Megazyme/醋酸分析试剂盒（醋酸激酶分析仪格式）/K-乙缩醛K/170.5 mL制备的试剂（例如550次分析
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K-ACETAK
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Megazyme INC
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¥3320.00
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1992.00
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其它检测试剂盒
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Other_kits
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3392242852
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4000-520-616
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info@ebiomall.com
商品介绍
TheAceticAcidanalyserformattestkitissuitableforthespecificmeasurementandanalysisofaceticacid(acetate)inbeveragesandfoodproducts.Oncalibration,thepreparedreagentislinearto>28microgramsofaceticacidpermLofassaysolution.
Grapeandwineanalysis:Oenologiststoexploitadvancedtestkits.
Charnock,S.C.&McCleary,B.V.(2005).RevuedesEnology,117,1-5.
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Itiswithoutdoubtthattestingplaysapivotalrolethroughoutthewholeofthevinificationprocess.ToproducethebestpossIBLequalitywineandtominimiseprocessproblemssuchas“stuck”fermentationortroublesomeinfections,itisnowrecognisedthatifpossibletestingshouldbeginpriortoharvestingofthegrapesandcontinuethroughtobottling.TrADItionalmethodsofwineanalysisareoftenexpensive,timeconsuming,requireeitherelaborateequipmentorspecialistexpertiseandfrequentlylackaccuracy.However,enzymaticbio-analysisenablestheaccuratemeasurementofthevastmajorityofanalytesofinteresttothewinemaker,usingjustonepieceofapparatus,thespectrophotometer(seepreviousissueNo.116foradetailedtechnicalreview).Grapejuiceandwineareamenabletoenzymatictestingasbeingliquidstheyarehomogenous,easytomanipulate,andcangenerallybeanalysedwithoutanysamplepreparation.
Megazyme“advanced”winetestkitsgeneralcharacteristicsandvalidation.
Charnock,S.J.,McCleary,B.V.,Daverede,C.&Gallant,P.(2006).ReveuedesOenologues,120,1-5.
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ManyoftheenzymatictestkitsareofficialmethodsofprestigiousorganisationssuchastheAssociationofOfficialAnalyticalChemicals(AOAC)andtheAmericanAssociationofCerealChemists(AACC)inresponsetotheinterestfromoenologists.Megazymedecidedtouseitslonghistoryofenzymaticbio-analysistomakeasignificantcontributiontothewineindustry,bythedevelopmentofarangeofadvancedenzymatictestkits.Thistaskhasnowbeensuccessfullycompletedthroughthestrategicandcomprehensiveprocessofidentifyinglimitationsofexistingenzymaticbio-analysistestkitswheretheyoccurred,andthenusingadvancedtechniques,suchasmolecularBIOLOGy(photo1),torapidlyovercomethem.Noveltestkitshavealsobeendevelopedforanalytesofemerginginteresttotheoenologist,suchasyeastavailablenitrogen(YAN;seepages2-3ofissue117article),orwherepreviouslyenzymesweresimplyeithernotavailable,orweretooexpensivetoemploy,suchasforD-mannitolanalysis.
Microbialbioanodeswithhighsalinitytoleranceformicrobialfuelcellsandmicrobialelectrolysiscells.
Rousseau,R.,Dominguez-Benetton,X.,Délia,M.L.&Bergel,A.(2013).ElectRochemistryCommunications,33,1-4.
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Increasingtheconductivityoftheelectrolytesusedinmicrobialelectrochemicalsystemsisanessentialprerequisitetothelarge-scalesuccessofthesetechnologies.Microbialbioanodesformedfromasaltmarshinoculumunderconstantacetatefeedinggeneratedupto85A•m-2inmediacontaining776mMNaCl(45g•L-1,1.5timesthesalinityofseawater).Thesevalueswerethehighestsalinitiesacceptedbyamicrobialanodesofarandthehighestcurrentdensitiesreportedwithfeltgraphiteelectrodes.
Theeffectofpathogenreductiontechnology(Mirasol)onplateletqualitywhentreatedinadditivesolutionwithlowplasmacarryover.
Johnson,L.,Winter,K.M.,Reid,S.,Hartkopf‐Theis,T.,Marschner,S.,Goodrich,R.P.&Marks,D.C.(2011).Voxsanguinis,101(3),208-214.
