Megazyme/乙醛分析试剂盒/K-ACHYD/50分析（手动）/500分析（微孔板）/500分析（自动分析仪）
商品编号：
K-ACHYD
品牌：
Megazyme INC
市场价：
¥2952.00
美元价：
1771.20
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
TheAcetaldehydetestkitisasimple,reliable andaccuratemethodforthemeasurementandanalysisofacetaldehydeinbeveragesandfoodstuffs.
ExtendedcofactorsstABIlity.Dissolvedcofactorsstablefor>1yearat4oC.
Suitableformanual,auto-analyserandmicroplateformats.
Grapeandwineanalysis:Oenologiststoexploitadvancedtestkits.
Charnock,S.C.&McCleary,B.V.(2005).RevuedesEnology,117,1-5.
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Itiswithoutdoubtthattestingplaysapivotalrolethroughoutthewholeofthevinificationprocess.ToproducethebestpossIBLequalitywineandtominimiseprocessproblemssuchas“stuck”fermentationortroublesomeinfections,itisnowrecognisedthatifpossibletestingshouldbeginpriortoharvestingofthegrapesandcontinuethroughtobottling.TrADItionalmethodsofwineanalysisareoftenexpensive,timeconsuming,requireeitherelaborateequipmentorspecialistexpertiseandfrequentlylackaccuracy.However,enzymaticbio-analysisenablestheaccuratemeasurementofthevastmajorityofanalytesofinteresttothewinemaker,usingjustonepieceofapparatus,thespectrophotometer(seepreviousissueNo.116foradetailedtechnicalreview).Grapejuiceandwineareamenabletoenzymatictestingasbeingliquidstheyarehomogenous,easytomanipulate,andcangenerallybeanalysedwithoutanysamplepreparation.
Megazyme“advanced”winetestkitsgeneralcharacteristicsandvalidation.
Charnock,S.J.,McCleary,B.V.,Daverede,C.&Gallant,P.(2006).ReveuedesOenologues,120,1-5.
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ManyoftheenzymatictestkitsareofficialmethodsofprestigiousorganisationssuchastheAssociationofOfficialAnalyticalChemicals(AOAC)andtheAmericanAssociationofCerealChemists(AACC)inresponsetotheinterestfromoenologists.Megazymedecidedtouseitslonghistoryofenzymaticbio-analysistomakeasignificantcontributiontothewineindustry,bythedevelopmentofarangeofadvancedenzymatictestkits.Thistaskhasnowbeensuccessfullycompletedthroughthestrategicandcomprehensiveprocessofidentifyinglimitationsofexistingenzymaticbio-analysistestkitswheretheyoccurred,andthenusingadvancedtechniques,suchasmolecularBIOLOGy(photo1),torapidlyovercomethem.Noveltestkitshavealsobeendevelopedforanalytesofemerginginteresttotheoenologist,suchasyeastavailablenitrogen(YAN;seepages2-3ofissue117article),orwherepreviouslyenzymesweresimplyeithernotavailable,orweretooexpensivetoemploy,suchasforD-mannitolanalysis.
Taraxeroneenhancesalcoholoxidationviaincreasesofalcoholdehyderogenase(ADH)andacetaldehydedehydrogenase(ALDH)activitiesandgeneexpressions.
Sung,C.K.,Kim,S.M.,Oh,C.J.,Yang,S.A.,Han,B.H.&Mo,E.K.(2012).FoodandChemicalToxicology,50(7),2508-2514.
