Megazyme/乙醇分析试剂盒/K-ETOH/60分析（手动）/600分析（微孔板）/600分析（自动分析仪）
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K-ETOH
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Megazyme INC
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¥2560.00
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1536.00
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其它检测试剂盒
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Other_kits
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3392242852
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4000-520-616
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info@ebiomall.com
商品介绍
TheEthanoltestkitisa simple,reliableandaccuratemethodforthemeasurementandanalysisofethanolinbeveragesandfoodstuffs.
ExtendedcofactorsstABIlity.Dissolvedcofactorsstablefor>1yearat4oC.
Suitableformanual,auto-analyserandmicroplateformats.
Grapeandwineanalysis:Oenologiststoexploitadvancedtestkits.
Charnock,S.C.&McCleary,B.V.(2005).RevuedesEnology,117,1-5.
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Itiswithoutdoubtthattestingplaysapivotalrolethroughoutthewholeofthevinificationprocess.ToproducethebestpossIBLequalitywineandtominimiseprocessproblemssuchas“stuck”fermentationortroublesomeinfections,itisnowrecognisedthatifpossibletestingshouldbeginpriortoharvestingofthegrapesandcontinuethroughtobottling.TrADItionalmethodsofwineanalysisareoftenexpensive,timeconsuming,requireeitherelaborateequipmentorspecialistexpertiseandfrequentlylackaccuracy.However,enzymaticbio-analysisenablestheaccuratemeasurementofthevastmajorityofanalytesofinteresttothewinemaker,usingjustonepieceofapparatus,thespectrophotometer(seepreviousissueNo.116foradetailedtechnicalreview).Grapejuiceandwineareamenabletoenzymatictestingasbeingliquidstheyarehomogenous,easytomanipulate,andcangenerallybeanalysedwithoutanysamplepreparation.
Megazyme“advanced”winetestkitsgeneralcharacteristicsandvalidation.
Charnock,S.J.,McCleary,B.V.,Daverede,C.&Gallant,P.(2006).ReveuedesOenologues,120,1-5.
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ManyoftheenzymatictestkitsareofficialmethodsofprestigiousorganisationssuchastheAssociationofOfficialAnalyticalChemicals(AOAC)andtheAmericanAssociationofCerealChemists(AACC)inresponsetotheinterestfromoenologists.Megazymedecidedtouseitslonghistoryofenzymaticbio-analysistomakeasignificantcontributiontothewineindustry,bythedevelopmentofarangeofadvancedenzymatictestkits.Thistaskhasnowbeensuccessfullycompletedthroughthestrategicandcomprehensiveprocessofidentifyinglimitationsofexistingenzymaticbio-analysistestkitswheretheyoccurred,andthenusingadvancedtechniques,suchasmolecularBIOLOGy(photo1),torapidlyovercomethem.Noveltestkitshavealsobeendevelopedforanalytesofemerginginteresttotheoenologist,suchasyeastavailablenitrogen(YAN;seepages2-3ofissue117article),orwherepreviouslyenzymesweresimplyeithernotavailable,orweretooexpensivetoemploy,suchasforD-mannitolanalysis.
LactosefermentationbyKombucha–aprocesstoobtainnewmilk–basedbeverages.
Iličić,M.,Kanurić,K.,Milanović,S.,Lončar,E.,Djurić,M.&Malbaša,R.(2012).RomanianBiotechnologicalLetters,17(1),7013-7021.
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Thispaperfocusesonfermentationoflactosefromamodelsystem(blacktea)andfromtwotypesofmilk(0.9%w/wand2.2%w/woffat)byapplicationofKombucha.QuantitiesoftheappliedKombuchastarterwere10%v/vand15%v/v.Allfermentationswereperformedat42°C.TheprocesstoachieveadesirablepH=4.5wasslowerinthemodelsystem(16h)thaninmilks(9-10h).Regardingstarterquantity,10%v/vprovedtheoptimal.Regardingtypesofmilk,higherfatcontentguaranteesshorterfermentationandhigheryieldofmetabolites.Utilizationoflactosewasfoundatalevelof≈20%and≈30%inmilkswith0.9%w/wand2.2%w/woffat,respectively.Thiswascorrelatedwithanappearanceofintermediatesand/orproducts.Glucoseunderwentfurthertransformationsalmostentirely,whilegalactoseshowedmuchlowerreactivity.Seventotwelvetimeshighercontentsoflacticacidwerefoundcomparedtoaceticacid.Milk-basedbeveragefromthereducedfatsample,inoculatedwith10%v/vofKombuchastarter,hasthebestphysicalcharacteristics(syneresisandwaterholdingcapacity).Italsodevelopedagoodtexture(especiallycohesivenessandindexofviscosity).Milklactosefermentationwasaprocessthatcouldhavebeenusedforobtainingnewmilk-basedproducts.
Changesinthevolatilecompoundproductionoffermentationsmadefrommustswithincreasinggrapecontent.
Keyzers,R.A.&Boss,P.K.(2010).JournalofAgriculturalandFoodChemistry,58(2),1153-1164.
