Megazyme/L-乳酸（L-乳酸）分析试剂盒/K-LATE/50分析（手动）/500分析（微孔板）/450分析（自动分析仪
商品编号：
K-LATE
品牌：
Megazyme INC
市场价：
¥2200.00
美元价：
1320.00
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
TheL-LacticAcid(L-Lactate)AssayKitisused forthespecificmeasurementandanalysisofL-lacticacid(L-lactate)inbeverages,meat,dairyandfoodproducts.
ExtendedcofactorsstABIlity.Dissolvedcofactorsstablefor>1yearat4oC.
Suitableformanual,auto-analyserandmicroplateformats.
Grapeandwineanalysis:Oenologiststoexploitadvancedtestkits.
Charnock,S.C.&McCleary,B.V.(2005).RevuedesEnology,117,1-5.
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Itiswithoutdoubtthattestingplaysapivotalrolethroughoutthewholeofthevinificationprocess.ToproducethebestpossIBLequalitywineandtominimiseprocessproblemssuchas“stuck”fermentationortroublesomeinfections,itisnowrecognisedthatifpossibletestingshouldbeginpriortoharvestingofthegrapesandcontinuethroughtobottling.TrADItionalmethodsofwineanalysisareoftenexpensive,timeconsuming,requireeitherelaborateequipmentorspecialistexpertiseandfrequentlylackaccuracy.However,enzymaticbio-analysisenablestheaccuratemeasurementofthevastmajorityofanalytesofinteresttothewinemaker,usingjustonepieceofapparatus,thespectrophotometer(seepreviousissueNo.116foradetailedtechnicalreview).Grapejuiceandwineareamenabletoenzymatictestingasbeingliquidstheyarehomogenous,easytomanipulate,andcangenerallybeanalysedwithoutanysamplepreparation.
Megazyme“advanced”winetestkitsgeneralcharacteristicsandvalidation.
Charnock,S.J.,McCleary,B.V.,Daverede,C.&Gallant,P.(2006).ReveuedesOenologues,120,1-5.
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ManyoftheenzymatictestkitsareofficialmethodsofprestigiousorganisationssuchastheAssociationofOfficialAnalyticalChemicals(AOAC)andtheAmericanAssociationofCerealChemists(AACC)inresponsetotheinterestfromoenologists.Megazymedecidedtouseitslonghistoryofenzymaticbio-analysistomakeasignificantcontributiontothewineindustry,bythedevelopmentofarangeofadvancedenzymatictestkits.Thistaskhasnowbeensuccessfullycompletedthroughthestrategicandcomprehensiveprocessofidentifyinglimitationsofexistingenzymaticbio-analysistestkitswheretheyoccurred,andthenusingadvancedtechniques,suchasmolecularBIOLOGy(photo1),torapidlyovercomethem.Noveltestkitshavealsobeendevelopedforanalytesofemerginginteresttotheoenologist,suchasyeastavailablenitrogen(YAN;seepages2-3ofissue117article),orwherepreviouslyenzymesweresimplyeithernotavailable,orweretooexpensivetoemploy,suchasforD-mannitolanalysis.
ProductionofL-lacticacidfromagreenmicroalga,Hydrodictyonreticulum,byLactobacillusparacaseiLA104isolatedfromthetraditionalKoreanfood,makgeolli.
Nguyen,C.M.,Kim,J.S.,Hwang,H.J.,Park,M.S.,Choi,G.J.,Choi,Y.H.,Jang,K.S.&Kim,J.C.(2012).BioresourceTechnology,110,552-559.
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Thefreshwatermicroalga,Hydrodictyonreticulum,thatcontained47.5%reducingsugarsincluding35%glucosewasusedassubstratefortheproductionofL-lacticacid(LA)byLA-producingbacteria.LactobacillusparacaseiLA104wasselectedforfermentationina5-lfermentorsinceitwasabletogrowatpH3,60gLA/l,200gglucose/l,125gNaCl/l,and45°Candproducedover97.3%opticallypureL-lacticacidwithglucoseasasubstrate.SimultaneoussaccharificationandcofermentationfromH.reticulumtoL-LAusingLA104wasinvestigatedinajarfermentor.Theyieldreached46g/100gH.reticulumdrymaterial,withafinalconcentrationof37.11g/landaproductivityof1.03g/l/h.ThisisthefirstreportoftheproductionofL-LAfromamicroalga,andH.reticulumcouldbeapotentialfeedstockforlarge-scaleproductionofL-LAbyLA104.