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Thepresentstudy,taraxerone(D-friedoolean-14-en-3-one)wasisolatedfromSedumsarmentosumwithpurity96.383%,anditsenhancingeffectsonalcoholdehydrogenase(ADH)andacetaldehydedehydrogenase(ALDH)activitiesweredetermined:EC50valueswere512.42±3.12and500.16±3.23μMforADHandALDH,respectively.Inordertoobtainmoreinformationontaraxeronerelatedwiththealcoholmetabolism,40%ethanol(5mL/kgbodyweight)with0.5–1mMoftaraxeronewereadmiNISTeredtomice.Theplasmaalcoholandacetaldehydeconcentrationsoftaraxerone-treatedgroupsweresignificantlyloweredthanthoseofthecontrolgroup(p<0.01):=""approximately=""20–67%=""and=""7–57%=""lowered=""for=""plasma=""alcohol=""and=""acetaldehyde,=""respectively.=""compare=""to=""the=""control=""group,=""the=""adh=""and=""aldh=""expressions=""in=""the=""liver=""tissues=""were=""abruptly=""increased=""in=""the=""taraxerone-treated=""groups=""after=""ethanol=""exposure.=""in=""addition,=""taraxerone=""prevented=""catalase,=""superoxide=""dismutase,=""and=""reduced=""glutathione=""concentrations=""from=""the=""decrease=""induced=""by=""ethanol=""administration=""with=""the=""concentration=""dependent=""manner.="">
EffectsofChitooligosaccharideLactateSaltonActivityofAcetaldehydeDehydrogenase.
Cho,S.Y.,Yun,J.W.,Park,P.J.,Sohn,J.H.,Seo,D.B.,Lim,K.M.,Kim,W.G.&Lee,S.J.(2010).JournalofMedicinalFood,13(5),1061-1068.
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Chitooligosaccharides(COS),akindofoligosaccharidemadefromchitinorchitosan,havebeenusedapopularremedyforhangovers.InthisstudyweinvestigatedtheinvitroeffectofCOSlactatesaltonethanol-inducedcytotoxicityandtheinvitroeffectofshort-termCOSlactatesaltfeedingonethanol-inducedhangover.PretreatmentofHepG2cellswithCOSlactatesaltsignificantlyreducedethanol-inducedcytotoxicityandsuppressedgenerationofreactiveoxygenspecies.Inaddition,COSlactatesaltdose-dependentlyincreasedacetaldehydedehydrogenase(ALDH)activityinvitroandreversedtheALDHinhibitioninducedbydaidzin.FurThermore,oraladministrationofCOSlactatesalt(200 mg/kg)for5dayssignificantlydecreasedthebloodlevelsofalcoholandacetaldehydeinethanol-treatedmice.ItwasalsodemonstratedthathepaticmitochondrialALDHactivitywassignificantlyincreasedinCOSlactatesalt-treatedmice.Takentogether,thesefindingsindicatethatCOSlactatesaltmayhaveefficacyforthemanagementofalcoholichangovers.
Exploitationofthesemi‐homothalliclifecycleofSaccharomycescerevisiaeforthedevelopmentofbreedingstrategies.
Zara,G.,Mannazzu,I.,Sanna,M.L.,Orro,D.,Farris,G.A.&Budroni,M.(2008).FEMSYeastResearch,8(7),1147-1154.
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AstrainofSaccharomycescerevisiaehavingdesirablewinemakingpropertiesandhighsporeviabilitywasbredfromasemi-homothallicparentstrainwithsimilarwinemakingpropertiesbutthatproducedsixfoldfewerviablespores.BecausetheparentwashomozygousforHOandfortheMATaalleleatbothsilentHMRandHMLloci,itproducedtwoMATaandtwononmatingProgenyperascus.ToobtainasegregantabletomatewiththestableMATaprogeny,astrainofthenonmatingprogeny,previouslysubjectedtoHOdistruptionwithaKanMX4cassette,wasused.TheresultantMATαho::KanMX4transformantwasmatedtoaMATaHOsegregantandthediploidproducedwassporulatedtoallowtheisolationofasemi-homothallicdiploidsegregantdesignated2DthatlackedtheKanMX4-disruptedHOalleleasconfirmedbysequenceanalysis.Geneticanalysisindicatedgreaterhomozygosityin2DthanintheparentasassessedbyPCRatfiveloci.Thesugarconsumptionprofilesofboth2Dandtheparentingrapejuicefermentationswerethesame.Acetaldehydelevelsandpostfermentationbiofilmformationwerehigherin2Dthanintheparent.Because2Dhasacceptablewinemakingcharacteristicsbutproducessignificantlymoreviablesporesthantheparentstrain,itwillbeusefulinfuturebreedingefforts.