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Wineisacomplexconsumerproductproducedpredominatelybytheactionofyeastupongrapejuice.Modelmustsystemshaveproventobeidealforstudiesintotheeffectsoffermentationconditionsontheproductionofcertainwinevolatiles.Toclarifythecontributionofgrapejuicetotheproductionofwinevolatiles,wehaveemployedamodelmustsystemspikedwithincreasingamountsofgrapejuice(RieslingorCabernetSauvignon).TheresultingfermentedwineswereanalyzedbySPME-GC-MSandthedataobtainedgroupedusingANOVAandclusteranalysestorevealthosecompoundsthatvariedinconcentrationwithreproducibletrendsrelativetojuiceconcentration.Suchgroupinghighlightsthosecompoundsthataregrape-dependentorforwhichproductionismodulatedbygrapecomposition.Insomecases,increasingtheproportionofgrapejuiceinthefermentationsstimulatedtheproductionofcertainesterstolevelsbetween2-and140-foldhigherthanthoseseeninfermentationsmadewithmodelgrapejuicemediaalone.Theidentificationofthegrapecomponentsresponsiblefortheincreasedproductionofthesewinevolatileswillhaveimplicationsfortheimpactofgrapeproductionandenologyonwineflavorandaroma.
MetabolicengineeringofSaccharomycescerevisiaetominimizetheproductionofethylcarbamateinwine.
Coulon,J.,Husnik,J.I.,Inglis,D.L.,vanderMerwe,G.K.,Lonvaud,A.,Erasmus,D.J.&vanVuuren,H.J.J.(2006).AmericanJournalofEnologyandViticulture,57(2),113-124.
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Saccharomycescerevisiaemetabolizesarginine,oneofthemajoraminoacidsingrapemusts,toornithineandureaduringwinefermentations.WineyeaststrainsofS.cerevisiaedonotfullymetabolizeureaduringgrapemustfermentation.Ureaissecretedbyyeastcellsanditreactsspontaneouslywithethanolinwinetoformethylcarbamate,apotentialcarcinogenicagentforhumans.ThelackofureacatabolismbyyeastinwinemaybeascribedtothetranscriptionalrepressionoftheDUR1,2genebygoodnitrogensourcespresentinthegrapemust.WeexpressedtheDUR1,2geneundercontroloftheS.cerevisiaePGK1promoterandterminatorsignalsandintegratedthisDUR1,2expressioncassette,flankedbyura3sequences,intotheURA3-locusoftheindustrialwineyeastUCDavis522.InvivoassaysshowedthatthemetabolicallyengineeredindustrialstrainreducedethylcarbamateinChardonnaywineby89.1%.Analysesofthegenotype,phenotype,andtranscriptomerevealedthattheengineeredyeast522EC−issubstantiallyequivalenttotheparental522strain.
Prosomillet(PanicummiliaceumL.)fermentationforfuelethanolproduction.
Rose,D.J.&Santra,D.(2013).IndustrialCropsandProducts,43,602-605.
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Theobjectiveofthisresearchwastodeterminetheconversionefficiencyofprosomillettoethanolcomparedtocorninabench-scaledry-grindprocedure.SevenprosomilletcultivarsandsixadvancedbreedinglinescontainingwaxystarchwerefermentedwithSaccharomycescerevisiaeandethanolproductionwascomparedwithnormalcornand“highlyfermentable”corn.Thehighlyfermentablecornexhibitedthehighestfermentationefficiency(97.0±1.4%).Amongprosomilletlines,thosewiththehighestfermentationefficiencieswere:Huntsman(85.9±0.6%),172-2-9(90.8±0.2%),172-2-13(85.1±2.5%),and182-4-24(84.7±2.1).Waxyprosomilletlinesresultedinhigherfermentationefficienciesthanthenon-waxyprosomilletvarietiescontainingnormalstarch(82.4±5.5%vs.75.5±7.4%,respectively,p=0.01).Prosomilletdistiller"sdriedgrainswithsolubles(DDGS)containedmoreprotein(26.6–33.4%)thantheDDGSfromcorn(17.2–23.4%).Thesedataindicatethatprosomilletexhibitspromiseasafeedstockforethanolproduction,especiallyifbreedingprogramsfocusonselecting“highlyfermentable”linesforadvancement.
BiosynthesisofethanolandhydrogenbyglycerolfermentationusingEscherichiacoli.
Chaudhary,N.,Ngadi,M.O.,Simpson,B.K.&Kassama,L.S.(2011).AdvancesinChemicalEngineeringandScience,1,83-89.
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Productionofhighvalueproductsfromglycerolviaanaerobicfermentationisofutmostimportanceforthebiodieselindustry.ThemicroorganismEscherichiacoli(E.coli)K12wasusedforfermentationofglycerol.Theeffectsofglycerolconcentrationandheadspaceconditionsonthecellgrowth,ethanolandhydrogenproductionwereinvestigated.Afullfactorialexperimentaldesignwith3replicateswasconductedinordertotestthesefactors.Underthethreeheadspaceconditionstested,theincreaseofglycerolconcentrationacceleratedglycerolfermentation.Theyieldsofhydrogenandethanolwerethelowestwhenglycerolconcentrationof10g/Lwasused.Themaximumproductionofhydrogenwasobservedwithaninitialglycerolconcentrationof25g/Latafinalconcentrationofhydrogenwas32.15mmol/L.Thisstudydemonstratedthathydrogenproductionnegativelyaffectscellgrowth.Maximumethanolyieldwasobtainedwithaglycerolconcentrationof10g/Landwasupto0.40g/gglycerolundermembraneconditionheadspace.Statisticaloptimizationshowedthatoptimalconditionsforhydrogenproductionare20g/Linitialglycerolwithinitialspargingofthereactorheadspace.Theoptimalconditionsforethanolproductionare10g/Linitialglycerolwithmembrane.
