Megazyme/淀粉酶HR试剂/R-AMHR4/200分析/试剂盒/4瓶
商品编号：
R-AMHR4
品牌：
Megazyme INC
市场价：
¥7728.00
美元价：
4636.80
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
HighpurityAmylaseHRReagent–4vialsforthemeasurementofα-amylaseforresearch,biochemicalenzymeassaysandinvitrodiagnosticanalysis.
Anewamylaseassayreagentcontainingp-nitrophenylα-D-maltoheptaoside(blocked)plusaThermostableα-glucosidase.Theincorporationofthisenzymeallowsassaystobeperformedattemperaturesupto60°CandoverthepHrange5.2-7.5.
Analysisoffeedenzymes.
McCleary,B.V.(2001).“EnzymesinFarmAnimalNutrition”,(M.BedfordandG.Partridge,Eds.),CABInternational,pp.85-107.
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Enzymesareaddedtoanimalfeedtoincreaseitsdigestibility,toremoveanti-nutritionalfactors,toimprovetheavailABIlityofcomponents,andforenvironmentreasons(CampbellandBedford,1992;Walshetal.,1993).Awide-varietyofcarbohydrase,protease,phytaseandlipaseenzymesfinduseinanimalfeeds.Inmonogastricdiets,enzymeactivitymustbesufficientlyhightoallowfortherelativelyshorttransittime.Also,theenzymeemployedmustbeabletoresistunfavourableconditionsthatmaybeexperiencedinfeedpreparation(e.g.extrusionandpelleting)andthatexistinthegastrointestinaltract.Measurementoftracelevelsofenzymesinanimalfeedmixturesisdifficult.Independentoftheenzymestudied,manyoftheproblemsexperiencedaresimilar;namely,lowlevelsofactivity,extractionproblemsinactivationduringfeedpreparation,non-specificbindingtootherfeedcomponentsandinhibitionbyspecificfeed-derivedinhibitors,e.g.specificxylanaseinhibitorsinwheatflour(Debyseretal.,1999).
Measurementofcerealα-Amylase:Anewassayprocedure.
McCleary,B.V.&Sheehan,H.(1987).JournalofCerealScience,6(3),237-251.
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Anewprocedurefortheassayofcerealα-amylasehasbeendeveloped.Thesubstrateisadefinedmaltosaccharidewithanα-linkednitrophenylgroupatthereducingendofthechain,andachemicalblockinggroupatthenon-reducingend.Thesubstrateiscompletelyresistanttoattackbyβ-amylase,glucoamylaseandα-glucosidaseandthusformsthebasisofahighlyspecificassayforα-amylase.Thereactionmixtureiscomposedofthesubstrateplusexcessquantitiesofα-glucosidaseandglucoamylase.Nitrophenyl-maltosaccharidesreleasedonactionofα-amylaseareinstantaneouslycleavedtoglucoseplusfreep-nitrophenolbytheglucoamylaseandα-glucosidase,suchthattherateofreleaseofp-nitrophenoldirectlycorrelateswithα-amylaseactivity.TheassayprocedureshowsanexcellentcorrelationwiththeFarrand,theFallingNumberandthePhadebasα-amylaseassayprocedures.
Anewprocedureforthemeasurementoffungalandbacterialα-amylase.
Sheehan,H.&McCleary,B.V.(1988).BiotechnologyTechnniques,2,289-292.
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Aprocedureforthemeasurementoffungalandbacterialα-amylaseincrudeculturefiltratesandcommercialenzymepreparationsisdescribed.Theprocedureemploysend-blocked(non-reducingend)p-nitrophenylmaltoheptaosideinthepresenceofamyloglucosidaseandα-glucosidase,andisabsolutelyspecificforα-amylase.Theassayprocedureissimple,reliableandaccurate.
Measurementofα-AmylaseinCereal,FoodandFermentationProducts.
McCleary,B.V.&Sturgeon,R.(2002).CerealFoodsWorld,47,299-310.