Potentialofthewastefrombeerfermentationbrothforbio-ethanolproductionwithoutanyadditionalenzyme,microbialcellsandcarbohydrates.
Ha,J.H.,Shah,N.,Ul-Islam,M.&Park,J.K.(2011).EnzymeandMicrobialTechnology,49(3),298-304.
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Thepotentialofthewastefrombeerfermentationbroth(WBFB)fortheproductionofbio-ethanolusingasimultaneoussaccharificationandfermentationprocesswithoutanyextraadditionsofsaccharificationenzymes,microbialcellsorcarbohydratewastested.ThemajormicrobialcellsinWBFBwereisolatedandidentified.ThevariationsincompositionsofWBFBwithstocktimewereinvestigated.TherewasresidualactivityofstarchhydrolyzingenzymesinWBFB.Theeffectsofreactionmodes,e.g.staticandshakingonbio-ethanolproductionwerestudied.After7daysofcultivationusingthesupernatantofWBFBat30°Ctheethanolconcentrationreached103.8g/Linshakingcultureand91.5g/Linstaticculture.Agitationexperimentsconductedatatemperature-profileprocessinwhichtemperaturewasincreasedfrom25to67°Cshortenedthesimultaneousprocesstime.TheoriginalWBFBwasmoreusefulthanthesupernatantofWBFBingettingthehigherconcentrationofethanolandreducingthefermentationtime.FromthiswholestudyitwasfoundthatWBFBisacheapandsuitablesourceforbio-ethanolproduction.
FunctionalimprovementofSaccharomycescerevisiaetoreducevolatileacidityinwine.
Luo,Z.,Walkey,C.J.,Madilao,L.L.,Measday,V.&Vuuren,H.J.(2013).FEMSYeastResearch,13(5),485-494.
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Controlofvolatileacidity(VA)isamajorissueforwinequality.Inthisstudy,weinvestigatedtheproductionofVAbyadeletionmutantofthefermentationstressresponsegeneAAF1inthebuddingyeastSaccharomycescerevisiae.FermentationswerecarriedoutincommercialChardonnaygrapemusttomimicindustrialwine-makingconditions.WedemonstratedthatawineyeaststraindeletedforAAF1reducedaceticacidlevelsinwinebyupto39.2%withoutincreasingtheacetaldehydelevels,revealingapotentialforindustrialapplication.DeletionofthecytosolicaldehydedehydrogenasegeneALD6alsoreducedaceticacidlevelsdramatically,butincreasedtheacetaldehydelevelsby41.4%,whichisnotdesiredbythewineindustry.Bycomparison,ALD4andtheAAF1paralogRSF2hadnoeffectsonaceticacidproductioninwine.DeletionofAAF1wasdetrimentaltothegrowthofald6Δandald4Δald6Δmutants,buthadnoeffectonaceticacidproduction.OverexpressionofAAF1dramaticallyincreasedaceticacidlevelsinwineinanAld6p-dependentmanner,indicatingthatAaf1pregulatesaceticacidproductionmainlyviaAld6p.OverexpressionofAAF1inanald4Δald6Δstrainproducedsignificantlymoreaceticacidinwinethantheald4Δald6Δmutant,suggestingthatAaf1pmayalsoregulateaceticacidsynthesisindependentlyofAld4pandAld6p.
AlcoholMetabolisminHumanCellsCausesDNADamageandActivatestheFanconiAnemia–BreastCancerSusceptibility(FA‐BRCA)DNADamageResponseNetwork.
Abraham,J.,Balbo,S.,Crabb,D.&Brooks,P.J.(2011).Alcoholism:ClinicalandExperimentalResearch,35(12),2113-2120.