AnovellacticacidbacteriumfortheproductionofhighpurityL-lacticacid,Lactobacillusparacaseisubsp.paracaseiCHB2121.
Moon,S.K.,Wee,Y.J.&Choi,G.W.(2012).JournalofBioscienceandBioengineering,114(2),155-159.
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Fermentation-derivedlacticacidhasseveralpotentialindustrialusesasanintermediatecarbonchemicalandarawmaterialforbiodegradablepolymer.Wethereforeundertooktheidentificationofanovelbacterialstrainthatiscapableofproducinghighconcentrationsoflacticacidandhaspotentialcommercialapplications.AnovelL(+)-lacticacidproducingbacterium,Lactobacillusparacaseisubsp.paracaseiCHB2121wasisolatedfromsoilobtainednearanethanolproductionfactoryandidentifiedby16SrRNAgenesequenceanalysisandcharacterizationusinganAPI50CHLkit.L.paracaseisubsp.paracaseiCHB2121efficientlyproduced192g/Llacticacidfrommediumcontaining200g/Lofglucose,with3.99g/(L•h)productivity,and0.96g/gyield.Inaddition,theopticalpurityoftheproducedlacticacidwasestimatedtobe96.6%L(+)-lacticacid.ThenewlyidentifiedL.paracaseisubsp.paracaseiCHB2121efficientlyproduceshighconcentrationsoflacticacid,andmaybesuitableforuseintheindustrialproductionoflacticacid.
HistoneacetylationregulatesintracellularpH.
McBrian,M.A.,Behbahan,I.S.,Ferrari,R.,Su,T.,Huang,T.W.,Li,K.,Hong,C.S.,Christofk,H.R.,Vogelauer,M.,Seligson,D.B.&Kurdistani,S.K.(2013).MolecularCell,49(2),310-321.
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Differencesingloballevelsofhistoneacetylationoccurinnormalandcancercells,althoughthereasonwhycellsregulatetheselevelshasbeenunclear.HerewedemonstratearoleforhistoneacetylationinregulatingintracellularpH(pHi).AspHidecreases,histonesaregloballydeacetylatedbyhistonedeacetylases(HDACs),andthereleasedacetateanionsarecoexportedwithprotonsoutofthecellbymonocarboxylatetransporters(MCTs),preventingfurtherreductionsinpHi.Conversely,globalhistoneacetylationincreasesaspHirises,suchaswhenrestingcellsareinducedtoproliferate.InhibitionofHDACsorMCTsdecreasesacetateexportandlowerspHi,particularlycompromisingpHimaintenanceinacidicenvironments.GlobaldeacetylationatlowpHisreflectedatagenomiclevelbydecreasedabundanceandextensiveredistributionofacetylationthroughoutthegenome.Thus,acetylationofchromatinfunctionsasarheostattoregulatepHiwithimportantimplicationsformechanismofactionandtherapeuticuseofHDACinhibitors.
RelationshipbetweenFishSizeandMetabolicRateintheOxyconformingInangaGalaxiasmaculatusRevealsSize-DependentStrategiestoWithstandHypoxia.
Urbina,M.A.&Glover,C.N.(2013).PhysiologicalandBiochemicalZoology,86(6),740-749.
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Therelationshipbetweenmetabolicrateandbodysizeinanimalsisunlikelytobeaconstantbutisinsteadshapedbyavarietyofintrinsic(i.e.,physiological)andextrinsic(i.e.,environmental)factors.Thisstudyexaminedtheeffectofenvironmentaloxygentensiononoxygenconsumptionasafunctionofbodymassinthegalaxiidfish,inanga(Galaxiasmaculatus).Asanoxyconformer,thisfishlacksovertintrinsicregulationofoxygenconsumption,eliminatingthisasafactoraffectingthescalingrelationshipatdifferentoxygentensions.Therelationshipbetweenoxygenconsumptionrateandbodysizewasbestdescribedbyapowerfunction,withanexponentof0.82,higherthanthetheoreticalvaluesof0.66or0.75.ThevalueofthisexponentwassignificantlyalteredbyenvironmentalPo2,firstincreasingasPo2decreasedandthendecliningatthelowestPo2tested.Thesedatasuggestthatthescalingexponentisspeciesspecificandregulatedbyextrinsicfactors.FurThermore,theexternalPo2atwhichfishlostequilibriumwasrelatedtofishsize,aneffectexplainedbythescalingofanaerobiccapacitywithfishmass.Therefore,althoughbiggerfishwereforcedtodepressaerobicmetabolismmorerapidlythansmallfishwhenexposedtoprogressivehypoxia,theywerebetterabletoenactanaerobicmetabolism,potentiallyextendingtheirsurvivalinhypoxia.
