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Megazyme/&alpha-(1-2,3,4,6)-D-甘露糖苷酶(温度为90°C时的Thermotoga maritima)/E-AMANTM/5单位
Highpurityα-Mannosidase(Thermotogamaritima)foruseinresearch,biochemicalenzymeassaysandinvitrodiagnosticanalysis.
EC3.2.1.24
CAZyFamily:GH38
CAS:9025-42-7
alpha-mannosidase;alpha-D-Mannosidemannohydrolase,alpha-D-mannopyranosidase
Recombinant.FromThermotogamaritima.
In3.2Mammoniumsulphate.
Suppliedat~71U/mL.
Specificactivity:
4.5U/mgprotein(onpNP-α-D-mannopyranoside)atpH6.0and90oC;
~0.2U/mgat40oC.
StABIlity:>4years4oC.
DESCRIPTION
α-(1-2,3,4,6)-D-Mannosidase(Thermotogamaritima)
EC3.2.1.24
CAZyFamily:GH38
CAS:9025-42-7
Synonyms:
alpha-mannosidase;alpha-D-Mannosidemannohydrolase,alpha-D-mannopyranosidase
Form:
In3.2Mammoniumsulphate.
Stability:
>4years4oC.
Specificactivity:
4.5U/mgprotein(onpNP-α-D-mannopyranoside)atpH6.0and90oC;
~0.2U/mgat40oC.
Unitdefinition:
OneUnitofα-D-Mannosidaseactivityisdefinedastheamountofenzymerequiredtoreleaseoneµmoleofp-nitrophenol(pNP)perminutefromp-nitrophenyl-α-D-mannopyranoside(2mM)inMESbuffer(100mM)andCoCl2(1mM)atpH6.0atthetemperaturesindicated.
Specificity:
Broadspecificity;hydrolysisofterminalnon-reducingalpha-D-mannoseinalpha-D-mannosides.
Applications:
ForuseinglycoBIOLOGyresearch.