Highpurityrecombinant 1,2-α-L-Fucosidase(microbial)foruseinresearch,biochemicalenzymeassaysandinvitrodiagnosticanalysis.
EC3.2.1.63
CAZyFamily:GH95
CAS:37288-45-2
1,2-alpha-L-fucosidase;2-alpha-L-fucopyranosyl-beta-D-galactosidefucohydrolase
Recombinant.Fromamicrobialsource.
Insolution(Tris.HCl/NaCl/EDTA).
Suppliedat~12U/mL.
Specificactivity:
~160U/mg(37oC,pH6.5on2’-fucosyllactose)
StABIlity: > 3yearsat4oC.
ComparisonsofCaenorhaBDitisfucosyltransferasemutantsrevealamultiplicityofisomericN-glycanstructures.
AnalysisofInvertebrateandProtistN-Glycans.
DESCRIPTION
1,2-α-L-Fucosidase(microbial)
EC3.2.1.63
CAZyFamily:GH95
CAS: 37288-45-2
Synonyms:
1,2-alpha-L-fucosidase;2-alpha-L-fucopyranosyl-beta-D-galactosidefucohydrolase
Form:
Insolution(Tris.HCl/NaCl/EDTA).
Stability:
>3yearsat4oC.
Specificactivity:
~160U/mg(37oC,pH6.5on2’-fucosyllactose).
Unitdefinition:
OneUnitofα-1,2-L-fucosidaseactivityisdefinedastheamountofenzymerequiredtoreleaseoneμmolofα-L-fucoseperminutefrom2’-fucosyllactose(2mM)insodiumphosphatebuffer(100mM),pH6.5at37oC.
Specificity:
Highlyspecificfornon-reducingterminalL-fucoseresidueslinkedtoD-galactoseresiduesbya1,2-α-linkage.Doesnothydrolysep-nitrophenyl-α-L-fucopyranoside.
Applications:
Foruseinglycobiologyresearch.