Megazyme/Amylazyme Red Tablets/T-AMZRD-1000T/1,000 Tablets
商品编号：
T-AMZRD-1000T
品牌：
Megazyme INC
市场价：
¥19608.00
美元价：
11764.80
产品分类：
反应底物
公司分类：
Reaction_substrate
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
HighpuritydyedandcrosslinkedAmylazymeRedtabletsforthemeasurementofenzymeactivity,forresearch,biochemicalenzymeassaysandinvitrodiagnosticanalysis.
Ahighlysensitivesubstrateforthemeasurementofα-amylase.
Newchromogenicsubstratesfortheassayofalpha-amylaseand(1-4)-β-D-glucanase.
McCleary,B.V.(1980).CarbohydrateResearch,86(1),97-104.
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Newchromogenicsubstrateshavebeendevelopedforthequantitativeassayofalpha-amylaseand(1→4)-β-D-glucanase.Thesewerepreparedbychemicallymodifyingamyloseorcellulosebeforedyeing,toincreasesolubility.Afterdyeing,thesubstrateswereeithersolubleorcouldbereADIlydispersedtoformfine,gelatinoussUSPensions.Assaysbasedontheuseofthesesubstratesaresensitiveandhighlyspecificforeitheralpha-amylaseor(1→4)-β-D-glucanase.Themethodofpreparationcanalsobeappliedtoobtainsubstratesforotherendo-hydrolases.
Comparisonofendolytichydrolasesthatdepolymerise1,4-β-D-mannan,1,5-α-L-arABInanand1,4-β-D-galactan.
McCleary,B.V.(1991).“EnzymesinBiomassConversion”,(M.E.HimmelandG.F.Leatham,Eds.),ACSSymposiumSeries460,Chapter34,pp.437-449.AmericanChemicalSociety,Washington.
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Hydrolysisofmannan-typepolysaccharidesbyβ-mannanaseisdependentonsubstitutiononandwithinthemain-chainaswellasthesourceoftheβ-mannanaseemployed.Characterisationofreactionproductscanbeusedtodefinethesub-sitebindingrequirementsoftheenzymesaswellasthefine-structuresofthepolysaccharides.Actionofendo-arabinanaseandendo-galactanaseonarabinansandarabinogalactansisdescribed.Specificassaysforendo-arabinanaseandarabinan(infruit-juiceconcentrates)arereported.
Measurementofpolysaccharidedegradingenzymesusingchromogenicandcolorimetricsubstrates.
McCleary,B.V.(1991).ChemistryinAustralia,58,398-401.
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Enzymicdegradationofcarbohydratesisofmajorsignificanceintheindustrialprocessingofcerealsandfruits.Intheproductionofbeer,barleyisgerminatedunderwelldefinedconditions(malting)toinducemaximumenzymesynthesiswithminimumrespirationofreservecarbohydrates.Thegrainsaredriedandthenextractedwithwaterundercontrolledconditions.Theamylolyticenzymessynthesizedduringmalting,aswellasthosepresentintheoriginalbarley,convertthestarchreservestofermentablesugars.Otherenzymesactonthecellwallpolysaccharides,mixed-linkageβ-glucanandarabinoxylan,reducingtheviscosityandthusaidingfiltration,andreducingthepossibilityofsubsequentprecipitationofpolymericmaterial.Inbaking,β-amylaseandα-amylasegivecontrolleddegradationofstarchtofermentablesugarssoastosustainyeastgrowthandgasproduction.Excessquantitiesofα-amylaseintheflourresultinexcessivedegradationofstarchduringbakingwhichinturngivesastickycrumbtextureandsubsequentproblemswithbreadslicing.Juiceyieldfromfruitpulpissignificantlyimprovedifcell-walldegradingenzymesareusedtodestroythethree-dimensionalstructureandwaterbindingcapacityofthepecticpolysaccharidecomponentsofthecellwalls.Problemsofroutineandreliableassayofcarbohydratedegradingenzymesinthepresenceofhighlevelsofsugarcompoundsareexperiencedwithsuchindustrialprocess.
Measurementofα-amylaseincereal,foodandfermentationproducts.
McCleary,B.V.&Sturgeon,R.(2002).CerealFoodsWorld,47(7),299-310.
