
TheL-Glutamine/Ammonia(Rapid)testkitisanovelmethodforthespecific,convenient,costeffectiveandrapidmeasurementandanalysisofL-glutamineandammoniainculturemedia/supernatantsandothermaterials.
ExtendedcofactorsstABIlity.Dissolvedcofactorsstablefor>1yearat4oC.
AmutationinGDP-mannosepyrophosphorylasecausesconditionalhypersensitivitytoammonium,resultinginArabidopsisrootgrowthinhibition,alteredammoniummetabolism,andhormonehomeostasis.
MetabolicprofilingofhematopoieticstemandProgenitorcellsduringproliferationanddifferentiationintoredbloodcells.
UV-methodforthedeterminationofL-GlutamineandAmmonia
incellculturemedia,foodstuffsandothermaterials
Principle:
(glutaminase)
(1)L-Glutamine+H2O→L-glutamate+NH4+
(microbialglutamatedehydrogenase)
(2)NH4++2-Oxoglutarate+NADPH→L-glutamate+NADP++H2O
Kitsize: * 50assaysofeach(manual)/500assaysofeach(microplate)
* Thenumberofmanualtestsperkitcanbedoubledifallvolumesarehalved.
ThiscanbereADIlyaccommodatedusingtheMegaQuantTM Wave
Spectrophotometer(D-MQWAVE).Method: Spectrophotometricat340nm
Reactiontime: ~10min
Detectionlimit: 0.54mg/L(L-glutamine)
0.06mg/L(ammonia)
Applicationexamples:
Cellculturemediaandcultures,dietarysupplements,vegetables
andothermaterials(e.g.BIOLOGicalsamples,etc.)
Methodrecognition: Novelmethod
Advantages
- Veryrapidreactionduetouseofhighactivityglutaminaseanduninhibitedglutamatedehydrogense
- AllenzymessuppliedasstabilisedsUSPensions
- Onlyenzymatickitavailable
- Verycosteffective
- Allreagentsstablefor>2yearsafterpreparation
- Mega-Calc™softwaretoolisavailablefromourwebsiteforhassle-freerawdataprocessing
- Standardincluded