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InGeneral,thedevelopmentofmethodsformeasuringα-amylaseispioneeredintheclinicalchemistryfieldandthentranslatedtootherindustries,suchasthecerealsandfermentationindustries.Inmanyinstances,thistransferoftechnologyhasbeendifficultorimpossIBLetoachieveduetothepresenceofinterferingenzymesorsugarsandtodifferencesinthepropertiesoftheenzymesbeinganalysed.Thisarticledescribesmanyofthecommonlyusedmethodsformeasuringα-amylaseinthecereals,food,andfermentationindustriesanddiscussessomeoftheadvantagesandlimitationsofeach.
Measurementofα-amylaseactivityinwhitewheatflour,milledmalt,andmicrobialenzymepreparations,usingtheceralphaassay:Collaborativestudy.
McCleary,B.V.,McNally,M.,Monaghan,D.&Mugford,D.C.(2002).JournalofAOACInternational,85(5),1096-1102.
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Thisstudywasconductedtoevaluatethemethodperformanceofarapidprocedureforthemeasurementofα-amylaseactivityinfloursandmicrobialenzymepreparations.Samplesweremilled(ifnecessary)topassa0.5mmsieveandthenextractedwithabuffer/saltsolution,andtheextractswereclarifiedanddiluted.Aliquotsofdilutedextract(containingα-amylase)wereincubatedwithsubstratemixtureunderdefinedconditionsofpH,temperature,andtime.Thesubstrateusedwasnonreducingend-blockedp-nitrophenylmaltoheptaoside(BPNPG7)inthepresenceofexcessquantitiesofthermostableα-glucosidase.TheblockinggroupinBPNPG7preventshydrolysisofthissubstratebyexo-actingenzymessuchasamyloglucosidase,α-glucosidase,andβ-amylase.Whenthesubstrateiscleavedbyendo-actingα-amylase,thenitrophenyloligosaccharideisimmediatelyandcompletelyhydrolyzedtop-nitrophenolandfreeglucosebytheexcessquantitiesofα-glucosidasepresentinthesubstratemixture.Thereactionisterminated,andthephenolatecolordevelopedbytheadditionofanalkalinesolutionismeasuredat400nm.AmylaseactivityisexpressedintermsofCeralphaunits;1unitisdefinedastheamountofenzymerequiredtorelease1µmolp-nitrophenyl(inthepresenceofexcessquantitiesofα-glucosidase)in1minat40°C.Inthepresentstudy,15laboratoriesanalyzed16samplesasblindduplicates.Theanalyzedsampleswerewhitewheatflour,whitewheatflourtowhichfungalα-amylasehadbeenadded,milledmalt,andfungalandbacterialenzymepreparations.Repeatabilityrelativestandarddeviationsrangedfrom1.4to14.4%,andreproducibilityrelativestandarddeviationsrangedfrom5.0to16.7%.
Thickenersfordysphagicpatients:comparisonofanewamylaseresistantproductwithfourstandardstarch-basedproducts-invitrostudy.
Oudhuis,A.A.C.M.,Helmens,H.J.&Bos,M.A.ESPEN2:e83.2007.
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Patientswithdysphagiaarecommonlyprescribedthickeneddrinkstopromotesafeswallowing.Standardstarch-basedthickenersaresensitivetoα-amylase,andmaythinduringconsumptionresultinginpatientsnotreceivingtheirprescribedconsistency.Thisstudycomparestheeffectofhumansalivaontheconsistencyofdrinks(water,fullfatmilkandblackcoffee)thickenedwithanewlydevelopedthickenerwithα-amylaseresistantfeaturesandfourstandardstarch-basedthickeners.
Pharmacometricsof3-Methoxypterostilbene:AComponentofTrADItionalChineseMedicinalPlants.
Martinez,S.E.,Sayre,C.L.&Davies,N.M.(2013).Evidence-BasedComplementaryandAlternativeMedicine,ArticleID261468.
