当前位置：
首页
>
产品中心 >
Other_kits >
Megazyme/Acetic Acid Assay Kit (Acetate Kinase Manual Format) /K-ACETRM/72 assays (manual) / 720 assays (microplate)
Megazyme/Acetic Acid Assay Kit (Acetate Kinase Manual Format) /K-ACETRM/72 assays (manual) / 720 assays (microplate)
商品编号：
K-ACETRM
品牌：
Megazyme INC
市场价：
¥3520.00
美元价：
2112.00
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
TheAceticAcid(AcetateKinaseManualFormat)testkitissuitableforthemeasurementandanalysisofaceticacidinfoodandbeverages.
Thisrapidandreliablemanualaceticacidkitissimpletoperform(onlytwoabsorbancereADIngsrequired),andbecauseatrueend-pointismeasured,doesnotinvolvecomplicatedcalculationslikeotherkits.Thisproductisverystablebothduringstorageanduse(>2years),hasextendedlinearity(comparedtoACSbasedkits),containsPVPtopreventtannininhibition,andisperformedatarelativelylowpH(7.4),thusminimisingesterhydrolysisrelatedinterference.Thismethodissuitableforthemeasurementofaceticacid/acetateinfoods,beveragesandothermaterials.
Suitableformanualandmicroplateformats.
Grapeandwineanalysis:Oenologiststoexploitadvancedtestkits.
Charnock,S.C.&McCleary,B.V.(2005).RevuedesEnology,117,1-5.
LinktoArticle
ReadAbstract
Itiswithoutdoubtthattestingplaysapivotalrolethroughoutthewholeofthevinificationprocess.ToproducethebestpossIBLequalitywineandtominimiseprocessproblemssuchas“stuck”fermentationortroublesomeinfections,itisnowrecognisedthatifpossibletestingshouldbeginpriortoharvestingofthegrapesandcontinuethroughtobottling.Traditionalmethodsofwineanalysisareoftenexpensive,timeconsuming,requireeitherelaborateequipmentorspecialistexpertiseandfrequentlylackaccuracy.However,enzymaticbio-analysisenablestheaccuratemeasurementofthevastmajorityofanalytesofinteresttothewinemaker,usingjustonepieceofapparatus,thespectrophotometer(seepreviousissueNo.116foradetailedtechnicalreview).Grapejuiceandwineareamenabletoenzymatictestingasbeingliquidstheyarehomogenous,easytomanipulate,andcangenerallybeanalysedwithoutanysamplepreparation.
Megazyme“advanced”winetestkitsgeneralcharacteristicsandvalidation.
Charnock,S.J.,McCleary,B.V.,Daverede,C.&Gallant,P.(2006).ReveuedesOenologues,120,1-5.
LinktoArticle
ReadAbstract
ManyoftheenzymatictestkitsareofficialmethodsofprestigiousorganisationssuchastheAssociationofOfficialAnalyticalChemicals(AOAC)andtheAmericanAssociationofCerealChemists(AACC)inresponsetotheinterestfromoenologists.Megazymedecidedtouseitslonghistoryofenzymaticbio-analysistomakeasignificantcontributiontothewineindustry,bythedevelopmentofarangeofadvancedenzymatictestkits.Thistaskhasnowbeensuccessfullycompletedthroughthestrategicandcomprehensiveprocessofidentifyinglimitationsofexistingenzymaticbio-analysistestkitswheretheyoccurred,andthenusingadvancedtechniques,suchasmolecularBIOLOGy(photo1),torapidlyovercomethem.Noveltestkitshavealsobeendevelopedforanalytesofemerginginteresttotheoenologist,suchasyeastavailablenitrogen(YAN;seepages2-3ofissue117article),orwherepreviouslyenzymesweresimplyeithernotavailable,orweretooexpensivetoemploy,suchasforD-mannitolanalysis.
ThemetabolicpotentialofEscherichiacoliBL21indefinedandrichmedium.
Li,Z.,Nimtz,M.&Rinas,U.(2014).MicrobialCellFactories, 13(1),45.