ExposuretoelevatedtemperatureandpCO2reducesrespirationrateandenergystatusintheperiwinkleLittorinalittorea.
Melatunan,S.,Calosi,P.,Rundle,S.D.,Moody,A.J.&Widdicombe,S.(2011).PhysiologicalandBiochemicalZoology,84(6),583-594.
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Inthefuture,marineorganismswillfacethechallengeofcopingwithmultipleenvironmentalchangesassociatedwithincreasedlevelsofatmosphericPco2,suchasoceanwarmingandacidification.Topredicthoworganismsmayormaynotmeetthesechallenges,anin-depthunderstandingofthephysiologicalandbiochemicalmechanismsunderpinningorganismalresponsestoclimatechangeisneeded.Here,weinvestigatetheeffectsofelevatedPco2andtemperatureonthewhole-organismandcellularphysiologyoftheperiwinkleLittorinalittorea.Metabolicrates(measuredasrespirationrates),adenylateenergynucleotideconcentrationsandindexes,andend-productmetaboliteconcentrationsweremeasured.Comparedwithvaluesforcontrolconditions,snailsdecreasedtheirrespirationrateby31%inresponsetoelevatedPco2andby15%inresponsetoacombinationofincreasedPco2andtemperature.Decreasedrespirationrateswereassociatedwithmetabolicreductionandanincreaseinend-productmetabolitesinacidifiedtreatments,indicatinganincreasedrelianceonanaerobicmetabolism.TherewasalsoaninteractiveeffectofelevatedPco2andtemperatureontotaladenylatenucleotides,whichwasapparentlycompensatedforbythemaintenanceofadenylateenergychargeviaAMPdeaminaseactivity.Ourfindingssuggestthatmarineintertidalorganismsarelikelytoexhibitcomplexphysiologicalresponsestofutureenvironmentaldrivers,withlikelynegativeeffectsongrowth,populationdynamics,and,ultimately,ecosystemprocesses.
Constructionofafood-gradecellsurfacedisplaysystemforLactobacilluscasei.
Qin,J.,Wang,X.,Kong,J.,Ma,C.&Xu,P.(2014).MicrobiologicalResearch,169(9-10),733-740.
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Inthisstudy,afood-gradecellsurfacedisplayhost/vectorsystemforLactobacilluscaseiwasconstructed.Thefood-gradehostL.caseiQ-5wasalactose-deficientderivativeofL.caseiATCC334obtainedbyplasmidelimination.Thefood-gradecellsurfacedisplayvectorwasconstructedbasedonsafeDNAelementsfromlacticacidbacteriacontainingthefollowing:pSH71repliconfromLactococcuslactis,lactosemetabolismgenesfromL.caseiATCC334ascomplementationMarkers,andsurfacelayerproteingenefromLactobacillusacidophilusATCC4356forcellsurfacedisplay.Thefeasibilityofthenewhost/vectorsystemwasverifiedbytheexpressionofgreenfluorescentprotein(GFP)onL.casei.Laserscanningconfocalmicroscopyandimmunofluorescenceanalysisusinganti-GFPantibodyconfirmedthatGFPwasanchoredonthesurfaceoftherecombinantcells.ThestabilityofrecombinantL.caseicellsinartificialgastrointestinalconditionswasverified,whichisbeneficialfororalvaccinationapplications.Theseresultsindicatethatthefood-gradehost/vectorsystemcanbeanexcellentantigendeliveryvehicleinoralvaccineconstruction.
IdentificationofspoilagemarkermetabolitesinIrishchickenbreastmuscleusingHPLC,GC–MScoupledwithSPMEandtraditionalchemicaltechniques.
