatertorawmaterial)onβ-glucancontent.Theparameterrangesinvestigatedwere6–10forextractionpH(X1),5-15hforextractiontime(X2),and10–30forwatertorawmaterialratio(X3).Theexperimentalresultswereingoodagreementwithapolynomialregressionmodelbyamultipleregressionanalysis(R2=0.95,p=0.0074)forβ-glucancontentextractedfromcauliflowermushrooms.Theoptimalconditionsforβ-glucanextractionfromcauliflowermushroomsweredeterminedasextractionpHof6.05,extractiontimeof8h55min,andratioofwatertorawmaterialof19.74,showing60.76%ofthepredictedcontentofβ-glucan.
Sparassiscrispasuppressesmastcell-mediatedallergicinflammation:Roleofcalcium,mitogen-activatedproteinkinaseandnuclearfactor-κB.
Kim,H.H.,Lee,S.,Singh,T.S.,Choi,J.K.,Shin,T.Y.&Kim,S.H.(2012).InternationalJournalofMolecularMedicine,30(2),344-350(7).
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Allergicinflammatorydiseasesuchasfoodallergy,asthmaandatopicdermatitisareincreasingworldwide.Inthisstudy,weinvestigatedtheeffectofwaterextractofSparassiscrispa(WESC)Fr.(Aphyllophoromycetideae)onmastcell-mediatedallergicinflammationandthepossiblemechanismsofaction.WESCinhibitedcompound48/80-inducedsystemicanaphylaxisandserumhistaminereleaseinmice.WESCdecreasedimmunoglobulinE(IgE)-mediatedpassivecutaneousanaphylaxis.Additionally,WESCreducedhistaminereleaseandintracellularcalciuminhumanmastcellsactivatedbyphorbol12-myristate13-acetate(PMA)andcalciumionophoreA23187.WESCdecreasedPMAandA23187-stimulatedexpressionofpro-inflammatorycytokines,suchastumornecrosisfactor(TNF)-α,inlerleukin(IL)-6andIL-1β.TheinhibitoryeffectofWESConpro-inflammatorycytokineswasnuclearfactor-κB,extracellularsignal-regulatedkinaseandp38mitogen-activatedproteinkinase-dependent.OurresultssuggestpotentialtherapeuticapplicationofWESCinallergicinflammatorydiseases.
AntioxidativeandimmunomodulatingactivitiesofpolysaccharideextractsofthemedicinalmushroomsAgaricusbisporus,Agaricusbrasiliensis,GanodermalucidumandPhellinuslinteus.
Kozarski,M.,Klaus,A.,Niksic,M.,Jakovljevic,D.,Helsper,J.P.F.G.&VanGriensven,L.J.L.D.(2011).Foodchemistry,129(4),1667-1675.
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Partiallypurifiedpolysaccharideswereobtainedfromfourmedicinalmushroomspecies,Agaricusbisporus,Agaricusbrasiliensis,PhellinuslinteusandGanodermalucidumbyhotwaterextraction,followedbyethanolprecipitation.Thefoursamplescontainedvaryingamountsofbothα-andβ-glucansasdeterminedbyFT-IRandbyquantitativeestimationafterpriorpartialhydrolysis(Megazymeβ-glucanassaykit).EC50valuesoftheDPPHscavengingactivityofthepolysaccharidesfromG.lucidumsporesandP.linteusfruitingbodieswerefoundtobeparticularlylow,i.e.EC50<0.1=""mg/ml.=""for="">A.brasiliensisandA.bisporus,EC50valueswere0.27and2.0mg/ml.EC50valuesoftheantioxidantactivitywere7.07mg/mlforG.lucidum,13.25mg/mlforA.brasiliensisand>20mg/mlforA.bisporuspolysaccharide,respectively.EC50valuesofthechelatingactivityofferrousionsrangedfrom0.59mg/mlforG.lucidumto7.80mg/mlforA.bisporus.TheEC50valuesoftheextractsinthereducingpowerassayrangedfrom0.47to14.83mg/ml.AcorrelationwasfoundbetweenEC50valuesofthechelatingandreducingpowerabilitiesandtheamountoftotalglucanscontentintheextracts.InvitromeasurementsofimmunomodulatorycapacityofpolysaccharideextractsshowedthatA.bisporus,A.brasiliensisfruitingbodiesandG.lucidumsporesextractsexpressanimmunostimulatingeffectonactivatedhumanPBMCsandinducesynthesisofIFN-γ.ThepolysaccharideextractofP.linteusfruitingbodiesshowedanimmunosuppressiveeffect.