LinktoArticle
ReadAbstract
Background:Theproteomereflectstheavailablecellularmachinerytodealwithnutrientsandenvironmentalchallenges.ThemostcommonE.colistrainBL21growingindifferent,commonlyemployedmediawasevaluatedusingadetailedquantitativeproteomeanalysis.Results:ThepresenceofpreformedbiomassprecursormoleculesinrichmediasuchasLuriaBertanisupportedrapidgrowthconcomitanttoacetateformationandapparentlyunbalancedabundancesofcentralmetabolicpathwayenzymes,e.g.highlevelsoflowerglycolyticpathwayenzymesaswellaspyruvatedehydrogenase,andlowlevelsofTCAcycleandhighlevelsoftheacetateformingenzymesPtaandAckA.Theproteomeofcellsgrowingexponentiallyinglucose-supplementedmineralsaltmediumwasdominatedbyenzymesofaminoacidsynthesispathways,containedmorebalancedabundancesofcentralmetabolicpathwayenzymes,andalowerportionofribosomalandothertranslationalproteins.Entryintostationaryphaseledtoareconstructionofthebacterialproteomebyincreasinge.g.theportionofproteinsrequiredforscavengingrarenutrientsandgeneralcellprotection.Thisproteomicreconstructionduringentryintostationaryphasewasmorenoticeableincellsgrowinginrichmediumastheyhaveagreaterreservoirofrecyclableproteinsfromthetranslationalmachinery.Conclusions:TheproteomiccomparisonofcellsgrowingexponentiallyindifferentmediareflectedtheantagoNISTicandcompetitiveregulationofcentralmetabolicpathwaysthroughtheglobaltranscriptionalregulatorsCra,Crp,andArcA.Forexample,theproteomeofcellsgrowingexponentiallyinrichmediumwasconsistentwithadominatingroleofphosphorylatedArcAmostlikelyaresultfromlimitationsinreoxidizingreducedquinonesintherespiratorychainunderthesegrowthconditions.Theproteomicalterationsofexponentiallygrowingcellsintostationaryphasecellswereconsistentwithstringent-likeandstationaryphaseresponsesandadominatingcontrolthroughDksA-ppGppandRpoS.
Comparativetranscriptomicprofileanalysisoffed-batchculturesexpressingdifferentrecombinantproteinsinEscherichiacoli.
Sharma,A.K.,Mahalik,S.,Ghosh,C.,Singh,A.B.&Mukherjee,K.J.(2011).AMBExpress,1(1),33.
LinktoArticle
ReadAbstract
Thereisaneedtoelucidatetheproductspecificfeaturesofthemetabolicstressresponseofthehostcelltotheinductionofrecombinantproteinsynthesis.Forthis,themethodofchoiceistranscriptomicprofilingwhichprovidesabetterinsightintothechangestakingplaceincomplexglobalmetabolicnetworks.Thetranscriptomicprofilesofthreefed-batchculturesexpressingdifferentproteinsviz.recombinanthumaninterferon-beta(rhIFN-β),XylanaseandGreenFluorescenceProtein(GFP)werecomparedpostinduction.Weobservedadepressioninthenutrientuptakeandutilizationpathways,whichwascommonforallthethreeexpressedproteins.ThusglyceroltransportersandgenesinvolvedinATPsynthesisaswellasaerobicrespirationwereseverelydown-regulated.Ontheotherhandtheaminoaciduptakeandbiosynthesisgenesweresignificantlyrepressedonlywhensolubleproteinswereexpressedunderdifferentpromoters,butnotwhentheproductwasexpressedasaninclusionbody(IB).HighlevelexpressionundertheT7promoter(rhIFN-βandxylanase)triggeredthecellulardegradationmachineryliketheosmoprotectants,proteasesandmRNAdegradationgeneswhichwerehighlyup-regulated,whilethistrendwasnottruewithGFPexpressionunderthecomparativelyweakerarapromoter.Thedesignofabetterhostplatformforrecombinantproteinproductionthusneedstotakeintoaccountthespecificnatureofthecellularresponsetoproteinexpression.
OrganiccarbonsupplementationofsterilizedmunicipalwastewaterisessentialforheterotrophicgrowthandremovingammoniumbythemicroalgaChlorellaVulgaris.
Perez‐Garcia,O.,Bashan,Y.&EstherPuente,M.(2011).JournalofPhycology,47(1),190-199.