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InGeneral,thedevelopmentofmethodsformeasuringα-amylaseispioneeredintheclinicalchemistryfieldandthentranslatedtootherindustries,suchasthecerealsandfermentationindustries.Inmanyinstances,thistransferoftechnologyhasbeendifficultorimpossIBLetoachieveduetothepresenceofinterferingenzymesorsugarsandtodifferencesinthepropertiesoftheenzymesbeinganalysed.Thisarticledescribesmanyofthecommonlyusedmethodsformeasuringα-amylaseinthecereals,food,andfermentationindustriesanddiscussessomeoftheadvantagesandlimitationsofeach.
Comparisonofshort-wavelengthinfrared(SWIR)hyperspectralimagingsystemwithanFT-NIRspectrophotometerforpredictingalpha-amylaseactivitiesinindividualCanadianWesternRedSpring(CWRS)wheatkernels.
Xing,J.,Symons,S.,Hatcher,D.&Shahin,M.(2011).BiosystemsEngineering,108(4),303-310.
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Alpha-amylaseactivityinindividualCanadianWesternRedSpring(CWRS)wheatkernelswaspredictedusingspectralinformationacrossthewavelengthregion1235–2450nm.ReflectancespectrawerecollectedfromanSWIR(short-wavelengthinfrared)hyperspectralimagingsystemandabsorbancespectrawererecordedfromanFouriertransformnear-infrared(FT-NIR)spectrometeronthesamekernels.Thepartialleastsquares(PLS)regressiontechniquewasusedtomodelthealpha-amylaseenzymeactivitylevelstothespectralinformation.Thepredictionaccuracyvariedwiththepre-processingmethodsappliedtotheregressorandregressand.Thehighestcoefficientofdetermination(r2)valueobtainedfromtheSWIRhyperspectralimagingsystemwas0.88and0.82fromtheFT-NIRinstrument.Theimagingapproachwasmoresuccessfulbecauseitalsohadtheadvantageofbeingabletolocalisetheregionwherespectrawereextractedfrom.
Theobjectivemeasurementofalpha-amylaseinwheatkernelsusingspectralimaging.
Symons,S.,Xing,J.,Shahin,M.&Hatcher,D.(2010,September).WorldAutomationCongress(WAC),257-262.
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Whenwheatkernelsarewettedintheheadpriortoharvest,thegerminationprocessesareinitiated.Thesymptomsrangefromnoobviousvisiblesignsofenzymeactivationtogrosskerneldisfiguration.Alpha-amylase,astarchdegradingenzymeisthemostprevalentoftheactivatedenzymesintheearlystagesofgerminationandmaycausesignificantend-productqualityloss.Currentanalyticaltechniquesdonotprovidearapidsystemforestimatingindividualkernelsproutdamage.Wehavedevelopedanobjectiveapproachusingnear-infraredspectra(1100-2400nm)fromahyperspectralcameratopredictα-amylaselevelsofindividualkernelsintwoclassesofCanadianwheat.Multivariatemodelinggave,anR2ofupto0.69forpredictingindividualkernelα-amylaselevels.Usingthehyperspectraldata,amultispectralmodelpredictedα-amylaseactivitylevelsofgreaterthan1SKUunit/gwithabetterthan90%accuracy.Atthislevel,thereisnovisiblesignofkernelsprouting.
UsingaShortWavelengthInfrared(SWIR)hyperspectralimagingsystemtopredictalphaamylaseactivityinindividualCanadianwesternwheatkernels.
Xing,J.,VanHung,P.,Symons,S.,Shahin,M.&Hatcher,D.(2009).SensingandInstrumentationforFoodQualityandSafety,3(4),211-218.
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Sproutdamage(pre-harvestgermination)inwheatresultsinhighlydeleteriouseffectsonend-productquality.Alpha-amylase,thepre-dominantenzymeintheearlystageofsproutinghasthemostdamagingeffect.ThispaperintroducesanewmethodusingaSWIRhyperspectralimagingsystem(1000–2500nm)topredicttheα-amylaseactivityofindividualwheatkernels.TwoclassesofCanadianwheat,CanadaWesternRedSpring(CWRS)andCanadaWesternAmberDurum(CWAD),withsamplesofdifferingdegreesofsproutdamagewereinvestigated.Individualkernelswerefirstimagedwiththehyperspectralimagingsystemandthentheα-amylaseactivityofeachkernelwasdeterminedanalytically.Individualkernelα-amylaseactivitypredictionwassignificant(R20.54and0.73)forCWADandCWRS,respectivelyusingPartialLeastSquareregressiononthehyperspectraldata.AclassificationmethodisproposedtoseparateCWRSkernelswithhighα-amylaseactivitylevelfromthosewithlowα-amylaseactivitygivinganaccuracyofabove80%.Thisworkshowsthathyper/multi-spectralimagingtechniquescanbeusedforrapidlypredictingtheα-amylaseactivityofindividualkernels,detectingsproutingatearlystage.
