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Megazyme/Glucose Oxidase Assay Kit/K-GLOX/200 assays (manual) / 2000 assays (microplate) / 1960 assays (auto-analyser)
Megazyme/Glucose Oxidase Assay Kit/K-GLOX/200 assays (manual) / 2000 assays (microplate) / 1960 assays (auto-analyser)
商品编号：
K-GLOX
品牌：
Megazyme INC
市场价：
¥3360.00
美元价：
2016.00
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
TheGlucoseOxidaseassaykitisa simpleprocedurefortherapidandreliablemeasurementandanalysisofglucoseoxidaseactivityinindustrialenzymepreparationsandbreadimprovermixtures.
GlucoseOxidaseassaykitissuitableformanual,auto-analyserandmicroplateformats.
Starchdigestibilityandpredictedglycemicindexoffriedsweetpotatocultivars.
Odenigbo,A.,Rahimi,J.,NgADI,M.,Amer,S.&Mustafa,A.(2012).FunctionalFoodsinHealthandDisease,2(7),280-289.
LinktoArticle
ReadAbstract
Background:Sweetpotato(IpomoeabatatasL.)isaveryrichsourceofstarch.Thereisincreasedinterestinstarchdigestibilityandthepreventionandmanagementofmetabolicdiseases.Objective:Theaimofthisstudywastoevaluatethelevelsofstarchfractionsandpredictedglycemicindexofdifferentcultivarsofsweetpotato.MaterialandMethod:Frenchfriesproducedfromfivecultivarsofsweetpotato(‘GinsengRed’,‘Beauregard’,‘WhiteTravis’,‘GeorgiaJetclone#2010’and‘GeorgiaJet’)wereused.Theleveloftotalstarch(TS),resistantstarch(RS),digestIBLestarch(DS),andstarchdigestionindexstarchdigestionindexinthesampleswereevaluated.Invitrostarchhydrolysisat30,90,and120minweredeterminedenzymaticallyforcalculationofrapidlydigestiblestarch(RDS),predictedglycemicindex(pGI)andslowlydigestiblestarch(SDS)respectively.Results:TheRScontentinallsampleshadaninverselysignificantcorrelationwithpGI(-0.52;P<0.05) while="" rds="" had="" positive="" and="" significant="" influence="" on="" both="" pgi="">r=0.55;P<0.05) and="" sdi="">r=0.94;P<0.01). ‘white="" travis’="" and="" ‘ginseng="" red’="" had="" higher="" levels="" of="" beneficial="" starch="" fractions="" (rs="" and="" sds)="" with="" low="" pgi="" and="" starch="" digestion="" index="" (sdi),="" despite="" their="" higher="" ts="" content.="" generally,="" all="" the="" cultivars="" had="" products="" with="" low="" to="" moderate="" gi="" values.="">Conclusion:Theglycemicindexofthesefoodproductshighlightsthehealthpromotingcharacteristicsofsweetpotatocultivars.
Synthesisandcharacterizationofenzyme–magneticnanoparticlecomplexes:effectofsizeonactivityandrecovery.
Park,H.J.,McConnell,J.T.,Boddohi,S.,Kipper,M.J.&Johnson,P.A.(2011).ColloidsandSurfacesB:Biointerfaces,83(2),198-203.