LinktoArticle
ReadAbstract
HeterotrophicgrowthofthemicroalgaChlorellavulgarisBeij.insyntheticaswellassterilizedmunicipalwastewaterofanonindustrializedcitywasmeasured.Thecitywastewatercontainedhighlevelsofammoniumandnitrate,mediumlevelsofphosphate,andlowlevelsofnitriteandorganicmoleculesandcouldnotsupportheterotrophicgrowthofC.vulgaris.Evaluationof11knowncarbonsourcesforthismicroalgathatwereaddedtostandardsyntheticwastewatercontainingthesamelevelsofnitrogenandphosphorusasthemunicipalwastewaterrevealedthatthebestcarbonsourcesforheterotrophicgrowthwereNa-acetateandD-glucose.Theseprovidedthehighestgrowthratesandthelargestremovalofammonium.Growthincreasedwithconcentrationofthesupplementtoanoptimumat0.12MNa-acetate.Thiscarbonsourcewasconsumedcompletelywithin10dofincubation.HigherconcentrationsinhibitedthegrowthofC.vulgaris.Themicroalgalpopulationsunderheterotrophicgrowthconditionswereonelevelofmagnitudehigherthanthatunderautotrophicgrowthconditionsthatservedasacomparison.Nogrowthoccurredinthedarkintheabsenceofacarbonsource.Na-acetatewassuperiortoD-glucose.Inmunicipalwastewater,whenNa-acetateorD-glucosewasadded,C.vulgarissignificantlyenhancedammoniumremovalunderheterotrophicconditions,anditscapacitywasequaltoammoniumremovalunderautotrophicgrowthconditions.ThisstudyshowedthatsterilizedwastewatercanbetreatedbyC.vulgarisunderheterotrophicconditionsifsupplementedwiththeappropriateorganiccarbonsourceforthemicroalgae.
Large-scalemetabolomeanalysisandquantitativeintegrationwithgenomicsandproteomicsdatainMycoplasmapneumonia.
Maier,T.,Marcos,J.,Wodke,J.A.H.,Paetzold,B.,Liebeke,M.,Gutiérrez-Gallego,R.&Serrano,L.(2013).MolecularBioSystems,9(7),1743-1755.
LinktoArticle
ReadAbstract
Systemsmetabolomics,theidentificationandquantificationofcellularmetabolitesandtheirintegrationwithgenomicsandproteomicsdata,promisesvaluablefunctionalinsightsintocellularbiology.However,technicalconstraints,samplecomplexityissuesandthelackofsuitablecomplementaryquantitativedatasetspreventedaccomplishingsuchstudiesinthepast.Here,wepresentanintegrativemetabolomicsstudyofthegenome-reducedbacteriumMycoplasmapneumoniae.Weexperimentallyanalyseditsmetabolomeusingacross-platformapproach.Weexplainintracellularmetabolitehomeostasisbyquantitativelyintegratingourresultswiththecellularinventoryofproteins,DNAandothermacromolecules,aswellaswithavailablebuildingblocksfromthegrowthmedium.Wecalculatedinvivocatalyticparametersofglycolyticenzymes,makinguseofmeasuredreactionvelocities,aswellasenzymeandmetabolitepoolsizes.Aquantitative,inter-speciescomparisonofabsoluteandrelativemetaboliteabundancesindicatedthatmetabolicpathwaysareregulatedasfunctionalunits,therebysimplifyingadaptiveresponses.Ouranalysisdemonstratesthepotentialfornewscientificinsightbyintegratingdifferenttypesoflarge-scaleexperimentaldatafromasinglebiologicalsource.
Declineinmammarytranslationalcapacityduringintravenousglucoseinfusionintolactatingdairycows.
Curtis,R.V.,Kim,J.J.M.,Bajramaj,D.L.,Doelman,J.,Osborne,V.R.&Cant,J.P.(2014).JournalofDairyScience,97(1),430-438.
