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Megazyme/Amyloglucosidase Assay Reagent /R-AMGR3/200 assays per kit / 4 vials
Megazyme/Amyloglucosidase Assay Reagent /R-AMGR3/200 assays per kit / 4 vials
商品编号:
R-AMGR3
品牌:
Megazyme INC
市场价:
¥6744.00
美元价:
4046.40
产品分类:
其它检测试剂盒
公司分类:
Other_kits
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
HighpurityAmyloglucosidaseAssayReagent–4vialsforthemeasurementofamyloglucosidase,forresearch,biochemicalenzymeassaysandinvitrodiagnosticanalysis.
p-Nitrophenylβ-D-maltosideplusexcessβ-glucosidase.
MeasurementofamyloglucosidaseusingP-nitrophenylβ-maltosideassubstrate.
McCleary,B.V.,Bouhet,F.&Driguez,H.(1991).BiotechnologyTechniques,5(4),255-258.
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Anenzyme-linkedassayforthemeasurementofamyloglucosidaseincommercialenzymemixturesandcrudeculturefiltratesisdescribed.Amethodforthesynthesisofthesubstrateemployed,p-nitrophenylβ-D-maltoside,isalsodescribed.Thesubstrateisusedinthepresenceofsaturatinglevelsofβ-glucosidase.WitharangeofAspergillussp.culturefiltrates,anexcellentcorrelationwasfoundforvaluesobtainedwiththisassayandaconventionalassayemployingmaltoseassubstratewithmeasurementofreleasedglucose.
FunctionalityofselectedstrainsofmouldsandyeastsfromVietnamesericewinestarters.
Dung,N.T.P.,Rombouts,F.M.&Nout,M.J.R.(2006).FoodMicroBIOLOGy,23(4),331-340.
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Theroleofstarch-degrADIngmycelialfungi,andthealcoholproductionandethanoltoleranceoftheyeastsisolatedfromselectedVietnamesetraditionalricewinestarterswereexamined,andoptimumconditionsfortheseessentialstepsinricewinefermentationweredetermined.OfpureisolatesfromVietnamesericewinestarters,mouldstrainsidentifiedasAmylomycesrouxii,Amylomycesaff.rouxii,RhizopusoligosporusandRhizopusoryzae,weresuperiorinstarchdegradation,glucoseproductionandamyloglucosidaseactivityduringthesaccharificationofpurpleglutinousrice.A.rouxiiwasabletoproduceupto25%w/wglucosewithanamyloglucosidaseactivityupto0.6Ug-1offermentedmouldedmass.FiveyeastisolatesidentifiedasSaccharomycescerevisiaewereselectedfortheirsuperioralcoholproductivity.Theywereabletodepletearelativelyhighinitialpercentageofglucose(20%w/v),forming8.8%w/vethanol.TheethanoltoleranceofS.cerevisiaeinchallengetestswas9–10%w/v,and13.4%w/vasmeasuredinfed-batchfermentations.Optimumconditionsforthesaccharificationwere:incubationfor2dat34°C,ofsteamedriceinoculatedwith5logcfug-1;forthealcoholicfermentation4dat28.3°C,ofsaccharifiedriceliquidinoculatedwith5.5logcfumL-1.
Biomimeticsilicaencapsulationofenzymesforreplacementofbiocidesinantifoulingcoatings.
Kristensen,J.B.,Meyer,R.L.,Poulsen,C.H.,Kragh,K.M.,Besenbacher,F.&Laursen,B.S.(2010).GreenChemistry,12(3),387-394.
