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Megazyme/Acetic Acid Assay Kit (ACS Analyser Format)/K-ACETAF/141.6 mL of prepared reagent (e.g. 456 assays of 0.31 mL)
Megazyme/Acetic Acid Assay Kit (ACS Analyser Format)/K-ACETAF/141.6 mL of prepared reagent (e.g. 456 assays of 0.31 mL)
商品编号：
K-ACETAF
品牌：
Megazyme INC
市场价：
¥2980.00
美元价：
1788.00
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
TheAceticAcidanalyserformattestkitissuitableforthespecificmeasurementandanalysisofaceticacid(acetate)inbeveragesandfoodproducts.
EnhancedactivityofADPglucosepyrophosphorylaseandformationofstarchinducedbyAzospirillumbrasilenseinChlorellavulgaris.
Choix,F.J.,Bashan,Y.,Mendoza,A.&de-Bashan,L.E.(2014).JournalofBiotechnology,177,22-34.
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ADP-glucosepyrophosphorylase(AGPase)regulatesstarchbiosynthesisinhigherplantsandmicroalgae.ThisstudymeasuredtheeffectofthebacteriumAzospirillumbrasilenseonAGPaseactivityinthefreshwatermicroalgaChlorellavulgarisandformationofstarch.Thiswasdonebyimmobilizingbothmicroorganismsinalginatebeads,eitherrepletewithordeprivedofnitrogenorphosphorusandallunderheterotrophicconditions,usingD-glucoseorNa-acetateasthecarbonsource.AGPaseactivityduringthefirst72hofincubationwashigherinC.vulgariswhenimmobilizedwithA.brasilense.Thishappenedsimultaneouslywithhigherstarchaccumulationandhighercarbonuptakebythemicroalgae.Eithercarbonsourcehadsimilareffectsonenzymeactivityandstarchaccumulation.StarvationeitherbyNorPhadthesamepatternonAGPaseactivityandstarchaccumulation.Underrepleteconditions,thepopulationofC.vulgarisimmobilizedalonewashigherthanwhenimmobilizedtogether,butunderstarvationconditionsA.brasilenseinducedalargerpopulationofC.vulgaris.Insummary,addingA.brasilenseenhancedAGPaseactivity,starchformation,andmitigationofstressinC.vulgaris.
Enhancedaccumulationofstarchandtotalcarbohydratesinalginate-immobilizedChlorellaspp.inducedbyAzospirillumbrasilense:II.Heterotrophicconditions.
Choix,F.J.,de-Bashan,L.E.&Bashan,Y.(2012).EnzymeandMicrobialTechnology,51(5),300-309.
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TheeffectofthebacteriumAzospirillumbrasilensejointlyimmobilizedwithChlorellavulgarisorC.sorokinianainalginatebeadsontotalcarbohydratesandstarchwasstudiedunderdarkandheterotrophicconditionsfor144hinsyntheticgrowthmediumsupplementedwitheitherD-glucoseorNa-acetateascarbonsources.Inalltreatments,enhancedtotalcarbohydratesandstarchcontentpercultureandpercellwasobtainedafter24h;onlyjointlyimmobilizedC.vulgarisgrowingonD-glucosesignificantlyincreasedtotalcarbohydratesandstarchcontentafter96h.Enhancedaccumulationofcarbohydrateandstarchunderjointlyimmobilizedconditionswasvariablewithtimeofsamplingandsubstrateused.Similarresultsoccurredwhenthemicroalgaewasimmobilizedalone.Inbothmicroalgaegrowingoneithercarbonsources,thebacteriumpromotedaccumulationofcarbohydratesandstarch;whenthemicroalgaewereimmobilizedalone,theyusedthecarbonsourcesforcellmultiplication.InjointlyimmobilizedconditionswithChlorellaspp.,affinitytocarbonsourceandvolumetricproductivityandyieldwerehigherthanwhenChlorellaspp.wereimmobilizedalone;however,thegrowthratewashigherinmicroalgaeimmobilizedalone.Thisstudydemonstratesthatunderheterotrophicconditions,A.brasilensepromotestheaccumulationofcarbohydratesintwostrainsChlorellaspp.undercertaintime–substratecombinations,producingmainlystarch.Assuch,thisbacteriumisaBIOLOGicalfactorthatcanchangethecompositionofcompoundsinmicroalgaeindark,heterotrophicconditions.
