Megazyme/L-谷氨酸分析试剂盒/K-GLUT/60分析（手动）/600分析（微孔板）/700分析（自动分析仪）
商品编号：
K-GLUT
品牌：
Megazyme INC
市场价：
¥2260.00
美元价：
1356.00
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
TheL-GlutamicAcidtestkit isasimple,reliable,rapidandaccuratemethodforthemeasurementandanalysisofL-glutamate(MSG)infoodstuffs.
Suitableformanual,auto-analyserandmicroplateformats.
SurfactanttodyebindingdegreebasedapproachfortheselectivedeterminationofL-glutamateinfoodstuffs.
Pedraza,A.,Sicilia,M.D.,Rubio,S.&Pérez-Bendito,D.(2007).AnalyticalandBioanalyticalChemistry,389(7-8),2297-2302.
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AselectivemethodforthedeterminationofL-glutamateinfoodstuffshasbeendeveloped.ItwasbasedonthecompetitionestablishedbetweentheanalyteandthedyeCoomassiebrilliantblueG(CBBG)tointeractwiththesurfactantdidodecyldimethylammoniumbromide(DDABr).ThemeasurementparameterwastheamountofDDABrrequiredtoreachagivendye-to-surfactantbindingdegree.ItwasobtainedbyphotometrictitrationonthebasisofthechangesobservedinthespectralcharacteristicsofthedyewhenCBBG–DDABraggregateswereformed.ThecalibrationgraphobtainedwaslinearintheL-glutamateconcentrationinterval0.2–5mM(detectionlimit0.05mM).Thehighselectivityoftheproposedmethod(otheraminoacidsandfoodadditivesdidnotinterfereattheconcentrationspresentinfoodstuffs)permittedthedirectanalysisoffoodsamplesafterdissolutionoftheanalyteinhotwater.TheaccuracyofthesurfactanttothedyebindingdegreemethodwasdemonstratedbydeterminingL-glutamateindifferentkindsoffoodstuffs(liquidanddriedsoups,seasonings,pastasaucesanddriedmushroomcreams)andcomparingtheresultsobtainedwiththoseprovidedbythecommercialBoehringerMannheimessay.
ThericeR2R3-MYBtranscriptionfactorOsMYB55isinvolvedinthetolerancetohightemperatureandmodulatesaminoacidmetabolism.
El-kereamy,A.,Bi,Y.M.,Ranathunge,K.,Beatty,P.H.,Good,A.G.&Rothstein,S.J.(2012).PloSone,7(12),e52030.
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Temperatureshigherthantheoptimumnegativelyaffectsplantgrowthanddevelopment.Tolerancetohightemperatureisacomplexprocessthatinvolvesseveralpathways.Understandingthisprocess,especiallyincropssuchasrice,isessentialtoprepareforpredictedclimatechangesduetoglobalwarming.Here,weshowthatOsMYB55isinducedbyhightemperatureandoverexpressionofOsMYB55resultedinimprovedplantgrowthunderhightemperatureanddecreasedthenegativeeffectofhightemperatureongrainyield.Transcriptomeanalysisrevealedanincreaseinexpressionofseveralgenesinvolvedinaminoacidsmetabolism.WedemonstratethatOsMYB55bindstothepromoterregionsoftargetgenesanddirectlyactivatesexpressionofsomeofthosegenesincludingglutaminesynthetase(OsGS1;2)glutamineamidotransferase(GAT1)andglutamatedecarboxylase3(GAD3).OsMYB55overexpressionresultedinanincreaseintotalaminoacidcontentandoftheindividualaminoacidsproducedbytheactivationoftheabovementionedgenesandknownfortheirrolesinstresstolerance,namelyL-glutamicacid,GABAandarginineespeciallyunderhightemperaturecondition.Inconclusion,overexpressionofOsMYB55improvesriceplanttolerancetohightemperature,andthishightoleranceisassociatedwithenhancedaminoacidmetabolismthroughtranscriptionactivation.
Astrocyticglutamatetransporter-dependentneuroprotectionagainstglutamatetoxicity:Aninvitrostudyofmaslinicacid.
Qian,Y.,Guan,T.,Tang,X.,Huang,L.,Huang,M.,Li,Y.,Sun,H.,Yu,R.&Zhang,F.(2011).EuropeanJournalofPharmacology,651(1-3),59-65.