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Currentantifoulingtechnologiesforshiphullsarebasedonmetalssuchascuprousoxideandco-biocideslikezincpyrithione.Duetothepersistentadverseenvironmentaleffectsofthesebiocides,enzyme-basedantifoulingpaintsareproposedasabio-based,non-accumulatingalternative.Here,ahydrogenperoxide-producingsystemcomposedofhexoseoxidase(HOX,EC1.1.3.5),glucoamylase(GA,EC3.2.1.3)andstarchistestedforthechemicalandphysicalfunctionalitiesnecessaryforsuccessfulincorporationintoamarinecoating.TheactivityandstABIlityoftheenzymesinseawaterwasevaluatedatdifferenttemperatures,andpaintcompatibilitywasassessedbymeasuringthedistributionandactivityoftheenzymesincorporatedintoprototypecoatingformulations.WeusedabiomimeticencapsulationprocedureforHOXthroughpolyethylenimine-templatedsilicaco-precipitation.Theco-precipitationandformulationofapowderformixingintoamarinepaintwasperformedinaone-stepeconomicalandgentleformulationprocess,inwhichsilicaco-precipitatedHOXwascombinedwithGAandstarchtoformtheantifoulingsystem.Silicaco-precipitationsignificantlyimprovedthestabilityandperformanceoftheantifoulingsysteminmarine-likeconditions.Forexample,encapsulationofHOXresultedin46%higheractivityatpH8,anditsstabilityinartificialseawaterincreasedfromretainingonly3.5%activityafter2weekstoretaining55%activityafter12weeks.Acoatingcomprisingthefullenzymesystemreleasedhydrogenperoxideatratesexceedingatargetof36nmolcm-2d-1for3monthsinalaboratoryassay,andhadpotentialforprolongedactionthroughincorporationinaself-polishingcoating.
Developmentofdefinedmixed-culturefungalfermentationstartergranulateforcontrolledproductionofricewine.
Dung,N.T.P.,Rombouts,F.M.&Nout,M.J.R.(2005).InnovativeFoodScience&EmergingTechnologies,6(4),429-441.
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Asafirststepinthedevelopmentofdefinedfungalstartergranulesforcontrolledwinemakingfrompurpleglutinousrice,theinteractionofmouldsandyeastsisolatedfromVietnamesericewinestartersandtheeffectofsomerepresentativeorientalherbsonthegrowthofmouldsandyeastswereexamined.AmylomycesrouxiiandSaccharomycescerevisaewereshowntobecompatIBLeinmixedcultures,andtheherbs“TieuHoi”(Fennel:FoeniculumvulgareMiller)and“DinhHuong”(Clove:SyzygiumaromaticumL.)whichareusedassupplementaryingredientsbysomelocalstarterproducers,wereobservedtostimulatethemouldandyeastgrowth.Basedontraditionalstartermanufacturingmethodsandmodifiedonthebasisofoptimizationexperiments,alaboratory-scalemanufacturingprocessfordefinedmixed-culturestartergranuleswasestablished.Inaccordancewiththenationalstandardmethod,thewineproducedwithnewexperimentalstartergranuleswasfoundtohavesuperiorflavourandoverallacceptability,comparedwithlocalcommercialricewines.Industrialrelevance.OneofthemajorproblemsfacedbycommercialbrewersofricewineinVietnam,isthevariablequalityandperformanceofthetraditionalstartertabletsthatarecommonlyused.Therelevanceofthepresentpaperisthatastable,granulatedstarterhasbeendeveloped,containingadefinedmixtureofmouldandyeastcultures.Thishasproventobeshelfstableformorethan3months,producingaverywellacceptedqualityofwine.
Comparativeassessmentofamylolyticandcellulolyticenzymeactivityofmaltspreparedfromtropicalcereals.
Dziedzoave,N.T.,Graffham,A.J.,Westby,A.&Komlaga,G.(2010).FoodControl,21(10),1349-1353.
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Theactivitylevelsofamylolyticenzymesandβ-glucanaseinmaltspreparedfromfourtropicalcerealgrainswereassessedtoestablishtherelativeusefulnessofthesemaltsforproductionofglucosesyrups.Ricemaltshowedthehighestactivityfortheamylolyticenzymes,whilstmilletandsorghummaltswererichestinβ-glucanaseactivity.Optimumamylolyticenzymedevelopmentinricemaltsoccurredbetween9–13days;and11daysforoptimumβ-glucanasedevelopmentinmilletmalt.β-Amylasewasthepredominantenzymeinallthecerealmaltsexceptmaize,forwhichthepredominantenzymewasα-amylase.Optionsforoptimisingtheproductionofenzymesfromthethreecereals,andtheirpotentialforuseintheproductionofglucosesyrupsarediscussed.
Microfluidicscreeningandwhole-genomesequencingidentifiesmutationsassociatedwithimprovedproteinsecretionbyyeast.
Huang,M.,Bai,Y.,Sjostrom,S.L.,Hallström,B.M.,Liu,Z.,Petranovic,D.,Uhlén,M.,Joensson,H.N.,Andersson-Svahn,H.&Nielsen,J.(2015).ProceedingsoftheNationalAcademyofSciences,112(34),E4689-E4696.
