Megazyme/琥珀酸分析试剂盒/K-SUCC/20分析（手动）/200分析（微孔板）/270分析（自动分析仪）
商品编号：
K-SUCC
品牌：
Megazyme INC
市场价：
¥4152.00
美元价：
2491.20
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
TheSuccinicAcidtestkitissuitableforthespecificassayofsuccinicacidinwine,cheese,eggs,sauceandotherfoodproducts.
ExtendedcofactorsstABIlity.Dissolvedcofactorsstablefor>1yearat4oC.
Suitableformanual,auto-analyserandmicroplateformats.
Grapeandwineanalysis:Oenologiststoexploitadvancedtestkits.
Charnock,S.C.&McCleary,B.V.(2005).RevuedesEnology,117,1-5.
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Itiswithoutdoubtthattestingplaysapivotalrolethroughoutthewholeofthevinificationprocess.ToproducethebestpossIBLequalitywineandtominimiseprocessproblemssuchas“stuck”fermentationortroublesomeinfections,itisnowrecognisedthatifpossibletestingshouldbeginpriortoharvestingofthegrapesandcontinuethroughtobottling.TrADItionalmethodsofwineanalysisareoftenexpensive,timeconsuming,requireeitherelaborateequipmentorspecialistexpertiseandfrequentlylackaccuracy.However,enzymaticbio-analysisenablestheaccuratemeasurementofthevastmajorityofanalytesofinteresttothewinemaker,usingjustonepieceofapparatus,thespectrophotometer(seepreviousissueNo.116foradetailedtechnicalreview).Grapejuiceandwineareamenabletoenzymatictestingasbeingliquidstheyarehomogenous,easytomanipulate,andcangenerallybeanalysedwithoutanysamplepreparation.
Megazyme“advanced”winetestkitsgeneralcharacteristicsandvalidation.
Charnock,S.J.,McCleary,B.V.,Daverede,C.&Gallant,P.(2006).ReveuedesOenologues,120,1-5.
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ManyoftheenzymatictestkitsareofficialmethodsofprestigiousorganisationssuchastheAssociationofOfficialAnalyticalChemicals(AOAC)andtheAmericanAssociationofCerealChemists(AACC)inresponsetotheinterestfromoenologists.Megazymedecidedtouseitslonghistoryofenzymaticbio-analysistomakeasignificantcontributiontothewineindustry,bythedevelopmentofarangeofadvancedenzymatictestkits.Thistaskhasnowbeensuccessfullycompletedthroughthestrategicandcomprehensiveprocessofidentifyinglimitationsofexistingenzymaticbio-analysistestkitswheretheyoccurred,andthenusingadvancedtechniques,suchasmolecularBIOLOGy(photo1),torapidlyovercomethem.Noveltestkitshavealsobeendevelopedforanalytesofemerginginteresttotheoenologist,suchasyeastavailablenitrogen(YAN;seepages2-3ofissue117article),orwherepreviouslyenzymesweresimplyeithernotavailable,orweretooexpensivetoemploy,suchasforD-mannitolanalysis.
DimethylsulfideisanenergysourcefortheheterotrophicmarinebacteriumSagittulastellata.
Boden,R.,Murrell,J.C.&Schäfer,H.(2011).FEMSMicrobiologyLetters,322(2),188-193.
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Dimethylsulfide(DMS)isavolatileorganosulfurcompound,ubiquitousintheoceans,thathasbeencreditedwithvariousrolesinbiogeochemicalcyclingandinclimatecontrol.VariousoceanicsinksofDMSareknown–bothchemicalandbiological–althoughtheyarepoorlyunderstood.InadditiontotheutilizationofDMSasacarbonorasulfursource,someBacteriaareknowntooxidizeittodimethylsulfoxide(DMSO).SagittulastellataisaheterotrophicmemberoftheAlphaproteobacteriafoundinmarineenvironments.IthasbeenshowntooxidizeDMSduringheterotrophicgrowthonsugars,butthereasonsforandthemechanismsofthisoxidationhavenotbeeninvestigated.Here,weshowthattheoxidationofDMStoDMSOiscoupledtoATPsynthesisinS.stellataandthatDMSactsasanenergysourceduringchemoorganoheterotrophicgrowthoftheorganismonfructoseandonsuccinate.DMSdehydrogenase(whichisresponsiblefortheoxidationofDMStoDMSOinothermarineBacteria)andDMSOreductaseactivitieswereabsentfromcellsgrowninthepresenceofDMS,indicatinganalternativerouteofDMSoxidationinthisorganism.