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TheastrocyticglutamatetransportersGLAST/EAAT1andGLT-1/EAAT2arecrucialfortheremovalofglutamatefromthesynapticcleftandareessentialformaintainingalowconcentrationofextracellularglutamateinthebrain.Enhancedtransporterexpressionisneuroprotective.Inthepresentstudy,wetestedtheneuropotectiveeffectsofmaslinicacid,anaturalproductfromtheOleaeuropaeaplant,onculturesofprimaryneuronsfromthecerebralcortex.Studiesshowedthatastrocyte-conditionedmediumfrommaslinicacid-treatedastrocytesdose-dependentlypromotedneuronsurvivalduringglutamatetoxicitybyenhancingextracellularglutamateclearance.Real-timePCRandwesternblotanalysisrevealedthatmaslinicacidpre-treatmentsignificantlyincreasedtheexpressionofGLASTandGLT-1attheproteinandmRNAlevels.Inaddition,thisneuroprotectionwasabolishedbytheglutamatetransporterinhibitor,L-Threohydroxyaspartate(THA),inaco-cultureofastrocytesandneurons.Thesefindingssuggestthatmaslinicacidregulatestheextracellularglutamateconcentrationbyincreasingtheexpressionofastrocyticglutamatetransporters,whichmay,inturn,provideneuroprotection.
Productionofstablequininenanodispersionsusingesterifiedγ-polyglutamicacidbiopolymer.
Hoennscheidt,C.,Kreyenschulte,D.,Margaritis,A.&Krull,R.(2013).BiochemicalEngineeringJournal,79,259-266.
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NovelmethodsareneededforthedevelopmentofnanodisperseddrugformulationstoenhancebioavailABIlityofmanyhydrophobicpharmaceuticals.Thepoorlywater-solublequinineisawell-knownanti-malariadrugwhichcanbeusedasapromisingmodelcompoundforthedevelopmentofnovelnanodispersedformulations.Inadditiontohydrophobicdrug"sownaffectingproperties,surfactantsplayanimportantrolefortheenhancementoftheirlowbioavailabilitybypreparingstabledispersions.Amphiphiliccompoundscanefficientlybeusedtostabilizecolloidalfragmentsbypreventingtheprecipitationorcrystallizationofpoorlywater-solubleactiveingredientsduringfabrication.Anovelbiopolymerderivativebasedonthebiotechnologicallyproducedγ-polyglutamicacid(γ-PGA)fromBacilluslicheniformiscultivationwasdevelopedforencapsulationoftheactiveingredient.High-molecularγ-PGAisananionicpolyelectrolytethatwasoptimizedandmodifiedwithhydrophobicL-phenylalanineethylester(L-PAE)toformanamphiphiliccombpolymerP(γ-GA-r-L-PAE)withsurfactiveproperties.Theapproachofthenanodispersionpolymerconcentration,molecularweightandgraftingdegreeenablestheefficientstabilizationofthepoorlywater-solublemodeldrug.Theresearchpresentedinthisreportindicatesthepotentialbenefitsofhydrophobicallymodifiedγ-PGAandsuggestsitspotentialroleinformingstabledispersionsforfuturepharmaceuticalapplications.
Gamma-aminobutyricacid,glutamatedehydrogenaseandglutamatedecarboxylaselevelsinphylogeneticallydivergentplants.
Seher,Y.,Filiz,O.&Melike,B.(2013).PlantSystematicsandEvolution,299(2),403-412.
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Gamma-aminobutyricacid(GABA)isanonproteinaminoacidfoundinawiderangeoforganismsincludingplants.SeveralstudieshaveshownthatGABAplaysdifferentrolesinplantmetabolismincludingcarbon–nitrogenmetabolism,energybalance,signalinganddevelopment.IthasbeensuggestedthattheoccurrenceofGABAandtheenzymesrelatedtoGABAbiosynthesisinprokaryotesandeukaryotesmaybeimportantinevolutionanddiversification.However,studiesofGABAbiosynthesisandGABAlevelsinanevolutionarycontextarerestrictedtosequencedplantgenomes.InthisstudyweaimedtocomparetheactivitiesofGDHandGADenzymesandtotalnitrogen,andthecontentsoftotalsolubleprotein,succinate,glutamate,prolineandGABAinplantsfromdifferentphylogeneticlevelsincludingUlvalactuca,Pseudeverniafurfuracea,Nephrolepsisexaltata,Ginkgobiloba,Pinuspinea,Magnoliagrandiflora,Nymphaeaalba,Urticadioica,Portulacaoleraceae,Malvasylvestris,Rosacanina,Lavandulastoechas,Washingtoniafilifera,AvenabarbataandIriskaempferi.TheactivitiesofGADandGDHenzymesdifferedaccordingtothespeciesandwerenotalwaysparalleltoGABAlevels.ThediscrepancyinthecontentsofsuccinateandGABAbetweenhigherandprimitiveplantswasalsoprominent.Glutamatelevelswerehighwithafewexceptionsandprolinecontentswereatsimilarlowvaluesascomparedtootheraminoacids.OurresultssupportthehypothesisthattheGABAshuntplaysakeyroleincarbonandnitrogenpartitioningvialinkingaminoacidmetabolismandthetricarboxylicacidcyclewhichisessentialforhigherplantspecies.