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Thereisanincreasingdemandforbiotech-basedproductionofrecombinantproteinsforuseaspharmaceuticalsinthefoodandfeedindustryandinindustrialapplications.YeastSaccharomycescerevisiaeisamongpreferredcellfactoriesforrecombinantproteinproduction,andthereisincreasinginterestinimprovingitsproteinsecretioncapacity.Duetothecomplexityofthesecretorymachineryineukaryoticcells,itisdifficulttoapplyrationalengineeringforconstructionofimprovedstrains.Hereweusedhigh-throughputmicrofluidicsforthescreeningofyeastlibraries,generatedbyUVmutagenesis.Severalscreeningandsortingroundsresultedintheselectionofeightyeastcloneswithsignificantlyimprovedsecretionofrecombinantα-amylase.Efficientsecretionwasgeneticallystableintheselectedclones.Weperformedwhole-genomesequencingoftheeightclonesandidentified330mutationsintotal.Geneontologyanalysisofmutatedgenesrevealedmanybiologicalprocesses,includingsomethathavenotbeenidentifiedbeforeinthecontextofproteinsecretion.Mutatedgenesidentifiedinthisstudycanbepotentiallyusedforreversemetabolicengineering,withtheobjectivetoconstructefficientcellfactoriesforproteinsecretion.Thecombineduseofmicrofluidicsscreeningandwhole-genomesequencingtomapthemutationsassociatedwiththeimprovedphenotypecaneasilybeadaptedforotherproductsandcelltypestoidentifynovelengineeringtargets,andthisapproachcouldbroadlyfacilitatedesignofnovelcellfactories.
品牌介绍
Megazyme品牌产品简介
来源:作者:人气:2149发表时间:2016-05-19 10:59:00【大 中 小】
Megazyme是一家全球性公司,专注于开发和提供用于饮料、谷物、乳制品、食品、饲料、发酵、生物燃料和葡萄酒产业用的分析试剂、酶和检测试剂盒。Megazyme的许多检测试剂盒产品已经为众多官方科学协会(包括AOAC, AACC , RACI, EBC和ICC等),经过严格的审核,批准认证为官方标准方法,确保以准确、可靠、定量和易于使用的测试方法,满足客户的质量诉求。
Megazyme的主要产品线包括:
◆ 检测试剂盒
◆ 酶
◆ 酶底物
◆ 碳水化合物
◆ 化学品/仪器
官网地址:http://www.megazyme.com
检测试剂盒特色产品:
货号
中文品名
用途
K-ACETAF
乙酸[AF法]检测试剂盒
酶法定量分析乙酸最广泛使用的方法
K-ACHDF
可吸收糖/膳食纤维检测试剂盒
酒精沉淀法测定膳食纤维
K-AMIAR
氨快速检测试剂盒
用于包括葡萄汁、葡萄酒以及其它食品饮料样品中氨含量的快速检测分析。
K-AMYL
直链淀粉/支链淀粉检测试剂盒
谷物淀粉和而粉中直链淀粉/支链淀粉比例和含量检测
K-ARAB
阿拉伯聚糖检测试剂盒
果汁浓缩液中阿拉伯聚糖的检测
K-ASNAM