Newmethoddevelopmentfornanoparticleextractionofwater-solubleβ-(1→3)-D-glucanfromediblemushrooms,SparassiscrispaandPhellinuslinteus.
Park,H.G.,Shim,Y.Y.,Choi,S.O.&Park,W.M.(2009).JournalofAgriculturalandFoodChemistry,57(6),2147-2154.
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SparassiscrispaandPhellinuslinteusareedible/medicinalmushroomsthathaveremarkablyhighcontentsofβ-(1→3)-D-glucan,whichactsasabiologicalresponsemodifier,butdifficultyincultivatingthefruitingbodiesandextractionofβ-D-glucanhaverestricteddetailedstudies.Therefore,anovelprocessfornanoparticleextractionofSparan,theβ-D-glucanfromSparassiscrispa,andPhellin,theβ-D-glucanfromPhellinuslinteus,hasbeeninvestigatedusinginsolubletungstencarbideasamodelfornanoknifetechnology.ThisisthefirstreportshowingthatthenanoknifemethodresultsinhighyieldsofSparan(70.2%)andPhellin(65.2%)withanaverageparticlesizeof150and390nm,respectively.TheextractedSparanwithβ-(1→3)linkagesshowedaremarkablyhighwatersolubilityof90%evenafter10minofincubationatroomtemperature.Therefore,itislikelythatthisnanoknifemethodcouldbeusedtoproduceβ-D-glucanforfood,cosmetic,andpharmaceuticalindustries.
OptimizationofbiomassproductionwithenhancedglucananddietaryfibrescontentbyPleurotusostreatusATHUM4438undersubmergedculture.
Papaspyridi,L.M.,Katapodis,P.,Gonou-Zagou,Z.,Kapsanaki-Gotsi,E.&Christakopoulos,P.(2010).BiochemicalEngineeringJournal,50(3),131-138.
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ThisworkwasaimedatoptimizingbiomassproductionbytheediblebasidiomycetePleurotusostreatusATHUM4438inasubmergedprocesswithenhancedglucananddietaryfibrescontent.β-GlucanfromPleurotussp.(pleuran)hasbeenusedasfoodsupplementsduetoitsimmunosuppressiveactivity.Likeotherdietaryfibrecomponents,oystermushroompolysaccharidescanstimulatethegrowthofcolonmicroorganisms(probiotics),i.e.actasprebiotics.WeusedtheFFMicroPlateforsubstrateutilizationandgrowthmonitoring.Thepatternofsubstratecatabolismformsasubstrateassimilationfingerprintwhichisusefulinselectingmediacomponentsformediaoptimizationofmaximumbiomassproduction.Differentcarbonsources(95)wereusedandthen8ofthemweretestedinshakeflaskcultures.Theeffectofvariousorganicandcomplexnitrogensourcesonbiomassproductionwasalsoexaminedandresponsesurfacemethodologybasedoncentralcompositedesignwasappliedtoexploretheoptimalmediumcomposition.Whentheoptimizedculturemediumwastestedina20-Lstirredtankbioreactor,using57gL-1xyloseand37gL-1cornsteepliquor,highyields(39.2gL-1)ofdrybiomasswasobtained.Theyieldcoefficientsfortotalglucananddietaryfibresonmycelialbiomassformedwere140±4and625±9mgg-1myceliumdryweight,respectively.
Solubleβ-1,3/1,6-glucaninseaweedfromthesouthernhemisphereanditsimmunomodulatoryeffect.
Bobadilla,F.,Rodriguez-Tirado,C.,Imarai,M.,Galotto,M.J.&Andersson,R.(2013).Carbohydratepolymers,92(1),241-248.
