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Megazyme/α-Glucuronidase Assay Kit/K-AGLUA/50 assays (manual) / 200 assays (microplate)

Theα-D-Glucuronidasetestkitisasimple,reliableandaccuratemethodforthemeasurementandanalysisofα-D-glucuronidaseinvariousenzymepreparations.Thekitcontainsapurealdotriouronicacidsubstrateandanα-D-glucuronidase(GH67)controlenzyme.
Suitableformanualandmicroplateformats.
New,improvedsubstrate.
Thiskitcontainshighlypurified,borohydridereducedaldotriouronicacidwithaterminalα-D-glucuronicacidsubstitution,whichisanexcellentsubstrateforα-D-glucuronidasesfromGH67.Thissubstratecanbealsousedforthemeasurementofα-D-glucuronidasesfromGH115,howevertherateofhydrolysiscomparedtoGH67isreduced.Previouslythiskitcontainedamixtureofborohydridereducedaldouronicacids(tri:tetra:penta).
EvidencethatGH115α-glucuronidaseactivity,whichisrequiredtodegradeplantbiomass,isdependentonconformationalflexibility.
ExpressionandCharacterizationofGlycosylHydrolaseFamily115α-GlucuronidasefromScheffersomycesstipitis.
UV-methodforthemeasurementofα-D-Glucuronidasein
variousenzymepreparations
Principle:
(α-D-glucuronidase)
(1)Aldouronicacid(NaBH4reduced)+H2O→
β-(1,4)-D-xylo-oligosaccharides+D-glucuronicacid
(uronatedehydrogenase;UDH)
(2)D-Glucuronicacid+NAD+ +H2O→D-glucarate+NADH+H+
Kitsize: 50assays(manual)/200(microplate)
Method: Spectrophotometricat340nm
Reactiontime: ~25min
Detectionlimit: 17mU/mL
Applicationexamples:
Enzymepreparationsandothermaterials
Methodrecognition: Novelmethod
Advantages
- Verycompetitiveprice(costpertest)
- Allreagentsstablefor>2yearsassupplied
- Simpleformat
- Mega-Calc™softwaretoolisavailablefromourwebsiteforhassle-freerawdataprocessing
- Standardincluded
- Suitableformanual,microplateandauto-analyserformats