Megazyme/柠檬酸分析试剂盒/K-CITR/72分析（手动）/720分析（微孔板）/840分析（自动分析仪）
商品编号：
K-CITR
品牌：
Megazyme INC
市场价：
¥3080.00
美元价：
1848.00
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
TheCitricAcidtestkitisa flexIBLeandsimplemethodfortherapidandreliablemeasurementandanalysisofcitricacid(citrate)infoods,beveragesandothermaterials.
ExtendedcofactorsstABIlity.Dissolvedcofactorsstablefor>1yearat4oC.
Suitableformanual,auto-analyserandmicroplateformats.
Grapeandwineanalysis:Oenologiststoexploitadvancedtestkits.
Charnock,S.C.&McCleary,B.V.(2005).RevuedesEnology,117,1-5.
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Itiswithoutdoubtthattestingplaysapivotalrolethroughoutthewholeofthevinificationprocess.Toproducethebestpossiblequalitywineandtominimiseprocessproblemssuchas“stuck”fermentationortroublesomeinfections,itisnowrecognisedthatifpossibletestingshouldbeginpriortoharvestingofthegrapesandcontinuethroughtobottling.TrADItionalmethodsofwineanalysisareoftenexpensive,timeconsuming,requireeitherelaborateequipmentorspecialistexpertiseandfrequentlylackaccuracy.However,enzymaticbio-analysisenablestheaccuratemeasurementofthevastmajorityofanalytesofinteresttothewinemaker,usingjustonepieceofapparatus,thespectrophotometer(seepreviousissueNo.116foradetailedtechnicalreview).Grapejuiceandwineareamenabletoenzymatictestingasbeingliquidstheyarehomogenous,easytomanipulate,andcangenerallybeanalysedwithoutanysamplepreparation.
Megazyme“advanced”winetestkitsgeneralcharacteristicsandvalidation.
Charnock,S.J.,McCleary,B.V.,Daverede,C.&Gallant,P.(2006).ReveuedesOenologues,120,1-5.
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ManyoftheenzymatictestkitsareofficialmethodsofprestigiousorganisationssuchastheAssociationofOfficialAnalyticalChemicals(AOAC)andtheAmericanAssociationofCerealChemists(AACC)inresponsetotheinterestfromoenologists.Megazymedecidedtouseitslonghistoryofenzymaticbio-analysistomakeasignificantcontributiontothewineindustry,bythedevelopmentofarangeofadvancedenzymatictestkits.Thistaskhasnowbeensuccessfullycompletedthroughthestrategicandcomprehensiveprocessofidentifyinglimitationsofexistingenzymaticbio-analysistestkitswheretheyoccurred,andthenusingadvancedtechniques,suchasmolecularBIOLOGy(photo1),torapidlyovercomethem.Noveltestkitshavealsobeendevelopedforanalytesofemerginginteresttotheoenologist,suchasyeastavailablenitrogen(YAN;seepages2-3ofissue117article),orwherepreviouslyenzymesweresimplyeithernotavailable,orweretooexpensivetoemploy,suchasforD-mannitolanalysis.
Influenceofstarterculturesontheantioxidantactivityofkombuchabeverage.
Malbaša,R.V.,Lončar,E.S.,Vitas,J.S.&Čanadanović-Brunet,J.M.(2011).FoodChemistry,127(4),1727-1731.
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Thispaperinvestigatestheinfluenceofstartercultures,obtainedfromkombuchaisolates,ontheantioxidantactivityofkombuchabeverages.Threestartercultureswereusedasfollows:(1)mixedcultureofaceticbacteriaandZygosaccharomycessp.(SC1);(2)mixedcultureofaceticbacteriaandSaccharomycescerevisiae(SC2);aswellas(3)nativelocalkombucha.Thestartercultureswereaddedtoblackandgreenteasweetenedwith7%ofsucrose.Fermentationwascarriedoutat28°Cfor10days.AntioxidantactivitytohydroxylandDPPHradicalswasmonitored.KombuchabeverageonblackteahasshownthehighestantioxidantactivitytobothtypesofradicalswithstarterSC1,whilethegreenteabeveragehasshownthehighestactivitywithnativekombucha.Themainreasonforthedifferentantioxidantactivities,besideteacomposition,wasascribedtodifferingproductionofbothvitaminCandtotalorganicacidsintheinvestigatedsystems.