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3-Methoxypterostilbeneisanaturallyoccurringstilbenewithpotentialinthetreatmentofdiabetes.Thepreclinicalpharmacokineticsandpharmacodynamicsof3-methoxypterostilbenewereevaluatedinthepresentstudy.TherightjugularveinsofmaleSprague-Dawleyratswerecannulated.Theratsweredosed10 mg/kgor100 mg/kgof3-methoxypterostilbeneintravenously(IV)ororally(PO),respectively.Serumandurinesampleswereanalyzedusingapreviouslyvalidatedreversed-phaseHPLCmethod.SerumAUC,serumt1/2,urinet1/2,Cltotal,andVdforIVdosingwere48.1±23.8 µg/h/mL, 18.1±10.9h,9.54±1.51 h,47.8±23.7 L/h/kg,and5.11±0.38 L/kg,respectively(mean±SEM,n=4).SerumAUC,serumt1/2,urinet1/2,Cltotal,andVdforPOdosingwere229.8±44.6 µg/h/mL,73.3±8.91 h,20.6±3.01 h,0.48±0.008 L/h/kg,and52.0±10.5 L/kg,respectively(mean±SEM,n=4).Bioavailabilityofthestilbenewasdeterminedtobe50.6% ±10.0%.A3-methoxypterostilbeneglucuronidatedmetabolitewasdetectedinbothserumandurine.3-Methoxypterostilbeneexhibitedantidiabeticactivityincludingα-glucosidaseandα-amylaseinhibitionaswellasconcentration-dependentantioxidantcapacitysimilartoresveratrol.3-Methoxypterostilbenealsoexhibitedanti-inflammatoryactivity.3-Methoxypterostilbeneappearstobeabioactivecompoundandmaybeusefulinreducingpostprandialhyperglycemia.
Dose-andtissue-specificinteractionofmonoterpeneswiththegibberellin-mediatedreleaseofpotatotuberbuddormancy,sproutgrowthandinductionofα-amylasesandβ-amylases.
Rentzsch,S.,Podzimska,D.,Voegele,A.,Imbeck,M.,Müller,K.,Linkies,A.&Leubner-Metzger,G.(2012).Planta,235(1),137-151.
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Gibberellins(GA)areinvolvedinbuddormancyreleaseinseveralspecies.WeshowherethatGA-treatmentreleasedbuddormancy,initiatedbudsproutingandpromotedsproutgrowthofexcisedpotatotuberbuddiscs(‘eyes’).Monoterpenesfrompeppermintoil(PMO)andS-(+)-carvone(CAR)interactwiththeGA-mediatedbuddormancyreleaseinahormesis-typeresponse:lowmonoterpeneconcentrationsenhancedormancyreleaseandtheinitiationofbudsprouting,whereashighconcentrationsinhibitit.PMOandCARdid,however,notaffectsproutgrowthrateafteritsonset.WefurthershowthatGA-induceddormancyreleaseisassociatedwithtissue-specificregulationofα-andβ-amylases.Molecularphylogeneticanalysisshowsthatpotatoα-amylasesclusterintotwodistinctgroups:α-AMY1andα-AMY2.GA-treatmentinducedtranscriptaccumulationofmembersofbothα-amylasegroups,aswellasα-andβ-amylaseenzymeactivityinsproutand‘sub-eye’tissues.Insprouts,CARinteractswiththeGA-mediatedaccumulationofα-amylasetranscriptsinanα-AMY2-specificanddose-dependentmanner.LowCARconcentrationsenhancetheaccumulationofα-AMY2-typeα-amylasetranscripts,butdonotaffecttheα-AMY1-typetranscripts.LowCARconcentrationsalsoenhancetheaccumulationofα-andβ-amylaseenzymeactivityinsprouts,butnotin‘sub-eye’tissues.Incontrast,highCARconcentrationshavenoappreciableeffectinsproutsontheenzymeactivitiesandtheα-amylasetranscriptabundancesofeithergroup.Thedose-dependenteffectsontheenzymeactivitiesandtheα-AMY2-typeα-amylasetranscriptsinsproutsarespecificforCARbutnotforPMO.Differentmonoterpenesthereforemayhavespecifictargetsfortheirinteractionwithhormonesignallingpathways.