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Fivetypesofmacroalgaefromthesouthernhemispherewereanalysedforthepresenceofβ-1,3/1,6-glucananditsimmunostimulantproperties.Wewereabletoextractsolubleβ-1,3/1,6-D-glucanfromDurvillaeaantarctica(Chamisso)Hariot(DA).Themorphologyofthebrownalgaeinfluencedextraction,andthehighestpercentageofβ-glucanwasfoundinthefronds.Thecontentofβ-glucaninthestipesandholdfastwasonaverage33%and<5%, respectively,="" of="" that="" in="" the="" fronds.="" a="" simple="" laboratory="" extraction="" process="" was="" developed.="" a="" highly="" pure="" water-soluble="" polysaccharide,="" mainly="" composed="" of="" glucose="" residues,="" was="" obtained="" with="" a="" dominant="" average="" molecular="" weight="" of="" 6.9="" kda.="" nmr="" spectroscopy="" confirmed="" the="" polysaccharide="" structure="" to="" be="" of="" β-1,3/1,6-glucan="" type,="" comprising="" a="" β-1,3-glucan="" backbone="" and="" 21%="" degree="" of="" branching="" of="" β-1,6-glucan="" side="" chains.="" mouse="" cells="" were="" exposed="" to="" four="" da="" extract="" concentrations="" in="" water="" (50,="" 100,="" 250="" and="" 500="" μg/ml)="" and="" no="" adverse="" effects="" on="" survival="" were="" noted.="" remarkably,="" the="" β-glucan="" induced="" a="" 16.9%="" increase="" in="" activated="" cd19+="" b="" lymphocytes="" compared="" with="" the="" control="" sample.="" the="" optimal="" concentration="" for="" maximum="" activity="" was="" 100="" μg="" da="" extract/ml.="">
ColourimetricmethodforthedeterminationofYeastand
Mushroomβ-Glucaninyeast,mushroom,foodstuffsand
othermaterials
Principle:
(12MH2SO4,0°C,2h)
(1)1,3:1,6-β-Glucan+1,3-β-glucan+α-glucan+H2O→
solubleglucan
(1MH2SO4,100°C,2h)
(2)Solubleglucan+H2O→D-glucose+laminarisaccharides(trace)
(exo-1,3-β-glucanase+β-glucosidase)
(3)Laminarisaccharides+H2O→D-glucose
(glucoseoxidase)
(4)D-Glucose+H2O+O2→D-gluconate+H2O2
(peroxidase)
(5)2H2O2+p-hydroxybenzoicacid+4-aminoantipyrine→
quinoneimine+4H2O
(amyloglucosidase)
(6)α-Glucan+H2O → D-glucose
Kitsize: 100assays
Method: Spectrophotometricat510nm
Totalassaytime: ~100min
Detectionlimit: 1-100%ofsampleweight
Applicationexamples:
Yeastpreparations,mushroompreparationsandothermaterials
Methodrecognition: Novelmethod
Advantages
- Verycosteffective
- Allreagentsstablefor>12monthsafterpreparation
- Onlyenzymatickitavailable
- Simpleformat
- Mega-Calc™softwaretoolisavailablefromourwebsiteforhassle-freerawdataprocessing
- Standardincluded
品牌介绍
Megazyme品牌产品简介
来源：作者：人气：2149发表时间：2016-05-19 10:59:00【大 中 小】
