Highpurityrecombinantexo-α-sialidase(Clostridiumperfringens)foruseinresearch,biochemicalenzymeassaysandinvitrodiagnosticanalysis.
EC3.2.1.18
CAZyFamily:GH33
CAS:9001-67-6
exo-alpha-sialidase;acetylneuraminylhydrolase
Recombinant.FromClostridiumperfringens.
In3.2Mammoniumsulphate.
Suppliedat~500U/mL.
Specificactivity:
~140U/mg(37oC,pH7.0onpNP-α-D-N-acetylneuraminicacid).
StABIlity:>2yearsat4oC.
Customquantitiesandformulationsavailableuponrequest.
DESCRIPTION
exo-α-Sialidase(Clostridiumperfringens)
EC 3.2.1.18
CAZyFamily:GH33
CAS: 9001-67-6
Synonyms:
exo-alpha-sialidase;acetylneuraminylhydrolase
Form:
In3.2Mammoniumsulphate.
Stability:
>2yearsat4oC.
Specificactivity:
~140U/mg(37oC,pH7.0onpNP-α-D-N-acetylneuraminicacid).
Unitdefinition:
OneUnitofsialidaseactivityisdefinedastheamountofenzymerequiredtoreleaseoneµmoleofp-nitrophenolperminutefrompNP-α-D-N-acetylneuraminicacid(1mM)insodiumphosphatebuffer(100mM),pH7.0at37oC.
Specificity:
Hydrolysisofunbranched,non-reducingterminalα-2,3-linked,α-2,6-linked>>α-2,8-linkedN-acetylneuraminicacid(NANA;Neu5Ac)residuesfromglycoproteinsandoligosaccharidesofglycoconjugates.
Applications:
Applicationsinclinicalchemistry,glycoBIOLOGyandcarbohydrateresearch.