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Background:WerecentlyreportedthatexposureofhumancellsinvitrotoacetaldehyderesultedinactivationoftheFanconianemia-breastcancerassociated(FA-BRCA)DNAdamageresponsenetwork.Methods:TodeterminewhetherintracellulargenerationofacetaldehydefromethanolmetabolismcancauseDNAdamageandactivatetheFA-BRCAnetwork,weengineeredHeLacellstometabolizealcoholbyexpressionofhumanalcoholdehydrogenase1B.Results:IncubationofHeLa-ADH1Bcellswithethanol(20mM)resultedinacetaldehydeaccumulationinthemediawhichwaspreventedbyco-incubationwith4-methylpyrazole(4-MP),aspecificinhibitorofADH.EthanoltreatmentofHeLa-ADH1Bcellsproduceda4-foldincreaseintheacetaldehyde-DNAadduct,N2-ethylidene-dGuo,andalsoresultedinactivationoftheFanconianemia-breastcancersusceptibility(FA-BRCA)DNAdamageresponsenetwork,asindicatedbyamonoubiquitinationofFANCD2,andphosphorylationofBRCA1.Ser1524wasidentifiedasonesiteofBRCA1phosphorylation.TheincreasedlevelsofDNAadducts,FANCD2monoubiquitination,andBRCA1phosphorylationwereallblockedby4-MP,indicatingthatacetaldehyde,ratherthanethanolitself,wasresponsibleforallthreeresponses.Importantly,theethanolconcentrationweusediswithintherangethatcanbeattainedinthehumanbodyduringsocialdrinking.Conclusions:OurresultsindicatethatintracellularmetabolismofethanoltoacetaldehyderesultsinDNAdamagewhichactivatestheFA-BRCADNAdamageresponsenetwork.
EffectofthermalprocessingduringyogurtproductionuponthedetectionofstaphylococcalenterotoxinB.
Principato,M.,Boyle,T.,Njoroge,J.,Jones,R.L.&O"Donnell,M.(2009).JournalofFoodProtection®,72(10),2212-2216.
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ThisresearchwasconductedtoexaminetheinherentpropertiesofyogurtcontaminatedwithstaphylococcalenterotoxinB(SEB).Twotypesofyogurtswereproducedforthisstudy.TypeIyogurtswereproducedbyaddingSEBatthestartofyogurtproduction,andtypeIIyogurtswereproducedbyaddingSEBafterthemilkbasehadbeenboiled.Biochemicalcharacteristicsinherenttoyogurt,includingpH,lacticacidandacetaldehydeconcentrations,wereanalyzedweeklyforeachbatchbeginningatatimejustafterproductionandthroughoutastorageperiodofatleast4weeks.Thepresenceoftoxinduringyogurtproductiondidnotresultinanysignificantbiochemicalorphysicalchangesinyogurt.However,wewereunabletodetectSEBtoxinintypeIyogurtusingacommerciallyavailableenzyme-linkedimmunosorbentassay(ELISA).Incontrast,SEBwaseasilydetectablebyourELISAintypeIIyogurtsamples.HigherlevelsofSEBwererecoveredfromtypeIIyogurtthathadbeenstoredfor1weekthanfromtypeIIyogurtthathadbeenstoredforanyotherlengthoftime.Theseresultsindicatethatthebiochemicalcharacteristicsofyogurtdidnotchangesignificantly(relativetocontrolyogurt)inthepresenceofeitherthermallyprocessedSEBornativeSEB.However,theabilitytodetectSEBbyELISAwasdependentonwhetherthetoxinhadbeenprocessed.
UV-methodforthedeterminationofAcetaldehydeinfoodstuffs,beveragesandothermaterials
Principle:
(aldehydedehydrogenase)
(1)Acetaldehyde+ NAD+ +H2O → aceticacid+NADH+H+
Kitsize: * 50assays(manual)/500(microplate)
/500(auto-analyser)
* Thenumberofmanualtestsperkitcanbedoubledifallvolumesarehalved.
ThiscanbereadilyaccommodatedusingtheMegaQuantTM Wave
Spectrophotometer(D-MQWAVE).