Artefactsincellculture:α-Ketoglutaratecanscavengehydrogenperoxidegeneratedbyascorbateandepigallocatechingallateincellculturemedia.
Long,L.H.&Halliwell,B.(2011).BiochemicalandBiophysicalResearchCommunications,406(1),20-24.
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Ascorbateandseveralphenoliccompoundsreadilyoxidiseincellculturemediatogeneratehydrogenperoxide.However,additionofα-ketoglutarate,whichisknowntobereleasedbyseveralcelltypes,decreasedthelevelsofH2O2,andtheα-ketoglutaratewasdepletedandconvertedtosuccinate.Theseobservationscouldaccountforpreviousreportsoftheprotectiveeffectsofα-ketoglutarateinpromotingthegrowthofcellsinculture,andmaycontributetoexplainingsomeofthevariabilityintheliteratureinreportedratesofH2O2productionfromautoxidisablecompoundsincellculturesystems.
BiochemicalmechanismonGABAaccumulationduringfruitdevelopmentintomato.
Akihiro,T.,Koike,S.,Tani,R.,Tominaga,T.,Watanabe,S.,Iijima,Y.,Aoki,K.,Shibata,D.,Ashihara,H.,Matsukura,C.,Akama,K.,Fujimura,T.&Ezura,H.(2008).PlantandCellPhysiology,49(9),1378-1389.
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Alargeamountofγ-aminobutyricacid(GABA)wasfoundtoaccumulateintomato(Solanumlycopersicum)fruitsbeforethebreakerstage.Shortlythereafter,GABAwasrapidlycatabolizedafterthebreakerstage.WescreenedtheGABA-richtomatocultivar‘DG03-9’whichdidnotshowrapidGABAcatabolismafterthebreakerstage.AlthoughGABAhyperaccumulationandrapidcatabolisminfruitsiswellknown,themechanismsarenotclearlyunderstood.Inordertoclarifythesemechanisms,weperformedcomparativestudiesof‘Micro-Tom’and‘DG03-9’fruitsfortheanalysisofgeneexpressionlevels,proteinlevelsandenzymaticactivitylevelsofGABAbiosynthesis-andcatabolism-relatedenzymes.DuringGABAaccumulation,wefoundpositivecorrelationsamongGABAcontentsandexpressionlevelsofSlGAD2andSlGAD3.Bothofthesegenesencodeglutamatedecarboxylase(GAD)whichisakeyenzymeofGABAbiosynthesis.DuringGABAcatabolism,wefoundastrongcorrelationbetweenGABAcontentsandenzymeactivityofα-ketoglutarate-dependentGABAtransaminase(GABA-TK).Thecontentsofglutamateandaspartate,whicharesynthesizedfromGABAandglutamate,respectively,increasedwithelevationofGABA-TKenzymaticactivity.GABA-TKisthemajorGABAtransaminaseforminanimalsandappearstobeaminorforminplants.In‘DG03-9’fruits,GADenzymaticactivitywasprolongeduntiltheripeningstage,andGABA-TKactivitywassignificantlylow.Takentogether,ourresultssuggestthatGADandGABA-TKplaycrucialrolesinGABAaccumulationandcatabolism,respectively,intomatofruits.
IsolationandcharacterizationofanovelheterotrophicnitrifyingandaerobicdenitrifyingbacteriumPseudomonasstutzeriKTBforbioremediationofwastewater.
Zhou,M.,Ye,H.&Zhao,X.(2014).BiotechnologyandBioprocessEngineering,19(2),231-238.
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Anovelheterotrophicnitrifyingandaerobicdenitrifyingbacterium,KTB,wasisolatedfromactivatedsludgefloccicollectedfromabiologicalaeratedfilteraccordingtothemodifiedTakayamethodandidentifiedasPseudomonasstutzeriby16SrDNAgenesequenceanalysis.Whenshaking-culturedinthepresenceof4.331mmol/Lofnitrate,4.511mmol/Lofnitriteand4.438mmol/Lofammonium,thestraingrewfast,withµmaxbeing0.42,0.45,and0.56/h,anddisplayedhighnitrogenremovalefficiency,withnitrogenremovalratebeing0.239,0.362,and0.361mmol/L/handnitrogenremovalratiobeing99.1,100.0,and100.0%in18h,respectively.Theremovalmainlyoccurredinthelogarithmicphase.Nitriteaccumulationdidnotaffectdenitrificationperformance.Nitrateconcentrationwasbelowthedetectablelimitduringthewholegrowthcyclewhenammoniumwasusedassolenitrogensource.IttoleratedhighDOlevelandexhibitedexcellentaggregationability.Apossiblepathwayinvolvedinthenitrogenremovalprocess,whichdemonstratedafullnitrificationanddenitrificationroute,wasspeculated.Thestrainmightbeagreatcandidateforbiologicalremovalofnitrogencompoundsfromwastewater.
