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Megazyme/Acetic Acid GK Assay Kit (Analyser Format) /K-ACETGK/110 mL of prepared reagent (e.g. 500 assays of 0.22 m
TheAceticAcidGKformattestkitisforusewithauto-analysersandissuitableforthespecificmeasurementandanalysisofaceticacid(acetate)especiallyinwines,fruitjuices,beveragesandfoodproducts.
AspartofMegazyme’soverallcommitmenttoprovidingthehighestqualityproducts,wehavedevelopedthisaceticacidkitthatprovidesaspecificandrapidassayforusewithauto-analysers.ThekitassayisbasedontheconversionofNAD+toNADHandthereforeprovidesapositivereaction(increaseinabsorbance)whichoffersamorerobustassay.
Thereagents,assupplied,arestableforaminimumof2yearsandthepreparedreagentsarestableforaminimumof1week(on-boardstABIlity).Inaddition,thepreparedreagentscanbestoredfrozenforlongertermstability(seeK-ACETGKBookletformoredetails).
Grapeandwineanalysis:Oenologiststoexploitadvancedtestkits.
Megazyme“advanced”winetestkitsgeneralcharacteristicsandvalidation.
AnalyserformatUV-methodforthedeterminationofAceticAcid
infoodstuffs,beveragesandothermaterials
Principle:
(acetatekinase)
(1)Aceticacid+ATP→acetyl-phosphate+ADP
(PTA)
(2)Acetyl-phosphate+CoA→acetyl-CoA+Pi
(ADP-GK)
(3)ADP+D-glucose→glucose-6-phosphate+AMP
(G6P-DH)
(4)Glucose-6-phosphate+NAD+→6-phosphogluconate+NADH +H+
Kitsize: 500assays
Method: Spectrophotometricat340nm
Reactiontime: 8minat25°Cor5minat37°C
Detectionlimit: ~10mg/L
Applicationexamples:
Wine,beer,fruitandfruitjuices,softdrinks,vinegar,vegetables,
pickles,dairyproducts(e.g.cheese),meat,fish,bread,bakeryproducts(andbakingagents),ketchup,soysauce,mayonnaise,dressings,paper
(andcardboard),tea,pharmaceuticals(e.g.infusionsolutions),feed,
andothermaterials(e.g.biologicalcultures,samples,etc.)
Methodrecognition: Improvedmethod
Advantages
- Excellentreagentstability
- >7daysat4°Cor>2yearsat-20°Cwhenpreparedforauto-analyserapplications
- >2yearsassupplied
- Veryrapidreaction(~5minat37°C)
- Linearcalibration(R2~0.997upto1.8g/Lsample)