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Megazyme/D-Isocitric Acid Assay Kit/K-ISOC/100 assays (manual) / 1000 assays (microplate) / 1000 assays (auto-analyser)
TheD-IsocitricAcidtestkitisfor thespecificandrapidmeasurementandanalysisofD-isocitricacidinfruitjuices.
Suitableformanual,auto-analyserandmicroplateformats.
MetabolicFlexibilityofYarrowialipolyticaGrowingonGlycerol.
UV-methodforthedeterminationofD-IsocitricAcidinfoodstuffs
Principle:
(isocitratedehydrogenase)
(1)D-Isocitricacid+NADP+→2-oxoglutarate+CO2+NADPH+H+
(pH9-10)
(2)D-Isocitricacidester+H2O→D-isocitricacid+alcohol
(pH9-10)
(3)D-Isocitricacidlactone+H2O→D-isocitricacid
Kitsize: * 100assays(manual)/1000(microplate)
/1000(auto-analyser)* Thenumberofmanualtestsperkitcanbedoubledifallvolumesarehalved.
ThiscanbereADIlyaccommodatedusingtheMegaQuantTM Wave
Spectrophotometer(D-MQWAVE).Method: Spectrophotometricat340nm
Reactiontime: ~3min
Detectionlimit: 0.35mg/L
Applicationexamples:
Fruitjuices,fruitproducts,softdrinksandothermaterials(e.g.biological
cultures,samples,etc.)
Methodrecognition:
MethodsbasedonthisprinciplehavebeenacceptedbyEN,NEN,NF,
DIN,GOST,IFUandAIJN
Advantages
- Verycompetitiveprice(costpertest)
- Allreagentsstablefor>2yearsafterpreparation
- Nowastedisocitratedehydrogenasesolution(stablesUSPensionsupplied)
- Veryrapidreaction
- Mega-Calc™softwaretoolisavailablefromourwebsiteforhassle-freerawdataprocessing
- Standardincluded
- Suitableformanual,microplateandauto-analyserformats