DeletionofpyruvatedecarboxylasebyanewmethodforefficientMarkerlessgenedeletionsinGluconobacteroxydans.
Peters,B.,Junker,A.,Brauer,K.,Mühlthaler,B.,Kostner,D.,Mientus,M.,Liebl,W.&Ehrenreich,A.(2013).AppliedMicrobiologyandBiotechnology,97(6),2521-2530.
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Gluconobacteroxydans,abiotechnologicallyrelevantspecieswhichincompletelyoxidizesalargevarietyofcarbohydrates,alcohols,andrelatedcompounds,containsageneforpyruvatedecarboxylase(PDC).Thisenzymeisfoundonlyinveryfewspeciesofbacteriawhereitisnormallyinvolvedinanaerobicethanolformationviaacetaldehyde.InordertoclarifytheroleofPDCinthestrictlyoxidativemetabolismofaceticacidbacteria,wedevelopedamarkerlessin-framedeletionsystemforstrainG.oxydans621Hwhichuses5-fluorouraciltogetherwithaplasmid-encodeduracilphosphoribosyltransferaseascounterselectionmethodandusedthistechniquetodeletethePDCgene(GOX1081)ofG.oxydans621H.ThePDCdeletionmutantaccumulatedlargeamountsofpyruvatebutalmostnoacetateduringgrowthonD-mannitol,D-fructoseorinthepresenceofL-lactate.ThissuggestedthatinG.oxydansacetateformationoccursbydecarboxylationofpyruvateandsubsequentoxidationofacetaldehydetoacetate.Thisobservationandtheefficiencyofthemarkerlessdeletionsystemwereconfirmedbyconstructingdeletionmutantsoftwoacetaldehydedehydrogenases(GOX1122andGOX2018)andoftheacetyl-CoA-synthetase(GOX0412).Acetateformationduringgrowthofthesemutantsonmannitoldidnotdiffersignificantlyfromthewild-typestrain.
BiochemicalcharacterizationandrelativeexpressionlevelsofmultiplecarbohydrateesterasesofthexylanolyticrumenbacteriumPrevotellaruminicola23grownonanester-enrichedsubstrate.
Kabel,M.A.,Yeoman,C.J.,Han,Y.,Dodd,D.,Abbas,C.A.,deBont,J.A.M.,Morrison,M.,CannI.K.O.&Mackie,R.I.(2011).AppliedandEnvironmentalMicrobiology,77(16),5671-5681.
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WemeasuredexpressionandusedbiochemicalcharacterizationofmultiplecarbohydrateesterasesbythexylanolyticrumenbacteriumPrevotellaruminicola23grownonanester-enrichedsubstratetogaininsightintothecarbohydrateesteraseactivitiesofthishemicellulolyticrumenbacterium.TheP.ruminicola23genomecontains16genespredictedtoencodecarbohydrateesteraseactivity,andbasedonmicroarraydata,fourofthesewereupregulated>2-foldatthetranscriptionallevelduringgrowthonanester-enrichedoligosaccharide(XOSFA,AC)fromcornrelativetoanonesterifiedfractionofcornoligosaccharides(AXOS).Fourofthe16esterases(Xyn10D-Fae1A,Axe1-6A,AxeA1,andAxe7A),includingthetwomosthighlyinducedesterases(Xyn10D-Fae1AandAxe1-6A),wereheterologouslyexpressedinEscherichiacoli,purified,andbiochemicallycharacterized.AllfourenzymesshowedthehighestactivityatphysiologicallyrelevantpH(6to7)andtemperature(30to40°C)ranges.TheP.ruminicola23Xyn10D-Fae1A(acarbohydrateesterase[CE]family1enzyme)releasedferulicacidfrommethylferulate,wheatbran,cornfiber,andXOSFA,AC,acornfiber-derivedsubstrateenrichedinO-acetylandferulicacidesters,butexhibitednegligIBLeactivityonsugaracetates.Asexpected,theP.ruminicolaAxe1-6Aenzyme,whichwaspredictedtopossesstwodistinctesterasefamilydomains(CE1andCE6),releasedferulicacidfromthesamesubstratesasXyn10D-Fae1andwasalsoabletocleaveO-acetylesterbondsfromvariousacetylatedoligosaccharides(AcXOS).TheP.ruminicola23AxeA1,whichisnotassignedtoaCEfamily,andAxe7A(CE7)werefoundtobeacetylesterasesthathadactivitytowardabroadrangeofmostlynonpolymericacetylatedsubstratesalongwithAcXOS.Allenzymeswereinhibitedbytheproximallocationofothersidegroupslike4-O-methylglucuronicacid,ferulicacid,oracetylgroups.TheuniquediversityofcarbohydrateesterasesinP.ruminicola23likelygivesittheABIlitytohydrolyzesubstituentsonthexylanbackboneandenhancesitscapacitytoefficientlydegradehemicellulose.