Alexandrakis,D.,Brunton,N.P.,Downey,G.&Scannell,A.G.(2012).FoodandBioprocessTechnology,5(5),1917-1923.
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TheaimofthisinvestigationwastodeterminethemetabolitesofspoilagepresentonthesurfaceofIrishchickenbreastmuscleinordertoidentifybiomarkersofmicrobialspoilageandtoverifytheresultsofapreviousstudywhichsuggestedthattheincreaseoffreeaminoacidsisthemainspectralinfluencefactorleadingtothenearandmiddleinfrareddetectionofmicrobialspoilage.Irish-rearedchickenbreastmusclesampleswereindividuallypackedandstoredat4°Cfor8daysunderaerobicconditions.MicrobiologicalanalysisrevealedthatPseudomonasspp.andBrochothrixthermosphactawerethepredominantorganisms(totalviablecounts(TVC),4.24,6.37and8.6colonyformingunity(CFU)g-1fordays0,4and8,respectively,Pseudomonas3.2,5.1andfinally,onday87.4logCFUg-1).GlucoseandL-lactateconcentrationsdecreasedbuttheconcentrationofwater-solublepolypeptidesandaminoacidsincreasedoverstoragetime.HPLCanalysisoffreeaminoacidsrevealedanincreaseofthetotalconcentrationbutthecompositionoftheprofilesdidnotchangeovertime.Headspaceanalysisdetectedthefollowingvolatilecompounds:ethanol,acetone,ethylacetate,methylbenzoate,heptane,C15,C12,methylethylketone,carbondisulphide,dimethylsulphide,hexanal,andtoluene.Ofinterestisthefactthatdetectionofsulphidesandanincreaseofethanol,acetone,andethylacetateconcentrationsoccurredfromday4to8.Theincreaseinfreeaminoacidsthroughoutstorageandtheproductionofvolatilecompoundsafterday4requirefurtherinvestigationbutareselectedaspotentialbiomarkersofmicrobialspoilageastheycouldbeanalyticallydetectedbeforetheacceptedlevelsofsensoryspoilagedetection.
ImprovementoflacticacidproductioninSaccharomycescerevisiaebycellsortingforhighintracellularpH.
Valli,M.,Sauer,M.,Branduardi,P.,Borth,N.,Porro,D.&Mattanovich,D.(2006).AppliedandEnvironmentalMicrobiology,72(8),5492-5499.
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YeaststrainsexpressingheterologousL-lactatedehydrogenasescanproducelacticacid.Althoughthesemicroorganismsaretolerantofacidicenvironments,itisknownthatatlowpH,lacticacidexertsahighlevelofstressonthecells.InthepresentstudyweanalyzedintracellularpH(pHi)andviabilitybystainingwithcSNARF-4Fandethidiumbromide,respectively,oftwolactic-acid-producingstrainsofSaccharomycescerevisiae,CEN.PKm850andCEN.PKRWB876.Theresultsshowedthatthestrainproducingmorelacticacid,CEN.PKm850,hasahigherpHi.Duringbatchculture,weobservedinbothstrainsareductionofthemeanpHiandtheappearanceofasubpopulationofcellswithlowpHi.SimultaneousanalysisofpHiandviabilityprovedthatthecellswithlowpHiweredead.Basedontheobservationthatthebetterlactic-acid-producingstrainhadahigherpHiandthatthecellswithlowpHiweredead,wehypothesizedthatwemightfindbetterlacticacidproducersbyscreeningforcellswithinthehighestpHirange.ThescreeningwasperformedonUV-mutagenizedpopulationsthroughthreeconsecutiveroundsofcellsortinginwhichonlytheviablecellswithinthehighestpHirangewereselected.Theresultsshowedthatlacticacidproductionwassignificantlyimprovedinthemajorityofthemutantsobtainedcomparedtotheparentalstrains.Thebestlactic-acidproducingstrainwasidentifiedwithinthescreeningofCEN.PKm850mutants.
Sourdough-leavenedbreadimprovespostprandialglucoseandinsulinplasmalevelsinsubjectswithimpairedglucosetolerance.
Maioli,M.,Pes,G.M.,Sanna,M.,Cherchi,S.,Dettori,M.,Manca,E.&Farris,G.A.(2008).ActaDiabetologica,45(2),91-96.
