
TheAspartametestkitisasimpleandreliablemethodforthespecificmeasurementandanalysisofAspartameinbeveragesandfoodstuffs.
Suitableformanual,auto-analyserandmicroplateformats.
EnhancementofrecombinantproteinproductioninEscherichiacolibycoproductionofaspartase.
UV-methodforthedeterminationofAspartame(andbreakdown
products)infoodstuffs,beveragesandothermaterials
Principle:
(pH12.5)
(1)Asp-Phe-O-Me→Asp-Phe+MeOH
(dipeptidaseM)
(2)Asp-Phe+H2O→L-aspartate+L-phenylalanine
(glutamate-oxaloacetatetransaminase)
(3)L-Aspartate+2-oxoglutarate→L-glutamate+oxaloacetate
(L-malatedehydrogenase)
(4)Oxaloacetate+NADH+H+→L-malate+NAD+
Kitsize: *50assays(manual)/500(microplate)
/500(auto-analyser)* Thenumberofmanualtestsperkitcanbedoubledifallvolumesarehalved.
ThiscanbereADIlyaccommodatedusingtheMegaQuantTM Wave
Spectrophotometer(D-MQWAVE).Method: Spectrophotometricat340nm
Reactiontime: ~5min
Detectionlimit: 0.57mg/L
Applicationexamples:
Softdrinks,artificialsweeteners,candies,mints,chewinggum,dietetic
products,jam,chocolateandothermaterials
Methodrecognition: Novelmethod
Advantages
- Verycosteffective
- Allreagentsstablefor>12monthsafterpreparation
- Onlyenzymatickitavailable
- Measuresaspartameandbreakdownproducts(L-aspartateandaspartameacid)
- Veryspecific
- Veryrapidreaction
- Mega-Calc™softwaretoolisavailablefromourwebsiteforhassle-freerawdataprocessing
- Standardincluded
- Suitableformanual,microplateandauto-analyserformats