Akineticmodeltoexplainthemaximuminα-amylaseactivitymeasurementsinthepresenceofsmallcarbohydrates.
Baks,T.,Janssen,A.E.&Boom,R.M.(2006).BiotechnologyandBioengineering,94(3),431-440.
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Theeffectofthepresenceofseveralsmallcarbohydratesonthemeasurementoftheα-amylaseactivitywasdeterminedoverabroadconcentrationrange.Atlowcarbohydrateconcentrations,adistinctmaximumintheα-amylaseactivityversusconcentrationcurveswasobservedinseveralcases.Athigherconcentrations,allcarbohydratesshowadecreasingα-amylaseactivityatincreasingcarbohydrateconcentrations.Ageneralkineticmodelhasbeendevelopedthatcanbeusedtodescribeandexplainthesephenomena.Thismodelisbasedontheformationofacarbohydrate–enzymecomplexthatremainsactive.Itisassumedthatthiscomplexisformedwhenacarbohydratebindstoα-amylasewithoutblockingthecatalyticsiteanditssurroundingsubsites.Furthermore,thekineticmodelincorporatessubstrateinhibitionandsubstratecompetition.Dependingonthecarbohydratetypeandconcentration,themeasuredα-amylaseactivitycanbe75%lowerthantheactualα-amylaseactivity.Themodelthathasbeendevelopedcanbeusedtocorrectfortheseeffectsinordertoobtaintheactualamountofactiveenzyme.
TowardaMicrofluidic‐BasedRapidAmylaseAssaySystem.
Holmes,R.J.,Summersgil,P.,Ryan,T.,Brown,B.J.T.,Mockbil,A.,Grieve,B.D.&Fielden,P.R.(2009).JournalofFoodScience,74(6),N37-N43.
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Thisarticledescribesworkintoaprototypesystemfortheassayofamylase,usingmicrofludictechnologies.Thenewsystemhasasignificantlyshortercycletimethanthecurrentlaboratorymethods,whichgenerallyusemicrotitreplates,yetiscapableofgeneratingsignificantlysuperiorresults.Assuch,wehaveshownthatsensitivityisenhancedbyafactorof10inthestandardassaytrials,andbyafactorof2inthereal-samplelabtrials.Inbothassays,theuseofamicroreactorsystemreducedthereactiontimebyafactorof6.2,from20minincubationto3.2min.BasingtheconclusionontheMegazymeCerealphaStandardMethod,andusingtheCerealphaunitsasameasureofassayefficiency,thetypicalresponseforthemicrofluidicassaywasshowntobe1.0×10-3CU/mL(standarddeviation[SD]2.5×10-4CU/mL),comparedto2.56×10-4CU/mL(SD5.94×10-5CU/mL)forthestandardmacroassay.Itisbelievedthatthisimprovementinthereactionschematicsisduetotheinherentadvantagesofmicrofluidicdevicessuchassuperiormixing,higherthermalefficiency,andenhancedreactionkinetics.
EffectofGelatinizationandHydrolysisConditionsontheSelectivityofStarchHydrolysiswithα-AmylasefromBacilluslicheniformis.
Baks,T.,Bruins,M.E.,Matser,A.M.,Janssen,A.E.M.&Boom,R.M.(2008).JournalofAgriculturalandFoodChemistry,56(2),488-495.