LinktoArticle
ReadAbstract
Theobjectiveofthisstudywastodetermineeffectsofglucoseonmilkproteinyieldandmammarymammaliantargetofrapamycin(mTOR)activityindairycattleinearlylactation.Eightmultiparouscowsat73±8dinmilkwererandomlyassignedto2treatmentsinacrossoverdesignfortwo6-dperiods.Treatmentswerejugularinfusionofeithersaline(Sal)or896g/dglucose(Glc).Allcowswerefedatotalmixedrationwith42%neutraldetergentfiber,hadfreeaccesstowater,andweremilkedtwiceaday.Withineachperiod,bloodsamplesweretaken(d5)andmammarytissuewascollectedbybiopsy(d6)fromeachhindquarterforWesternblotanalysis.InadditiontoSalandGlctreatments,ond6,rapamycindissolvedin50%dimethylsulfoxidewasadministeredviatheteatcanalsintotheleftquarters,withacontrolsolutionadministeredintotherightquarters.RapamycinhadnoeffectonmilkproteinyieldsorphosphorylationstateofmTORsignalingproteins.InfusionsofGlcsignificantlyincreasedmilkyieldbutonlytendedtoincreasemilkproteinyields.MilkfattendedtobedecreasedincowsinfusedwithGlc,whereaslactoseyieldsweresignificantlyincreased.Glucoseinfusiondidnotincreaseplasmaglucoselevels,butinsulinandnonessentialAAconcentrationsincreasedby21and16%,respectively,branched-chainAAconcentrationsdecreased24%,andessentialAAconcentrationstendedtodecreaseby14%.InfusionofGlcsignificantlydecreasedabundancesofbothphosphorylatedandtotalribosomalS6kinase1(S6K1)inmammarytissueby27and11%,respectively.Abundanceofphosphorylatedeukaryoticinitiationfactor4E-bindingprotein1(4EBP1)decreasedsignificantlyby25%,whereastotal4EBP1exhibitedatendencytodecreaseby16%.WeconcludethatthemTORsignalingpathwayisnottheonlyregulatorofmilkproteinsynthesis.DecreasesinessentialAAconcentrationsinplasmasuggestthatproteinsynthesiswasstimulatedinnonmammarytissuesofthebody,presumablyskeletalmuscle.
AnewfamilyofcarbohydrateesterasesisrepresentedbyaGDSLhydrolase/acetylxylanesterasefromGeobacillusstearoThermophilus.
Alalouf,O.,Balazs,Y.,Volkinshtein,M.,Grimpel,Y.,Shoham,G.&Shoham,Y.(2011).JournalofBiologicalChemistry,286(49),41993-42001.
LinktoArticle
ReadAbstract
Acetylxylanesteraseshydrolyzetheesterlinkagesofacetylgroupsatpositions2and/or3ofthexylosemoietiesinxylanandplayanimportantroleinenhancingtheaccessibilityofxylanasestothexylanbackbone.ThehemicellulolyticsystemofthethermophilicbacteriumGeobacillusstearothermophilusT-6comprisesaputativeacetylxylanesterasegene,axe2.ThegeneproductbelongstotheGDSLhydrolasefamilyanddoesnotsharesequencehomologywithanyofthecarbohydrateesterasesintheCAZyDatabase.Theaxe2geneisinducedbyxylose,andthepurifiedgeneproductcompletelydeacetylatesxylobioseperacetate(fullyacetylated)andhydrolyzesthesyntheticsubstrates2-naphthylacetate,4-nitrophenylacetate,4-methylumbelliferylacetate,andphenylacetate.ThepHprofilesforKcatandKcat/Kmsuggesttheexistenceoftwoionizablegroupsaffectingthebindingofthesubstratetotheenzyme.UsingNMRspectroscopy,theregioselectivityofAxe2wasdirectlydeterminedwiththeaidofone-dimensionalselectivetotalcorrelationspectroscopy.Methyl2,3,4-tri-O-acetyl-β-D-xylopyranosidewasrapidlydeacetylatedatposition2oratpositions3and4togiveeitherdiacetylormonoacetylintermediates,respectively;methyl2,3,4,6-tetra-O-acetyl-β-D-glucopyranosidewasinitiallydeacetylatedatposition6.Inbothcases,thecompletehydrolysisoftheintermediatesoccurredatamuchslowerrate,suggestingthatthepreferredsubstrateistheperacetatesugarform.Site-directedmutagenesisofSer-15,His-194,andAsp-191resultedincompleteinactivationoftheenzyme,consistentwiththeirroleasthecatalytictriad.Overall,ourresultsshowthatAxe2isaserineacetylxylanesteraserepresentinganewcarbohydrateesterasefamily.
Microbialcarbohydrateesterasesdeacetylatingplantpolysaccharides.
Biely,P.(2012).BiotechnologyAdvances,30(6),1575-1588.