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Sourdoughbreadhasbeenreportedtoimproveglucosemetabolisminhealthysubjects.Inthisstudypostprandialglycaemicandinsulinaemicresponseswereevaluatedinsubjectswithimpairedglucosetolerance(IGT)whohadamealcontainingsourdoughbreadleavenedwithlactobacilli,incomparisontoareferencemealcontainingbreadleavenedwithbakeryeast.SixteenIGTsubjects(agerange52–75,averageBMI29.9±4.2kg/m2)wererandomlygivenamealcontainingsourdoughbread(A)andamealcontainingthereferencebread(B)intwoseparateoccasionsatthebeginningofthestudyandafter7days.Sourdoughbreadwasleavenedfor8husingastartercontainingautochthonousSaccharomycescerevisiaeandseveralbacilliabletoproduceasignificantamountofD-andL-lacticacid,whereasthereferencebreadwasleavenedfor2hwithcommercialbakeryeastcontainingSaccharomycescerevisiae.Plasmaglucoseandinsulinlevelsweremeasuredattime0,30,60,120,and180min.InIGTsubjectssourdoughbreadinducedasignificantlylowerplasmaglucoseresponseat30minutes(p=0.048)andasmallerincrementalareaundercurve(AUC)Δ0–30andΔ0–60min(p=0.020and0.018respectively)incomparisontothebreadleavenedwithbakeryeast.Plasmainsulinresponsetothistypeofbreadshowedlowervaluesat30min(p=0.045)andasmallerAUCΔ0–30min(p=0.018).ThisstudyshowsthatinsubjectswithIGTglycaemicandinsulinaemicresponsesaftertheconsumptionofsourdoughbreadarelowerthanafterthebreadleavenedwithbakeryeast.Thiseffectislikelyduetothelacticacidproducedduringdoughleaveningaswellasthereducedavailabilityofsimplecarbohydrates.Thus,sourdoughbreadmaypotentiallybeofbenefitinsubjectswithimpairedglucosemetabolism.
Homo-fermentativeproductionofD-lacticacidbyLactobacillussp.employingcaseinwheypermeateasarawfeed-stock.
Prasad,S.,Srikanth,K.,Limaye,A.M.&Sivaprakasam,S.(2014).BiotechnologyLetters,36(6),1303-1307.
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Caseinwheypermeate(CWP),alactose-enricheddairywasteeffluent,isaviablefeedstockfortheproductionofvalue-addedproducts.TwolacticacidbacteriawerecultivatedinasyntheticcaseinwheypermeatemediumwithorwithoutpHcontrol.LactobacilluslactisATCC4797producedD-lacticacid(DLA)at12.5gl-1inabioreactor.ThevaluesofLeudking–Piretmodelparameterssuggestedthatlactatewasagrowth-associatedproduct.BatchfermentationwasalsoperformedemployingCWP(35glactosel-1)withcaseinhydrolysateasanitrogensupplementinabioreactor.After40h,L.lactisproduced24.3glacticacidl-1withanopticalpurity>98%.ThusCWPmayberegardedasapotentialfeed-stockforDLAproduction.
Assessmentoftheinfluenceofbiocharonrumenandsilagefermentation:Alaboratory-scaleexperiment.
CalveloPereira,R.,Muetzel,S.,Arbestain,M.C.,Bishop,P.,Hina,K.&Hedley,M.(2014).AnimalFeedScienceandTechnology,196,22-31.