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TheinfluenceofparticlesizeontheactivityandrecyclingcapABIlitiesofenzymeconjugatedmagneticnanoparticleswasstudied.Co-precipitationandoxidationofFe(OH)2methodswereusedtofabricatethreedifferentsizesofmagneticnanoparticles(5nm,26nmand51nm).Glucoseoxidasewascovalentlyboundtothemagneticnanoparticlesbymodifyingthesurfaceswith3-(aminopropyl)triethoxysilane(APTES)andacommonproteincrosslinkingagent,glutaraldehyde.AnalysisbyTransmissionElectronMicroscopy(TEM)showedthatthemorphologyofthemagneticnanoparticlestobesphericalandsizesagreedwithresultsoftheBrunauer,Emmett,andTeller(BET)method.Magneticstrengthofthenanoparticleswasanalyzedbymagnetometryandfoundtobe49emug-1(5nm),73emug-1(26nm),and85emug-1(51nm).X-rayphotoelectronspectroscopy(XPS)confirmedeachstepofthemagneticnanoparticlesurfacemodificationandsuccessfulglucoseoxidasebinding.Theimmobilizedenzymesretained15–23%ofthenativeGOxactivity.Recyclingstabilitystudiesshowedapproximately20%ofactivitylossforthelarge(51nm)andmedium(26nm)sizeglucoseoxidase-magneticnanoparticle(GOx-MNP)bioconjugateandabout96%activitylossforthesmallestGOx-MNPbioconjugate(5nm)aftertencycles.Thebioconjugatesdemonstratedequivalenttotalproductconversionsasasinglereactionofanequivalentamountofthenativeenzymeafterthe5thcycleforthe26nmnanoparticlesandthe7thcycleforthe51nmnanoparticles.
Developmentofantimicrobialpackagingmaterialswithimmobilizedglucoseoxidaseandlysozyme.
Hanušová,K.,Vápenka,L.,Dobiáš,J.&Mišková,L.(2013).CentralEuropeanJournalofChemistry,11(7),1066-1078.
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Packagingbasedonimmobilizationofantimicrobialenzymesprovidesapromisingformofactivepackagingsystemsapplicableinfoodprocessing.GlucoseoxidaseandlysozymewereimmobilizedbytheUgireactionwithcyclohexylisocyanideandglutaraldehydeonpolyamideandionomerfilmspartiallyhydrolysedbyhydrochloricacid.TheimmobilizationoftheenzymesonthesurfaceoffilmswasconfirmedbyFT-IRspectroscopyandthefilmswerecharacterizedbythespecificactivityoftheimmobilizedenzymes.TheenzymemigrationintomodelsolutionsandtheeffectofpH,temperatureandstoragetimeontheactivityofimmobilizedenzymewerealsoevaluated.Immobilizationoflysozymeontopolyamideandionomerfilmsresultedinthelossofenzymeactivity.ThepolyamideandionomerfilmswithimmobilizedglucoseoxidaseinhibitedthegrowthofbacteriaEscherichiacoliCNCTC6859,PseudomonasfluorescensCNCTC5793,LactobacillushelveticusCH-1,ListeriaivanoviiCCM5884andListeriainnocuaCCM4030onagarmedia.
Quantificationofstarchinplanttissues.
Smith,A.M.&Zeeman,S.C.(2006).NatureProtocols,1(3),1342-1345.
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Thisprotocoldescribesasimplemeansofmeasuringthestarchcontentofplanttissuesbysolubilizingthestarch,convertingitquantitativelytoglucoseandassayingtheglucose.Planttissuemustinitiallybefrozenrapidlytostopmetabolism,thenextractedtoremovefreeglucose.Starchissolubilizedbyheating,thendigestedtoglucosebyaddingglucanhydrolases.Glucoseisassayedenzymatically.Themethodismoresensitiveandaccuratethaniodine-basedprotocols,andissuitablefortissuesthathaveawiderangeofstarchcontents.Measurementsonmultiplesamplescanbecompletedwithinaday.
Freenonimmobilizedligandsasatoolforpurificationofproteins.
Patchornik,G.&Albeck,A.(2005).BioconjugateChemistry,16(5),1310-1315.