QuantificationoffullrangeethanolconcentrationsbyusingpHsensor.
Al-Mhanna,N.M.M.&Huebner,H.(2011).InternationalJournalofChemistry,3(1),47-56.
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AdifferentialpHmeasurementdevicewasusedtoachieveoperationconditionsofalcoholdehydrogenasereaction.Optimumoperatingconditionsweretemperatureof30°C,10μlofalcoholdehydrogenaseenzymevolume(withafinalactivityof563.75unitsml-1)per50μlofsample,NAD+concentrationof0.05mMand20mMglycine-pyrophosphatebuffersolutionofpH9.1.Inthismethodarangeofethanolconcentrationsfrom0-99,985%,whichmeans0.000001714-17.14M,wereused.ThemaximumobtainedchangeinpH,deltapH,was(-33)mpH.AcalibrationcurveoflogarithmicvaluesofethanolconcentrationsagainstchangeinpHforstandardethanolsampleswasdone.Sincethiscalibrationcurveisalinearwithacorrelationcoefficient(R)of0.998,thiscalibrationcurvecanbeusedinquantificationofethanolconcentration.Endpointofequilibriumconcentrationsofreactantsandproductsofethanoloxidationreactionwasmeasuredwithinspectrophotometer.Theresultsindicated100secondsofprocesstimeisrequiredtoreachtheendpointforallethanolstandardsamples.ThisrequiredtimewassatisfiedwithresultsofmeasuringchangeinpHwithindifferentialpHanalyzersystem.
ScreeningThermo-andEthanolTolerantBacteriaforEthanolFermentation.
Dung,N.T.P.&Huynh,P.X.(2013).AmericanJournalofMicrobiologicalResearch,1(2),25-31.
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Thethermophilicbacteriareceiveconsiderablyinterestnowadaysbecauseofacurrentchallengeofincreasingglobaltemperature.Particularlyforethanolproduction,thethermo-ethanologenicbacteriapossessadvantagesduetolowercontaminationrisk,costsavinginindustrialscale,andthewiderangeofsugarsutilization.Inthisstudy,13bacterialisolatesobtainedfromthepreviousisolationstudyweretestedfortheirfermentativecapacityandethanoltoleranceathightemperatures.FivebacterialisolatesHM2,M2,MC3,MR1andRDwerefoundtobetolerantupto12%ethanol.OfwhichHM2,M2andMR1couldfermentglucosewellat30,35and40°C,particularlyisolatesHM2andMR1couldperformthefermentativecapacityat45°Candeven50°C.Inthepresenceof12,16,and20%w/vglucose,isolatesHM2,M2,andMR1showedthehighfermentationratebygivinghighgasproduction;however,therateslightlydecreasedinthepresenceof24%w/vglucose.ThefermentativeperformancebythesethreeisolatescouldhappenatdifferentpHlevelsof4.0,5.0and6.0.Thefavourableconditionsofethanolfermentationwerefoundat18.5%glucose,pH5.0,and33°CforisolateHM2andat14%glucose,pH5.5,and40°CforisolateMR1.Theresultsofsequencinganalysisofpartial16SrRNAgeneshowedthatthegenesequencesoftheselectedisolateHM2shared99%similaritywithBacillussubtilis.
Microbiologicalandchemicalpropertiesofkefirmanufacturedbyentrappedmicroorganismsisolatedfromkefirgrains.
Chen,T.H.,Wang,S.Y.,Chen,K.N.,Liu,J.R.&Chen,M.J.(2009).JournalofDairyScience,92(7),3002-3013.
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Inthisstudy,variousyeasts(Kluyveromycesmarxianus,Saccharomycesturicensis,Pichiafermentans)andlacticacidbacteria(Lactobacilluskefiranofaciens,Lactobacilluskefiri,Leuconostocmesenteroides)wereentrappedin2differentmicrospheresusinganentrapmentratioforthestrainsthatwasbasedonthedistributionratiooftheseorganismsinkefirgrains.Thepurposeofthisstudywastodevelopanewtechniquetoproducekefirusingimmobilizedstarterculturesisolatedfromkefirgrains.Anincreaseincellcountswithfermentationcycleswasobservedforboththelacticacidbacteria(LAB)andyeasts,whereasthecellcountsofkefirgrainswereverystableduringcultivation.Scanningelectronmicroscopyshowedthattheshort-chainlactobacilliandlactococcioccupiedthesurfaceoftheLABmicrospheres,whereasthelong-chainlactobacilliwereinsidethemicrospheres.Whentheyeastswereanalyzed,cellsatahighdensitywereentrappedincracksonthesurfaceandwithinthemicrospheres,wheretheyweresurroundedbytheshort-chainlactobacilli.ThedistributionoftheLABandyeastspeciesinkefirproducedfromgrainsandmicrospheresshowedthattherewasnosignificantdifferencebetweenthekefirsproducedbythe2methods;moreover,Leu.mesenteroidesandK.marxianuswerethepredominatingmicroflorainbothtypesofkefir.Therewasnosignificantdifferenceintheethanolandexopolysaccharidecontentsbetweenthe2kefirs,althoughtheaciditywasdifferent.