Constructionandcharacterizationofthreelactatedehydrogenase-negativeEnterococcusfaecalisV583mutants.
Jönsson,M.,Saleihan,Z.,Nes,I.F.&Holo,H.(2009).AppliedandEnvironmentalMicrobiology,75(14),4901-4903.
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TherolesofthetwoldhgenesofEnterococcusfaecaliswerestudiedusingknockoutmutants.Deletionofldh-1causesametabolicshiftfromhomolacticfermentationtoethanol,formate,andacetoinproduction,withahighlevelofformateproductionevenunderaerobicconditions.Ldh-2playsonlyaminorroleinlactateproduction.
Isolationoflipaseandcitricacidproducingyeastsfromagro-industrialwastewater.
Mafakher,L.,Mirbagheri,M.,Darvishi,F.,Nahvi,I.,Zarkesh-Esfahani,H.&Emtiazi,G.(2010).NewBiotechnology,27(4),337-340.
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Productionofagro-industrialwastepollutantshasbecomeamajorproblemformanyindustries.However,agro-industrialwastesalsocanprovidealternativesubstratesforindustryandtheirutilizationinthismannermayhelpsolvepollutionproblems.Theaimofthisstudywastoisolateyeastsfromwastewatertreatmentplantsthatcouldbeusedtoremovepollutantssuchasglycerol,paraffinandcrudeoilfromtheagro-industrialwastewater.Inthisstudyatotalof300yeastisolateswereobtainedfromsamplesofagro-industrialwastes,andtwostrains(M1andM2)wereinvestigatedfortheirabilitytoproducevaluableproductssuchaslipaseandcitricacid.IdentificationtestsshowedthattheseisolatesbelongedtothespeciesYarrowialipolytica.TheY.lipolyticaM1andM2strainsproducedmaximumlevelsoflipase(11and8.3U/ml,respectively)onoliveoil,andhighlevelsofcitricacid(27and8g/l,respectively)oncitricacidfermentationmedium.
ComparisonofeffectsofdietarycoconutoilandanimalfatblendonlactationalperformanceofHolsteincowsfedahigh-starchdiet.
Hollmann,M.&Beede,D.K.(2012).JournalofDairyScience,95(3),1484-1499.
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Dietarymedium-chainfattyacids(C8:0throughC12:0)areresearchedfortheirpotentialtoreduceentericmethaneemissionsandtoincreaseNutilizationefficiencyinruminants.Weaimedto1)comparecoconutoil(CNO;∼60%medium-chainfattyacids)withasourceoflong-chainfattyacids(animalfatblend;AFB)onlactationalresponsesinahigh-starchdietand2)determinetheeffectofdifferentdietaryconcentrationsofCNOondrymatterintake(DMI).Inexperiment1,thecontroldiet(CTRL)contained(drybasis)40%forage(71%cornsilage,andalfalfahayandhaylage),26%NDF,and35%starch.Isonitrogenoustreatmentdietscontained5.0%ofAFB(5%-AFB),CNO(5%-CNO),ora1-to-1mixtureofAFBandCNO(5%-AFB-CNO)and0.8%cornglutenmealinplaceofcorngrain.Thirty-twomultiparousdairycows(201±46dpostpartum;42.0±5.5kg/d3.5%fat-correctedmilkyield)wereadaptedtoCTRL,blockedbymilkyield,andrandomlyassignedto1of4treatmentdietsfor21dwithsamplesanddatacollectedfromd15through21.Treatment5%-CNOdecreasedDMImarkedlyandprecipitouslyandwasdiscontinuedafterd5.Inwk3,5%-AFBandespecially5%-AFB-CNOloweredtotal-tractNDFdigestedvs.CTRL(2.6vs.1.8vs.3.1kg/d,respectively),likelybecausefattreatmentsreducedDMIand5%-AFB-CNOimpairedtotal-tractNDFdigestibility.Milkfatconcentrationswere3.10%(CTRL),2.51%(5%-AFB),and1.97%(5%-AFB-CNO)andcorrelatednegativelytoconcentrationsofC18:2trans-10,cis-12inmilkfat.Additionally,5%-AFBand5%-AFB-CNOtendedtolowermilkyieldanddecreasedyieldsofsolids-correctedmilkandmilkproteincomparedwithCTRL.Fattreatmentsdecreasedmilklactoseconcentration,butincreasedmilkcitrateconcentration.Moreover,cowsfed5%-AFB-CNOproducedlesssolids-correctedmilkthandidcowsfed5%-AFB.Inexperiment2,dietssimilartoCTRLcontained2.0,3.0,or4.0%CNO.Fifteenmultiparouscows(219±42dpostpartum;42.1±7.0kgmilkyield;mean±SD)wereblockedbyDMIandrandomlyassignedto1of3treatmentdietsforan8-devaluation.DietaryconcentrationofCNOaffectedDMI,withthegreatestdepressionat4.0%CNO.Overall,dietaryCNOdepressedDMIandNDFdigestibilityofahigh-starchdietcomparedwithAFB.FeedingCNOtolactatingcowsequaltoorgreaterthan2.5%decreasedlactationalperformanceorDMI.