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BackgroundandObjectives:Pathogenreductiontechnologies(PRT)forplateletsarenowcompatiblewithbothplasmaandplateletadditivesolutions(PAS).TheaimofthisstudywastoexaminetheeffectofPRTontheplateletstoragelesion,inthepresenceofPASwithlowplasmacarryover.MaterialsandMethods:PRT-treated(Mirasol)anduntreatedbuffycoat-derivedplateletconcentratespreparedin28%plasma/PAS-IIIMwereevaluatedusinginvitrocellqualityparametersondays1,2,5,and7post-collection.Results:Atday5,therewerenosignificantdifferencesbetweencontrolandPRTtreatedplateletsforswirl,viABIlity,pO2,pCO2,meanplateletvolumeandadenosinediphosphate-inducedaggregation.PRTtreatmentdidnotaffectthefunctionalintegrityofthemitochondria.However,PRTresultedinadecreaseinpHandenhancementofplateletglycolysisandactivation,evidencedbyincreasedglucoseconsumptionandlactateproductionrates,increasedexpressionofCD62P,CD63,annexinVstainingandincreasedsecretionofcytokines(P<0•05).=""hypotonic=""shock=""response=""and=""aggregation=""in=""response=""to=""collagen=""were=""also=""significantly=""reduced=""in=""prt=""treated=""platelets="">P<0•05).="">Conclusion:DespitetheobserveddifferencesinplateletmetabolismandactivationobservedfollowingPRTtreatmentinPASandlowplasmacarryover,theresultssuggestthattreatmentandstorageofplateletsinPASisnomoredetrimentaltoplateletsthantreatmentandstorageinplasma.
Invitroassessmentofbuffy‐coatderivedplateletcomponentssUSPendedinSSP+treatedwiththeINTERCEPTBloodsystem.
Johnson,L.,Loh,Y.S.,Kwok,M.&Marks,D.C.(2013).TransfusionMedicine,23(2),121-129.
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Background:TheINTERCEPTBloodSystemusesamotosalen-HClandUVAlighttocross-linkDNAandRNA,therebyinhibitingpathogenreplication.Althoughpreviousstudieshaveshownthatthistreatmentaltersinvitroplateletquality,moststudieshaveassessedapheresisplateletsorplateletspooledfrom5or6donors.InAustralia,plateletsarepreparedusingbuffy-coatsfrom4donors,withSSP+andhavelowerplasmacarryoverthanrecommendedbythemanufacturer(32–47%).Assuch,itiscurrentlyunknownwhethertheseplateletconcentratesaresuitableforINTERCEPTtreatment.Materialsandmethods:Plateletconcentrateswerepreparedbypoolingfourbuffy-coatswithSSP+,resultingin30%plasmacarryover.Twoplateletunitswerepooledandsplittogeneratepairedunits,withoneunittreatedwiththeINTERCEPTSystem(n = 6),whilsttheotherremaineduntreated(n = 6).Allunitswerestoredforsevendaysat22°Cwithagitation.Results:INTERCEPTtreatmentresultedin10•4 ± 4•3%lossofplatelets,butdidnotsignificantlyaffectthefunctionalintegrityofmitochondria.INTERCEPT-treatedplateletsdemonstratedadecreasedpH,acceleratedlactateproductionandglucoseconsumption,aswellashighersurfaceexpressionandincreasedsecretionofP-selectinandreducedcollagen-inducedaggregation.Thesechangeswereparticularlyevidentfromday5ofstorage.Conclusion:TheobservedincreaseinplateletglycolysisfollowingINTERCEPTtreatmentisconsistentwithpreviousliteraturereports.Importantly,theinvitrochangeswerelessmarkedthanpreviouslyreportedindicatingthattheplateletssuspendedinSSP+withreducedplasmacarryoverareofsuitableinvitroqualityfollowingINTERCEPTtreatmentandstorage.
Epistasisandfrequencydependenceinfluencethefitnessofanadaptivemutationinadiversifyinglineage.
LeGac,M.&Doebeli,M.(2010).MolecularEcology,19(12),2430-2438.