Taraxeroneenhancesalcoholoxidationviaincreasesofalcoholdehyderogenase(ADH)andacetaldehydedehydrogenase(ALDH)activitiesandgeneexpressions.
Sung,C.K.,Kim,S.M.,Oh,C.J.,Yang,S.A.,Han,B.H.&Mo,E.K.(2012).FoodandChemicalToxicology,50(7),2508-2514.
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Thepresentstudy,taraxerone(D-friedoolean-14-en-3-one)wasisolatedfromSedumsarmentosumwithpurity96.383%,anditsenhancingeffectsonalcoholdehydrogenase(ADH)andacetaldehydedehydrogenase(ALDH)activitiesweredetermined:EC50valueswere512.42±3.12and500.16±3.23μMforADHandALDH,respectively.Inordertoobtainmoreinformationontaraxeronerelatedwiththealcoholmetabolism,40%ethanol(5mL/kgbodyweight)with0.5–1mMoftaraxeronewereadmiNISTeredtomice.Theplasmaalcoholandacetaldehydeconcentrationsoftaraxerone-treatedgroupsweresignificantlyloweredthanthoseofthecontrolgroup(p<0.01):=""approximately=""20–67%=""and=""7–57%=""lowered=""for=""plasma=""alcohol=""and=""acetaldehyde,=""respectively.=""compare=""to=""the=""control=""group,=""the=""adh=""and=""aldh=""expressions=""in=""the=""liver=""tissues=""were=""abruptly=""increased=""in=""the=""taraxerone-treated=""groups=""after=""ethanol=""exposure.=""in=""addition,=""taraxerone=""prevented=""catalase,=""superoxide=""dismutase,=""and=""reduced=""glutathione=""concentrations=""from=""the=""decrease=""induced=""by=""ethanol=""administration=""with=""the=""concentration=""dependent=""manner.="">
FermentationofhighconcentrationsofmaltosebySaccharomycescerevisiaeislimitedbytheCOMPASSmethylationcomplex.
Houghton-Larsen,J.&Brandt,A.AppliedandEnvironmentalMicrobiology,72(11),7176-7182.
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InSaccharomycescerevisiae,genesencodingmaltosepermeasesandmaltasesarelocatedinthetelomericregionsofdifferentchromosomes.TheCOMPASSmethylationcomplex,whichmethylateslysine4onhistoneH3,controlsthesilencingoftelomericregions.YeaststrainsdeletedforSWD1,SWD3,SDC1,SET1,BRE2,orSPP1,encodingcomponentsoftheCOMPASScomplex,fermentedamediumcontaining22%maltosewithnoticeablyhigherattenuationthandidthewildtype,resultinginproductionofupto29%moreethanol.Theleasteffectivestrainwasspp1.AbsenceofCOMPASScomponentshadnoeffectonthefermentationofmediawith20%glucose,20%sucrose,or16%maltose.DeletionofSWD3resultedinlargeramountsofMAL12transcript,encodingmaltase,atthelatestagesoffermentationof22%maltose.Asimilareffectonmaltaseactivityandmaltoseuptakecapabilitywasseen.Thelysine4residueofhistoneH3wastrimethylatedinwild-typecellsatthelatestages,whileonlysmallamountsofthedimethylatedformweredetected.TrimethylationanddimethylationofthisresiduewerenotdetectedinstrainsdeletedforSWD1,SWD3,SET1,BRE2,orSDC1.Trimethylatedlysine4wasapparentonlyattheearlystages(48and96h)offermentationinanspp1strain.ThisworkindicatesthattheCOMPASScomplexrepressestheexpressionofmaltoseutilizationgenesduringthelatestagesoffermentationofahighconcentrationofmaltose.
Single-step,single-organismbioethanolproductionandbioconversionoflignocellulosewastematerialsbyphlebioidfungalspecies.
Mattila,H.,Kuuskeri,J.&Lundell,T.(2017).BioresourceTechnology,225,254-261.
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Ethanolproductionfromnon-pretreatedlignocellulosewascarriedoutinaconsolidatedbioprocesswithwood-decayfungiofphlebioidPolyporales.Ethanolproductionwasattemptedonglucose,sprucewoodsawdustandwastecoreboard.Substantialquantitiesofethanolwereachieved,andisolatePhlebiaradiata0043produced5.9 g/Lofethanolreachingtheyieldof10.4%ethanolfromcoreboardlignocellulosesubstrate.Acidicinitialcultureconditions(pH3)inducedethanolfermentationcomparedtothemoreneutralenvironment.Togetherwithbioethanol,thefungiwereabletoproduceorganicacidssuchasoxalateandfumarate,thusbroadeningtheircapacityandapplicabilityasefficientorganismstobeutilizedforbioconversionofvariouslignocelluloses.Inconclusion,fungiofPhlebiagrowon,convertandsaccharifysolidlignocellulosewastematerialswithoutpre-treatmentsresultinginaccumulationofethanolandorganicacids.Thesefindingswillaidinapplyingfungalbiotechnologyforproductionofbiofuelsandbiocompounds.