IdentificationofOrganicAcidsinWineThatStimulateMechanismsofGastricAcidSecretion.
Liszt,K.I.,Walker,J.&Somoza,V.(2012).JournalofAgriculturalandFoodChemistry,60(28),7022-7030.
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Winemaycausestomachirritationduetoitsstimulatoryeffectongastricacidsecretion,althoughthemechanismsbywhichwineorcomponentsthereofactivatepathwaysofgastricacidsecretionarepoorlyunderstood.GastricpHwasmeasuredwithanoninvasiveintragastricprobe,demonstratingthatadmiNISTrationof125mLofwhiteorredwinetohealthyvolunteersstimulatedgastricacidsecretionmorepotentlythantheadministrationofequivalentamountsofethanol.Betweenbothbeverages,redwineshowedacleartrendforbeingmoreactiveinstimulatinggastricacidsecretionthanwhitewine(p=0.054).Quantificationoftheintracellularprotonconcentrationinhumangastrictumorcells(HGT-1),awell-establishedindicatorofprotonsecretionand,inturn,stomachacidformationinvivo,confirmedthestrongereffectofredwineascomparedtowhitewine.RT-qPCRexperimentsoncellsexposedtoredwinealsorevealedamorepronouncedeffectthanwhitewineonthefoldchangeexpressionofgenesassociatedwithgastricacidsecretion.Ofthequantitativelyabundantorganicacidsinwine,malicacidandsuccinicacidmostactivelystimulatedprotonsecretioninvitro.However,additionofethanoltoindividualorganicacidsattenuatedthesecretoryeffectoftartaricacid,butnotthatoftheotherorganicacids.Itwasconcludedthatmalicacidforwhitewineandsuccinicacidforredwinearekeyorganicacidsthatcontributetogastricacidstimulation.
SuccinicacidproductionfromorangepeelandwheatstrawbybatchfermentationsofFibrobactersuccinogenesS85.
Li,Q.,Siles,J.A.&Thompson,I.P.(2010).AppliedMicrobiologyandBiotechnology,88(3),671-678.
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Succinicacidisaplatformmoleculethathasrecentlygeneratedconsiderableinterests.Productionofsuccinatefromwasteorangepeelandwheatstrawbyconsolidatedbioprocessingthatcombinescellulosehydrolysisandsugarfermentation,usingacellulolyticbacterium,FibrobactersuccinogenesS85,wasstudied.OrangepeelcontainsD-limonene,whichisawell-knownantibacterialagent.ItseffectsonbatchculturesofF.succinogenesS85wereexamined.Theminimalconcentrationsoflimonenefoundtoinhibitsuccinateandacetategenerationandbacterialgrowthwere0.01%,0.1%,and0.06%(v/v),respectively.Bothpre-treatedorangepeelbysteamdistillationtoremoveD-limoneneandintactwheatstrawwereusedasfeedstocks.Increasingthesubstrateconcentrationsofbothfeedstocks,from5to60g/L,elevatedsuccinateconcentrationandproductivitybutloweredtheyield.Inaddition,pre-treatedorangepeelgeneratedgreatersuccinateproductivitiesthanwheatstrawbuthadsimilarresultanttitres.Thegreatestsuccinatetitreswere1.9and2.0g/Lforpre-treatedorangepeelandwheatstraw,respectively.Thisworkdemonstratedthatagriculturalwastesuchaswheatstrawandorangepeelcanbebiotransformedtosuccinicacidbyaone-stepconsolidatedbioprocessing.Measurestoincreasefermentationefficiencyarealsodiscussed.
Gamma-aminobutyricacid,glutamatedehydrogenaseandglutamatedecarboxylaselevelsinphylogeneticallydivergentplants.
Seher,Y.,Filiz,O.&Melike,B.(2013).PlantSystematicsandEvolution,299(2),403-412.