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Theopportunityforamutationtoinvadeapopulationcandramaticallyvarydependingonthecontextinwhichthismutationoccurs.Suchcontextdependenceisdifficulttodocumentasitrequirestheabilitytomeasurehowamutationaffectsphenotypesandfitnessandtomanipulatethecontextinwhichthemutationoccurs.Weidentifiedamutationinageneencodingaglobalregulatorinoneoftwoecotypesthatdivergedfromacommonancestorduring1200generationsofexperimentalevolution.Wereplacedtheancestralallelebythemutantallele,andviceversa,inseveralclonesisolatedduringthetimecourseoftheevolutionexperiment,andcomparedthephenotypeandfitnessofclonesisogenicexceptforthefocalmutation.Weshowthatthefitnessandphenotypeofthemutationarestronglyaffectedbyepistaticinteractionsbetweengenesinthesamegenome,aswellasbyfrequencydependentselectionresultingfrombioticinteractionsbetweenindividualsinthesamepopulation.Weconcludethatamongstthereplicatepopulationinwhichitspread,themutationweidentifiedisonlyadaptivewhenoccurringinspecificgenomesandcompetingwithspecificindividuals.Thisstudythusdemonstratesthattheopportunityforanadaptivemutationtospreadinanevolutionarylineagecanonlybeunderstoodinthelightofitsgenomicandcompetitiveenvironments.
ProductionoffunctionalizedbiopolyestergranulesbyrecombinantLactococcuslactis.
Mifune,J.,Grage,K.&Rehm,B.H.A.(2009).AppliedandEnvironmentalMicrobiology,75(14),4668-4675.
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Manybacteriaarenaturallycapableofaccumulatingbiopolyesterscomposedof3-hydroxyfattyacidsasintracellularinclusions,whichserveasstoragegranules.Recently,theseinclusionshavebeenconsideredasnano-/microbeadswithsurface-attachedproteins,whichcanbeengineeredtodisplayvariousprotein-basedfunctionsthataresuitableforbiotechnologicalandbiomedicalapplications.Inthisstudy,thefood-grade,generally-regarded-as-safegram-positiveorganismLactococcuslactiswasengineeredtorecombinantlyproducethebiopolyesterpoly(3-hydroxybutyrate)andtherespectiveintracellularinclusions.Thecodon-optimizedpolyhydroxybutyratebiosynthesisoperonphaCABfromCupriavidusnecatorwasexpressedusingthenisin-controlledgeneexpressionsystem.RecombinantL.lactisaccumulatedupto6%(wt/wt)poly(3-hydroxybutyrate)ofcellulardryweight.Poly(3-hydroxybutyrate)granuleswereisolatedandanalyzedwithrespecttoboundproteinsusingbiochemicalmethodsandwithrespecttoshape/sizeusingtransmissionelectronmicroscopy.TheimmunoglobulinG(IgG)bindingZZdomainofStaphylococcusaureusproteinAwaschosenasanexemplaryfunctionalitytobedisplayedatthegranulesurfacebyfusingittotheNterminusofthegranule-associatedpoly(3-hydroxybutyrate)synthase.Thepresenceofthefusionproteinatthesurfaceofisolatedgranuleswasconfirmedbypeptidefingerprintingusingmatrix-assistedlaserdesorptionionization-timeofflight(massspectrometry).ThefunctionalityoftheZZdomain-displayinggranuleswasdemonstratedbyenzyme-linkedimmunosorbentassayandIgGaffinitypurification.Inbothassays,theZZbeadsfromrecombinantL.lactisperformedatleastequallytoZZbeadsfromEscherichiacoli.Overall,inthisstudyitwasshownthatrecombinantL.lactiscanbeusedtomanufactureendotoxin-freepoly(3-hydroxybutyrate)beadswithsurfacefunctionalitiesthataresuitableforbiomedicalapplications.
Acetylesterase-mediateddeacetylationofpectinimpairscellelongation,pollengermination,andplantreproduction.
Gou,J.Y.,Miller,L.M.,Hou,G.,Yu,X.H.,Chen,X.Y.&Liu,C.J.(2012).ThePlantCell,24(1),50-65.
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Pectinisamajorcomponentoftheprimarycellwallofhigherplants.SomegalacturonylresiduesinthebackboneofpectinaceouspolysaccharidesareoftenO-acetylatedattheC-2orC-3position,andtheresultingacetylesterschangedynamicallyduringthegrowthanddevelopmentofplants.Theprocessesinvolvebothenzymaticacetylationanddeacetylation.Throughgenomicsequenceanalysis,weidentifiedapectinacetylesterase(PAE1)fromblackcottonwood(Populustrichocarpa).RecombinantPtPAE1exhibitedpreferentialactivityinreleasingtheacetatemoietyfromsugarbeet(Betavulgaris)andpotato(Solanumtuberosum)pectininvitro.OverexpressingPtPAE1intobacco(Nicotianatabacum)decreasedthelevelofacetylestersofpectinbutnotofxylan.Deacetylationengendereddifferentialchangesinthecompositionand/orstructureofcellwallpolysaccharidesthatsubsequentlyimpairedthecellularelongationoffloralstylesandfilaments,thegerminationofpollengrains,andthegrowthofpollentubes.Consequently,plantsoverexpressingPAE1exhibitedseveremalesterility.FurThermore,incontrasttotheconventionalview,PAE1-mediateddeacetylationsubstantiallyloweredthedigestibilityofpectin.Ourdatasuggeststhatpectinacetylesterasefunctionsasanimportantstructuralregulatorinplantsbymodulatingtheprecisestatusofpectinacetylationtoaffecttheremodelingandphysiochemicalpropertiesofthecellwall"spolysaccharides,therebyaffectingcellextensibility.