LinktoArticle
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Purificationofproteinsonalargescaleisacomplexmultistepprocess,andalternativeeconomicstrategiesarerequired.Thisstudypresentsanovelapproach(AffinitySinking,AS)forpurificationofnativeproteinsutilizingnonimmobilizedmodifiedligands.Thenonimmobilizedstateoftheligandcircumventstheneedforimmobilizingligandstopolymericsupports.Therefore,purificationfromlargevolumescanbeaccomplishedwithouttheuseofindustrial-scaleaffinitycolumns.Themechanismofproductcaptureisformationandprecipitationofaspecific[target-protein/modified-ligand]complexbyusingasolubleinterconnectingentitythatgeneratesaninsoluble[target-protein/modified-ligand/interconnectingentity]sedimentcontainingthetargetprotein.RabbitIgGandtwoglycoproteinswerepurifiedaccordingly,utilizingfreeavidin(astheinterconnectingentity)andeitherdesthiobiotinylated-proteinA(DB-ProA)ordesthiobiotinylated-concanavalinA(DB-ConA)asthemodifiedligand.TherecoveryyieldsfortheIgGandthetwoglycoproteinswere80−86%and70−75%,respectively.Targetproteinsareelutedfromthegeneratedpelletnearlywithoutdisruptingthe[modified-ligand/interconnectingentity]macro-complex,thusenablingapracticalprocedureofrecoveringtargetproteins.LeachingoftheDB-ProAligandunderelutingconditions(pH3)wasfoundtobelowerthan1%.Thetwomodifiedligands,DB-ProAandDB-ConA,wereregeneratedwithoutanychromatographicprocedurein80%and85%-89%yield,respectively.Theadvantageofexcludingthepolymericcomponentfromthepurificationprocessandobtaininghighlypurifiedproteinshasbeendemonstrated,anditimpliesthatothercontaminants(e.g.endotoxins,prions,hostDNA)couldbeexcludedaswell,therebyreducingthenumberofpurificationstepsinatypicaldownstreamprocess.
Ontherelationshipbetweenjettedinksandprintedbiopatterns:Molecular-thinfunctionalmicroarraysofglucoseoxidase.
Arrabito,G.,Musumeci,C.,Aiello,V.,Libertino,S.,Compagnini,G.&Pignataro,B.(2009).Langmuir,25(11),6312-6318.
LinktoArticle
ReadAbstract
Arraysofcircularspotsofglucoseoxidasehavebeenobtainedonfunctionalizedsiliconoxidebypiezoelectricinkjetprintingandtheenzymaticactivitytowardglucoserecognitionhasbeenmonitored.Theadditionofglyceroltothemolecularinkallowstoobtainhighspotdefinitionandresolution(tensofmicrometerswide;onemoleculetall),butinspiteofitswell-knownstructuralstabilizingproperties,indynamicconditionsitmayleadtoincreasedproteinstresses.Thejettingvoltageandpulselengthhavebeenfoundtobecriticalfactorsforbothactivityretentionandpatterndefinition.Highvoltagesandpulselengthsresultsinstresseffectsalongwiththelossofactivity,which,atleastinourexperimentalconditions,hasbeenfoundtoberecoveredintime.
Starchfractionprofilesofmilled,nonparboiledricevarietiesfromNigeria.
Odenigbo,A.M.,Ngadi,M.,Manful,J.&Danbaba,N.(2013).InternationalJournalofFoodScience&Technology,48(12),2535-2540.
LinktoArticle
ReadAbstract
Thisstudydeterminedthelevelsofnutritionallyimportantstarchfractionsinselectedmilled,nonparboiledricecultivatedinNigeria.Fiveimprovedvarieties(FARO52,FARO57,FARO44,FARO60andFARO61)andfourlocalvarieties(Kwandala,Yardass,JeepandJamila)wereevaluated.Thereweresignificantdifferencesinstarchfractionsamongvarieties.Resistantstarch(RS)rangedbetween1.43%and3.13%.Rapidlydigestiblestarch(RDS)waslowestinJamila(27.70%)andhighestinFARO61(39.26%).Generally,thelocalvarietieshadsignificanthigherRS(2.71%)withalowerRDS(32.82%)comparedwithimprovedvarieties(RS;1.88%andRDS;36.07%).RSwasinverselyrelatedtoRDSandstarchdigestionindex(SDI).TheSDIhadahighlysignificantpositivecorrelationwithRDS(r=0.879,P<0.01).=""these=""results=""highlight=""the=""need=""for=""further=""work=""in=""the=""identification=""of=""milled,=""nonparboiled=""rice=""varieties=""with=""less=""rapid=""digestion=""for=""its=""associated=""health=""benefits=""to=""consumers.="">
ColourimetricmethodforthedeterminationofGlucoseOxidase
infoodstuffsandfermentationproducts
Principle:
(glucoseoxidase)
(1)β-D-Glucose+H2O+O2→D-glucono-δ-lactone+H2O2
(peroxidase)
(2)2H2O2+p-hydroxybenzoicacid+4-aminoantipyrine→
quinoneimine+4H2O
Kitsize: 200assays(manual)/2000(microplate)
/1960(auto-analyser)
Method: Spectrophotometricat510nm
Reactiontime: ~20min
Detectionlimit: 10U/L
Applicationexamples:
Enzymepreparations,andothermaterials(e.g.BIOLOGicalcultures,
samples,etc.)