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Gamma-aminobutyricacid(GABA)isanonproteinaminoacidfoundinawiderangeoforganismsincludingplants.SeveralstudieshaveshownthatGABAplaysdifferentrolesinplantmetabolismincludingcarbon–nitrogenmetabolism,energybalance,signalinganddevelopment.IthasbeensuggestedthattheoccurrenceofGABAandtheenzymesrelatedtoGABAbiosynthesisinprokaryotesandeukaryotesmaybeimportantinevolutionanddiversification.However,studiesofGABAbiosynthesisandGABAlevelsinanevolutionarycontextarerestrictedtosequencedplantgenomes.InthisstudyweaimedtocomparetheactivitiesofGDHandGADenzymesandtotalnitrogen,andthecontentsoftotalsolubleprotein,succinate,glutamate,prolineandGABAinplantsfromdifferentphylogeneticlevelsincludingUlvalactuca,Pseudeverniafurfuracea,Nephrolepsisexaltata,Ginkgobiloba,Pinuspinea,Magnoliagrandiflora,Nymphaeaalba,Urticadioica,Portulacaoleraceae,Malvasylvestris,Rosacanina,Lavandulastoechas,Washingtoniafilifera,AvenabarbataandIriskaempferi.TheactivitiesofGADandGDHenzymesdifferedaccordingtothespeciesandwerenotalwaysparalleltoGABAlevels.ThediscrepancyinthecontentsofsuccinateandGABAbetweenhigherandprimitiveplantswasalsoprominent.Glutamatelevelswerehighwithafewexceptionsandprolinecontentswereatsimilarlowvaluesascomparedtootheraminoacids.OurresultssupportthehypothesisthattheGABAshuntplaysakeyroleincarbonandnitrogenpartitioningvialinkingaminoacidmetabolismandthetricarboxylicacidcyclewhichisessentialforhigherplantspecies.
ExposuretoelevatedtemperatureandPco2reducesrespirationrateandenergystatusintheperiwinkleLittorinalittorea.
Melatunan,S.,Calosi,P.,Rundle,S.D.,Moody,A.J.&Widdicombe,S.(2011).PhysiologicalandBiochemicalZoology,84(6),583-594.
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Inthefuture,marineorganismswillfacethechallengeofcopingwithmultipleenvironmentalchangesassociatedwithincreasedlevelsofatmosphericPco2,suchasoceanwarmingandacidification.Topredicthoworganismsmayormaynotmeetthesechallenges,anin-depthunderstandingofthephysiologicalandbiochemicalmechanismsunderpinningorganismalresponsestoclimatechangeisneeded.Here,weinvestigatetheeffectsofelevatedPco2andtemperatureonthewhole-organismandcellularphysiologyoftheperiwinkleLittorinalittorea.Metabolicrates(measuredasrespirationrates),adenylateenergynucleotideconcentrationsandindexes,andend-productmetaboliteconcentrationsweremeasured.Comparedwithvaluesforcontrolconditions,snailsdecreasedtheirrespirationrateby31%inresponsetoelevatedPco2andby15%inresponsetoacombinationofincreasedPco2andtemperature.Decreasedrespirationrateswereassociatedwithmetabolicreductionandanincreaseinend-productmetabolitesinacidifiedtreatments,indicatinganincreasedrelianceonanaerobicmetabolism.TherewasalsoaninteractiveeffectofelevatedPco2andtemperatureontotaladenylatenucleotides,whichwasapparentlycompensatedforbythemaintenanceofadenylateenergychargeviaAMPdeaminaseactivity.Ourfindingssuggestthatmarineintertidalorganismsarelikelytoexhibitcomplexphysiologicalresponsestofutureenvironmentaldrivers,withlikelynegativeeffectsongrowth,populationdynamics,and,ultimately,ecosystemprocesses.
Identifyingandassessingtheimpactofwineacid-relatedgenesinyeast.
Chidi,B.S.,Rossouw,D.&Bauer,F.F.(2016).Currentgenetics,62(1),149-164.
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Saccharomycescerevisiaestrainsusedforwinemakingshowawiderangeoffermentationphenotypes,andthegeneticbackgroundofindividualstrainscontributessignificantlytotheorganolepticpropertiesofwine.Thisstrain-dependentimpactextendstotheorganicacidcompositionofthewine,animportantqualityparameter.However,littleisknownaboutthegeneswhichmayimpactonorganicacidsduringgrapemustfermentation.Togeneratenovelinsightsintothegeneticregulationofthismetabolicnetwork,asubsetofgeneswasidentifiedbasedonacomparativeanalysisofthetranscriptomesandorganicacidprofilesofdifferentyeaststrainsshowingdifferentproductionlevelsoforganicacids.Thesegenesshowedsignificantinter-straindifferencesintheirtranscriptionlevelsatoneormorestagesoffermentationandwerealsoconsideredlikelytoinfluenceorganicacidmetabolismbasedonexistingfunctionalannotations.GenesselectedinthismannerwereADH3,AAD6,SER33,ICL1,GLY1,SFC1,SER1,KGD1,AGX1,OSM1andGPD2.Yeaststrainscarryingdeletionsforthesegeneswereusedtoconductfermentationsanddetermineorganicacidlevelsatvariousstagesofalcoholicfermentationinsyntheticgrapemust.Theimpactofthesedeletionsonorganicacidprofileswasquantified,leadingtonovelinsightsandhypothesisgenerationregardingtherole/softhesegenesinwineyeastacidmetabolismunderfermentativeconditions.Overall,thedatacontributetoourunderstandingoftherolesofselectedgenesinyeastmetabolismingeneralandoforganicacidmetabolisminparticular.