Co-fermentationofacetateandsugarsfacilitatingmicrobiallipidproductiononacetate-richbiomasshydrolysates.
Gong,Z.,Zhou,W.,Shen,H.,Yang,Z.,Wang,G.,Zuo,Z.,Hou.Y.&Zhao,Z.K.(2016).Bioresourcetechnology,207,102-108.
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Theprocessoflignocellulosicbiomassroutinelyproducesastreamthatcontainssugarsplusvariousamountsofaceticacid.Asacetateisknowntoinhibitthecultureofmicroorganismsincludingoleaginousyeasts,littleattentionhasbeenpaidtoexplorelipidproductiononmixturesofacetateandsugars.HerewedemonstratedthattheyeastCryptococcuscurvatuscaneffectivelyco-fermentacetateandsugarsforlipidproduction.Whenmixturesofacetateandglucosewereapplied,C.curvatusconsumedbothsubstratessimultaneously.Similarphenomenawerealsoobservedforacetateandxylosemixtures,aswellasacetate-richcornstoverhydrolysates.Moreinterestingly,thereplacementofsugarwithequalamountofacetateascarbonsourceaffordedhigherlipidtitreandlipidcontent.Thelipidproductshadfattyacidcompositionalprofilessimilartothoseofcocoabutter,suggestingtheirpotentialforhighvalue-addedfatsandbiodieselproduction.Thisco-fermentationstrategyshouldfacilitatelipidproductiontechnologyfromlignocelluloses.
InteractionofAzospirillumspp.withmicroalgae:abasiceukaryotic-prokaryoticmodelanditsbiotechnologicalapplications.
de-Bashan,L.E.,Hernandez,J.P.&Bashan,Y.(2015).“HandbookforAzospirillum”,SpringerInternationalPublishing,367-388.
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TheinteractionofthebacteriaAzospirillumspp.withphotosynthetic,singlecellmicroalgaethatareco-immobilizedinalginatebeadsprovidesasignificantshortcutforunderstandingtheinteractionofthisplantgrowth-promotingbacteria(PGPB)withplantsingeneral.Thisinteractioniscurrentlyrelevantforstudyingphysiological,physical,biochemical,andmolecularaspects.AsanindependentsubfieldofAzospirillumresearch,thisinteractionhassomesignificantpotentialbiotechnologicalapplications,suchaswastewatertreatment,productionofbiofuel(ethanolandbiodiesel),increasedfertilityoferodedsoilscombinedwithpromotinggrowthofhigherplants,productionofpigments,andproductionofbiomass.Alloftheseapplicationshaveyettobescaledupandevaluatedfortheirtruepracticalpotential.
AnalyserformatUV-methodforthedeterminationofAceticAcid
infoodstuffs,beveragesandothermaterials
Principle:
(acetyl-CoAsynthetase)
(1)Aceticacid+ATP+CoA→acetyl-CoA+AMP+ pyrophosphate
(citratesynthase)
(2)Acetyl-CoA+oxaloacetate+H2O→citrate+CoA
(L-malatedehydrogenase)
(3)L-Malate+NAD+↔oxaloacetate+NADH+H+
Kitsize: 141.6mLofpreparedreagent(R1+R2)
Method: Spectrophotometricat340nm
Reactiontime: ~15min
Detectionlimit: 10mg/L(recommendedassayformat)
Applicationexamples:
Wine,beer,fruitandfruitjuices,softdrinks,vinegar,vegetables,
pickles,dairyproducts(e.g.cheese),meat,fish,bread,bakeryproducts
(andbakingagents),ketchup,soysauce,mayonnaise,dressings,
paper(andcardboard),tea,pharmaceuticals(e.g.infusionsolutions),
feedandothermaterials(e.g.biologicalcultures,samples,etc.)