Metabolicengineeringofthemixed-acidfermentationpathwayofEscherichiacoliforanaerobicproductionofglutamateanditaconate.
Vuoristo,K.S.,Mars,A.E.,Sangra,J.V.,Springer,J.,Eggink,G.,Sanders,J.P.&Weusthuis,R.A.(2015).AMBExpress,5(1),61.
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Itaconicacid,anunsaturatedC5-dicarboxylicacid,isabiobasedbuildingblockforthepolymerindustry.ThepurposeofthisstudywastoestablishproofofprincipleforananaerobicfermentationprocessfortheproductionofitaconicacidbymodificationofthemixedacidfermentationpathwayofE.coli.E.coliBW25113(DE3)andthephosphateacetyltransferase(pta)andlactatedehydrogenase(ldhA)deficientstrainE.coliBW25113(DE3)Δpta-ΔldhAwereusedtostudyanaerobicitaconateproductioninE.coli.Heterologousexpressionofthegeneencodingcis-aconitatedecarboxylase(cadA)fromA.terreusinE.coliBW25113(DE3)didnotresultinitaconateproductionunderanaerobicconditions,but0.08 mMofitaconatewasformedwhenthegenesencodingcitratesynthase(gltA)andaconitase(acnA)fromCoryNEBacteriumglutamicumwerealsoexpressed.ThesameamountwasproducedwhencadAwasexpressedinE.coliBW25113(DE3)Δpta-ΔldhA.Thetitreincreased8timesto0.66 mM(1.2 %Cmol)whenE.coliBW25113(DE3)Δpta-ΔldhAalsoexpressedgltAandacnA.Inaddition,thisstrainproduced8.5 mM(13 %Cmol)ofglutamate.Theuseofanitrogen-limitedgrowthmediumreducedtheaccumulationofglutamatebynearly50 %comparedtothenormalmedium,andalsoresultedinamorethan3-foldincreaseoftheitaconatetitreto2.9 mM.Theseresultsdemonstratedthat E.coli haspotentialtoproduceitaconateandglutamateunderanaerobicconditions,closingtheredoxbalancebyco-productionofsuccinateorethanolwithH2 andCO2.
Genetic,enzymaticandmetaboliteprofilingoftheLactobacilluscaseigrouprevealsstrainbiodiversityandpotentialapplicationsforflavourdiversification.
Stefanovic,E.,Kilcawley,K.N.,Rea,M.C.,Fitzgerald,G.F.&McAuliffe,O.(2017).JournalofAppliedMicroBIOLOGy,122(5),1245-1261.
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Aims:TheLactobacilluscaseigrouprepresentsawidelyexploredgroupoflacticacidbacteria,characterizedbyahighlevelofbiodiversity.Inthisstudy,thegeneticandphenotypicdiversityofacollectionofmorethan300isolatesoftheLact.caseigroupandtheirpotentialtoproducevolatilemetabolitesimportantforflavourdevelopmentindairyproducts,wasexamined.MethodsandResults:Followingconfirmationofspeciesby16SrRNAPCR,thediversityoftheisolateswasdeterminedbypulsed-fieldgelelectrophoresis.Theactivitiesofenzymesinvolvedintheproteolyticcascadewereassessedandsignificantdifferencesamongthestrainswereobserved.Tenstrainswerechosenbasedontheresultsoftheirenzymesactivitiesandtheywereanalysedfortheirabilitytoproducevolatilesinmediawithincreasedconcentrationsofarepresentativearomatic,branchedchainandsulphuraminoacid.Volatileswereassessedusinggaschromatographycoupledwithmassspectrometry.Strain-dependentdifferencesintherangeandtypeofvolatilesproducedwereevident.Conclusions:StrainsoftheLact.caseigrouparecharacterizedbygeneticandmetabolicdiversitywhichsupportsvariabilityinvolatileproduction.SignificanceandImpactoftheStudy:Thisstudyprovidesascreeningapproachfortheknowledge-basedselectionofstrainspotentiallyenablingflavourdiversificationinfermenteddairyproducts.