PseudomonasputidaKT2440strainmetabolizesglucosethroughacycleformedbyenzymesoftheEntner-Doudoroff,EmBDen-Meyerhof-Parnas,andpentosephosphatepathways.
Nikel,P.I.,Chavarría,M.,Fuhrer,T.,Sauer,U.&deLorenzo,V.(2015).JournalofBiologicalChemistry,290(43),25920-25932.
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ThesoilbacteriumPseudomonasputidaKT2440lacksafunctionalEmbden-Meyerhof-Parnas(EMP)pathway,andglycolysisisknowntoproceedalmostexclusivelythroughtheEntner-Doudoroff(ED)route.Toinvestigatetheraisond"êtreofthismetabolicarrangement,thedistributionofperiplasmicandcytoplasmiccarbonfluxeswasstudiedinglucoseculturesofthisbacteriumbyusing13C-labeledsubstrates,combinedwithquantitativephysiologyexperiments,metabolitequantification,andinvitroenzymaticassaysunderbothsaturatingandnon-saturating,quasiinvivoconditions.Metabolicfluxanalysisdemonstratedthat90%oftheconsumedsugarwasconvertedintogluconate,enteringcentralcarbonmetabolismas6-phosphogluconateandfurtherchanneledintotheEDpathway.Remarkably,about10%ofthetriosephosphateswerefoundtoberecycledbacktoformhexosephosphates.ThissetofreactionsmergesactivitiesbelongingtotheED,theEMP(operatinginagluconeogenicfashion),andthepentosephosphatepathwaystoformanunforeseenmetabolicarchitecture(EDEMPcycle).DeterminationoftheNADPHbalancerevealedthatthedefaultmetabolicstateofP.putidaKT2440ischaracterizedbyaslightcatabolicoverproductionofreducingpower.CellsgrowingonglucosethusrunabiochemicalcyclethatfavorsNADPHformation.BecauseNADPHisrequirednotonlyforanabolicfunctionsbutalsoforcounteractingdifferenttypesofenvironmentalstress,suchacyclicoperationmaycontributetothephysiologicalheftinessofthisbacteriuminitsnaturalhabitats.
SUCNR1-mediatedchemotaxisofmacrophagesaggravatesobesity-inducedinflammationanddiabetes.
vanDiepen,J.A.,Robben,J.H.,Hooiveld,G.J.,Carmone,C.,Alsady,M.,Boutens,L.,Bekkenkamp-Grovenstein,M.B.,Hijmans,A.,Engelke,U.F.H.,Wevers,R.A.,Netea,M.G.,Tack,C.J.,Stienstra,R.&Deen,P.M.T.(2017).Diabetologia,1-10.
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Aims/hypothesis:Obesityinducesmacrophagestodriveinflammationinadiposetissue,acrucialsteptowardsthedevelopmentoftype2diabetes.Thetricarboxylicacid(TCA)cycleintermediatesuccinateisreleasedfromcellsundermetabolicstressandhasrecentlyemergedasametabolicsignalinducedbyproinflammatorystimuli.Wethereforeinvestigatedwhethersuccinatereceptor1(SUCNR1)couldplayaroleinthedevelopmentofadiposetissueinflammationandtype2diabetes.Methods:Succinatelevelsweredeterminedinhumanplasmasamplesfromindividualswithtype2diabetesandnon-diabeticparticipants.Succinatereleasefromadiposetissueexplantswasstudied.Sucnr1-/-andwild-type(WT)littermatemicewerefedahigh-fatdiet(HFD)orlow-fatdiet(LFD)for16 weeks.Serummetabolicvariables,adiposetissueinflammation,macrophagemigrationandglucosetoleranceweredetermined.Results:Weshowthathypoxiaandhyperglycaemiaindependentlydrivethereleaseofsuccinatefrommouseadiposetissue(17-foldandupto18-fold,respectively)andthatplasmalevelsofsuccinatewerehigherinparticipantswithtype2diabetescomparedwithnon-diabeticindividuals(+53%; p < 0.01).Sucnr1-/-micehadsignificantlyreducednumbersofmacrophages(0.56 ± 0.07vs0.92 ± 0.15 F4/80cells/adipocytes, p < 0.05)andcrown-likestructures(0.06 ± 0.02vs0.14 ± 0.02,CLS/adipocytes p < 0.01)in=""adipose=""tissue=""and=""significantly=""improved=""glucose=""tolerance="">p < 0.001)compared=""with=""wt=""mice=""fed=""an=""hfd,=""despite=""similarly=""increased=""body=""weights.=""consistently,=""macrophages=""from="">Sucnr1-/-miceshowedreducedchemotaxistowardsmediumcollectedfromapoptoticandhypoxicadipocytes(−59%; p < 0.05).Conclusions/interpretation:OurresultsrevealthatactivationofSUCNR1inmacrophagesisimportantforbothinfiltrationandinflammationofadiposetissueinobesity,andsuggestthatSUCNR1isapromisingtherapeutictargetinobesity-inducedtype2diabetes.