Methodrecognition:
MethodsbasedonthisprinciplehavebeenacceptedbyEN,ISO,
ICUMSA,IFUandMEBAK
Advantages
- NowastedACSsolution(stablesUSPensionsupplied)
- PVPincorporatedtopreventtannininhibition
- Verystablereagentwhenpreparedforauto-analyserapplications(>3daysat4°C)
- Linearcalibrationupto30μg/mLofaceticacidinfinalreactionsolution
- ValidatedbytheUniversityofWine,SuzelaRousse,France
- Verycompetitiveprice(costpermLofreagent)
- Allreagentsstablefor>2yearsafterpreparation
品牌介绍
Megazyme品牌产品简介
来源：作者：人气：2149发表时间：2016-05-19 10:59:00【大 中 小】
Megazyme是一家全球性公司，专注于开发和提供用于饮料、谷物、乳制品、食品、饲料、发酵、生物燃料和葡萄酒产业用的分析试剂、酶和检测试剂盒。Megazyme的许多检测试剂盒产品已经为众多官方科学协会（包括AOAC, AACC , RACI, EBC和ICC等），经过严格的审核，批准认证为官方标准方法，确保以准确、可靠、定量和易于使用的测试方法，满足客户的质量诉求。
Megazyme的主要产品线包括：
◆ 检测试剂盒
◆ 酶
◆ 酶底物
◆ 碳水化合物
◆ 化学品/仪器
官网地址：http://www.megazyme.com
检测试剂盒特色产品：
货号
中文品名
用途
K-ACETAF
乙酸[AF法]检测试剂盒
酶法定量分析乙酸最广泛使用的方法
K-ACHDF
可吸收糖/膳食纤维检测试剂盒
酒精沉淀法测定膳食纤维
K-AMIAR
氨快速检测试剂盒
用于包括葡萄汁、葡萄酒以及其它食品饮料样品中氨含量的快速检测分析。
K-AMYL
直链淀粉/支链淀粉检测试剂盒
谷物淀粉和而粉中直链淀粉/支链淀粉比例和含量检测
K-ARAB
阿拉伯聚糖检测试剂盒
果汁浓缩液中阿拉伯聚糖的检测
K-ASNAM
L-天冬酰胺/L-谷氨酰胺和氨快速检测试剂盒
用于食品工业中丙烯酰胺前体、细胞培养基、以及上清液组分中、L-天冬酰胺，谷氨酰胺和氨的检测分析
K-ASPTM
阿斯巴甜检测试剂盒
专业用于测定饮料和食品中阿斯巴甜含量，操作简单
K-BETA3
β-淀粉酶检测试剂盒
适用于麦芽粉中β-淀粉酶的测定
K-BGLU
混合键β-葡聚糖检测试剂盒
测定谷物、荞麦粉、麦汁、啤酒及其它食品中混合键β-葡聚糖(1,3:1,4-β-D-葡聚糖)的含量
K-CERA
α-淀粉酶检测试剂盒
谷物和发酵液（真菌和细菌）中α-淀粉酶的分析测定
K-CITR
柠檬酸检测试剂盒
快速、可靠地检测食品、饮料和其它物料中柠檬酸（柠檬酸盐）含量
K-DLATE
乳酸快速检测试剂盒
快速、特异性检测饮料、肉类、奶制品和其它食品中L-乳酸和D-乳酸(乳酸盐）含量
K-EBHLG
酵母β-葡聚糖酶检测试剂盒
用于测量和分析酵母中1,3:1,6?-β-葡聚糖，也可以检测1,3-葡聚糖
K-ETSULPH
总亚硫酸检测试剂盒
测定葡萄酒、饮料、食品和其他物料中总亚硫酸含量（按二氧化硫计）的一种简单，高效，可靠的酶法检测方法