Methodrecognition: Novelmethod
Advantages
- Verycompetitiveprice(costpertest)
- Allreagentsstablefor>12monthsafterpreparation
- Simpleformat
- Mega-Calc™softwaretoolisavailablefromourwebsiteforhassle-freerawdataprocessing
- Standardincluded
- Suitableformanual,microplateandauto-analyserformats
品牌介绍
Megazyme品牌产品简介
来源：作者：人气：2149发表时间：2016-05-19 10:59:00【大 中 小】
Megazyme是一家全球性公司，专注于开发和提供用于饮料、谷物、乳制品、食品、饲料、发酵、生物燃料和葡萄酒产业用的分析试剂、酶和检测试剂盒。Megazyme的许多检测试剂盒产品已经为众多官方科学协会（包括AOAC, AACC , RACI, EBC和ICC等），经过严格的审核，批准认证为官方标准方法，确保以准确、可靠、定量和易于使用的测试方法，满足客户的质量诉求。
Megazyme的主要产品线包括：
◆ 检测试剂盒
◆ 酶
◆ 酶底物
◆ 碳水化合物
◆ 化学品/仪器
官网地址：http://www.megazyme.com
检测试剂盒特色产品：
货号
中文品名
用途
K-ACETAF
乙酸[AF法]检测试剂盒
酶法定量分析乙酸最广泛使用的方法
K-ACHDF
可吸收糖/膳食纤维检测试剂盒
酒精沉淀法测定膳食纤维
K-AMIAR
氨快速检测试剂盒
用于包括葡萄汁、葡萄酒以及其它食品饮料样品中氨含量的快速检测分析。
K-AMYL
直链淀粉/支链淀粉检测试剂盒
谷物淀粉和而粉中直链淀粉/支链淀粉比例和含量检测
K-ARAB
阿拉伯聚糖检测试剂盒
果汁浓缩液中阿拉伯聚糖的检测
K-ASNAM
L-天冬酰胺/L-谷氨酰胺和氨快速检测试剂盒
用于食品工业中丙烯酰胺前体、细胞培养基、以及上清液组分中、L-天冬酰胺，谷氨酰胺和氨的检测分析
K-ASPTM
阿斯巴甜检测试剂盒
专业用于测定饮料和食品中阿斯巴甜含量，操作简单
K-BETA3
β-淀粉酶检测试剂盒
适用于麦芽粉中β-淀粉酶的测定
K-BGLU
混合键β-葡聚糖检测试剂盒
测定谷物、荞麦粉、麦汁、啤酒及其它食品中混合键β-葡聚糖(1,3:1,4-β-D-葡聚糖)的含量
K-CERA
α-淀粉酶检测试剂盒
谷物和发酵液（真菌和细菌）中α-淀粉酶的分析测定
K-CITR
柠檬酸检测试剂盒
快速、可靠地检测食品、饮料和其它物料中柠檬酸（柠檬酸盐）含量
K-DLATE
乳酸快速检测试剂盒
快速、特异性检测饮料、肉类、奶制品和其它食品中L-乳酸和D-乳酸(乳酸盐）含量
K-EBHLG
酵母β-葡聚糖酶检测试剂盒
用于测量和分析酵母中1,3:1,6?-β-葡聚糖，也可以检测1,3-葡聚糖
K-ETSULPH
总亚硫酸检测试剂盒
测定葡萄酒、饮料、食品和其他物料中总亚硫酸含量（按二氧化硫计）的一种简单，高效，可靠的酶法检测方法
K-FRGLMQ
D-果糖/D-葡萄糖[MegaQuant法]检测试剂盒
适用于使用megaquant?色度计（505nm下）测定葡萄、葡萄汁和葡萄酒中D-果糖和D-葡萄糖的含量。