Gardencompostinoculumleadstomicrobialbioanodeswithpotential-independentcharacteristics.
Cercado,B.,Byrne,N.,Bertrand,M.,Pocaznoi,D.,Rimboud,M.,Achouak,W.&Bergel,A.(2013).BioresourceTechnology,134,276-284.
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Gardencompostleachatewasusedtoformmicrobialbioanodesunderpolarizationat−0.4,−0.2and+0.1V/SCE.Currentdensitieswere6.3and8.9Am-2onaverageat−0.4and+0.1V/SCErespectively,withacetate10mM.Thecatalyticcyclicvoltammetry(CV)showedsimilarelectrochemicalcharacteristicsforallbioanodesandindicatedthatthelowercurrentsrecordedat−0.4V/SCEwereduetotheslowerinterfacialelectrontransferrateatthispotential,consistentlywithconventionalelectrochemicalkinetics.RNA-andDNA-basedDGGEevidencedthatthethreedominantbacterialgroupsGeobacter,AnaerophagaandPelobacterwereidenticalforallbioanodesanddidnotdependonthepolarizationpotential.Onlynon-turnoverCVsshoweddifferencesintheredoxequipmentofthebiofilms,thehighestpotentialpromotingmultipleelectrontransferpathways.Thisfirstdescriptionofapotential-independentelectroactivemicrobialcommunityopensuppromisingProspectsforthedesignofstablebioanodesformicrobialfuelcells.
CombinedintracellularnitrateandNIT2effectsonstoragecarbohydratemetabolisminChlamydomonas.
Remacle,C.,Eppe,G.,Coosemans,N.,Fernandez,E.&Vigeolas,H.(2014).JournalofExperimentalBotany,65(1),23-33.
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Microalgaearereceivingincreasingattentionasalternativeproductionsystemsforrenewableenergysuchasbiofuel.ThephotosyntheticalgaChlamydomonasreinhardtiiiswidelyrecognizedasthemodelsystemtostudyallaspectsofalgalphysiology,includingthemolecularmechanismsunderlyingtheaccumulationofstarchandtriacylglycerol(TAG),whicharetheprecursorsofbiofuel.Allofthesepathwaysnotonlyrequireacarbon(C)supplybutalsoarestronglydependentonasourceofnitrogen(N)tosustainoptimalgrowthrateandbiomassproduction.InordertogainabetterunderstandingoftheregulationofCandNmetabolismsandtheaccumulationofstoragecarbohydrates,theeffectofdifferentNsources(NH4NO3andNH4+)onprimarymetabolismusingvariousmutantsimpairedineitherNIA1,NIT2orbothlociwasperformedbymetabolicanalyses.Thedatademonstratedthat,usingNH4NO3,nia1straindisplayedthemoststrikingphenotype,includinganinhibitionofgrowth,accumulationofintracellularnitrate,andstrongstarchandTAGaccumulation.ThemeasurementsofthedifferentCandNintermediatelevels(amino,organic,andfattyacids),togetherwiththedeterminationofacetateandNH4+remaininginthemedium,clearlyexcludedthehypothesisofaslowerNH4+andacetateassimilationinthismutantinthepresenceofNH4NO3.TheresultsprovideevidenceoftheimplicationofintracellularnitrateandNIT2inthecontrolofCpartitioningintodifferentstoragecarbohydratesundermixotrophicconditionsinChlamydomonas.Theunderlyingmechanismsandimplicationsforstrategiestoincreasebiomassyieldandstorageproductcompositioninoleaginousalgaearediscussed.
IndependentBenthicMicrobialFuelCellsPoweringSensorsandAcousticCommunicationswiththeMARSUnderwaterObservatory.
Schrader,P.S.,Reimers,C.E.,Girguis,P.,Delaney,J.,Doolan,C.,Wolf,M.&Green,D.(2016).JournalofAtmosphericandOceanicTechnology,33(3),607-617.