L-天冬酰胺/L-谷氨酰胺和氨快速检测试剂盒
用于食品工业中丙烯酰胺前体、细胞培养基、以及上清液组分中、L-天冬酰胺,谷氨酰胺和氨的检测分析
K-ASPTM
阿斯巴甜检测试剂盒
专业用于测定饮料和食品中阿斯巴甜含量,操作简单
K-BETA3
β-淀粉酶检测试剂盒
适用于麦芽粉中β-淀粉酶的测定
K-BGLU
混合键β-葡聚糖检测试剂盒
测定谷物、荞麦粉、麦汁、啤酒及其它食品中混合键β-葡聚糖(1,3:1,4-β-D-葡聚糖)的含量
K-CERA
α-淀粉酶检测试剂盒
谷物和发酵液(真菌和细菌)中α-淀粉酶的分析测定
K-CITR
柠檬酸检测试剂盒
快速、可靠地检测食品、饮料和其它物料中柠檬酸(柠檬酸盐)含量
K-DLATE
乳酸快速检测试剂盒
快速、特异性检测饮料、肉类、奶制品和其它食品中L-乳酸和D-乳酸(乳酸盐)含量
K-EBHLG
酵母β-葡聚糖酶检测试剂盒
用于测量和分析酵母中1,3:1,6?-β-葡聚糖,也可以检测1,3-葡聚糖
K-ETSULPH
总亚硫酸检测试剂盒
测定葡萄酒、饮料、食品和其他物料中总亚硫酸含量(按二氧化硫计)的一种简单,高效,可靠的酶法检测方法
K-FRGLMQ
D-果糖/D-葡萄糖[MegaQuant法]检测试剂盒
适用于使用megaquant?色度计(505nm下)测定葡萄、葡萄汁和葡萄酒中D-果糖和D-葡萄糖的含量。
K-FRUC
果聚糖检测试剂盒
含有淀粉、蔗糖和其他糖类的植物提取物和食品中果聚糖的含量测定。
K-FRUGL
D-果糖/D-葡萄糖检测试剂盒
对植物和食品中果糖或葡萄糖含量的酶法紫外分光测定。
K-GALM
半乳甘露聚糖检测试剂盒
食品和植物产品中半乳甘露聚糖的含量检测
K-GLUC
D-葡萄糖[GOPOD]检测试剂盒
谷物提取物中D-葡萄糖的含量测定,可以和其它Megazyme检测试剂盒联合使用。
K-GLUHK
D-葡萄糖[HK]检测试剂盒
植物和食品中D-葡萄糖的含量测定,可以和其它Megazyme检测试剂盒联合使用。
K-GLUM
葡甘聚糖检测试剂盒
植物和食品中葡甘聚糖的含量测定。
K-INTDF
总膳食纤维检测试剂盒
总膳食纤维特定检测和分析
K-LACGAR
乳糖/D-半乳糖快速检测试剂盒
用于快速检测食品和植物产品中乳糖、D-半乳糖和L-阿拉伯糖
K-LACSU
乳糖/蔗糖/D-葡萄糖检测试剂盒
混合面粉和其它物料中蔗糖、乳糖和D-葡萄糖的测定
K-LACTUL
乳果糖检测试剂盒
特异性、快速和灵敏测量奶基样品中乳果糖含量
K-MANGL
D-甘露糖/D-果糖/D-葡萄糖检测试剂盒
适合测定植物产品和多糖酸性水解产物中D-甘露糖含量
K-MASUG
麦芽糖/蔗糖/D-葡萄糖检测试剂盒
在植物和食品中麦芽糖,蔗糖和葡萄糖的含量检测
K-PECID
胶质识别检测试剂盒
食品配料中果胶的鉴别
K-PHYT
植酸(总磷)检测试剂盒
食品和饲料样品植酸/总磷含量测量的简便方法。不需要通过阴离子交换色谱对植酸纯化,适合于大量样本分析
K-PYRUV
丙酮酸检测试剂盒
在啤酒、葡萄酒、果汁、食品和体液中丙酮酸分析
K-RAFGA
棉子糖/D-半乳糖检测试剂盒
快速测量植物材料和食品中棉子糖和半乳糖含量
K-RAFGL
棉子糖/蔗糖/D-半乳糖检测试剂盒
分析种子和种子粉中D-葡萄糖、蔗糖、棉子糖、水苏糖和毛蕊花糖含量。通过将棉子糖、水苏糖和毛蕊花糖酶解D-葡萄糖、D-果糖和半乳糖,从而测定葡萄糖含量来确定
K-SDAM
淀粉损伤检测试剂盒
谷物面粉中淀粉损伤的检测和分析
K-SUCGL
蔗糖/D-葡萄糖检测试剂盒
饮料、果汁、蜂蜜和食品中蔗糖和葡萄糖的分析
K-SUFRG
蔗糖/D-果糖/D-葡萄糖检测试剂盒
适用于植物和食品中蔗糖、D-葡萄糖和D-果糖的测定
K-TDFR
总膳食纤维检测试剂盒
总膳食纤维检测
K-TREH
海藻糖检测试剂盒
快速、可靠地检测食品、饮料和其它物料中海藻糖含量
K-URAMR
尿素/氨快速检测试剂盒
适用于水、饮料、乳制品和食品中尿素和氨的快速测定
K-URONIC
D-葡萄糖醛酸/D-半乳糖醛酸检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中六元糖醛酸含量(D-葡萄糖醛酸和D-半乳糖醛酸)
K-XYLOSE
D-木糖检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中D-木糖含量
K-YBGL
Beta葡聚糖[酵母和蘑菇]检测试剂盒
检测酵母和蘑菇制品中1,3:1,6-beta-葡聚糖和α-葡聚糖含量
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