EnhancedproductionofcitricacidinYarrowialipolyticabyTritonX-100.
Mirbagheri,M.,Nahvi,I.,Emtiazi,G.&Darvishi,F.(2011).AppliedBiochemistryandBiotechnology,165(3),1068-1074.
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Variouschemicalsurfactantscouldaffectpermeabilityofyeastcells.Inthisstudy,effectsofthesurfactantadditionuponyeastcellspermeabilityandcitricacid(CA)productionbyYarrowialipolyticastrainsDSM3286andM7wereinvestigated.TheadditionofTritonX-100increased1.4–1.8-foldofthemaximumCAquantityachievedforbothstrains,withfinalCAconcentrationsrangingbetween75–85g/lthatcorrespondtoCAconversionyieldsperunitofglucoseconsumedof~0.80–0.84g/g.ScanningelectronmicrographsofyeastcellsshowedthatthecellstreatedwithTritonX-100hadalteredcellstructureandweresmallerandnarrowercomparedwiththenon-treatedones.TheresultsshowedthatTritonX-100couldbeusedinordertoincreasetheefficiencyofCAproductionbyY.lipolyticastrains.
NaturalvariationforFe-efficiencyisassociatedwithupregulationofStrategyImechanismsandenhancedcitrateandethylenesynthesisinPisumsativumL.
Kabir,A.H.,Paltridge,N.G.,Able,A.J.,Paull,J.G.&Stangoulis,J.C.R.(2012).Planta,235(6),1409-1419.
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Iron(Fe)-deficiencyisacommonabioticstressinPisumsativumL.growninmanypartsoftheworld.TheaimofthestudywastoinvestigatevariationintolerancetoFedeficiencyintwopeagenotypes,Santi(Fe-efficient)andParafield(Fe-inefficient).Fedeficiencycausedgreaterdeclinesinchlorophyllscore,leafFeconcentrationandroot–shootdevelopmentinParafieldcomparedtoSanti,suggestinggreaterFe-efficiencyinSanti.FechelatereductaseactivityandethyleneproductionwereincreasedintherootsofSantiandtoalesserextentinParafieldunderFedeficiency,whileprotonextrusionwasonlyoccurredinSanti.Moreover,expressionoftheFechelatereductasegene,FRO1,andFetransporter,RIT1wereupregulatedinFe-deficientrootsofSanti.ExpressionofHA1(protonextrusion)wasalsosignificantlyhigherinSantiwhencomparedtoParafieldgrowninFe-deficientconditions.FurThermore,theapplicationoftheethylenebiosynthesisinhibitor,1-aminoisobutyricacidreducedtheFechelatereductaseactivity,supportingadirectroleforethyleneinitsinduction.AsignificantincreaseinrootcitratewasonlyobservedinSantiunderFedeficiencyindicatingaroleforcitrateintheFe-efficiencymechanism.Takentogether,ourphysiologicalandmoleculardataindicatethatgenotypicvariationintolerancetoFedeficiencyinSantiandParafieldplantsisaresultofvariationinanumberofStrategyImechanismsandalsosuggestadirectroleforethyleneinFereductaseactivity.Thepeacultivar,SantiprovidesanewsourceofFe-efficiencythatcanbeexploitedtobreedmoreFe-efficientpeas.
Apaf-1-deficientfogmousecellapoptosisinvolveshypo-polarizationofthemitochondrialinnermembrane,ATPdepletionandcitrateaccumulation.
Katoh,I.,Sato,S.,Fukunishi,N.,Yoshida,H.,Imai,T.&Kurata,S.I.(2008).Cellresearch,18(12),1210-1219.