LinktoArticle
ReadAbstract
Severalplantpolysaccharidesarepartiallyesterifiedwithaceticacid.Oneoftherolesofthismodificationisprotectionofplantcellwallsagainstinvadingmicroorganisms.Acetylationofglycosylresiduesofpolysaccharidespreventshydrolysisoftheirglycosidiclinkagesbythecorrespondingglycosidehydrolases.Inthiswaytheacetylationalsorepresentsanobstacleofenzymaticsaccharificationofplanthemicellulosestofermentablesugarswhichappearstobeahottopicofcurrentresearch.Wecaneliminatethisobstaclebyalkalineextractionorpretreatmentleadingtosaponificationofesterlinkages.However,thistaskhasbeenaccomplishedinadifferentwayinthenature.Theacetylgroupsbecametargetsofmicrobialcarbohydrateesterasesthatevolvedtoovercomethecomplexityoftheplantcellwallsandthatcooperatewithglycosidehydrolasesinplantpolysaccharidedegradation.Thisarticleconcentratesonenzymesdeacetylatingplanthemicellulosesexcludingpectin.Theyarecurrentlygroupedinatleast8families,specificallyinCEfamilies1–7and16,originallyassignedasacetylxylanesterases,theenzymesactingonhardwoodacetylglucuronoxylananditsfragmentsgeneratedbyendo-β-1,4-xylanases.Thereareesterasesdeacetylatingsoftwoodgalactoglucomannan,buttheyhavenotbeenclassifiedyet.TheenzymespresentinCEfamilies1–7differinstructureandsubstrateandpositionalspecificity.Therearefamiliesbehavingasendo-typeandexo-typedeacetylates,i.e.esterasesdeacetylatinginternalsugarresiduesofpartiallyacetylatedpolysaccharidesandalsoesterasesdeacetylatingnon-reducingendsugarresiduesinoligosaccharides.Withoneexception,theenzymesofallmentionedCEfamiliesbelongtoserinetypeesterases.CEfamily4harborsenzymesthataremetal-dependentasparticesterases.Three-dimensionalstructureshavebeensolvedformembersofthefirstsevenCEfamilies,however,thereisstillinsufficientknowledgeabouttheirsubstratespecificityandrealphysiologicalrole.CurrentknowledgeoncatalyticpropertiesoftheselectedfamiliesofCEsissummarizedinthisreview.Someofthefamiliesareemergingalsoasnewbiocatalystsforregioselectiveacylationanddeacylationofcarbohydrates.
AssayforpeptidoglycanO-acetyltransferase:Apotentialnewantibacterialtarget.
Moynihan,P.J.&Clarke,A.J.(2013).AnalyticalBiochemistry,439(2),73-79.
LinktoArticle
ReadAbstract
TheO-acetylationofpeptidoglycanoccursattheC-6hydroxylgroupofmuramoylresiduesinmanyhumanpathogens,bothgrampositiveandgramnegative,suchasStaphylococcusaureusandspeciesofCampylobacter,Helicobacter,Neisseria,andBacillus,includingBacillusanthracis.TheprocessisamaturationeventbeingcatalyzedeitherbyintegralmembraneO-acetylpeptidoglycantransferase(Oat)ofgram-positivebacteriaorbyatwo-componentpeptidoglycanO-acetyltransferasesystem(PatA/PatB)ingram-negativecells.Here,wedescribethedevelopmentofthefirstinvitroassayforanypeptidoglycanO-acetyltransferaseusingPatBfromNeisseriagonorrhoeaeasthemodelenzyme.Thisassayisbasedontheuseofchromogenicp-nitrophenylacetateasthedonorsubstrateandchitooligosaccharidesasmodelacceptorsubstratesinplaceofpeptidoglycan.TheidentityoftheO-acetylatedchitooligosaccharideswasconfirmedbymatrix-assistedlaserdesorption/ionizationtime-of-flightmassspectrometry.Ratesoftransacetylationsweredeterminedspectrophotometricallybymonitoringp-nitrophenolreleaseafteraccountingforbothspontaneousandenzyme-catalyzedhydrolysisoftheacetatedonor.Conditionswereestablishedforuseoftheassayinmicrotiterplateformat,anditsapplicABIlitywasdemonstratedbydeterminingthefirstMichaelis–MentenkineticparametersforPatB.Theassayisreadilyamenableforapplicationinthehigh-throughputscreeningforpotentialinhibitorsofpeptidoglycanO-acetyltransferasesthatmayprovetobeleadsfornovelclassesofantibiotics.
Discoveryofdiversityinxylanbiosyntheticgenesbytranscriptionalprofilingofaheteroxylancontainingmucilaginoustissue.
Jensen,J.K.,Johnson,N.&Wilkerson,C.G.(2013).FrontiersinPlantScience,4(183).