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Mostoceanographicinstrumentsontheseafloorhavenoconnectionswiththesurfaceandthereforehavetorunonbatteriesandstoredatauntilrecovery.Todemonstrateadevelopingtechnology,sensorsandacousticmodemswerepoweredwithenergyharvestedfromtheseafloor,anddatawererelayedacousticallyinnear–realtimetotheMontereyAcceleratedResearchSystem(MARS)observatoryinMontereyBay,California,andtosurfaceresearchvessels.MARSisacabledobservatoryindeepwater(~890m)attheedgeofMontereyCanyon.AnacousticmodemwasattachedtotheMARSnodeandconfiguredtosendoutcommandsto,andrelaydatareceivedfrom,remotemodems.Twobenthicmicrobialfuelcells(BMFCs)positionedapproximately0.5kmawayfromMARSsuppliedpowertotheremotemodemsandsensors.Attheirpeakperformance,theseBMFCsproducedcontinuouspowerdensitiesof~35mWm-2(footprintarea).Themodemsutilizedinthisstudycontainedanintegratedpowermanagementplatform(PMP)designedtomanageandstoretheelectricalenergygeneratedbyeachBMFCandtorecordBMFCperformanceparametersandsensordataonanhourlybasis.Temperatureandeitheroxygenorconductivitysensorswerechosenbecauseoftheircommonuseandenvironmentalrelevance.AcousticallytransmitteddatarecordsshowthattheBMFCsrenewedenergystoresandthattheoceanographicsensorsmeasureddissolvedoxygen,temperature,andconductivityreliablythroughouttheoperationallifeofeachBMFCsystem(~6months).Thesesystemsremainedinplaceformorethan12months.
Halotolerantbioanodes:Theappliedpotentialmodulatestheelectrochemicalcharacteristics,thebiofilmstructureandtheratioofthetwodominantgenera.
Rousseau,R.,Santaella,C.,Bonnafous,A.,Achouak,W.,Godon,J.J.,Delia,M.L.&Bergel,A.(2016).Bioelectrochemistry,112,24-32.
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Thedevelopmentofeconomically-efficientmicrobialelectrochemicaltechnologiesremainshinderedbythelowionicconductivityoftheculturemediausedastheelectrolyte.Toovercomethisdrawback,halotolerantbioanodesweredesignedwithsaltmarshsedimentusedastheinoculuminelectrolytescontainingNaClat30or45 g/L(ionicconductivity7.0or10.4 S·m-1).Thebioanodeswereformedatfourdifferentpotentials− 0.4,− 0.2,0.0and0.2 V/SCEtoidentifytheeffectontheelectrochemicalkineticparameters,thebiofilmstructuresandthecompositionofthemicrobialcommunities.Thebioanodesformedat− 0.4 V/SCEwerelargelydominatedbyMarinobacterspp.Voltammetryshowedthattheyprovidedhighercurrentsthantheotherbioanodesintherangeoflowpotentials,butthemaximumcurrentswerelimitedbythepoorsurfacecolonization.Thebioanodesformedat− 0.2,0.0and0.2 V/SCEshowedsimilarratiosofMarinobacterandDesulfuromonasspp.andhighervaluesofthemaximumcurrentdensity.Thecombinedanalysisofkineticparameters,biofilmstructureandbiofilmcompositionshowedthatMarinobacterspp.,whichensuredahigherelectrontransferrate,werepromisingspeciesforthedesignofhalotolerantbioanodes.ThechallengeisnowtoovercomeitslimitedsurfacecolonizationintheabsenceofDesulfuromonasspp.
Novelroleforcarbohydrateresponsiveelementbindingproteininthecontrolofethanolmetabolismandsusceptibilitytobingedrinking.
Marmier,S.,Dentin,R.,Daujat‐Chavanieu,M.,Guillou,H.,Bertrand‐Michel,J.,Gerbal‐Chaloin,S.,Girard,J.,Lotersztajn,S.&Postic,C.(2015).Hepatology,62(4),1086-1100.