ColourimetricmethodforthedeterminationofL-GlutamicAcid
(MonosodiumGlutamate;MSG)infoodstuffsandothermaterials
Principle:
(beefliverglutamatedehydrogenase)
(1)L-Glutamicacid+NAD++H2O↔2-oxoglutarate+NADH+NH4+
(diaphorase)
(2)INT+NADH+H+→NAD++INT-formazan
Kitsize: * 60assays(manual)/600(microplate)
/700(auto-analyser)* Thenumberofmanualtestsperkitcanbedoubledifallvolumesarehalved.
ThiscanbereADIlyaccommodatedusingtheMegaQuantTM Wave
Spectrophotometer(D-MQWAVE).Method: Spectrophotometricat492nm
Reactiontime: ~8min
Detectionlimit: 0.21mg/L
Applicationexamples:
Fruitandvegetables(e.g.tomato),processedfruitandvegetables
(e.g.tomatopuree/juice,ketchup,soysauce),condiments,processed
meatproducts(e.g.extracts,bouillonandsausages),soup,pharmaceuticals
andothermaterials(e.g.biologicalcultures,samples,etc.)
Methodrecognition:
MethodsbasedonthisprinciplehavebeenacceptedbyISO,GOST
andNMKL
Advantages
- Verycompetitiveprice(costpertest)
- Allreagentsstablefor>2yearsafterpreparation
- Glutamatedehydrogenasesolutionstableat-20°C
- Nowasteddiaphorasesolution(stablesUSPensionsupplied)
- Rapidreaction
- Mega-Calc™softwaretoolisavailablefromourwebsiteforhassle-freerawdataprocessing
- Standardincluded
- Suitableformanual,microplateandauto-analyserformats
品牌介绍
Megazyme品牌产品简介
来源：作者：人气：2149发表时间：2016-05-19 10:59:00【大 中 小】
Megazyme是一家全球性公司，专注于开发和提供用于饮料、谷物、乳制品、食品、饲料、发酵、生物燃料和葡萄酒产业用的分析试剂、酶和检测试剂盒。Megazyme的许多检测试剂盒产品已经为众多官方科学协会（包括AOAC, AACC , RACI, EBC和ICC等），经过严格的审核，批准认证为官方标准方法，确保以准确、可靠、定量和易于使用的测试方法，满足客户的质量诉求。
Megazyme的主要产品线包括：
◆ 检测试剂盒
◆ 酶
◆ 酶底物
◆ 碳水化合物
◆ 化学品/仪器
官网地址：http://www.megazyme.com
检测试剂盒特色产品：
货号
中文品名
用途
K-ACETAF
乙酸[AF法]检测试剂盒
酶法定量分析乙酸最广泛使用的方法
K-ACHDF
可吸收糖/膳食纤维检测试剂盒
酒精沉淀法测定膳食纤维
K-AMIAR
氨快速检测试剂盒
用于包括葡萄汁、葡萄酒以及其它食品饮料样品中氨含量的快速检测分析。
K-AMYL
直链淀粉/支链淀粉检测试剂盒
谷物淀粉和而粉中直链淀粉/支链淀粉比例和含量检测
K-ARAB
阿拉伯聚糖检测试剂盒
果汁浓缩液中阿拉伯聚糖的检测
K-ASNAM
L-天冬酰胺/L-谷氨酰胺和氨快速检测试剂盒
用于食品工业中丙烯酰胺前体、细胞培养基、以及上清液组分中、L-天冬酰胺，谷氨酰胺和氨的检测分析
K-ASPTM
阿斯巴甜检测试剂盒
专业用于测定饮料和食品中阿斯巴甜含量，操作简单