Livingcompositesofelectrospunyeastcellsforbioremediationandethanolproduction.
Letnik,I.,Avrahami,R.,Rokem,J.S.,Greiner,A.,Zussman,E.&Greenblatt,C.(2015).Biomacromolecules,16(10),3322-3328.
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Thepreparationofcompositesoflivingfunctionalcellsandpolymersisamajorchallenge.Wehavefabricatedsuch“livingcomposites”bypreparationofpolymericmicrotubesthatentrapyeastcells.Ourapproachwastheprocessofcoaxialelectrospinninginwhichacorecontainingtheyeastwas“spun”withinashellofnonbiodegradablepolymer.WeutilizedtheyeastCandidatropicalis,whichwasisolatedfromolivewaterwaste.Itisparticularlyusefulsinceitdegradesphenolandothernaturalpolyphenols,anditiscapableofaccumulatingethanol.Theelectrospunyeastcellsshowedsignificantactivityofbioremediationofphenolandproducedethanol,and,inaddition,themetabolicprocessesremainedactiveforaprolongedperiod.Comparisonofelectrospuncellstoplanktoniccellsshoweddecreasedcellactivity;however,theolivewaterwasteaftertreatmentbytheyeastwasnolongertoxicforEscherichiacoli,suggestingthatdetoxificationandprolongedviabilityandactivitymayoutweighthereductionofefficiency.
Theroleoflecithinandsolventadditioninethylcellulose-stabilizedheatresistantchocolate.
Stortz,T.A.,Laredo,T.&Marangoni,A.G.(2015).FoodBiophysics,10(3),253-263.
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LargedeformationmechanicaltestingandFourier-transforminfraredspectroscopywereusedtogainfurtherinsightsintothemechanismofheatresistanceinethylcellulose-(EC)stabilizedchocolatepreparedusingthesolventsubstitutionmethod.HereweshowthatthepresenceoflecithinatthesurfaceofsucrosereducedheatresistancebyimpedinginteractionsbetweenECandsucrose.ThesetechniquesalongwithfluorescencemicroscopyalsoshowedthattheEtOHusedinsolventsubstitutionchocolatewasabletoremovetheEtOHsolublelecithinphospholipidsfromthesurfaceofthesucrose.RemovalofthelecithinandtheslightsolubilityofsucroseinEtOHbothhavepositiveimpactsonheatresistance.ItwasalsofoundthatEtOHmayreduceheatresistancebydestabilizingthecaseinmicelleinsamplesmadewithskimmilkpowder.Finally,resultshaveindicatedthatECislikelyabletointeractwiththelactoseinskimmilkpowderandthestarchincocoapowderleadingtogreaterheatresistance.Thesefindingswillbeusefulindevelopingtheidealheatresistantchocolateformula.
Thecompositionofreadilyavailablecarbonsourcesproducedbyfermentationoffishfaecesisaffectedbydietaryprotein:energyratios.
Letelier-Gordo,C.O.,Larsen,B.K.,Dalsgaard,J.&Pedersen,P.B.(2017).AquaculturalEngineering,77,27-32.
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Fishsolidwaste(faeces)producedinrecirculatedaquaculturesystems(RAS)mightbeusedforon-farm,single-sludgedenitrificationiftransformedintosolubleorganiccarbonsubstances.Thecurrentstudyinvestigatedtheeffectoffeedingdietswithincreasingproteintoenergyratios(P:E_15,17,19,21and23 g/MJ)torainbowtrout(Oncorhynchusmykiss)ontheproductionofvolatilefattyacids(VFAs)andethanolduring7 daysfermentationoftheproducedfishfaeces.ThetotalyieldsofVFAsandethanolobtained(expressedaschemicaloxygendemand(COD))rangedbetween0.21–0.24 gCOD/gTCOD,showingnodifferencesbetweentreatments.However,thetypeandquantitiesofindividualVFAsandethanolchangedaccordingtothedietarytreatment.LowerP:Eratiodietsresultedinhigherproductionofbutyricacidandethanol,whereashigherP:Eratiodietsresultedinanincreasedproductionofaceticandvalericacid.Changingthedietcompositionthusaffectsthecompositionofreadilyavailablecarbonthatcanbederivedfromthefaeces.Thiscanbeappliedtoenhanceon-farmsinglesludgedenitrificationandreducetheneedforaddingexternalcarbonsourcessuchase.g.methanol.
EffectsofCudraniaTricUSPidataRootExtract(CTE)onEthanol-InducedHangoverviaModulatingAlcoholMetabolizingEnzymeActivitiesandBloodGasLevelsinRats.
Choi,N.E.,Ro,J.Y.,Lee,J.Y.,Ryu,J.H.&Cho,H.J.(2017).JournaloftheKoreaAcademia-IndustrialcooperationSociety,18(2),218-225.