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Toexplorehowtheintrinsicapoptosispathwayiscontrolledinthespontaneousfog(forebrainovergrowth)mutantmicewithanApaf1splicingdeficiency,weexaminedspleenandbonemarrowcellsfromApaf1+/+(+/+)andApaf1fog/fog(fog/fog)miceforinitiatorcaspase-9activationbycellularstresses.Whenthemitochondrialinnermembranepotential(Δψm)wasdisruptedbystaurosporine,+/+cellsbutnotfog/fogcellsactivatedcaspase-9tocauseapoptosis,indicatingthelackofapoptosome(apoptosisproteaseactivatingfactor1(Apaf-1)/cytochromec/(d)ATP/procaspase-9)functioninfog/fogcells.However,whenamarginal(~20%)decreaseinΔψmwascausedbyhydrogenperoxide(0.1mM),peroxynitritedonor3-morpholinosydnonimine(0.1mM)andUV-Cirradiation(20J/m2),both+/+andfog/fogcellstriggeredprocaspase-9auto-processinganditsdownstreamcascadeactivation.Supportingourpreviousresults,procaspase-9pre-existinginthemitochondriainduceditsauto-processingbeforethecytosoliccaspaseactivationregardlessofthegenotypes.CellularATPconcentrationsignificantlydecreasedunderthehypoactiveΔψmcondition.Furthermore,wedetectedaccumulationofcitrate,akosmotropeknowntofacilitateprocaspase-9dimerization,probablyduetoafeedbackcontroloftheKrebscyclebytheelectrontransfersystem.Thus,mitochondrialinsitucaspase-9activationmaybecausedbythemajormetabolicreactionsinresponsetophysiologicalstresses,whichmayrepresentamodeofApaf-1-independentapoptosishypothesizedfromrecentgeneticstudies.
Relationshipsbetweenacceptanceofsourtasteandfruitintakesin18-month-oldinfants.
Blossfeld,I.,Collins,A.,Boland,S.,Baixauli,R.,Kiely,M.&Delahunty,C.(2007).BritishJournalofNutrition,98(05),1084-1091.
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Thepresentstudyexaminedwhetherinfantsshowanacceptanceforextremesourtastesandwhetheracceptanceofsourtasteisrelatedtoinfants"fruitintake.Fruitintakeoffifty-threeinfantsat6,12and18monthswasassessedusing3dfoodrecords.Souracceptanceoftheseinfantswasstudiedat18•1(sd1•5)months.Acceptanceforfoursolutionsdifferingincitricacidconcentrations(0•00m,0•013m,0•029mand0•065m)wasmeasuredbyallowinginfantsadlibitumingestionofeachsolutionoverbrieftimeperiods.Thebasesolutiontowhichcitricacidwasaddedwasblackcurrantsquashdilutedinwater.Infants"relativeintakeofeachsolutionwasusedasameasureofsouracceptance.At18months,twelveinfantsreadilyacceptedthetwohighestcitricacidconcentrations,whereastheremaininginfantsrejectedthese.Infantswhoacceptedthemostsoursolutionshadasignificantlyhigherfruitintake(P=0•025)andahigherfruitvariety(P=0•015)at18monthsthantheinfantswhorejectedthehighlysourtaste.Furthermore,infantswhoacceptedthemostsoursolutionsconsumedfruitsmorefrequentlyat18months(Χ25•1;P=0•024).Infantswhoacceptedthesourestsolutionsalsohadahigherfruitintakeat6months,andasignificantlyhigherincreaseintheirfruitintakefrom12to18months.Thisisthefirstscientificstudythatdemonstratestheacceptanceofsourtastesinsomeinfantsattheageof18months.Furthermore,thepresentresultssuggestarelationshipbetweenacceptanceofsourtastesandinfants"fruitintakes.
TaxonomiccharacterizationandpotentialbiotechnologicalapplicationsofYarrowialipolyticaisolatedfrommeatandmeatproducts.
Mirbagheri,M.,Nahvi,I.,Emtiazi,G.,Mafakher,L.&Darvishi,F.(2012).JundishapurJournalofMicrobiology,5(1),346-351.