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Theadditionofbiochar–charcoalproducedfrompyrolysisofcarbonaceousmaterials–tosoilpresentsseveralchallenges,mainlyassociatedwithitslowbulkdensity,dustinessandtheriskoflosswhenappliedtohillpastures.LivestockcouldbeanadequatevehicleforbiochardeliverytoNewZealandpastoralsoilsviadungpats;however,thepotentialeffectsofbiocharonrumenmetabolismneedtobeinvestigated.Theobjectiveofthisstudywastoinvestigatetheeffectofbiocharadditiontograssbeforeensilingonthefermentationprocessandtotestwhethertheadditionofgrasssilagepreparedwithbiocharorbiochardirectlytohayaffectedtheinvitrorumenfermentation.Thestudyincludedtheuseofdifferenttypesofstartingmaterial(cornstoverandpinewoodchips),twopyrolysistemperatures(350and550°C),post-treatment(additionofdifferenttypesofbio-oilataratioof0.050mL/g),anddifferentdosesofbiochar.Theuseofbiocharfromeithercornstoverorpinepyrolysedat550°Cassilageingredientsatdosesfrom21to186gbiochar/kgdrymatterhadnonegativeeffectonthefinalpropertiesofthesilage,andparticularlyonpH,NH4+-N/totalN,andacetic,N-butyricandL-lacticacidconcentrations.Thesamesilagemixtureswith84and186gbiochar/kgdrymatterwereinvitroincubatedwithbufferedrumenfluid.Therewasabuild-upintotalvolatilefattyacids(VFA)production(P<0.05) in="" the="" presence="" of="" biochar="" –="" increasing="" at="" high="" doses="" –="" irrespective="" of="" the="" type="" of="" starting="" material="" considered.="" this="" increase="" in="" vfa="" was="" also="" observed="" when="" biochar="" were="" added="" to="" hay="" before="">invitroincubation,andwasenhancedwithlow-temperaturebiochar.Noneofthemixturesofbiocharandhayhadanysignificanteffectonmethaneemissionsandammoniareleased.Therewasnoeffectofstartingmaterialtypeorpost-treatmentontheinvitroincubations.Theresultsobtainedinthisresearchdemonstratethelackofnegativeeffectofbiocharmixedwithgrasssilage,orhay,onrumenchemistryduringinvitroincubations.Iflarge-scalestudiesincludinginvivofeedingofcattlewithbiocharconfirmthesefindings,theuseofcattleasadeliverysystemcouldbecomeanovelsolutiontosafelyapplybiochartoNewZealandpastoralsoils.
Behavioural,physiologicalandbiochemicalresponsestoaquatichypoxiainthefreshwatercrayfish,Paranephropszealandicus.
Broughton,R.J.,Marsden,I.D.,Hill,J.V.&Glover,C.N.(2017).ComparativeBiochemistryandPhysiologyPartA:Molecular&IntegrativePhysiology,212,72-80.
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HypoxiaresultingfromaquaticeutrophicationthreatensthepopulationhealthoftheNewZealandfreshwatercrayfish(koura),Paranephropszealandicus.Anintegratedstudy,combiningbehavioural,physiologicalandbiochemicalapproaches,wasthereforeconductedtocharacterisethetoleranceofthisspeciestohypoxia.Whenprovidedwithachoicebetweenwaterflowsofhighorlowdissolvedoxygeninshort-termlaboratoryassays,crayfishdidnotpreferentiallyinhabitwatersofhigherPO2.However,whenanaerialrefugewasprovidedanddissolvedoxygenwasprogressivelydecreased,crayfishemersedataPO2of0.56 ± 0.03 kPa,suggestingarelativelyhightolerancetohypoxia.Closed-boxrespirometrydelineatedaPcrit,thepointatwhichcrayfishtransitionfromoxyregulatingtooxyconforming,of6.0 kPa.SimultaneousmeasurementofheartrateshowednochangesacrossthePO2range.Inresponseto6-hexposurestofixeddissolvedoxygenlevels(normoxia,19.3 kPa;moderatehypoxia,3.5 kPa;andseverehypoxia,1.7 kPa),P.zealandicusshowedahaemolymphPO2thatdeclinedwiththemagnitudeofhypoxia,andwhileplasmapHdeclinedinseverehypoxia,therewerenochangesinplasmaPO2.Plasmaglucoseconcentrationsfell,andplasmalactateincreasedinbothhypoxicgroups.Therewerenochangesintissueglucoseorlactateconcentrations.ThesedataindicatethatP.zealandicusisrelativelytolerantofhypoxia,andpossessesbiochemicalandphysiologicalmechanismsthatfacilitatesurvivalduringshort-termexposurestoacutehypoxia.Ifhypoxiaissevereand/orprolonged,thenthisspeciesiscapableofescapingtoaerialrefugia.
UV-methodforthedeterminationofL-LacticAcidinfoodstuffs,
beveragesandothermaterials
Principle:
(L-lactatedehydrogenase)
(1)L-Lacticacid+NAD+↔pyruvate+NADH+H+
(glutamate-pyruvatetransaminase)
(2)Pyruvate+D-glutamate→D-alanine+2-oxoglutarate
Kitsize: * 50assays(manual)/450(microplate)
/500(auto-analyser)* Thenumberofmanualtestsperkitcanbedoubledifallvolumesarehalved.