K-BETA3
β-淀粉酶检测试剂盒
适用于麦芽粉中β-淀粉酶的测定
K-BGLU
混合键β-葡聚糖检测试剂盒
测定谷物、荞麦粉、麦汁、啤酒及其它食品中混合键β-葡聚糖(1,3:1,4-β-D-葡聚糖)的含量
K-CERA
α-淀粉酶检测试剂盒
谷物和发酵液（真菌和细菌）中α-淀粉酶的分析测定
K-CITR
柠檬酸检测试剂盒
快速、可靠地检测食品、饮料和其它物料中柠檬酸（柠檬酸盐）含量
K-DLATE
乳酸快速检测试剂盒
快速、特异性检测饮料、肉类、奶制品和其它食品中L-乳酸和D-乳酸(乳酸盐）含量
K-EBHLG
酵母β-葡聚糖酶检测试剂盒
用于测量和分析酵母中1,3:1,6?-β-葡聚糖，也可以检测1,3-葡聚糖
K-ETSULPH
总亚硫酸检测试剂盒
测定葡萄酒、饮料、食品和其他物料中总亚硫酸含量（按二氧化硫计）的一种简单，高效，可靠的酶法检测方法
K-FRGLMQ
D-果糖/D-葡萄糖[MegaQuant法]检测试剂盒
适用于使用megaquant?色度计（505nm下）测定葡萄、葡萄汁和葡萄酒中D-果糖和D-葡萄糖的含量。
K-FRUC
果聚糖检测试剂盒
含有淀粉、蔗糖和其他糖类的植物提取物和食品中果聚糖的含量测定。
K-FRUGL
D-果糖/D-葡萄糖检测试剂盒
对植物和食品中果糖或葡萄糖含量的酶法紫外分光测定。
K-GALM
半乳甘露聚糖检测试剂盒
食品和植物产品中半乳甘露聚糖的含量检测
K-GLUC
D-葡萄糖[GOPOD]检测试剂盒
谷物提取物中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUHK
D-葡萄糖[HK]检测试剂盒
植物和食品中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUM
葡甘聚糖检测试剂盒
植物和食品中葡甘聚糖的含量测定。
K-INTDF
总膳食纤维检测试剂盒
总膳食纤维特定检测和分析
K-LACGAR
乳糖/D-半乳糖快速检测试剂盒
用于快速检测食品和植物产品中乳糖、D-半乳糖和L-阿拉伯糖
K-LACSU
乳糖/蔗糖/D-葡萄糖检测试剂盒
混合面粉和其它物料中蔗糖、乳糖和D-葡萄糖的测定
K-LACTUL
乳果糖检测试剂盒
特异性、快速和灵敏测量奶基样品中乳果糖含量
K-MANGL
D-甘露糖/D-果糖/D-葡萄糖检测试剂盒
适合测定植物产品和多糖酸性水解产物中D-甘露糖含量
K-MASUG
麦芽糖/蔗糖/D-葡萄糖检测试剂盒
在植物和食品中麦芽糖，蔗糖和葡萄糖的含量检测
K-PECID
胶质识别检测试剂盒
食品配料中果胶的鉴别
K-PHYT
植酸(总磷)检测试剂盒
食品和饲料样品植酸/总磷含量测量的简便方法。不需要通过阴离子交换色谱对植酸纯化，适合于大量样本分析
K-PYRUV
丙酮酸检测试剂盒
在啤酒、葡萄酒、果汁、食品和体液中丙酮酸分析
K-RAFGA
棉子糖/D-半乳糖检测试剂盒
快速测量植物材料和食品中棉子糖和半乳糖含量
K-RAFGL
棉子糖/蔗糖/D-半乳糖检测试剂盒
分析种子和种子粉中D-葡萄糖、蔗糖、棉子糖、水苏糖和毛蕊花糖含量。通过将棉子糖、水苏糖和毛蕊花糖酶解D-葡萄糖、D-果糖和半乳糖，从而测定葡萄糖含量来确定
K-SDAM
淀粉损伤检测试剂盒
谷物面粉中淀粉损伤的检测和分析
K-SUCGL
蔗糖/D-葡萄糖检测试剂盒
饮料、果汁、蜂蜜和食品中蔗糖和葡萄糖的分析
K-SUFRG
蔗糖/D-果糖/D-葡萄糖检测试剂盒
适用于植物和食品中蔗糖、D-葡萄糖和D-果糖的测定
K-TDFR
总膳食纤维检测试剂盒
总膳食纤维检测
K-TREH
海藻糖检测试剂盒
快速、可靠地检测食品、饮料和其它物料中海藻糖含量
K-URAMR
尿素/氨快速检测试剂盒
适用于水、饮料、乳制品和食品中尿素和氨的快速测定
K-URONIC
D-葡萄糖醛酸/D-半乳糖醛酸检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中六元糖醛酸含量（D-葡萄糖醛酸和D-半乳糖醛酸）
K-XYLOSE
D-木糖检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中D-木糖含量
K-YBGL
Beta葡聚糖[酵母和蘑菇]检测试剂盒
检测酵母和蘑菇制品中1,3:1,6-beta-葡聚糖和α-葡聚糖含量
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