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Background:SomespeciesofyeastsuchasYarrowialipolyticaproducecitricacid,lipases,single-celloil,etc.Y.lipolyticacandegraderenewable,low-costsubstratestoproduceorganicacidslikecitricacid,moreefficientlythanAspergillusniger,andresultinhigherproductyieldandlesserwasteproductionandtoxicity.Objectives:Theaimofthisstudywastoisolateyeaststrainswithpotentialforuseinbiotechnologicalapplicationssuchasproductionofcitricacidandlipase.MaterialsandMethods:Foryeaststrainscreening,weisolated179yeaststrainsfrommeatandmeatproductsthatwerepreparedattheRAKandPegahfactoriesinIsfahan,Iran.Differentmediawereusedforscreeningofyeastcoloniesandforanalysesofcitricacidandlipaseproduction;theproductionofthesemetaboliteswasassayedovertime.Results:Oneoftheyeaststrainsisolatedfrompoultryproduced55.5g/Lofcitricacidand12.3U/mLoflipase.BiochemicalandmoleculartestsshowedthatthisstrainbelongedtothespeciesY.lipolytica.MolecularidentificationwasconfirmedbyDNAsequencing,andthestrainwasnamedY.lipolyticaM7(GenBankaccessionnumber,HM011048).Conclusions:Theresultsofthisstudysuggestthatmeatanditsproducts,especiallypoultryproducts,aresuitablesourcesforisolationofyeaststrainsthatproducetwobiotechnologicallyvaluableproducts-citricacidandlipase.TheyeaststrainY.lipolyticaM7canbeusedforcitricacidproductioninbioreactor.
Geneticmappingofa7RAltoleranceQTLintriticale(xTriticosecaleWittmack).
Niedziela,A.,Bednarek,P.T.,Labudda,M.,Mańkowski,D.R.&Anioł,A.(2014).JournalofAppliedGenetics,55(1),1-14.
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Triticale(xTriticosecaleWittmack)isarelativelynewcerealcrop.InPoland,triticaleisgrownon12%ofarableland(http://www.stat.gov.pl).Thereisanincreasinginterestinitscultivationduetoloweredproductioncostsandincreasedadaptationtoadverseenvironmentalconditions.However,ithasaninsufficienttolerancetothepresenceofaluminumions(Al3+)inthesoil.Thenumberofgenescontrollingaluminumtoleranceintriticaleandtheirchromosomallocationisnotknown.TwoF2mappingbiparentalpopulations(MP1andMP15)segregatingforaluminum(Al)toleranceweretestedwithAFLP,SSR,DArT,andspecificPCRMarkers.Geneticmappingenabledtheconstructionoflinkagegroupsrepresentingchromosomes7R,5Rand2B.Obtainedlinkagegroupswerecommonforbothmappingpopulationsandmostlyincludedthesamemarkers.Compositeintervalmapping(CIM)allowedidentificationofasingleQTLthatmappedtothe7Rchromosomeandexplained25%(MP1)and36%(MP15)ofphenotypicvariation.TheB1,B26andXscm150markerswere0.04cMand0.02cMfromthemaximumoftheLODfunctionintheMP1andMP15,respectivelyandwerehighlyassociatedwithaluminumtoleranceasindicatedbyKruskal–Wallisnonparametrictest.Moreover,themolecularmarkersB1,B26,Xrems1162andXscm92,previouslyassociatedwiththeAlt4locusthatencodedanaluminum-activatedmalatetransporter(ScALMT1)thatwasinvolvedinAltoleranceinrye(Secalecereale)alsomappedwithinQTL.BiochemicalanalysisofplantsrepresentedMP1andMP15mappingpopulationsconfirmedthattheQTLlocatedon7RchromosomeinbothmappingpopulationsisresponsibleforAltolerance.
CompromisedLactobacillushelveticusstarteractivityinthepresenceoffacultativeheterofermentativeLactobacilluscaseiDPC6987resultsinatypicaleyeformationinSwiss-typecheese.
O’Sullivan,D.J.,McSweeney,P.L.H.,Cotter,P.D.,Giblin,L.&Sheehan,J.J.(2016).Journalofdairyscience,99(4),2625-2640.