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Toinvestigatetheanti-hangovereffectsofCudraniatricuspidatarootextract(CTE),thebloodalcoholmetabolismandbloodgasimbalanceofCTEinratstreatedwith10ml/kgalcoholwereexamined.CTE(500mg/kgand750mg/kg)wasadministratedafter30minutesofalcoholconsumption(10ml/kg).Bloodcollectionwasimplementedfromthetailsoftheanimalsafter1,3,and5hourspostalcoholconsumption.TheConditiondrink(acommercialanti-hangoverbeverage)wasusedasapositivecontrol.Singleadministrationbytheoralroutewasperformed.TheconsumptionofCTE(500mg/kgand750mg/kg)decreasedtheserumalcoholconcentrationbyincreasingandmaintainingboththealcoholdehydrogenase(ADH)andacetaldehydedehydrogenase(ALDH)enzymeactivitylevelsinthebloodandliver.Inaddition,CTEledtorecoveryfromtheimbalancesinthebloodgaslevels,includingcarbondioxide(CO2-)andchangesinpH,bicarbonate(HCO3-)andlacticacidlevelsduetoalcoholingestion.Inconclusion,CTEexertedamorepronouncedanti-hangovereffectthanacommercialanti-hangoverdrink.Therefore,CTEcanbeanovelandsafeanti-hangovernaturalproductagentforthepreventionortreatmentofsymptomscausedbyexcessivealcoholconsumption.
ProductionofBioethanolfromAgriculturalWastesUsingResidualThermalEnergyofaCogenerationPlantintheDistillationPhase.
Cutzu,R.&Bardi,L.(2017),3(2),24.
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Alcoholicfermentationswereperformedadaptingthetechnologytoexploittheresidualthermalenergy(hotwaterat83-85°C)ofacogenerationplantandtovalorizeagriculturalwastes.Substrateswereapple,kiwifruitandpeacheswastesandCornThreshingResidue(CTR).Saccharomycesbayanuswaschosenasbiocatalyst.Thefruits,freshorblanched,weremashed;CTRwasgelatinizedandliquefiedbyaddingLiquozyme®SCDS(Novozyme);saccharificationsimultaneoustofermentationwascarriedoutusingtheenzymeSpirizyme®Ultra(Novozyme).Lab-scalestaticfermentationswerecarriedoutat28°Cand35°C,usingrawfruits,blanchedfruitsandCTR,monitoringtheethanolproduction.ThehighestethanolproductionwasreachedwithCTR(10,22%9andamongfruitswithapple(8,71%).Distillationsatlowtemperaturesandundervacuum,toexploitwarmwaterfromcogenerationplant,weretested;distillationat80°Cand200mbaror400mbarallowedtorecover93,35and89,59%ofethanolrespectively.Theseresultssupportafermentationprocesscoupledtoacogenerationplant,fedwithapplewastesandwithCTRwhenapplewastesarenotavailable,wherehotwaterfromcogenerationplantisusedinblanchinganddistillationphases.Thescaleupinapilotplantwasalsocarriedout.
Duringyeastchronologicalagingresveratrolsupplementationresultsinashort-livedphenotypeSir2-dependent.
Orlandi,I.,Stamerra,G.,Strippoli,M.&Vai,M.(2017).RedoxBiology,12,745-754.
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Resveratrol(RSV)isanaturallyoccurringpolyphenoliccompoundendowedwithinterestingbiologicalproperties/functionsamongstwhichareitsactivityasanantioxidantandasSirtuinactivatingcompoundtowardsSIRT1inmammals.SirtuinscompriseafamilyofNAD+-dependentproteindeacetylasesthatareinvolvedinmanyphysiologicalandpathologicalprocessesincludingagingandage-relateddiseases.TheseenzymesareconservedacrossspeciesandSIRT1istheclosestmammalianorthologueofSir2ofSaccharomycescerevisiae.Inthefieldofagingresearches,itiswellknownthatSir2isapositiveregulatorofreplicativeLifespanand,inthiscontext,theRSVeffectshavebeenalreadyexamined.Here,weanalyzedRSVeffectsduringchronologicalaging,inwhichSir2actsasanegativeregulatorofchronologicallifespan(CLS).Chronologicalagingreferstoquiescentcellsinstationaryphase;thesecellsdisplayasurvival-basedmetabolismcharacterizedbyanincreaseinoxidativestress.WefoundthatRSVsupplementationattheonsetofchronologicalaging,namelyatthediauxicshift,increasesoxidativestressandsignificantlyreducesCLS.CLSreductionisdependentonSir2presencebothinexpiredmediumandinextremeCalorieRestriction.Inaddition,alldatapointtoanenhancementofSir2activity,inparticularSir2-mediateddeacetylationofthekeygluconeogenicenzymephosphoenolpyruvatecarboxykinase(Pck1).ThisleadstoareductionintheamountoftheacetylatedactiveformofPck1,whoseenzymaticactivityisessentialforgluconeogenesisandCLSextension.
TheBiorefineryConceptAppliedtoBioethanolandBiomethaneProductionfromManure.
Bona,D.,Vecchiet,A.,Pin,M.,Fornasier,F.,Mondini,C.,Guzzon,R.&Silvestri,S.WasteandBiomassValorization,1-11.