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Carbohydrateresponsiveelementbindingprotein(ChREBP)iscentralfordenovofattyacidsynthesisunderphysiologicalconditionsandinthecontextofnonalcoholicfattyliverdisease.Weexploreditscontributiontoalcohol-inducedsteatosisinamousemodelofbingedrinkingasacuteethanol(EtOH)intoxicationhasbecomeanalarminghealthproblem.Within6hours,ChREBPacetylationanditsrecruitmentontotargetgenepromoterswereincreasedinliverofEtOH-fedmice.AcetylationofChREBPwasdependentonalcoholmetabolismbecauseinhibitionofalcoholdehydrogenase(ADH)activitybluntedChREBPEtOH-inducedacetylationinmousehepatocytes.Transfectionofanacetylation-defectivemutantofChREBP(ChREBPK672A)inHepG2cellsimpairedthestimulatoryeffectofEtOHonChREBPactivity.Importantly,ChREBPsilencingintheliverofEtOH-fedmicepreventedalcohol-inducedtriglycerideaccumulationthroughaninhibitionofthelipogenicpathwaybutalsoled,unexpectedly,tohypothermia,increasedbloodacetaldehydeconcentrations,andenhancedlethality.ThisphenotypewasassociatedwithimpairedhepaticEtOHmetabolismasaconsequenceofreducedADHactivity.WhiletheexpressionandactivityoftheNAD+dependentdeacetylasesirtuin1,aChREBP-negativetarget,weredown-regulatedintheliverofalcohol-fedmice,theywererestoredtocontrollevelsuponChREBPsilencing.Inturn,ADHacetylationwasreduced,suggestingthatChREBPregulatesEtOHmetabolismandADHactivitythroughitsdirectcontrolofsirtuin1expression.Indeed,whensirtuin1activitywasrescuedbyresveratrolpretreatmentinEtOH-treatedhepatocytes,asignificantdecreaseinADHproteincontentand/oracetylationwasobserved.Conclusion:ourstudydescribesanovelroleforChREBPinEtOHmetabolismandunravelsitsprotectiveeffectagainstsevereintoxicationinresponsetobingedrinking.
Comparisonofsyntheticmediumandwastewaterusedasdilutionmediumtodesignscalablemicrobialanodes:applicationtofoodwastetreatment.
Blanchet,E.,Desmond,E.,Erable,B.,Bridier,A.,Bouchez,T.&Bergel,A.(2015).BioresourceTechnology,185,106-115.
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Theobjectivewastoreplacesyntheticmediumbywastewaterasastrategytodesignlow-costscalablebioanodes.Theadditionofactivatedsludgewasnecessarytoformprimarybioanodesthatwerethenusedastheinoculumtoformthesecondarybioanodes.Bioanodesformedinsyntheticmediumwithacetate10 mMprovidedcurrentdensitiesof21.9 ± 2.1 A/m2,whilebioanodesformedinwastewatergave10.3 ± 0.1 A/m2.Thedifferencewasexplainedintermsofbiofilmstructure,electrochemicalkineticsandredoxchargecontentofthebiofilms.Inbothmedia,currentdensitieswerestraightforwardlycorrelatedwiththebiofilmenrichmentinGeobacteraceaebut,insidethisfamily,GeobactersulfurreducensandanunculturedGeobactersp.weredominantinthesyntheticmedium,whilegrowthofanotherGeobactersp.wasfavouredinwastewater.Finally,theprimary/secondaryproceduresucceededindesigningbioanodestotreatfoodwastesbyusingwastewaterasdilutionmedium,withcurrentdensitiesof7 ± 1.1 A/m2.
Influenceoftheelectrodesizeonmicrobialanodeperformance.
Oliot,M.,Chong,P.,Erable,B.&Bergel,A.(2017).ChemicalEngineeringJournal,327,218-227.
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Theperformanceofmicrobialfuelcellsandotherrelatedmicrobialelectrochemicalprocessesisseentodeteriorateseverelywhentheyarescaledup.Thiscrucialproblemisaddressedherebycomparingthekineticsofmicrobialanodeswithprojectedsurfaceareasof9and50cm2underwell-controlledelectrochemicalconditions.Themicrobialanodekineticswerecharacterizedbylowscanratevoltammetry.The9-cm2anodesshowedNernstianbehaviour,whilethe50-cm2anodesshowedsignificantlylowerperformance.Thedistributionoftheelectrostaticpotentialintheexperimentalset-upwasmodellednumerically.Themodelpredictedthegeneraltrendofthevoltammetrycurvesrecordedwiththe50-cm2anodeswell,showingthatpartoftheperformancedeteriorationwasduetoohmicdropandtonon-uniformityofthelocalpotentialovertheanodesurface.Furthermore,thebiofilmpresentedslightlydifferentelectrochemicalcharacteristicswhengrownonthe9-cm2or50-cm2anodes,andthedifferenceinlocalpotentialoverthe50-cm2anodesinducedspatialheterogeneityinbiofilmdevelopment.Theeffectoflocalpotentialonbiofilmcharacteristicswasanadditionalcauseofthelowerperformanceobtainedwiththe50-cm2anodes.Inthecurrentstateoftheart,thesoundestwaytodesignlarge-sizedmicrobialanodesistoadoptthedualmainaimofminimizingtheohmicdropwhilekeepingthemostuniformpossiblepotentialovertheelectrodesurface.Modellingpotentialdistributioninsidethereactorshouldmakeanessentialcontributiontothis.
