TheL-Asparagine/L-Glutamine/Ammoniatestkitisanovelmethodforthespecific,convenient,costeffectiveandrapidmeasurementandanalysisofL-asparagine,L-glutamineandammoniaasacrylamideprecursorsinthefoodindustry,orascellculturemedia/supernatantcomponents,orinothermaterials.
ExtendedcofactorsstABIlity.Dissolvedcofactorsstablefor>1yearat4oC.
Oncolyticmeaslesvirusesproducedatdifferentscalesunderserum‐freeconditions.
UV-methodforthedeterminationofL-Asparagine,L-Glutamine
andAmmoniainpotatoes,foodstuffsandcellculturemedia
Principle:
(glutaminase)
(1)L-Glutamine+H2O→L-glutamate+NH4+
(microbialglutamatedehydrogenase)
(2)NH4++2-oxoglutarate+NADPH→L-glutamate+NADP++H2O
(asparaginase)
(3)L-Asparagine+H2O→L-aspartate+NH4+
Kitsize: *50assaysofeach
* Thenumberofmanualtestsperkitcanbedoubledifallvolumesarehalved.
ThiscanbereADIlyaccommodatedusingtheMegaQuantTM Wave
Spectrophotometer(D-MQWAVE).Method: Spectrophotometricat340nm
Reactiontime: ~20min
Detectionlimit: 0.50mg/L(L-asparagine)
0.54mg/L(L-glutamine)
0.06mg/L(ammonia)
Applicationexamples:
Potatoes,potatoproducts,vegetables,cerealsandothermaterials
(e.g.BIOLOGicalcultures,samples,etc.)
Methodrecognition: Novelmethod
Advantages
- Veryrapidreactionduetouseofuninhibitedglutamatedehydrogenase
- AllenzymessuppliedasstabilisedsUSPensions
- Onlykitavailable
- Verycosteffective
- Allreagentsstablefor>2yearsafterpreparation
- Mega-Calc™softwaretoolisavailablefromourwebsiteforhassle-freerawdataprocessing
- Standardincluded