BistabilityandNonmonotonicInductionofthelacOperonintheNaturalLactoseUptakeSystem.
Zander,D.,Samaga,D.,Straube,R.&Bettenbrock,K.(2017).BiophysicalJournal,112(9),1984-1996.
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TheEscherichiacolilacoperonisregulatedbyapositivefeedbackloopwhosepotentialtogenerateanall-or-noneresponseinsinglecellshasbeenaparadigmforbistablegeneexpression.However,sofarbistablelacinductionhasonlybeenobservedusinggratuitousinducers,raisingthequestionaboutthebiologicalrelevanceofbistablelacinductioninthenaturalsettingwithlactoseastheinducer.Infact,theexistingexperimentalevidencepointstoagradedratherthananall-or-noneresponseinthenaturallactoseuptakesystem.Incontrast,predictionsbasedoncomputationalmodelsofthelactoseuptakepathwayremaincontroversial.Althoughsomeargueinfavorofbistability,othersargueagainstit.Here,wereinvestigate lac operonexpressioninsinglecellsusingacombinedexperimental/modelingapproach.Tothisend,weparameterizeawell-supportedmathematicalmodelusingtransientmeasurementsofLacZactivityuponinductionwithdifferentamountsoflactose.Theresultingmodelpredictsamonostableinductioncurveforthewild-typesystem,butindicatesthatoverexpressionoftheLacIrepressorwoulddrivethesystemintothebistableregime.Bothpredictionswereconfirmedexperimentallysupportingtheviewthatthewild-typelacinductioncircuitgeneratesagradedresponseratherthanbistability.Moreinterestingly,wefindthatthelacinductioncurveexhibitsapronouncedmaximumatintermediatelactoseconcentrations.Supportedbyourdata,amodel-basedanalysissuggeststhatthenonmonotonicresponseresultsfromsaturationoftheLacIrepressoratlowinducerconcentrationsanddilutionofLacenzymesduetoanincreasedgrowthratebeyondthesaturationpoint.WespeculatethattheobservedmaximuminthelacexpressionlevelhelpstosavecellularresourcesbylimitingLacenzymeexpressionathighinducerconcentrations.
Partialcharacterizationofphylogeny,ecologyandfunctionofthefibrolyticbacteriumRuminococcusflavefaciensOS14,newlyisolatedfromtherumenofswampbuffalo.
Boonsaen,P.,Kinjo,M.,Sawanon,S.,Suzuki,Y.,Koike,S.&Kobayashi,Y.(2017).AnimalScienceJournal,InPress.
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ThefibrolyticrumenbacteriumRuminococcusflavefaciensOS14wasisolatedfromswampbuffaloanditsphylogenetic,ecologicalanddigestivepropertieswerepartiallycharacterized.Isolatesfromrumencontentsoffourswampbuffalowerescreenedforfibrolyticbacteria;oneofthe40isolatesshowedadistinctivefeatureofsolubilizingcellulosepowderinliquidcultureandwasidentifiedasR.flavefaciensbasedonits16SribosomalDNAsequence.Thisisolate,OS14,wasemployedfordetectionanddigestionstudies,forwhichaquantitativePCRassaywasdevelopedanddefinedculturesweretestedwithrepresentativeforagesinThailand.OS14wasphylogeneticallydistantfromotherisolatedandunculturedR.flavefaciensandshowedlimiteddistributionamongThairuminantsbutwasabsentinJapanesecattle.OS14digestedricestrawandothertropicalforagetoagreaterextentthanthetypestrainC94ofR.flavefaciens.OS14producedmorelactatethanC94,anddigestedparagrasstoproducepropionatemoreextensivelyinco-culturewithlactate-utilizingSelenomonasruminantiumS137thanaco-cultureofC94withS137.TheseresultsindicatethatphylogeneticallydistinctOS14coulddigestThailocalforagemoreefficientlythanthetypestrain,possiblyformingasymbioticcross-feedingrelationshipwithlactate-utilizingbacteria.Thisstrainmightbeusefulforfutureanimalandotherindustrialapplications.