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Nonstarterlacticacidbacteriaarecommonlyimplicatedinundesirablegasformationinseveralvarieties,includingCheddar,Dutch-,andSwiss-typecheeses,primarilyduetotheirabilitytofermentawidevarietyofsubstrates.Thiseffectcanbemagnifiedduetofactorsthatdetrimentallyaffectthecompositionoractivityofstarterbacteria,resultinginthepresenceofgreaterthannormalamountsoffermentablecarbohydratesandcitrate.TheobjectiveofthisstudywastodeterminethepotentialforafacultativelyheterofermentativeLactobacillus(LactobacilluscaseiDPC6987)isolatedfromacheeseplantenvironmenttopromotegasdefectsintheeventofcompromisedstarteractivity.ASwiss-typecheesewasmanufactured,atpilotscaleandintriplicate,containingatypicalstarterculture(StreptococcusthermophilusandLactobacillushelveticus)togetherwithpropionicacidbacteria.Lactobacillushelveticuspopulationswereomittedincertainvatstomimicstarterfailure.LactobacilluscaseiDPC6987wasaddedtoeachexperimentalvatat4logcfu/g.CheesecompositionalanalysisandX-raycomputedtomographyrevealedthatthefailureofstarterbacteria,inthiscaseL.helveticus,coupledwiththepresenceofafaculativelyheterofermentativeLactobacillus(L.casei)ledtoexcessiveeyeformationduringripening.Theavailabilityofexcessamountsoflactose,galactose,andcitrateduringtheinitialripeningstageslikelyprovidedtheheterofermentativeL.caseiwithsufficientsubstratesforgasformation.TheaccrualofthesefermentablesubstrateswasnotableincheeseslackingtheL.helveticusstarterpopulation.Theresultsofthisstudyarecommerciallyrelevant,astheydemonstratetheimportanceofviabilityofstarterpopulationsandthecontrolofspecificnonstarterlacticacidbacteriatoensureappropriateeyeformationinSwiss-typecheese.
Chemicalcompositionandinvitroantimicrobialandcytotoxicactivitiesofplum(PrunusdomesticaL.)wine.
Miljić,U.,Puškaš,V.,Velićanski,A.,Mašković,P.,Cvetković,D.&Vujić,J.(2016).JournaloftheInstituteofBrewing,122(2),342-349.
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Amoderateintakeofwineisassociatedwithapositiveimpactonhumanhealthowingtotheeffectsofimportantbiologicallyactivecomponentspresentinthewineinlargeamounts.Theaimofthisstudywastoexaminethechemicalcompositionandtoassessantimicrobialandcytotoxicactivitiesoffruitwinesproducedfromthreeplumvarieties(Čačanskarana,ČačanskalepoticaandPožegača)commonlygrowninSerbiaasanapproachtoassessthequalityandacceptabilityofthesewinesasafunctionalfood.Furthermore,theactivityofaseriesofcontrolsampleswasassessedinordertodeterminecomponentsfromthewinethatareresponsibleforitsfunctionalproperties.Theplumwinesproducedshowedconsiderableantimicrobialactivityagainstsixbacterialandtwoyeaststrainsusedinthisstudy.Inadditiontoantimicrobialactivity,theplumwinesshowedasignificantcytotoxiceffect(IC50 < 50 µg mL-1)onthegrowthofthreetestedcancercelllines(Hep2c,RDandL2OB).Regardingthedeterminedactivities,Čačanskaranaplumwineachievedthebestresults.Theresultsindicatedthattheantimicrobialactivityoftheplumwineswas,inlargepart,basedontheeffectsofthetotalacidsandthepHvalue,whilethecontributionofethanolandthecontentofthephenoliccompoundswerenotsignificant.Similarconclusionsweredrawnregardingthecytotoxicactivityofthisfruitwine.Theresultscanbeseenasacontributiontotheglobalacceptanceoffruitwinesasafunctionalfood,withtheaccentplacedonmoderateconsumption.Animportantadvantageoffruitwines(inparticularplumwine),comparedwithtraditionalgrapewine,istheirloweralcoholcontent.
UV-methodforthedeterminationofCitricAcidinfoods,
beveragesandothermaterials
Principle:
(citratelyase)
(1)Citrate→oxaloacetate+acetate
(L-malatedehydrogenase)
(2)Oxaloacetate+NADH+H+→L-malate+NAD+
(D-lactatedehydrogenase)
(3)Pyruvate+NADH+H+→D-lactate+NAD+
Kitsize: *72assays(manual)/720(microplate)
/840(auto-analyser)* Thenumberofmanualtestsperkitcanbedoubledifallvolumesarehalved.