Anovelα-amylasegeneistransientlyupregulatedduringlowtemperatureexposureinapplefruit.
Wegrzyn,T.,Reilly,K.,Cipriani,G.,Murphy,P.,Newcomb,R.,Gardner,R.&MacRae,E.(2000).EuropeanJournalofBiochemistry,267(5),1313-1322.
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Anα-amylasegeneproductwasisolatedfromapplefruitbyreverse-transcriptasePCRusingredundantprimers,followedby5′and3′RACE.Thegeneisamemberofasmallgenefamily.Itencodesaputative46.9kDaproteinthatismostsimilartoanα-amylasegenefrompotato(GenBankaccessionM79328).Inapplefruitthisnewgenewasexpressedatlowlevels,asdetectedbyreverse-transcriptasePCR,inanumberofplanttissuesandduringfruitdevelopment.HighestlevelsofmRNAforthistranscriptwereobserved3to9daysafterplacingapplefruitat0.5°C.Phylogeneticanalysisofaminoacidsequenceplacesthepotatoandappleproteinsasadistinctandseparatenewsubgroupwithintheplantα-amylases,whichappearstohavedivergedpriortothesplitbetweenmonocotyledonousanddicotyledonousplants.Thesetwodivergentα-amylaseslackthestandardsignalpeptidestructuresfoundinallotherplantα-amylases,andhavesequencedifferenceswithintheB-domainandC-domain.However,comparisonswithstructuresofknownstarchhydrolasessuggestthatthesedifferencesareunlikelytoaffecttheenzymaticα-1,4-amylasefunctionoftheprotein.Thisisthefirstreportofupregulationofadicotyledonousα-amylaseinresponsetolowtemperature,andconfirmsthepresenceofanewfamilyofα-amylasesinplants.
品牌介绍
Megazyme品牌产品简介
来源：作者：人气：2149发表时间：2016-05-19 10:59:00【大 中 小】
Megazyme是一家全球性公司，专注于开发和提供用于饮料、谷物、乳制品、食品、饲料、发酵、生物燃料和葡萄酒产业用的分析试剂、酶和检测试剂盒。Megazyme的许多检测试剂盒产品已经为众多官方科学协会（包括AOAC, AACC , RACI, EBC和ICC等），经过严格的审核，批准认证为官方标准方法，确保以准确、可靠、定量和易于使用的测试方法，满足客户的质量诉求。
Megazyme的主要产品线包括：
◆ 检测试剂盒
◆ 酶
◆ 酶底物
◆ 碳水化合物
◆ 化学品/仪器
官网地址：http://www.megazyme.com
检测试剂盒特色产品：
货号
中文品名
用途
K-ACETAF
乙酸[AF法]检测试剂盒
酶法定量分析乙酸最广泛使用的方法
K-ACHDF
可吸收糖/膳食纤维检测试剂盒
酒精沉淀法测定膳食纤维
K-AMIAR
氨快速检测试剂盒
用于包括葡萄汁、葡萄酒以及其它食品饮料样品中氨含量的快速检测分析。
K-AMYL
直链淀粉/支链淀粉检测试剂盒
谷物淀粉和而粉中直链淀粉/支链淀粉比例和含量检测
K-ARAB
阿拉伯聚糖检测试剂盒
果汁浓缩液中阿拉伯聚糖的检测
K-ASNAM
L-天冬酰胺/L-谷氨酰胺和氨快速检测试剂盒