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Enzymatichydrolysisofstarchcanbeusedtoobtainvariousvaluablehydrolyzateswithdifferentcompositions.Theeffectsofstarchpretreatment,enzymeadditionpoint,andhydrolysisconditionsonthehydrolyzatecompositionandreactionrateduringwheatstarchhydrolysiswithα-amylasefromBacilluslicheniformiswerecompared.SUSPensionsofnativestarchorstarchgelatinizedatdifferentconditionseitherwithorwithoutenzymewerehydrolyzed.Duringhydrolysis,theoligosaccharideconcentration,thedextroseequivalent,andtheenzymeactivityweredetermined.Wefoundthatthehydrolyzatecompositionwasaffectedbythetypeofstarchpretreatmentandtheenzymeadditionpointbutthatitwasjustminimallyaffectedbythepressureappliedduringhydrolysis,aslongasgelatinizationwascomplete.Thedifferencesbetweenhydrolysisofthermallygelatinized,high-pressuregelatinized,andnativestarchwereexplainedbyconsideringthegranulestructureandthespecificsurfaceareaofthegranules.Theseresultsshowthatthehydrolyzatecompositioncanbeinfluencedbychoosingdifferentprocesssequencesandconditions.
品牌介绍
Megazyme品牌产品简介
来源：作者：人气：2149发表时间：2016-05-19 10:59:00【大 中 小】
Megazyme是一家全球性公司，专注于开发和提供用于饮料、谷物、乳制品、食品、饲料、发酵、生物燃料和葡萄酒产业用的分析试剂、酶和检测试剂盒。Megazyme的许多检测试剂盒产品已经为众多官方科学协会（包括AOAC, AACC , RACI, EBC和ICC等），经过严格的审核，批准认证为官方标准方法，确保以准确、可靠、定量和易于使用的测试方法，满足客户的质量诉求。
Megazyme的主要产品线包括：
◆ 检测试剂盒
◆ 酶
◆ 酶底物
◆ 碳水化合物
◆ 化学品/仪器
官网地址：http://www.megazyme.com
检测试剂盒特色产品：
货号
中文品名
用途
K-ACETAF
乙酸[AF法]检测试剂盒
酶法定量分析乙酸最广泛使用的方法
K-ACHDF
可吸收糖/膳食纤维检测试剂盒
酒精沉淀法测定膳食纤维
K-AMIAR
氨快速检测试剂盒
用于包括葡萄汁、葡萄酒以及其它食品饮料样品中氨含量的快速检测分析。
K-AMYL
直链淀粉/支链淀粉检测试剂盒
谷物淀粉和而粉中直链淀粉/支链淀粉比例和含量检测
K-ARAB
阿拉伯聚糖检测试剂盒
果汁浓缩液中阿拉伯聚糖的检测
K-ASNAM
L-天冬酰胺/L-谷氨酰胺和氨快速检测试剂盒
用于食品工业中丙烯酰胺前体、细胞培养基、以及上清液组分中、L-天冬酰胺，谷氨酰胺和氨的检测分析
K-ASPTM
阿斯巴甜检测试剂盒
专业用于测定饮料和食品中阿斯巴甜含量，操作简单
K-BETA3
β-淀粉酶检测试剂盒
适用于麦芽粉中β-淀粉酶的测定
K-BGLU
混合键β-葡聚糖检测试剂盒
测定谷物、荞麦粉、麦汁、啤酒及其它食品中混合键β-葡聚糖(1,3:1,4-β-D-葡聚糖)的含量