UV-methodforthedeterminationofSuccinicAcidinfoodstuffs,
feed,wineandothermaterials
Principle:
(succinyl-CoAsynthetase)
(1)Succinate+ATP+CoA→ADP+succinyl-CoA+Pi
(pyruvatekinase)
(2)ADP+PEP→ATP+pyruvate
(L-lactatedehydrogenase)
(3)Pyruvate+NADH+H+→NAD++L-lactate
Kitsize: *20assays(manual)/200(microplate)
/270(auto-analyser)* Thenumberofmanualtestsperkitcanbedoubledifallvolumesarehalved.
ThiscanbereadilyaccommodatedusingtheMegaQuantTM Wave
Spectrophotometer(D-MQWAVE).Method: Spectrophotometricat340nm
Reactiontime: ~6min
Detectionlimit: 0.26mg/L
Applicationexamples:
Wine,fruitandvegetables,soysauce,cheese,egg,eggproducts
andothermaterials(e.g.biologicalcultures,samples,etc.)
Methodrecognition:
MethodsbasedonthisprinciplehavebeenacceptedbyEEC
Advantages
- Verycompetitiveprice(costpertest)
- Allreagentsstablefor>2yearsassupplied
- Veryrapidreaction(evenatroomtemperature)
- Mega-Calc™softwaretoolisavailablefromourwebsiteforhassle-freerawdataprocessing
- Standardincluded
- Extendedcofactorsstability
- Suitableformanual,microplateandauto-analyserformats
品牌介绍
Megazyme品牌产品简介
来源：作者：人气：2149发表时间：2016-05-19 10:59:00【大 中 小】
Megazyme是一家全球性公司，专注于开发和提供用于饮料、谷物、乳制品、食品、饲料、发酵、生物燃料和葡萄酒产业用的分析试剂、酶和检测试剂盒。Megazyme的许多检测试剂盒产品已经为众多官方科学协会（包括AOAC, AACC , RACI, EBC和ICC等），经过严格的审核，批准认证为官方标准方法，确保以准确、可靠、定量和易于使用的测试方法，满足客户的质量诉求。
Megazyme的主要产品线包括：
◆ 检测试剂盒
◆ 酶
◆ 酶底物
◆ 碳水化合物
◆ 化学品/仪器
官网地址：http://www.megazyme.com
检测试剂盒特色产品：
货号
中文品名
用途
K-ACETAF
乙酸[AF法]检测试剂盒
酶法定量分析乙酸最广泛使用的方法
K-ACHDF
可吸收糖/膳食纤维检测试剂盒
酒精沉淀法测定膳食纤维
K-AMIAR
氨快速检测试剂盒
用于包括葡萄汁、葡萄酒以及其它食品饮料样品中氨含量的快速检测分析。
K-AMYL
直链淀粉/支链淀粉检测试剂盒
谷物淀粉和而粉中直链淀粉/支链淀粉比例和含量检测
K-ARAB
阿拉伯聚糖检测试剂盒
果汁浓缩液中阿拉伯聚糖的检测
K-ASNAM
L-天冬酰胺/L-谷氨酰胺和氨快速检测试剂盒
用于食品工业中丙烯酰胺前体、细胞培养基、以及上清液组分中、L-天冬酰胺，谷氨酰胺和氨的检测分析
K-ASPTM
阿斯巴甜检测试剂盒
专业用于测定饮料和食品中阿斯巴甜含量，操作简单
K-BETA3
β-淀粉酶检测试剂盒
适用于麦芽粉中β-淀粉酶的测定
K-BGLU
混合键β-葡聚糖检测试剂盒
测定谷物、荞麦粉、麦汁、啤酒及其它食品中混合键β-葡聚糖(1,3:1,4-β-D-葡聚糖)的含量