K-FRGLMQ
D-果糖/D-葡萄糖[MegaQuant法]检测试剂盒
适用于使用megaquant?色度计（505nm下）测定葡萄、葡萄汁和葡萄酒中D-果糖和D-葡萄糖的含量。
K-FRUC
果聚糖检测试剂盒
含有淀粉、蔗糖和其他糖类的植物提取物和食品中果聚糖的含量测定。
K-FRUGL
D-果糖/D-葡萄糖检测试剂盒
对植物和食品中果糖或葡萄糖含量的酶法紫外分光测定。
K-GALM
半乳甘露聚糖检测试剂盒
食品和植物产品中半乳甘露聚糖的含量检测
K-GLUC
D-葡萄糖[GOPOD]检测试剂盒
谷物提取物中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUHK
D-葡萄糖[HK]检测试剂盒
植物和食品中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUM
葡甘聚糖检测试剂盒
植物和食品中葡甘聚糖的含量测定。
K-INTDF
总膳食纤维检测试剂盒
总膳食纤维特定检测和分析
K-LACGAR
乳糖/D-半乳糖快速检测试剂盒
用于快速检测食品和植物产品中乳糖、D-半乳糖和L-阿拉伯糖
K-LACSU
乳糖/蔗糖/D-葡萄糖检测试剂盒
混合面粉和其它物料中蔗糖、乳糖和D-葡萄糖的测定
K-LACTUL
乳果糖检测试剂盒
特异性、快速和灵敏测量奶基样品中乳果糖含量
K-MANGL
D-甘露糖/D-果糖/D-葡萄糖检测试剂盒
适合测定植物产品和多糖酸性水解产物中D-甘露糖含量
K-MASUG
麦芽糖/蔗糖/D-葡萄糖检测试剂盒
在植物和食品中麦芽糖，蔗糖和葡萄糖的含量检测
K-PECID
胶质识别检测试剂盒
食品配料中果胶的鉴别
K-PHYT
植酸(总磷)检测试剂盒
食品和饲料样品植酸/总磷含量测量的简便方法。不需要通过阴离子交换色谱对植酸纯化，适合于大量样本分析
K-PYRUV
丙酮酸检测试剂盒
在啤酒、葡萄酒、果汁、食品和体液中丙酮酸分析
K-RAFGA
棉子糖/D-半乳糖检测试剂盒
快速测量植物材料和食品中棉子糖和半乳糖含量
K-RAFGL
棉子糖/蔗糖/D-半乳糖检测试剂盒
分析种子和种子粉中D-葡萄糖、蔗糖、棉子糖、水苏糖和毛蕊花糖含量。通过将棉子糖、水苏糖和毛蕊花糖酶解D-葡萄糖、D-果糖和半乳糖，从而测定葡萄糖含量来确定
K-SDAM
淀粉损伤检测试剂盒
谷物面粉中淀粉损伤的检测和分析
K-SUCGL
蔗糖/D-葡萄糖检测试剂盒
饮料、果汁、蜂蜜和食品中蔗糖和葡萄糖的分析
K-SUFRG
蔗糖/D-果糖/D-葡萄糖检测试剂盒
适用于植物和食品中蔗糖、D-葡萄糖和D-果糖的测定
K-TDFR
总膳食纤维检测试剂盒
总膳食纤维检测
K-TREH
海藻糖检测试剂盒
快速、可靠地检测食品、饮料和其它物料中海藻糖含量
K-URAMR
尿素/氨快速检测试剂盒
适用于水、饮料、乳制品和食品中尿素和氨的快速测定
K-URONIC
D-葡萄糖醛酸/D-半乳糖醛酸检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中六元糖醛酸含量（D-葡萄糖醛酸和D-半乳糖醛酸）
K-XYLOSE
D-木糖检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中D-木糖含量
K-YBGL
Beta葡聚糖[酵母和蘑菇]检测试剂盒
检测酵母和蘑菇制品中1,3:1,6-beta-葡聚糖和α-葡聚糖含量
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