K-CERA
α-淀粉酶检测试剂盒
谷物和发酵液（真菌和细菌）中α-淀粉酶的分析测定
K-CITR
柠檬酸检测试剂盒
快速、可靠地检测食品、饮料和其它物料中柠檬酸（柠檬酸盐）含量
K-DLATE
乳酸快速检测试剂盒
快速、特异性检测饮料、肉类、奶制品和其它食品中L-乳酸和D-乳酸(乳酸盐）含量
K-EBHLG
酵母β-葡聚糖酶检测试剂盒
用于测量和分析酵母中1,3:1,6?-β-葡聚糖，也可以检测1,3-葡聚糖
K-ETSULPH
总亚硫酸检测试剂盒
测定葡萄酒、饮料、食品和其他物料中总亚硫酸含量（按二氧化硫计）的一种简单，高效，可靠的酶法检测方法
K-FRGLMQ
D-果糖/D-葡萄糖[MegaQuant法]检测试剂盒
适用于使用megaquant?色度计（505nm下）测定葡萄、葡萄汁和葡萄酒中D-果糖和D-葡萄糖的含量。
K-FRUC
果聚糖检测试剂盒
含有淀粉、蔗糖和其他糖类的植物提取物和食品中果聚糖的含量测定。
K-FRUGL
D-果糖/D-葡萄糖检测试剂盒
对植物和食品中果糖或葡萄糖含量的酶法紫外分光测定。
K-GALM
半乳甘露聚糖检测试剂盒
食品和植物产品中半乳甘露聚糖的含量检测
K-GLUC
D-葡萄糖[GOPOD]检测试剂盒
谷物提取物中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUHK
D-葡萄糖[HK]检测试剂盒
植物和食品中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUM
葡甘聚糖检测试剂盒
植物和食品中葡甘聚糖的含量测定。
K-INTDF
总膳食纤维检测试剂盒
总膳食纤维特定检测和分析
K-LACGAR
乳糖/D-半乳糖快速检测试剂盒
用于快速检测食品和植物产品中乳糖、D-半乳糖和L-阿拉伯糖
K-LACSU
乳糖/蔗糖/D-葡萄糖检测试剂盒
混合面粉和其它物料中蔗糖、乳糖和D-葡萄糖的测定
K-LACTUL
乳果糖检测试剂盒
特异性、快速和灵敏测量奶基样品中乳果糖含量
K-MANGL
D-甘露糖/D-果糖/D-葡萄糖检测试剂盒
适合测定植物产品和多糖酸性水解产物中D-甘露糖含量
K-MASUG
麦芽糖/蔗糖/D-葡萄糖检测试剂盒
在植物和食品中麦芽糖，蔗糖和葡萄糖的含量检测
K-PECID
胶质识别检测试剂盒
食品配料中果胶的鉴别
K-PHYT
植酸(总磷)检测试剂盒
食品和饲料样品植酸/总磷含量测量的简便方法。不需要通过阴离子交换色谱对植酸纯化，适合于大量样本分析
K-PYRUV
丙酮酸检测试剂盒
在啤酒、葡萄酒、果汁、食品和体液中丙酮酸分析
K-RAFGA
棉子糖/D-半乳糖检测试剂盒
快速测量植物材料和食品中棉子糖和半乳糖含量
K-RAFGL
棉子糖/蔗糖/D-半乳糖检测试剂盒
分析种子和种子粉中D-葡萄糖、蔗糖、棉子糖、水苏糖和毛蕊花糖含量。通过将棉子糖、水苏糖和毛蕊花糖酶解D-葡萄糖、D-果糖和半乳糖，从而测定葡萄糖含量来确定
K-SDAM
淀粉损伤检测试剂盒
谷物面粉中淀粉损伤的检测和分析
K-SUCGL
蔗糖/D-葡萄糖检测试剂盒
饮料、果汁、蜂蜜和食品中蔗糖和葡萄糖的分析
K-SUFRG
蔗糖/D-果糖/D-葡萄糖检测试剂盒
适用于植物和食品中蔗糖、D-葡萄糖和D-果糖的测定
K-TDFR
总膳食纤维检测试剂盒
总膳食纤维检测
K-TREH
海藻糖检测试剂盒
快速、可靠地检测食品、饮料和其它物料中海藻糖含量
K-URAMR
尿素/氨快速检测试剂盒
适用于水、饮料、乳制品和食品中尿素和氨的快速测定
K-URONIC
D-葡萄糖醛酸/D-半乳糖醛酸检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中六元糖醛酸含量（D-葡萄糖醛酸和D-半乳糖醛酸）
K-XYLOSE
D-木糖检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中D-木糖含量
K-YBGL
Beta葡聚糖[酵母和蘑菇]检测试剂盒
检测酵母和蘑菇制品中1,3:1,6-beta-葡聚糖和α-葡聚糖含量
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