AnalyserformatUV-methodforthedeterminationofAceticAcid
infoodstuffs,beveragesandothermaterials
Principle:
(acetatekinase)
(1)Aceticacid+ATP→acetyl-phosphate+ADP
(pyruvatekinase)
(2)ADP+PEP→ATP+pyruvate
(D-lactatedehydrogenase)
(3)Pyruvate+NADH+H+→D-lacticacid+NAD+
Kitsize: 550assays
Method: Spectrophotometricat340nm
Reactiontime: ~10min
Detectionlimit: 10mg/L(recommendedassayformat)
Applicationexamples:
Wine,beer,fruitandfruitjuices,softdrinks,vinegar,vegetables,
pickles,dairyproducts(e.g.cheese),meat,fish,bread,bakeryproducts
(andbakingagents),ketchup,soysauce,mayonnaise,dressings,
paper(andcardboard),tea,pharmaceuticals(e.g.infusionsolutions),
feedandothermaterials(e.g.biologicalcultures,samples,etc.)
Methodrecognition: Improvedmethod
Advantages
- Verystablereagentwhenpreparedforauto-analyserapplications(>7daysat4°C)
- PVPincorporatedtopreventtannininhibition
- Linearcalibration(R2~0.9995)upto30μg/mLofaceticacidinfinalreactionsolution
- ValidatedbytheUniversityofWine,SuzelaRousse,France
- Veryrapidreaction
- Verycompetitiveprice(costpermLofreagent)
- Allreagentsstablefor>2years
- Extendedcofactorsstability
品牌介绍
Megazyme品牌产品简介
来源：作者：人气：2149发表时间：2016-05-19 10:59:00【大 中 小】
Megazyme是一家全球性公司，专注于开发和提供用于饮料、谷物、乳制品、食品、饲料、发酵、生物燃料和葡萄酒产业用的分析试剂、酶和检测试剂盒。Megazyme的许多检测试剂盒产品已经为众多官方科学协会（包括AOAC, AACC , RACI, EBC和ICC等），经过严格的审核，批准认证为官方标准方法，确保以准确、可靠、定量和易于使用的测试方法，满足客户的质量诉求。
Megazyme的主要产品线包括：
◆ 检测试剂盒
◆ 酶
◆ 酶底物
◆ 碳水化合物
◆ 化学品/仪器
官网地址：http://www.megazyme.com
检测试剂盒特色产品：
货号
中文品名
用途
K-ACETAF
乙酸[AF法]检测试剂盒
酶法定量分析乙酸最广泛使用的方法
K-ACHDF
可吸收糖/膳食纤维检测试剂盒
酒精沉淀法测定膳食纤维
K-AMIAR
氨快速检测试剂盒
用于包括葡萄汁、葡萄酒以及其它食品饮料样品中氨含量的快速检测分析。
K-AMYL
直链淀粉/支链淀粉检测试剂盒
谷物淀粉和而粉中直链淀粉/支链淀粉比例和含量检测
K-ARAB
阿拉伯聚糖检测试剂盒
果汁浓缩液中阿拉伯聚糖的检测
K-ASNAM
L-天冬酰胺/L-谷氨酰胺和氨快速检测试剂盒
用于食品工业中丙烯酰胺前体、细胞培养基、以及上清液组分中、L-天冬酰胺，谷氨酰胺和氨的检测分析
K-ASPTM
阿斯巴甜检测试剂盒
专业用于测定饮料和食品中阿斯巴甜含量，操作简单
K-BETA3
β-淀粉酶检测试剂盒
适用于麦芽粉中β-淀粉酶的测定
K-BGLU
混合键β-葡聚糖检测试剂盒
