TheL-MalicAcid(AnalyserFormat)testkitisananalyserformatforthespecificmeasurementandanalysisofL-malicacid(L-malate)inbeveragesandfoodproducts.Oncalibration,thepreparedreagentislinearto>80microgramsofL-malicacidpermLofassaysolution.
ExtendedcofactorsstABIlity.Dissolvedcofactorsstablefor>1yearat4oC.
Grapeandwineanalysis:Oenologiststoexploitadvancedtestkits.
Megazyme“advanced”winetestkitsgeneralcharacteristicsandvalidation.
Immobilisedyeastgrapemustdeacidificationinarecyclefixedbedreactor.
AnalyserformatUV-methodforthedeterminationofL-MalicAcid
infoodstuffs,beveragesandothermaterials
Principle:
(L-malatedehydrogenase)
(1)L-Malicacid+NAD+↔oxaloacetate+NADH+H+
(glutamate-oxaloacetatetransaminase)
(2)Oxaloacetate+L-glutamate→L-aspartate+2-oxoglutarate
Kitsize: 245.5mLofpreparedreagent(R1+R2)
Method: Spectrophotometricat340nm
Reactiontime: ~3min
Detectionlimit: 20mg/L(recommendedassay)
Applicationexamples:
Wine,beer,fruitjuices,softdrinks,candies,fruitandvegetables,
bread,cosmetics,pharmaceuticalsandothermaterials(e.g.biological
cultures,samples,etc.)
Methodrecognition:
MethodsbasedonthisprinciplehavebeenacceptedbyAOAC,EEC,
EN,NF,NEN,DIN,GOST,OIV,IFU,AIJNandMEBAK
Advantages
- PVPincorporatedtopreventtannininhibition
- Verystablereagentwhenpreparedforauto-analyserapplications
- Linearcalibration(R2~0.9994)upto80μg/mLofL-malicacidinfinalreactionsolution
- ValidatedbytheUniversityofWine,SuzelaRousse,France
- Verycompetitiveprice(costpermLofreagent)
- BothenzymessuppliedasstablesUSPensions
- Veryrapidreaction(~3min)