ThiscanbereadilyaccommodatedusingtheMegaQuantTM Wave
Spectrophotometer(D-MQWAVE).Method: Spectrophotometricat340nm
Reactiontime: ~5min
Detectionlimit: 0.491mg/L
Applicationexamples:
Grapejuice,wine,beer,fruitjuices,softdrinks,tea,dairyproducts
(e.g.cheese),meat,processedmeat,vegetableandfruitproducts,
bakeryproducts,paper,pharmaceuticals,cosmeticsandother
materials(e.g.biologicalcultures,samples,etc.)
Methodrecognition:
MethodsbasedonthisprinciplehavebeenacceptedbyMEBAK,OIV,
EU,ISO2963,AOACandIFU22
(Note:Iftheenzymeoxaloacetatedecarboxylaseispresentinthesample,some
oftheoxaloacetateproductisconvertedtopyruvate.Therefore,toensurecitric
acidismeasuredquantitatively,D-lactatedehydrogenase(D-LDH)isemployed
toefficientlyconvertanypyruvateproducedintoD-lactateandNAD+).
Advantages
- Reconstitutedcitratelyasestablefor4weeksat4°C/6monthsat-20°C
- Buffer/cofactor/enzymetabletsforefficientuseofkitcomponents
- PVPincorporatedtopreventtannininhibition
- Verycompetitiveprice(costpertest)
- Mega-Calc™softwaretoolisavailablefromourwebsiteforhassle-freerawdataprocessing
- Standardincluded
- Extendedcofactorsstability
- Suitableformanual,microplateandauto-analyserformats
品牌介绍
Megazyme品牌产品简介
来源：作者：人气：2149发表时间：2016-05-19 10:59:00【大 中 小】
Megazyme是一家全球性公司，专注于开发和提供用于饮料、谷物、乳制品、食品、饲料、发酵、生物燃料和葡萄酒产业用的分析试剂、酶和检测试剂盒。Megazyme的许多检测试剂盒产品已经为众多官方科学协会（包括AOAC, AACC , RACI, EBC和ICC等），经过严格的审核，批准认证为官方标准方法，确保以准确、可靠、定量和易于使用的测试方法，满足客户的质量诉求。
Megazyme的主要产品线包括：
◆ 检测试剂盒
◆ 酶
◆ 酶底物
◆ 碳水化合物
◆ 化学品/仪器
官网地址：http://www.megazyme.com
检测试剂盒特色产品：
货号
中文品名
用途
K-ACETAF
乙酸[AF法]检测试剂盒
酶法定量分析乙酸最广泛使用的方法
K-ACHDF
可吸收糖/膳食纤维检测试剂盒
酒精沉淀法测定膳食纤维
K-AMIAR
氨快速检测试剂盒
用于包括葡萄汁、葡萄酒以及其它食品饮料样品中氨含量的快速检测分析。
K-AMYL
直链淀粉/支链淀粉检测试剂盒
谷物淀粉和而粉中直链淀粉/支链淀粉比例和含量检测
K-ARAB
阿拉伯聚糖检测试剂盒
果汁浓缩液中阿拉伯聚糖的检测
K-ASNAM
L-天冬酰胺/L-谷氨酰胺和氨快速检测试剂盒
用于食品工业中丙烯酰胺前体、细胞培养基、以及上清液组分中、L-天冬酰胺，谷氨酰胺和氨的检测分析
K-ASPTM
阿斯巴甜检测试剂盒
专业用于测定饮料和食品中阿斯巴甜含量，操作简单
K-BETA3
β-淀粉酶检测试剂盒
适用于麦芽粉中β-淀粉酶的测定