测定谷物、荞麦粉、麦汁、啤酒及其它食品中混合键β-葡聚糖(1,3:1,4-β-D-葡聚糖)的含量
K-CERA
α-淀粉酶检测试剂盒
谷物和发酵液（真菌和细菌）中α-淀粉酶的分析测定
K-CITR
柠檬酸检测试剂盒
快速、可靠地检测食品、饮料和其它物料中柠檬酸（柠檬酸盐）含量
K-DLATE
乳酸快速检测试剂盒
快速、特异性检测饮料、肉类、奶制品和其它食品中L-乳酸和D-乳酸(乳酸盐）含量
K-EBHLG
酵母β-葡聚糖酶检测试剂盒
用于测量和分析酵母中1,3:1,6?-β-葡聚糖，也可以检测1,3-葡聚糖
K-ETSULPH
总亚硫酸检测试剂盒
测定葡萄酒、饮料、食品和其他物料中总亚硫酸含量（按二氧化硫计）的一种简单，高效，可靠的酶法检测方法
K-FRGLMQ
D-果糖/D-葡萄糖[MegaQuant法]检测试剂盒
适用于使用megaquant?色度计（505nm下）测定葡萄、葡萄汁和葡萄酒中D-果糖和D-葡萄糖的含量。
K-FRUC
果聚糖检测试剂盒
含有淀粉、蔗糖和其他糖类的植物提取物和食品中果聚糖的含量测定。
K-FRUGL
D-果糖/D-葡萄糖检测试剂盒
对植物和食品中果糖或葡萄糖含量的酶法紫外分光测定。
K-GALM
半乳甘露聚糖检测试剂盒
食品和植物产品中半乳甘露聚糖的含量检测
K-GLUC
D-葡萄糖[GOPOD]检测试剂盒
谷物提取物中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUHK
D-葡萄糖[HK]检测试剂盒
植物和食品中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUM
葡甘聚糖检测试剂盒
植物和食品中葡甘聚糖的含量测定。
K-INTDF
总膳食纤维检测试剂盒
总膳食纤维特定检测和分析
K-LACGAR
乳糖/D-半乳糖快速检测试剂盒
用于快速检测食品和植物产品中乳糖、D-半乳糖和L-阿拉伯糖
K-LACSU
乳糖/蔗糖/D-葡萄糖检测试剂盒
混合面粉和其它物料中蔗糖、乳糖和D-葡萄糖的测定
K-LACTUL
乳果糖检测试剂盒
特异性、快速和灵敏测量奶基样品中乳果糖含量
K-MANGL
D-甘露糖/D-果糖/D-葡萄糖检测试剂盒
适合测定植物产品和多糖酸性水解产物中D-甘露糖含量
K-MASUG
麦芽糖/蔗糖/D-葡萄糖检测试剂盒
在植物和食品中麦芽糖，蔗糖和葡萄糖的含量检测
K-PECID
胶质识别检测试剂盒
食品配料中果胶的鉴别
K-PHYT
植酸(总磷)检测试剂盒
食品和饲料样品植酸/总磷含量测量的简便方法。不需要通过阴离子交换色谱对植酸纯化，适合于大量样本分析
K-PYRUV
丙酮酸检测试剂盒
在啤酒、葡萄酒、果汁、食品和体液中丙酮酸分析
K-RAFGA
棉子糖/D-半乳糖检测试剂盒
快速测量植物材料和食品中棉子糖和半乳糖含量
K-RAFGL
棉子糖/蔗糖/D-半乳糖检测试剂盒
分析种子和种子粉中D-葡萄糖、蔗糖、棉子糖、水苏糖和毛蕊花糖含量。通过将棉子糖、水苏糖和毛蕊花糖酶解D-葡萄糖、D-果糖和半乳糖，从而测定葡萄糖含量来确定
K-SDAM
淀粉损伤检测试剂盒
谷物面粉中淀粉损伤的检测和分析
K-SUCGL
蔗糖/D-葡萄糖检测试剂盒
饮料、果汁、蜂蜜和食品中蔗糖和葡萄糖的分析
K-SUFRG
蔗糖/D-果糖/D-葡萄糖检测试剂盒
适用于植物和食品中蔗糖、D-葡萄糖和D-果糖的测定
K-TDFR
总膳食纤维检测试剂盒
总膳食纤维检测
K-TREH
海藻糖检测试剂盒
快速、可靠地检测食品、饮料和其它物料中海藻糖含量
K-URAMR
尿素/氨快速检测试剂盒
适用于水、饮料、乳制品和食品中尿素和氨的快速测定
K-URONIC
D-葡萄糖醛酸/D-半乳糖醛酸检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中六元糖醛酸含量（D-葡萄糖醛酸和D-半乳糖醛酸）
K-XYLOSE
D-木糖检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中D-木糖含量
K-YBGL
Beta葡聚糖[酵母和蘑菇]检测试剂盒
检测酵母和蘑菇制品中1,3:1,6-beta-葡聚糖和α-葡聚糖含量
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