Method: Spectrophotometricat340nm
Reactiontime: ~4min
Detectionlimit: 0.18mg/L
Applicationexamples:
Wine,champagne,beer,liqueurs,brandy,dairyproducts(e.g.yogurt),
bread,fruitjuices,softdrinks,cocoa,vegetableandfruitproducts,
coffee,andothermaterials(e.g.biologicalcultures,samples,etc.)
Methodrecognition:
MethodsbasedonthisprinciplehavebeenacceptedbyMEBAK
Advantages
- Nowastedaldehydedehydrogenasesolution(stablesUSPensionsupplied)
- Verycompetitiveprice(costpertest)
- Allreagentsstablefor>2yearsafterpreparation
- Simpleformat
- Mega-Calc™softwaretoolisavailablefromourwebsiteforhassle-freerawdataprocessing
- Standardincluded
- Extendedcofactorsstability
- Suitableformanual,microplateandauto-analyserformats
品牌介绍
Megazyme品牌产品简介
来源：作者：人气：2149发表时间：2016-05-19 10:59:00【大 中 小】
Megazyme是一家全球性公司，专注于开发和提供用于饮料、谷物、乳制品、食品、饲料、发酵、生物燃料和葡萄酒产业用的分析试剂、酶和检测试剂盒。Megazyme的许多检测试剂盒产品已经为众多官方科学协会（包括AOAC, AACC , RACI, EBC和ICC等），经过严格的审核，批准认证为官方标准方法，确保以准确、可靠、定量和易于使用的测试方法，满足客户的质量诉求。
Megazyme的主要产品线包括：
◆ 检测试剂盒
◆ 酶
◆ 酶底物
◆ 碳水化合物
◆ 化学品/仪器
官网地址：http://www.megazyme.com
检测试剂盒特色产品：
货号
中文品名
用途
K-ACETAF
乙酸[AF法]检测试剂盒
酶法定量分析乙酸最广泛使用的方法
K-ACHDF
可吸收糖/膳食纤维检测试剂盒
酒精沉淀法测定膳食纤维
K-AMIAR
氨快速检测试剂盒
用于包括葡萄汁、葡萄酒以及其它食品饮料样品中氨含量的快速检测分析。
K-AMYL
直链淀粉/支链淀粉检测试剂盒
谷物淀粉和而粉中直链淀粉/支链淀粉比例和含量检测
K-ARAB
阿拉伯聚糖检测试剂盒
果汁浓缩液中阿拉伯聚糖的检测
K-ASNAM
L-天冬酰胺/L-谷氨酰胺和氨快速检测试剂盒
用于食品工业中丙烯酰胺前体、细胞培养基、以及上清液组分中、L-天冬酰胺，谷氨酰胺和氨的检测分析
K-ASPTM
阿斯巴甜检测试剂盒
专业用于测定饮料和食品中阿斯巴甜含量，操作简单
K-BETA3
β-淀粉酶检测试剂盒
适用于麦芽粉中β-淀粉酶的测定
K-BGLU
混合键β-葡聚糖检测试剂盒
测定谷物、荞麦粉、麦汁、啤酒及其它食品中混合键β-葡聚糖(1,3:1,4-β-D-葡聚糖)的含量
K-CERA
α-淀粉酶检测试剂盒
谷物和发酵液（真菌和细菌）中α-淀粉酶的分析测定
K-CITR
柠檬酸检测试剂盒
快速、可靠地检测食品、饮料和其它物料中柠檬酸（柠檬酸盐）含量
K-DLATE
乳酸快速检测试剂盒
快速、特异性检测饮料、肉类、奶制品和其它食品中L-乳酸和D-乳酸(乳酸盐）含量