LinktoArticle
ReadAbstract
Theexactbiochemicalstepsofxylanbackbonesynthesisremainelusive.InArabidopsis,threenon-redundantgenesfromtwoglycosyltransferase(GT)families,IRX9andIRX14fromGT43andIRX10fromGT47,arecandidatesforformingthexylanbackbone.Inotherplants,evidenceexiststhatdifferenttissuesexpressthesethreegenesatwidelydifferentlevels,whichsuggeststhatdiversityinthemakeupofthexylansynthasecomplexexists.Recentlywehaveprofiledthetranscriptspresentinthedevelopingmucilaginoustissueofpsyllium(PlantagoovataForsk).ThistissuewasfoundtohavehighexpressionlevelsofanIRX10homolog,butverylowlevelsofthetwoGT43familymembers.ThiscontrastswithrecentwheatendospermtissueprofilingthatfoundarelativelyhighabundanceoftheGT43familymembers.Wehaveperformedanin-depthanalysisofallGTsgenesexpressedinfourdevelopmentalstagesofthepsylliummucilagenouslayerandinasinglestageofthepsylliumstemusingRNA-Seq.ThisanalysisrevealedseveralIRX10homologs,anexpansioninGT61(homologsofAt3g18170/At3g18180),andseveralGTsfromotherGTfamiliesthatarehighlyabundantandspecificallyexpressedinthemucilaginoustissue.OurcurrenthypothesisisthatthefourIRX10genespresentinthemucilagenoustissueshaveevolvedtofunctionwithouttheGT43genes.ThesefourgenesrepresentsomeofthemostdivergentIRX10genesidentifiedtodate.Conversely,thosepresentinthepsylliumstemareverysimilartothoseinothereudicots.Thissuggeststhesegenesareunderselectivepressure,likelyduetothesynthesisofthevariousxylanstructurespresentinmucilagethathasadifferentbiochemicalrolethanthatpresentinsecondarywalls.ThenumerousGT61familymembersalsoshowawidesequencediversityandmayberesponsibleforthelargernumberofsidechainstructurespresentinthepsylliummucilage.
Structuralchangesandcellularlocalizationofresuscitation-promotingfactorinenvironmentalisolatesofMicrococcusluteus.
Koltunov,V.,Greenblatt,C.L.,Goncharenko,A.V.,Demina,G.R.,Klein,B.Y.,Young,M.&Kaprelyants,A.S.(2010).MicrobialEcology,59(2),296-310.
LinktoArticle
ReadAbstract
Dormancyamongnonsporulatingactinobacteriaisnowawidelyacceptedphenomenon.InMicrococcusluteus,theresuscitationofdormantcellsiscausedbyasmallsecretedprotein(resuscitation-promotingfactor,orRpf)thatisfoundin“spentculturemedium.”RpfisencodedbyasingleessentialgeneinM.luteus.HomologsofRpfarewidespreadamongthehighG+CGram-positivebacteria,includingmycobacteriaandstreptomycetes,andmostorganismsmakeseveralfunctionallyredundantproteins.M.luteusRpfcomprisesalysozyme-likedomainthatisnecessaryandsufficientforactivityconnectedthroughashortlinkerregiontoaLysMmotif,whichispresentinanumberofcell-wall-associatedenzymes.Muralyticactivityisresponsibleforresuscitation.Inthisreport,wecharacterizedanumberofenvironmentalisolatesofM.luteus,includingseveralrecoveredfromamber.Therewassubstantialvariationinthepredictedrpfgeneproduct.Whilethelysozyme-likeandLysMdomainsshowedlittlevariation,thelinkerregionwaselongatedfromtenaminoacidresiduesinthelaboratorystrainstoasmanyas120residuesinoneisolate.ThegenesencodingtheseRpfproteinshavebeencharacterized,andapossiblerolefortheRpflinkerinenvironmentaladaptationisproposed.Theenvironmentalisolatesshowenhancedresistancetolysozymeascomparedwiththelaboratorystrainsandthiscorrelateswithincreasedpeptidoglycanacetylation.Instrainsthatmakeaproteinwithanelongatedlinker,Rpfwasboundtothecellwall,ratherthanbeingreleasedtothegrowthmedium,asoccursinreferencestrains.Thisrpfgenewasintroducedintoalysozyme-sensitivereferencestrain.Bothrpfgeneswereexpressedintransformantswhichshowedaslightbutstatisticallysignificantincreaseinlysozymeresistance.