K-BGLU
混合键β-葡聚糖检测试剂盒
测定谷物、荞麦粉、麦汁、啤酒及其它食品中混合键β-葡聚糖(1,3:1,4-β-D-葡聚糖)的含量
K-CERA
α-淀粉酶检测试剂盒
谷物和发酵液（真菌和细菌）中α-淀粉酶的分析测定
K-CITR
柠檬酸检测试剂盒
快速、可靠地检测食品、饮料和其它物料中柠檬酸（柠檬酸盐）含量
K-DLATE
乳酸快速检测试剂盒
快速、特异性检测饮料、肉类、奶制品和其它食品中L-乳酸和D-乳酸(乳酸盐）含量
K-EBHLG
酵母β-葡聚糖酶检测试剂盒
用于测量和分析酵母中1,3:1,6?-β-葡聚糖，也可以检测1,3-葡聚糖
K-ETSULPH
总亚硫酸检测试剂盒
测定葡萄酒、饮料、食品和其他物料中总亚硫酸含量（按二氧化硫计）的一种简单，高效，可靠的酶法检测方法
K-FRGLMQ
D-果糖/D-葡萄糖[MegaQuant法]检测试剂盒
适用于使用megaquant?色度计（505nm下）测定葡萄、葡萄汁和葡萄酒中D-果糖和D-葡萄糖的含量。
K-FRUC
果聚糖检测试剂盒
含有淀粉、蔗糖和其他糖类的植物提取物和食品中果聚糖的含量测定。
K-FRUGL
D-果糖/D-葡萄糖检测试剂盒
对植物和食品中果糖或葡萄糖含量的酶法紫外分光测定。
K-GALM
半乳甘露聚糖检测试剂盒
食品和植物产品中半乳甘露聚糖的含量检测
K-GLUC
D-葡萄糖[GOPOD]检测试剂盒
谷物提取物中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUHK
D-葡萄糖[HK]检测试剂盒
植物和食品中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUM
葡甘聚糖检测试剂盒
植物和食品中葡甘聚糖的含量测定。
K-INTDF
总膳食纤维检测试剂盒
总膳食纤维特定检测和分析
K-LACGAR
乳糖/D-半乳糖快速检测试剂盒
用于快速检测食品和植物产品中乳糖、D-半乳糖和L-阿拉伯糖
K-LACSU
乳糖/蔗糖/D-葡萄糖检测试剂盒
混合面粉和其它物料中蔗糖、乳糖和D-葡萄糖的测定
K-LACTUL
乳果糖检测试剂盒
特异性、快速和灵敏测量奶基样品中乳果糖含量
K-MANGL
D-甘露糖/D-果糖/D-葡萄糖检测试剂盒
适合测定植物产品和多糖酸性水解产物中D-甘露糖含量
K-MASUG
麦芽糖/蔗糖/D-葡萄糖检测试剂盒
在植物和食品中麦芽糖，蔗糖和葡萄糖的含量检测
K-PECID
胶质识别检测试剂盒
食品配料中果胶的鉴别
K-PHYT
植酸(总磷)检测试剂盒
食品和饲料样品植酸/总磷含量测量的简便方法。不需要通过阴离子交换色谱对植酸纯化，适合于大量样本分析
K-PYRUV
丙酮酸检测试剂盒
在啤酒、葡萄酒、果汁、食品和体液中丙酮酸分析
K-RAFGA
棉子糖/D-半乳糖检测试剂盒
快速测量植物材料和食品中棉子糖和半乳糖含量
K-RAFGL
棉子糖/蔗糖/D-半乳糖检测试剂盒
分析种子和种子粉中D-葡萄糖、蔗糖、棉子糖、水苏糖和毛蕊花糖含量。通过将棉子糖、水苏糖和毛蕊花糖酶解D-葡萄糖、D-果糖和半乳糖，从而测定葡萄糖含量来确定
K-SDAM
淀粉损伤检测试剂盒
谷物面粉中淀粉损伤的检测和分析
K-SUCGL
蔗糖/D-葡萄糖检测试剂盒
饮料、果汁、蜂蜜和食品中蔗糖和葡萄糖的分析
K-SUFRG
蔗糖/D-果糖/D-葡萄糖检测试剂盒
适用于植物和食品中蔗糖、D-葡萄糖和D-果糖的测定
K-TDFR
总膳食纤维检测试剂盒
总膳食纤维检测
K-TREH
海藻糖检测试剂盒
快速、可靠地检测食品、饮料和其它物料中海藻糖含量
K-URAMR
尿素/氨快速检测试剂盒
适用于水、饮料、乳制品和食品中尿素和氨的快速测定
K-URONIC
D-葡萄糖醛酸/D-半乳糖醛酸检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中六元糖醛酸含量（D-葡萄糖醛酸和D-半乳糖醛酸）
K-XYLOSE
D-木糖检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中D-木糖含量
K-YBGL
Beta葡聚糖[酵母和蘑菇]检测试剂盒
检测酵母和蘑菇制品中1,3:1,6-beta-葡聚糖和α-葡聚糖含量
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