K-CERA
α-淀粉酶检测试剂盒
谷物和发酵液（真菌和细菌）中α-淀粉酶的分析测定
K-CITR
柠檬酸检测试剂盒
快速、可靠地检测食品、饮料和其它物料中柠檬酸（柠檬酸盐）含量
K-DLATE
乳酸快速检测试剂盒
快速、特异性检测饮料、肉类、奶制品和其它食品中L-乳酸和D-乳酸(乳酸盐）含量
K-EBHLG
酵母β-葡聚糖酶检测试剂盒
用于测量和分析酵母中1,3:1,6?-β-葡聚糖，也可以检测1,3-葡聚糖
K-ETSULPH
总亚硫酸检测试剂盒
测定葡萄酒、饮料、食品和其他物料中总亚硫酸含量（按二氧化硫计）的一种简单，高效，可靠的酶法检测方法
K-FRGLMQ
D-果糖/D-葡萄糖[MegaQuant法]检测试剂盒
适用于使用megaquant?色度计（505nm下）测定葡萄、葡萄汁和葡萄酒中D-果糖和D-葡萄糖的含量。
K-FRUC
果聚糖检测试剂盒
含有淀粉、蔗糖和其他糖类的植物提取物和食品中果聚糖的含量测定。
K-FRUGL
D-果糖/D-葡萄糖检测试剂盒
对植物和食品中果糖或葡萄糖含量的酶法紫外分光测定。
K-GALM
半乳甘露聚糖检测试剂盒
食品和植物产品中半乳甘露聚糖的含量检测
K-GLUC
D-葡萄糖[GOPOD]检测试剂盒
谷物提取物中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUHK
D-葡萄糖[HK]检测试剂盒
植物和食品中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUM
葡甘聚糖检测试剂盒
植物和食品中葡甘聚糖的含量测定。
K-INTDF
总膳食纤维检测试剂盒
总膳食纤维特定检测和分析
K-LACGAR
乳糖/D-半乳糖快速检测试剂盒
用于快速检测食品和植物产品中乳糖、D-半乳糖和L-阿拉伯糖
K-LACSU
乳糖/蔗糖/D-葡萄糖检测试剂盒
混合面粉和其它物料中蔗糖、乳糖和D-葡萄糖的测定
K-LACTUL
乳果糖检测试剂盒
特异性、快速和灵敏测量奶基样品中乳果糖含量
K-MANGL
D-甘露糖/D-果糖/D-葡萄糖检测试剂盒
适合测定植物产品和多糖酸性水解产物中D-甘露糖含量
K-MASUG
麦芽糖/蔗糖/D-葡萄糖检测试剂盒
在植物和食品中麦芽糖，蔗糖和葡萄糖的含量检测
K-PECID
胶质识别检测试剂盒
食品配料中果胶的鉴别
K-PHYT
植酸(总磷)检测试剂盒
食品和饲料样品植酸/总磷含量测量的简便方法。不需要通过阴离子交换色谱对植酸纯化，适合于大量样本分析
K-PYRUV
丙酮酸检测试剂盒
在啤酒、葡萄酒、果汁、食品和体液中丙酮酸分析
K-RAFGA
棉子糖/D-半乳糖检测试剂盒
快速测量植物材料和食品中棉子糖和半乳糖含量
K-RAFGL
棉子糖/蔗糖/D-半乳糖检测试剂盒
分析种子和种子粉中D-葡萄糖、蔗糖、棉子糖、水苏糖和毛蕊花糖含量。通过将棉子糖、水苏糖和毛蕊花糖酶解D-葡萄糖、D-果糖和半乳糖，从而测定葡萄糖含量来确定
K-SDAM
淀粉损伤检测试剂盒
谷物面粉中淀粉损伤的检测和分析
K-SUCGL
蔗糖/D-葡萄糖检测试剂盒
饮料、果汁、蜂蜜和食品中蔗糖和葡萄糖的分析
K-SUFRG
蔗糖/D-果糖/D-葡萄糖检测试剂盒
适用于植物和食品中蔗糖、D-葡萄糖和D-果糖的测定
K-TDFR
总膳食纤维检测试剂盒
总膳食纤维检测
K-TREH
海藻糖检测试剂盒
快速、可靠地检测食品、饮料和其它物料中海藻糖含量
K-URAMR
尿素/氨快速检测试剂盒
适用于水、饮料、乳制品和食品中尿素和氨的快速测定
K-URONIC
D-葡萄糖醛酸/D-半乳糖醛酸检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中六元糖醛酸含量（D-葡萄糖醛酸和D-半乳糖醛酸）
K-XYLOSE
D-木糖检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中D-木糖含量
K-YBGL
Beta葡聚糖[酵母和蘑菇]检测试剂盒
检测酵母和蘑菇制品中1,3:1,6-beta-葡聚糖和α-葡聚糖含量
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