ManualformatUV-methodforthedeterminationofAceticAcid
infoodstuffs,beveragesandothermaterials
Principle:
(acetatekinase)
(1)Aceticacid+ATP→acetyl-phosphate+ADP
(phosphotransacetylase)
(2)Acetyl-phosphate+CoA→acetyl-CoA+Pi
(pyruvatekinase)
(3)ADP+PEP→ATP+pyruvate
(D-lactatedehydrogenase)
(4)Pyruvate+NADH+H+→D-lacticacid+NAD+
Kitsize: * 72assays(manual)/720(microplate)
* Thenumberofmanualtestsperkitcanbedoubledifallvolumesarehalved.
ThiscanbereadilyaccommodatedusingtheMegaQuantTM Wave
Spectrophotometer(D-MQWAVE).
Method: Spectrophotometricat340nm
Reactiontime: ~4min
Detectionlimit: 0.254mg/L
Applicationexamples:
Wine,beer,fruitandfruitjuices,softdrinks,vinegar,vegetables,
pickles,dairyproducts(e.g.cheese),meat,fish,bread,bakeryproducts
(andbakingagents),ketchup,soysauce,mayonnaise,dressings,paper(andcardboard),tea,pharmaceuticals(e.g.infusionsolutions),feedandothermaterials(e.g.biologicalcultures,samples,etc.)
Methodrecognition: Improvedmethod
Advantages
- Improvedassayformat(onlytwoabsorbancereadingsrequired)
- Allreagentsstablefor>2yearsafterpreparation
- PVPincorporatedtopreventtannininhibition
- Veryrapidreaction(~4min)
- Mega-Calc™softwaretoolisavailablefromourwebsiteforhassle-freerawdataprocessing
- Verycompetitiveprice(costpertest)
- Suitableformanualandmicroplateformats
品牌介绍
Megazyme品牌产品简介
来源：作者：人气：2149发表时间：2016-05-19 10:59:00【大 中 小】
Megazyme是一家全球性公司，专注于开发和提供用于饮料、谷物、乳制品、食品、饲料、发酵、生物燃料和葡萄酒产业用的分析试剂、酶和检测试剂盒。Megazyme的许多检测试剂盒产品已经为众多官方科学协会（包括AOAC, AACC , RACI, EBC和ICC等），经过严格的审核，批准认证为官方标准方法，确保以准确、可靠、定量和易于使用的测试方法，满足客户的质量诉求。
Megazyme的主要产品线包括：
◆ 检测试剂盒
◆ 酶
◆ 酶底物
◆ 碳水化合物
◆ 化学品/仪器
官网地址：http://www.megazyme.com
检测试剂盒特色产品：
货号
中文品名
用途
K-ACETAF
乙酸[AF法]检测试剂盒
酶法定量分析乙酸最广泛使用的方法
K-ACHDF
可吸收糖/膳食纤维检测试剂盒
酒精沉淀法测定膳食纤维
K-AMIAR
氨快速检测试剂盒
用于包括葡萄汁、葡萄酒以及其它食品饮料样品中氨含量的快速检测分析。
K-AMYL
直链淀粉/支链淀粉检测试剂盒
谷物淀粉和而粉中直链淀粉/支链淀粉比例和含量检测
K-ARAB
阿拉伯聚糖检测试剂盒
果汁浓缩液中阿拉伯聚糖的检测
K-ASNAM
L-天冬酰胺/L-谷氨酰胺和氨快速检测试剂盒
用于食品工业中丙烯酰胺前体、细胞培养基、以及上清液组分中、L-天冬酰胺，谷氨酰胺和氨的检测分析
