>
产品中心 >
Other_kits >
Megazyme/Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method)/K-MBG4/100 assays (manual) / 400 assays (auto-analyser)
Maltβ-glucanase:
100assays(manualformat)/ 400assays(auto-analyserformat)
Lichenase:
100/200assays(manualformat)/ 330assays(auto-analyserformat)
TheMBG4reagentcontainsasinglesubstrate,namely4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(31-β-D-cellotriosyl-glucoside)(BCNPBG4).Thebenzylideneacetalgrouppreventsanyhydrolyticactionbyexo-actinghydrolyticenzymessuchasβ-glucosidaseorcellobiohydrolase.Mixedlinkage
β-glucanase(endo-1,3:1,4-β-glucanase)/lichenase(EC3.2.1.73)actsspecificallytorelease2-chloro-4-nitrophenol(CNP)fromthissubstrate.TherateofreleaseofCNPisdirectlyrelatedtotheβ-glucanase/lichenaseactivityinasample.ThereactionisterminatedandthephenolatecolourisdevelopedonadditionofTrisbuffersolution(pH=10.0).
Notethatthesubstrateisnothydrolysedbyβ-glucosidaseorcellobiohydrolase.Thesubstratecanbehydrolysedbycertainendo-cellulases(e.g.Trichodermasp.)butthisdoesnotresultinanincreaseinabsorbance.
Datacalculatorsarelocatedinthe Documentationtab.
Novelapproachestotheautomatedassayofβ-glucanaseandlichenaseactivity.
Colourimetricmethodforthedeterminationof endo-1,3:1.4-β-glucanase/lichenaseincrudemaltextractsand enzymepreparations
Principle:
(endo-1,3:1,4-β-glucanase/lichenase)
(1) Benzylidene-BGTETB-β-CNP+H2O→Benzymidene-BGTETB +CNP
(trisbuffer)
(2)CNP →phenolateion(yellowcolour)
Note: CNP=2-Chloro-4-nitrophenol
Kitsize:
Maltβ-glucanase100assays(manual)/400(auto-analyser)
or
Lichenase100/200assays(manual)/330(auto-analyser)Method: Spectrophotometricat400nm
Totalassaytime:
Maltβ-glucanase20min(manual)/10min(auto-analyser)
or
Lichenase10min(manual)/10min(auto-analyser)
Detectionlimit:
Maltβ-glucanase4.3x10-4U/mL
or
Lichenase9.1x10-5U/mL
Applicationexamples:
Crudemaltextracts,industrialenzymepreparations
Methodrecognition: Novelmethod
Advantages
- Verycosteffective
- Allreagentsstablefor>2years
- Specificfor endo-1,3:1,4-β-glucanase/lichenase
- Simple, convenient,rapidassay
- Wellsuitedtoautomation
- Maltflourstandardandlichenasestandardincluded