Megazyme是一家全球性公司，专注于开发和提供用于饮料、谷物、乳制品、食品、饲料、发酵、生物燃料和葡萄酒产业用的分析试剂、酶和检测试剂盒。Megazyme的许多检测试剂盒产品已经为众多官方科学协会（包括AOAC, AACC , RACI, EBC和ICC等），经过严格的审核，批准认证为官方标准方法，确保以准确、可靠、定量和易于使用的测试方法，满足客户的质量诉求。
Megazyme的主要产品线包括：
◆ 检测试剂盒
◆ 酶
◆ 酶底物
◆ 碳水化合物
◆ 化学品/仪器
官网地址：http://www.megazyme.com
检测试剂盒特色产品：
货号
中文品名
用途
K-ACETAF
乙酸[AF法]检测试剂盒
酶法定量分析乙酸最广泛使用的方法
K-ACHDF
可吸收糖/膳食纤维检测试剂盒
酒精沉淀法测定膳食纤维
K-AMIAR
氨快速检测试剂盒
用于包括葡萄汁、葡萄酒以及其它食品饮料样品中氨含量的快速检测分析。
K-AMYL
直链淀粉/支链淀粉检测试剂盒
谷物淀粉和而粉中直链淀粉/支链淀粉比例和含量检测
K-ARAB
阿拉伯聚糖检测试剂盒
果汁浓缩液中阿拉伯聚糖的检测
K-ASNAM
L-天冬酰胺/L-谷氨酰胺和氨快速检测试剂盒
用于食品工业中丙烯酰胺前体、细胞培养基、以及上清液组分中、L-天冬酰胺，谷氨酰胺和氨的检测分析
K-ASPTM
阿斯巴甜检测试剂盒
专业用于测定饮料和食品中阿斯巴甜含量，操作简单
K-BETA3
β-淀粉酶检测试剂盒
适用于麦芽粉中β-淀粉酶的测定
K-BGLU
混合键β-葡聚糖检测试剂盒
测定谷物、荞麦粉、麦汁、啤酒及其它食品中混合键β-葡聚糖(1,3:1,4-β-D-葡聚糖)的含量
K-CERA
α-淀粉酶检测试剂盒
谷物和发酵液（真菌和细菌）中α-淀粉酶的分析测定
K-CITR
柠檬酸检测试剂盒
快速、可靠地检测食品、饮料和其它物料中柠檬酸（柠檬酸盐）含量
K-DLATE
乳酸快速检测试剂盒
快速、特异性检测饮料、肉类、奶制品和其它食品中L-乳酸和D-乳酸(乳酸盐）含量
K-EBHLG
酵母β-葡聚糖酶检测试剂盒
用于测量和分析酵母中1,3:1,6?-β-葡聚糖，也可以检测1,3-葡聚糖
K-ETSULPH
总亚硫酸检测试剂盒
测定葡萄酒、饮料、食品和其他物料中总亚硫酸含量（按二氧化硫计）的一种简单，高效，可靠的酶法检测方法
K-FRGLMQ
D-果糖/D-葡萄糖[MegaQuant法]检测试剂盒
适用于使用megaquant?色度计（505nm下）测定葡萄、葡萄汁和葡萄酒中D-果糖和D-葡萄糖的含量。
K-FRUC
果聚糖检测试剂盒
含有淀粉、蔗糖和其他糖类的植物提取物和食品中果聚糖的含量测定。
K-FRUGL
D-果糖/D-葡萄糖检测试剂盒
对植物和食品中果糖或葡萄糖含量的酶法紫外分光测定。
K-GALM
半乳甘露聚糖检测试剂盒
食品和植物产品中半乳甘露聚糖的含量检测
K-GLUC
D-葡萄糖[GOPOD]检测试剂盒
谷物提取物中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUHK
D-葡萄糖[HK]检测试剂盒
植物和食品中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUM
葡甘聚糖检测试剂盒
植物和食品中葡甘聚糖的含量测定。
K-INTDF
总膳食纤维检测试剂盒
总膳食纤维特定检测和分析
K-LACGAR
乳糖/D-半乳糖快速检测试剂盒
用于快速检测食品和植物产品中乳糖、D-半乳糖和L-阿拉伯糖
K-LACSU
乳糖/蔗糖/D-葡萄糖检测试剂盒
混合面粉和其它物料中蔗糖、乳糖和D-葡萄糖的测定
K-LACTUL
乳果糖检测试剂盒
特异性、快速和灵敏测量奶基样品中乳果糖含量
K-MANGL
D-甘露糖/D-果糖/D-葡萄糖检测试剂盒
适合测定植物产品和多糖酸性水解产物中D-甘露糖含量
K-MASUG
麦芽糖/蔗糖/D-葡萄糖检测试剂盒
在植物和食品中麦芽糖，蔗糖和葡萄糖的含量检测
K-PECID
胶质识别检测试剂盒
食品配料中果胶的鉴别
K-PHYT
植酸(总磷)检测试剂盒
食品和饲料样品植酸/总磷含量测量的简便方法。不需要通过阴离子交换色谱对植酸纯化，适合于大量样本分析
K-PYRUV
丙酮酸检测试剂盒
在啤酒、葡萄酒、果汁、食品和体液中丙酮酸分析
K-RAFGA
棉子糖/D-半乳糖检测试剂盒
快速测量植物材料和食品中棉子糖和半乳糖含量
K-RAFGL
棉子糖/蔗糖/D-半乳糖检测试剂盒
分析种子和种子粉中D-葡萄糖、蔗糖、棉子糖、水苏糖和毛蕊花糖含量。通过将棉子糖、水苏糖和毛蕊花糖酶解D-葡萄糖、D-果糖和半乳糖，从而测定葡萄糖含量来确定
K-SDAM
淀粉损伤检测试剂盒
谷物面粉中淀粉损伤的检测和分析
K-SUCGL
蔗糖/D-葡萄糖检测试剂盒
饮料、果汁、蜂蜜和食品中蔗糖和葡萄糖的分析
K-SUFRG
蔗糖/D-果糖/D-葡萄糖检测试剂盒
适用于植物和食品中蔗糖、D-葡萄糖和D-果糖的测定
K-TDFR
总膳食纤维检测试剂盒
总膳食纤维检测
K-TREH
海藻糖检测试剂盒
快速、可靠地检测食品、饮料和其它物料中海藻糖含量
K-URAMR
尿素/氨快速检测试剂盒
适用于水、饮料、乳制品和食品中尿素和氨的快速测定
K-URONIC
D-葡萄糖醛酸/D-半乳糖醛酸检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中六元糖醛酸含量（D-葡萄糖醛酸和D-半乳糖醛酸）
K-XYLOSE
D-木糖检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中D-木糖含量
K-YBGL
Beta葡聚糖[酵母和蘑菇]检测试剂盒
检测酵母和蘑菇制品中1,3:1,6-beta-葡聚糖和α-葡聚糖含量
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