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Productionofbiofuelsfromfarmanimalwasterepresentsapromisingapproachtodiversifyinggreenenergyproductionandreducingcompetitionforcultivablelandsbetweenfuelandfood-orientedcrops.Thisworkwasaimedtodefinethetechnicalfeasibilityandthespecificsuitabilityofcattle,swineandpoultrymanuretointegratingbioethanolandbiomethaneproduction,usingthebiorefineryconcept.Saccharificationobtainedbydiluteacidpretreatment(3.5%H2SO4,121°C,30min)followedbyenzymatichydrolysisresultedintotalsugarrecoveryof230.16,160.40,and98.40mgg-1(ofdrymatter)forcattle,pig,andpoultrymanurerespectively.Thesugarwasthenfermentedusingfreeyeastco-cultures.Theethanolobtainedwas56.32mgg-1ofdrymatterforcattle(about52.59%ofthetheoreticalethanolyield);27.98mgg-1forswine(about88.66%ofthetheoreticalethanolyield);12.69mgg-1forpoultry(about31.32%ofthetheoreticalethanolyield).Methaneproductionfromdistillationwastewas72.95mgg−1fromdryrawfaecesforcattle,126.48mgg-1forswineand119.03mgg-1forpoultry.Cattlemanureshowedthebestenergybalanceintermsofethanolproductionwithabout824.16kJkg-1ofdryfaeces,butthetwointegratedprocessesgeneratedanetenergybalanceof1.28MJkg-1forcattle,4.57MJkg-1forswineand4.79MJkg-1forpoultry.
UV-methodforthedeterminationofEthanolinfoodstuffs,beverages,andothermaterials
Principle:
(alcoholdehydrogenase)
(1)Ethanol+NAD+→acetaldehyde+NADH+H+
(aldehydedehydrogenase)
(2)Acetaldehyde+NAD++H2O→aceticacid+NADH+H+
Kitsize: *60assays(manual)/600(microplate)
/600(auto-analyser)* Thenumberofmanualtestsperkitcanbedoubledifallvolumesarehalved.
ThiscanbereadilyaccommodatedusingtheMegaQuantTM Wave
Spectrophotometer(D-MQWAVE).Method: Spectrophotometricat340nm
Reactiontime: ~5min
Detectionlimit: 0.093mg/L
Applicationexamples:
Wine,beer,cider,alcoholicfruitjuices,spirits,liqueurs,low-alcoholic
/non-alcoholicbeverages,pickles,fruitandfruitjuice,chocolate
products,vinegar,jam,breadandbakeryproducts,honey,soysauce,
dairyproducts,cosmetics,pharmaceuticalsandothermaterials
(e.g.biologicalcultures,samples,etc.)
Methodrecognition:
MethodsbasedonthisprinciplehavebeenacceptedbyIFU,EBC,
MEBAKandASBC
Advantages
- Simpleformat–aldehydedehydrogenasesuppliedasstablesuspension
- Verycompetitiveprice(costpertest)
- Allreagentsstablefor>2yearsafterpreparation
- Rapidreaction
- Mega-Calc™softwaretoolisavailablefromourwebsiteforhassle-freerawdataprocessing
- Standardincluded
- Extendedcofactorsstability
- Suitableformanual,microplateandauto-analyserformats
品牌介绍
Megazyme品牌产品简介
来源：作者：人气：2149发表时间：2016-05-19 10:59:00【大 中 小】