K-FRUC
果聚糖检测试剂盒
含有淀粉、蔗糖和其他糖类的植物提取物和食品中果聚糖的含量测定。
K-FRUGL
D-果糖/D-葡萄糖检测试剂盒
对植物和食品中果糖或葡萄糖含量的酶法紫外分光测定。
K-GALM
半乳甘露聚糖检测试剂盒
食品和植物产品中半乳甘露聚糖的含量检测
K-GLUC
D-葡萄糖[GOPOD]检测试剂盒
谷物提取物中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUHK
D-葡萄糖[HK]检测试剂盒
植物和食品中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUM
葡甘聚糖检测试剂盒
植物和食品中葡甘聚糖的含量测定。
K-INTDF
总膳食纤维检测试剂盒
总膳食纤维特定检测和分析
K-LACGAR
乳糖/D-半乳糖快速检测试剂盒
用于快速检测食品和植物产品中乳糖、D-半乳糖和L-阿拉伯糖
K-LACSU
乳糖/蔗糖/D-葡萄糖检测试剂盒
混合面粉和其它物料中蔗糖、乳糖和D-葡萄糖的测定
K-LACTUL
乳果糖检测试剂盒
特异性、快速和灵敏测量奶基样品中乳果糖含量
K-MANGL
D-甘露糖/D-果糖/D-葡萄糖检测试剂盒
适合测定植物产品和多糖酸性水解产物中D-甘露糖含量
K-MASUG
麦芽糖/蔗糖/D-葡萄糖检测试剂盒
在植物和食品中麦芽糖，蔗糖和葡萄糖的含量检测
K-PECID
胶质识别检测试剂盒
食品配料中果胶的鉴别
K-PHYT
植酸(总磷)检测试剂盒
食品和饲料样品植酸/总磷含量测量的简便方法。不需要通过阴离子交换色谱对植酸纯化，适合于大量样本分析
K-PYRUV
丙酮酸检测试剂盒
在啤酒、葡萄酒、果汁、食品和体液中丙酮酸分析
K-RAFGA
棉子糖/D-半乳糖检测试剂盒
快速测量植物材料和食品中棉子糖和半乳糖含量
K-RAFGL
棉子糖/蔗糖/D-半乳糖检测试剂盒
分析种子和种子粉中D-葡萄糖、蔗糖、棉子糖、水苏糖和毛蕊花糖含量。通过将棉子糖、水苏糖和毛蕊花糖酶解D-葡萄糖、D-果糖和半乳糖，从而测定葡萄糖含量来确定
K-SDAM
淀粉损伤检测试剂盒
谷物面粉中淀粉损伤的检测和分析
K-SUCGL
蔗糖/D-葡萄糖检测试剂盒
饮料、果汁、蜂蜜和食品中蔗糖和葡萄糖的分析
K-SUFRG
蔗糖/D-果糖/D-葡萄糖检测试剂盒
适用于植物和食品中蔗糖、D-葡萄糖和D-果糖的测定
K-TDFR
总膳食纤维检测试剂盒
总膳食纤维检测
K-TREH
海藻糖检测试剂盒
快速、可靠地检测食品、饮料和其它物料中海藻糖含量
K-URAMR
尿素/氨快速检测试剂盒
适用于水、饮料、乳制品和食品中尿素和氨的快速测定
K-URONIC
D-葡萄糖醛酸/D-半乳糖醛酸检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中六元糖醛酸含量（D-葡萄糖醛酸和D-半乳糖醛酸）
K-XYLOSE
D-木糖检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中D-木糖含量
K-YBGL
Beta葡聚糖[酵母和蘑菇]检测试剂盒
检测酵母和蘑菇制品中1,3:1,6-beta-葡聚糖和α-葡聚糖含量
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