K-EBHLG
酵母β-葡聚糖酶检测试剂盒
用于测量和分析酵母中1,3:1,6?-β-葡聚糖，也可以检测1,3-葡聚糖
K-ETSULPH
总亚硫酸检测试剂盒
测定葡萄酒、饮料、食品和其他物料中总亚硫酸含量（按二氧化硫计）的一种简单，高效，可靠的酶法检测方法
K-FRGLMQ
D-果糖/D-葡萄糖[MegaQuant法]检测试剂盒
适用于使用megaquant?色度计（505nm下）测定葡萄、葡萄汁和葡萄酒中D-果糖和D-葡萄糖的含量。
K-FRUC
果聚糖检测试剂盒
含有淀粉、蔗糖和其他糖类的植物提取物和食品中果聚糖的含量测定。
K-FRUGL
D-果糖/D-葡萄糖检测试剂盒
对植物和食品中果糖或葡萄糖含量的酶法紫外分光测定。
K-GALM
半乳甘露聚糖检测试剂盒
食品和植物产品中半乳甘露聚糖的含量检测
K-GLUC
D-葡萄糖[GOPOD]检测试剂盒
谷物提取物中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUHK
D-葡萄糖[HK]检测试剂盒
植物和食品中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUM
葡甘聚糖检测试剂盒
植物和食品中葡甘聚糖的含量测定。
K-INTDF
总膳食纤维检测试剂盒
总膳食纤维特定检测和分析
K-LACGAR
乳糖/D-半乳糖快速检测试剂盒
用于快速检测食品和植物产品中乳糖、D-半乳糖和L-阿拉伯糖
K-LACSU
乳糖/蔗糖/D-葡萄糖检测试剂盒
混合面粉和其它物料中蔗糖、乳糖和D-葡萄糖的测定
K-LACTUL
乳果糖检测试剂盒
特异性、快速和灵敏测量奶基样品中乳果糖含量
K-MANGL
D-甘露糖/D-果糖/D-葡萄糖检测试剂盒
适合测定植物产品和多糖酸性水解产物中D-甘露糖含量
K-MASUG
麦芽糖/蔗糖/D-葡萄糖检测试剂盒
在植物和食品中麦芽糖，蔗糖和葡萄糖的含量检测
K-PECID
胶质识别检测试剂盒
食品配料中果胶的鉴别
K-PHYT
植酸(总磷)检测试剂盒
食品和饲料样品植酸/总磷含量测量的简便方法。不需要通过阴离子交换色谱对植酸纯化，适合于大量样本分析
K-PYRUV
丙酮酸检测试剂盒
在啤酒、葡萄酒、果汁、食品和体液中丙酮酸分析
K-RAFGA
棉子糖/D-半乳糖检测试剂盒
快速测量植物材料和食品中棉子糖和半乳糖含量
K-RAFGL
棉子糖/蔗糖/D-半乳糖检测试剂盒
分析种子和种子粉中D-葡萄糖、蔗糖、棉子糖、水苏糖和毛蕊花糖含量。通过将棉子糖、水苏糖和毛蕊花糖酶解D-葡萄糖、D-果糖和半乳糖，从而测定葡萄糖含量来确定
K-SDAM
淀粉损伤检测试剂盒
谷物面粉中淀粉损伤的检测和分析
K-SUCGL
蔗糖/D-葡萄糖检测试剂盒
饮料、果汁、蜂蜜和食品中蔗糖和葡萄糖的分析
K-SUFRG
蔗糖/D-果糖/D-葡萄糖检测试剂盒
适用于植物和食品中蔗糖、D-葡萄糖和D-果糖的测定
K-TDFR
总膳食纤维检测试剂盒
总膳食纤维检测
K-TREH
海藻糖检测试剂盒
快速、可靠地检测食品、饮料和其它物料中海藻糖含量
K-URAMR
尿素/氨快速检测试剂盒
适用于水、饮料、乳制品和食品中尿素和氨的快速测定
K-URONIC
D-葡萄糖醛酸/D-半乳糖醛酸检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中六元糖醛酸含量（D-葡萄糖醛酸和D-半乳糖醛酸）
K-XYLOSE
D-木糖检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中D-木糖含量
K-YBGL
Beta葡聚糖[酵母和蘑菇]检测试剂盒
检测酵母和蘑菇制品中1,3:1,6-beta-葡聚糖和α-葡聚糖含量
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