Megazyme是一家全球性公司，专注于开发和提供用于饮料、谷物、乳制品、食品、饲料、发酵、生物燃料和葡萄酒产业用的分析试剂、酶和检测试剂盒。Megazyme的许多检测试剂盒产品已经为众多官方科学协会（包括AOAC, AACC , RACI, EBC和ICC等），经过严格的审核，批准认证为官方标准方法，确保以准确、可靠、定量和易于使用的测试方法，满足客户的质量诉求。
Megazyme的主要产品线包括：
◆ 检测试剂盒
◆ 酶
◆ 酶底物
◆ 碳水化合物
◆ 化学品/仪器
官网地址：http://www.megazyme.com
检测试剂盒特色产品：
货号
中文品名
用途
K-ACETAF
乙酸[AF法]检测试剂盒
酶法定量分析乙酸最广泛使用的方法
K-ACHDF
可吸收糖/膳食纤维检测试剂盒
酒精沉淀法测定膳食纤维
K-AMIAR
氨快速检测试剂盒
用于包括葡萄汁、葡萄酒以及其它食品饮料样品中氨含量的快速检测分析。
K-AMYL
直链淀粉/支链淀粉检测试剂盒
谷物淀粉和而粉中直链淀粉/支链淀粉比例和含量检测
K-ARAB
阿拉伯聚糖检测试剂盒
果汁浓缩液中阿拉伯聚糖的检测
K-ASNAM
L-天冬酰胺/L-谷氨酰胺和氨快速检测试剂盒
用于食品工业中丙烯酰胺前体、细胞培养基、以及上清液组分中、L-天冬酰胺，谷氨酰胺和氨的检测分析
K-ASPTM
阿斯巴甜检测试剂盒
专业用于测定饮料和食品中阿斯巴甜含量，操作简单
K-BETA3
β-淀粉酶检测试剂盒
适用于麦芽粉中β-淀粉酶的测定
K-BGLU
混合键β-葡聚糖检测试剂盒
测定谷物、荞麦粉、麦汁、啤酒及其它食品中混合键β-葡聚糖(1,3:1,4-β-D-葡聚糖)的含量
K-CERA
α-淀粉酶检测试剂盒
谷物和发酵液（真菌和细菌）中α-淀粉酶的分析测定
K-CITR
柠檬酸检测试剂盒
快速、可靠地检测食品、饮料和其它物料中柠檬酸（柠檬酸盐）含量
K-DLATE
乳酸快速检测试剂盒
快速、特异性检测饮料、肉类、奶制品和其它食品中L-乳酸和D-乳酸(乳酸盐）含量
K-EBHLG
酵母β-葡聚糖酶检测试剂盒
用于测量和分析酵母中1,3:1,6?-β-葡聚糖，也可以检测1,3-葡聚糖
K-ETSULPH
总亚硫酸检测试剂盒
测定葡萄酒、饮料、食品和其他物料中总亚硫酸含量（按二氧化硫计）的一种简单，高效，可靠的酶法检测方法
K-FRGLMQ
D-果糖/D-葡萄糖[MegaQuant法]检测试剂盒
适用于使用megaquant?色度计（505nm下）测定葡萄、葡萄汁和葡萄酒中D-果糖和D-葡萄糖的含量。
K-FRUC
果聚糖检测试剂盒
含有淀粉、蔗糖和其他糖类的植物提取物和食品中果聚糖的含量测定。
K-FRUGL
D-果糖/D-葡萄糖检测试剂盒
对植物和食品中果糖或葡萄糖含量的酶法紫外分光测定。
K-GALM
半乳甘露聚糖检测试剂盒
食品和植物产品中半乳甘露聚糖的含量检测
K-GLUC
D-葡萄糖[GOPOD]检测试剂盒
谷物提取物中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUHK
D-葡萄糖[HK]检测试剂盒
植物和食品中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUM
葡甘聚糖检测试剂盒
植物和食品中葡甘聚糖的含量测定。
K-INTDF
总膳食纤维检测试剂盒
总膳食纤维特定检测和分析
K-LACGAR
乳糖/D-半乳糖快速检测试剂盒
用于快速检测食品和植物产品中乳糖、D-半乳糖和L-阿拉伯糖
K-LACSU
乳糖/蔗糖/D-葡萄糖检测试剂盒
混合面粉和其它物料中蔗糖、乳糖和D-葡萄糖的测定
K-LACTUL
乳果糖检测试剂盒
特异性、快速和灵敏测量奶基样品中乳果糖含量
K-MANGL
D-甘露糖/D-果糖/D-葡萄糖检测试剂盒
适合测定植物产品和多糖酸性水解产物中D-甘露糖含量
K-MASUG
麦芽糖/蔗糖/D-葡萄糖检测试剂盒
在植物和食品中麦芽糖，蔗糖和葡萄糖的含量检测
K-PECID
胶质识别检测试剂盒
食品配料中果胶的鉴别
K-PHYT
植酸(总磷)检测试剂盒
食品和饲料样品植酸/总磷含量测量的简便方法。不需要通过阴离子交换色谱对植酸纯化，适合于大量样本分析
K-PYRUV
丙酮酸检测试剂盒
在啤酒、葡萄酒、果汁、食品和体液中丙酮酸分析
K-RAFGA
棉子糖/D-半乳糖检测试剂盒
快速测量植物材料和食品中棉子糖和半乳糖含量
K-RAFGL
棉子糖/蔗糖/D-半乳糖检测试剂盒
分析种子和种子粉中D-葡萄糖、蔗糖、棉子糖、水苏糖和毛蕊花糖含量。通过将棉子糖、水苏糖和毛蕊花糖酶解D-葡萄糖、D-果糖和半乳糖，从而测定葡萄糖含量来确定
K-SDAM
淀粉损伤检测试剂盒
谷物面粉中淀粉损伤的检测和分析
K-SUCGL
蔗糖/D-葡萄糖检测试剂盒
饮料、果汁、蜂蜜和食品中蔗糖和葡萄糖的分析
K-SUFRG
蔗糖/D-果糖/D-葡萄糖检测试剂盒
适用于植物和食品中蔗糖、D-葡萄糖和D-果糖的测定
K-TDFR
总膳食纤维检测试剂盒
总膳食纤维检测
K-TREH
海藻糖检测试剂盒
快速、可靠地检测食品、饮料和其它物料中海藻糖含量
K-URAMR
尿素/氨快速检测试剂盒
适用于水、饮料、乳制品和食品中尿素和氨的快速测定
K-URONIC
D-葡萄糖醛酸/D-半乳糖醛酸检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中六元糖醛酸含量（D-葡萄糖醛酸和D-半乳糖醛酸）
K-XYLOSE
D-木糖检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中D-木糖含量
K-YBGL
Beta葡聚糖[酵母和蘑菇]检测试剂盒
检测酵母和蘑菇制品中1,3:1,6-beta-葡聚糖和α-葡聚糖含量
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