用于食品工业中丙烯酰胺前体、细胞培养基、以及上清液组分中、L-天冬酰胺，谷氨酰胺和氨的检测分析
K-ASPTM
阿斯巴甜检测试剂盒
专业用于测定饮料和食品中阿斯巴甜含量，操作简单
K-BETA3
β-淀粉酶检测试剂盒
适用于麦芽粉中β-淀粉酶的测定
K-BGLU
混合键β-葡聚糖检测试剂盒
测定谷物、荞麦粉、麦汁、啤酒及其它食品中混合键β-葡聚糖(1,3:1,4-β-D-葡聚糖)的含量
K-CERA
α-淀粉酶检测试剂盒
谷物和发酵液（真菌和细菌）中α-淀粉酶的分析测定
K-CITR
柠檬酸检测试剂盒
快速、可靠地检测食品、饮料和其它物料中柠檬酸（柠檬酸盐）含量
K-DLATE
乳酸快速检测试剂盒
快速、特异性检测饮料、肉类、奶制品和其它食品中L-乳酸和D-乳酸(乳酸盐）含量
K-EBHLG
酵母β-葡聚糖酶检测试剂盒
用于测量和分析酵母中1,3:1,6?-β-葡聚糖，也可以检测1,3-葡聚糖
K-ETSULPH
总亚硫酸检测试剂盒
测定葡萄酒、饮料、食品和其他物料中总亚硫酸含量（按二氧化硫计）的一种简单，高效，可靠的酶法检测方法
K-FRGLMQ
D-果糖/D-葡萄糖[MegaQuant法]检测试剂盒
适用于使用megaquant?色度计（505nm下）测定葡萄、葡萄汁和葡萄酒中D-果糖和D-葡萄糖的含量。
K-FRUC
果聚糖检测试剂盒
含有淀粉、蔗糖和其他糖类的植物提取物和食品中果聚糖的含量测定。
K-FRUGL
D-果糖/D-葡萄糖检测试剂盒
对植物和食品中果糖或葡萄糖含量的酶法紫外分光测定。
K-GALM
半乳甘露聚糖检测试剂盒
食品和植物产品中半乳甘露聚糖的含量检测
K-GLUC
D-葡萄糖[GOPOD]检测试剂盒
谷物提取物中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUHK
D-葡萄糖[HK]检测试剂盒
植物和食品中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUM
葡甘聚糖检测试剂盒
植物和食品中葡甘聚糖的含量测定。
K-INTDF
总膳食纤维检测试剂盒
总膳食纤维特定检测和分析
K-LACGAR
乳糖/D-半乳糖快速检测试剂盒
用于快速检测食品和植物产品中乳糖、D-半乳糖和L-阿拉伯糖
K-LACSU
乳糖/蔗糖/D-葡萄糖检测试剂盒
混合面粉和其它物料中蔗糖、乳糖和D-葡萄糖的测定
K-LACTUL
乳果糖检测试剂盒
特异性、快速和灵敏测量奶基样品中乳果糖含量
K-MANGL
D-甘露糖/D-果糖/D-葡萄糖检测试剂盒
适合测定植物产品和多糖酸性水解产物中D-甘露糖含量
K-MASUG
麦芽糖/蔗糖/D-葡萄糖检测试剂盒
在植物和食品中麦芽糖，蔗糖和葡萄糖的含量检测
K-PECID
胶质识别检测试剂盒
食品配料中果胶的鉴别
K-PHYT
植酸(总磷)检测试剂盒
食品和饲料样品植酸/总磷含量测量的简便方法。不需要通过阴离子交换色谱对植酸纯化，适合于大量样本分析
K-PYRUV
丙酮酸检测试剂盒
在啤酒、葡萄酒、果汁、食品和体液中丙酮酸分析
K-RAFGA
棉子糖/D-半乳糖检测试剂盒
快速测量植物材料和食品中棉子糖和半乳糖含量
K-RAFGL
棉子糖/蔗糖/D-半乳糖检测试剂盒
分析种子和种子粉中D-葡萄糖、蔗糖、棉子糖、水苏糖和毛蕊花糖含量。通过将棉子糖、水苏糖和毛蕊花糖酶解D-葡萄糖、D-果糖和半乳糖，从而测定葡萄糖含量来确定
K-SDAM
淀粉损伤检测试剂盒
谷物面粉中淀粉损伤的检测和分析
K-SUCGL
蔗糖/D-葡萄糖检测试剂盒
饮料、果汁、蜂蜜和食品中蔗糖和葡萄糖的分析
K-SUFRG
蔗糖/D-果糖/D-葡萄糖检测试剂盒
适用于植物和食品中蔗糖、D-葡萄糖和D-果糖的测定
K-TDFR
总膳食纤维检测试剂盒
总膳食纤维检测
K-TREH
海藻糖检测试剂盒
快速、可靠地检测食品、饮料和其它物料中海藻糖含量
K-URAMR
尿素/氨快速检测试剂盒
适用于水、饮料、乳制品和食品中尿素和氨的快速测定
K-URONIC
D-葡萄糖醛酸/D-半乳糖醛酸检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中六元糖醛酸含量（D-葡萄糖醛酸和D-半乳糖醛酸）
K-XYLOSE
D-木糖检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中D-木糖含量
K-YBGL
Beta葡聚糖[酵母和蘑菇]检测试剂盒
检测酵母和蘑菇制品中1,3:1,6-beta-葡聚糖和α-葡聚糖含量
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