ThiscanbereadilyaccommodatedusingtheMegaQuantTM Wave
Spectrophotometer(D-MQWAVE).Method: Spectrophotometricat340nm
Reactiontime: ~10min
Detectionlimit: 0.21mg/L
Applicationexamples:
Wine,beer,softdrinks,milk,dairyproducts(e.g.cream,milk/whey
powder,cheese,condensedmilkandyogurt),foodscontainingmilk
(e.g.dieteticfoods,bakeryproducts,babyfood,chocolate,sweets
andice-cream),egg,eggproducts(e.g.eggpowder),bakingadditives,
vinegar,fruitandvegetables,processedfruitandvegetables
(e.g.tomatoes),meatproducts,foodadditives,feed,paper(and
cardboard),cosmetics,pharmaceuticalsandothermaterials(e.g.biological
cultures,samples,etc.)
Methodrecognition:
MethodsbasedonthisprinciplehavebeenacceptedbyDIN,GOST,
IDF,EEC,EN,ISO,OIV,IFU,AIJNandMEBAK
Advantages
- Verycompetitiveprice(costpertest)
- Allreagentsstablefor>2yearsafterpreparation
- Rapidreaction
- Mega-Calc™softwaretoolisavailablefromourwebsiteforhassle-freerawdataprocessing
- Standardincluded
- Extendedcofactorsstability
- Suitableformanual,microplateandauto-analyserformats
品牌介绍
Megazyme品牌产品简介
来源：作者：人气：2149发表时间：2016-05-19 10:59:00【大 中 小】
Megazyme是一家全球性公司，专注于开发和提供用于饮料、谷物、乳制品、食品、饲料、发酵、生物燃料和葡萄酒产业用的分析试剂、酶和检测试剂盒。Megazyme的许多检测试剂盒产品已经为众多官方科学协会（包括AOAC, AACC , RACI, EBC和ICC等），经过严格的审核，批准认证为官方标准方法，确保以准确、可靠、定量和易于使用的测试方法，满足客户的质量诉求。
Megazyme的主要产品线包括：
◆ 检测试剂盒
◆ 酶
◆ 酶底物
◆ 碳水化合物
◆ 化学品/仪器
官网地址：http://www.megazyme.com
检测试剂盒特色产品：
货号
中文品名
用途
K-ACETAF
乙酸[AF法]检测试剂盒
酶法定量分析乙酸最广泛使用的方法
K-ACHDF
可吸收糖/膳食纤维检测试剂盒
酒精沉淀法测定膳食纤维
K-AMIAR
氨快速检测试剂盒
用于包括葡萄汁、葡萄酒以及其它食品饮料样品中氨含量的快速检测分析。
K-AMYL
直链淀粉/支链淀粉检测试剂盒
谷物淀粉和而粉中直链淀粉/支链淀粉比例和含量检测
K-ARAB
阿拉伯聚糖检测试剂盒
果汁浓缩液中阿拉伯聚糖的检测
K-ASNAM
L-天冬酰胺/L-谷氨酰胺和氨快速检测试剂盒
用于食品工业中丙烯酰胺前体、细胞培养基、以及上清液组分中、L-天冬酰胺，谷氨酰胺和氨的检测分析
K-ASPTM
阿斯巴甜检测试剂盒
专业用于测定饮料和食品中阿斯巴甜含量，操作简单
K-BETA3
β-淀粉酶检测试剂盒
适用于麦芽粉中β-淀粉酶的测定
K-BGLU
混合键β-葡聚糖检测试剂盒
测定谷物、荞麦粉、麦汁、啤酒及其它食品中混合键β-葡聚糖(1,3:1,4-β-D-葡聚糖)的含量