K-ASPTM
阿斯巴甜检测试剂盒
专业用于测定饮料和食品中阿斯巴甜含量，操作简单
K-BETA3
β-淀粉酶检测试剂盒
适用于麦芽粉中β-淀粉酶的测定
K-BGLU
混合键β-葡聚糖检测试剂盒
测定谷物、荞麦粉、麦汁、啤酒及其它食品中混合键β-葡聚糖(1,3:1,4-β-D-葡聚糖)的含量
K-CERA
α-淀粉酶检测试剂盒
谷物和发酵液（真菌和细菌）中α-淀粉酶的分析测定
K-CITR
柠檬酸检测试剂盒
快速、可靠地检测食品、饮料和其它物料中柠檬酸（柠檬酸盐）含量
K-DLATE
乳酸快速检测试剂盒
快速、特异性检测饮料、肉类、奶制品和其它食品中L-乳酸和D-乳酸(乳酸盐）含量
K-EBHLG
酵母β-葡聚糖酶检测试剂盒
用于测量和分析酵母中1,3:1,6?-β-葡聚糖，也可以检测1,3-葡聚糖
K-ETSULPH
总亚硫酸检测试剂盒
测定葡萄酒、饮料、食品和其他物料中总亚硫酸含量（按二氧化硫计）的一种简单，高效，可靠的酶法检测方法
K-FRGLMQ
D-果糖/D-葡萄糖[MegaQuant法]检测试剂盒
适用于使用megaquant?色度计（505nm下）测定葡萄、葡萄汁和葡萄酒中D-果糖和D-葡萄糖的含量。
K-FRUC
果聚糖检测试剂盒
含有淀粉、蔗糖和其他糖类的植物提取物和食品中果聚糖的含量测定。
K-FRUGL
D-果糖/D-葡萄糖检测试剂盒
对植物和食品中果糖或葡萄糖含量的酶法紫外分光测定。
K-GALM
半乳甘露聚糖检测试剂盒
食品和植物产品中半乳甘露聚糖的含量检测
K-GLUC
D-葡萄糖[GOPOD]检测试剂盒
谷物提取物中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUHK
D-葡萄糖[HK]检测试剂盒
植物和食品中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUM
葡甘聚糖检测试剂盒
植物和食品中葡甘聚糖的含量测定。
K-INTDF
总膳食纤维检测试剂盒
总膳食纤维特定检测和分析
K-LACGAR
乳糖/D-半乳糖快速检测试剂盒
用于快速检测食品和植物产品中乳糖、D-半乳糖和L-阿拉伯糖
K-LACSU
乳糖/蔗糖/D-葡萄糖检测试剂盒
混合面粉和其它物料中蔗糖、乳糖和D-葡萄糖的测定
K-LACTUL
乳果糖检测试剂盒
特异性、快速和灵敏测量奶基样品中乳果糖含量
K-MANGL
D-甘露糖/D-果糖/D-葡萄糖检测试剂盒
适合测定植物产品和多糖酸性水解产物中D-甘露糖含量
K-MASUG
麦芽糖/蔗糖/D-葡萄糖检测试剂盒
在植物和食品中麦芽糖，蔗糖和葡萄糖的含量检测
K-PECID
胶质识别检测试剂盒
食品配料中果胶的鉴别
K-PHYT
植酸(总磷)检测试剂盒
食品和饲料样品植酸/总磷含量测量的简便方法。不需要通过阴离子交换色谱对植酸纯化，适合于大量样本分析
K-PYRUV
丙酮酸检测试剂盒
在啤酒、葡萄酒、果汁、食品和体液中丙酮酸分析
K-RAFGA
棉子糖/D-半乳糖检测试剂盒
快速测量植物材料和食品中棉子糖和半乳糖含量
K-RAFGL
棉子糖/蔗糖/D-半乳糖检测试剂盒
分析种子和种子粉中D-葡萄糖、蔗糖、棉子糖、水苏糖和毛蕊花糖含量。通过将棉子糖、水苏糖和毛蕊花糖酶解D-葡萄糖、D-果糖和半乳糖，从而测定葡萄糖含量来确定
K-SDAM
淀粉损伤检测试剂盒
谷物面粉中淀粉损伤的检测和分析
K-SUCGL
蔗糖/D-葡萄糖检测试剂盒
饮料、果汁、蜂蜜和食品中蔗糖和葡萄糖的分析
K-SUFRG
蔗糖/D-果糖/D-葡萄糖检测试剂盒
适用于植物和食品中蔗糖、D-葡萄糖和D-果糖的测定
K-TDFR
总膳食纤维检测试剂盒
总膳食纤维检测
K-TREH
海藻糖检测试剂盒
快速、可靠地检测食品、饮料和其它物料中海藻糖含量
K-URAMR
尿素/氨快速检测试剂盒
适用于水、饮料、乳制品和食品中尿素和氨的快速测定
K-URONIC
D-葡萄糖醛酸/D-半乳糖醛酸检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中六元糖醛酸含量（D-葡萄糖醛酸和D-半乳糖醛酸）
K-XYLOSE
D-木糖检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中D-木糖含量
K-YBGL
Beta葡聚糖[酵母和蘑菇]检测试剂盒
检测酵母和蘑菇制品中1,3:1,6-beta-葡聚糖和α-葡聚糖含量
联络我们