K-CERA
α-淀粉酶检测试剂盒
谷物和发酵液（真菌和细菌）中α-淀粉酶的分析测定
K-CITR
柠檬酸检测试剂盒
快速、可靠地检测食品、饮料和其它物料中柠檬酸（柠檬酸盐）含量
K-DLATE
乳酸快速检测试剂盒
快速、特异性检测饮料、肉类、奶制品和其它食品中L-乳酸和D-乳酸(乳酸盐）含量
K-EBHLG
酵母β-葡聚糖酶检测试剂盒
用于测量和分析酵母中1,3:1,6?-β-葡聚糖，也可以检测1,3-葡聚糖
K-ETSULPH
总亚硫酸检测试剂盒
测定葡萄酒、饮料、食品和其他物料中总亚硫酸含量（按二氧化硫计）的一种简单，高效，可靠的酶法检测方法
K-FRGLMQ
D-果糖/D-葡萄糖[MegaQuant法]检测试剂盒
适用于使用megaquant?色度计（505nm下）测定葡萄、葡萄汁和葡萄酒中D-果糖和D-葡萄糖的含量。
K-FRUC
果聚糖检测试剂盒
含有淀粉、蔗糖和其他糖类的植物提取物和食品中果聚糖的含量测定。
K-FRUGL
D-果糖/D-葡萄糖检测试剂盒
对植物和食品中果糖或葡萄糖含量的酶法紫外分光测定。
K-GALM
半乳甘露聚糖检测试剂盒
食品和植物产品中半乳甘露聚糖的含量检测
K-GLUC
D-葡萄糖[GOPOD]检测试剂盒
谷物提取物中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUHK
D-葡萄糖[HK]检测试剂盒
植物和食品中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUM
葡甘聚糖检测试剂盒
植物和食品中葡甘聚糖的含量测定。
K-INTDF
总膳食纤维检测试剂盒
总膳食纤维特定检测和分析
K-LACGAR
乳糖/D-半乳糖快速检测试剂盒
用于快速检测食品和植物产品中乳糖、D-半乳糖和L-阿拉伯糖
K-LACSU
乳糖/蔗糖/D-葡萄糖检测试剂盒
混合面粉和其它物料中蔗糖、乳糖和D-葡萄糖的测定
K-LACTUL
乳果糖检测试剂盒
特异性、快速和灵敏测量奶基样品中乳果糖含量
K-MANGL
D-甘露糖/D-果糖/D-葡萄糖检测试剂盒
适合测定植物产品和多糖酸性水解产物中D-甘露糖含量
K-MASUG
麦芽糖/蔗糖/D-葡萄糖检测试剂盒
在植物和食品中麦芽糖，蔗糖和葡萄糖的含量检测
K-PECID
胶质识别检测试剂盒
食品配料中果胶的鉴别
K-PHYT
植酸(总磷)检测试剂盒
食品和饲料样品植酸/总磷含量测量的简便方法。不需要通过阴离子交换色谱对植酸纯化，适合于大量样本分析
K-PYRUV
丙酮酸检测试剂盒
在啤酒、葡萄酒、果汁、食品和体液中丙酮酸分析
K-RAFGA
棉子糖/D-半乳糖检测试剂盒
快速测量植物材料和食品中棉子糖和半乳糖含量
K-RAFGL
棉子糖/蔗糖/D-半乳糖检测试剂盒
分析种子和种子粉中D-葡萄糖、蔗糖、棉子糖、水苏糖和毛蕊花糖含量。通过将棉子糖、水苏糖和毛蕊花糖酶解D-葡萄糖、D-果糖和半乳糖，从而测定葡萄糖含量来确定
K-SDAM
淀粉损伤检测试剂盒
谷物面粉中淀粉损伤的检测和分析
K-SUCGL
蔗糖/D-葡萄糖检测试剂盒
饮料、果汁、蜂蜜和食品中蔗糖和葡萄糖的分析
K-SUFRG
蔗糖/D-果糖/D-葡萄糖检测试剂盒
适用于植物和食品中蔗糖、D-葡萄糖和D-果糖的测定
K-TDFR
总膳食纤维检测试剂盒
总膳食纤维检测
K-TREH
海藻糖检测试剂盒
快速、可靠地检测食品、饮料和其它物料中海藻糖含量
K-URAMR
尿素/氨快速检测试剂盒
适用于水、饮料、乳制品和食品中尿素和氨的快速测定
K-URONIC
D-葡萄糖醛酸/D-半乳糖醛酸检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中六元糖醛酸含量（D-葡萄糖醛酸和D-半乳糖醛酸）
K-XYLOSE
D-木糖检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中D-木糖含量
K-YBGL
Beta葡聚糖[酵母和蘑菇]检测试剂盒
检测酵母和蘑菇制品中1,3:1,6-beta-葡聚糖和α-葡聚糖含量
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