Megazyme/阿拉伯酶片/T-ARZ-200T/200片
商品编号：
T-ARZ-200T
品牌：
Megazyme INC
市场价：
¥5736.00
美元价：
3441.60
产品分类：
反应底物
公司分类：
Reaction_substrate
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
HighpuritydyedandcrosslinkedArABInazymetabletsforthemeasurementofenzymeactivity,forresearch,biochemicalenzymeassaysandinvitrodiagnosticanalysis.
Fortheassayofendo-1,5-α-L-arabinanase.ContainingAZCL-Debranchedarabinan.
Cloningandcharacterizationofarabinoxylanarabinofuranohydrolase-D3(AXHd3)fromBifidobacteriumadolescentisDSM20083.
VandenBroek,L.A.M.,Lloyd,R.M.,Beldman,G.,Verdoes,J.C.,McCleary,B.V.&Voragen,A.G.J.(2005).AppliedMicroBIOLOGyandBiotechnology,67(5),641-647.
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Arabinoxylanarabinofuranohydrolase-D3(AXHd3)fromBifidobacteriumadolescentisreleasesonlyC3-linkedarabinoseresiduesfromdouble-substitutedxyloseresidues.AgenomiclibraryofB.adolescentisDSM20083wasscreenedforthepresenceoftheaxhD3gene.TwoplasmidswereidentifiedcontainingpartoftheaxhD3gene.ThenucleotidesequenceswerecombinedandthreeopenreADIngframes(ORFs)werefound.ThefirstORFshowedhighhomologywithxylanasesbelongingtofamily8oftheglycosidehydrolasesandthisgenewasdesignatedxylA.ThesecondORFwastheaxhD3genebelongingtoglycosidehydrolasefamily43.Thethird(partial)ORFcodedforaputativecarboxylesterase.TheaxhD3genewasclonedandexpressedinEscherichiacoli.SeveralsubstrateswereemployedinthebiochemicalcharacterizationofrecombinantAXHd3.Theenzymeshowedthehighestactivitytowardwheatarabinoxylanoligosaccharides.Inaddition,β-xylanasefromTrichodermasp.wasabletodegradesolublewheatarabinoxylanpolymertoahigherextent,afterpretreatmentwithrecombinantAXHd3.ArabinoxylanoligosaccharidesincubatedwithacombinationofrecombinantAXHd3andanα-L-arabinofuranosidasefromAspergillusnigerdidnotresultinahighermaximalreleaseofarabinosethanincubationwiththeseenzymesseparately.
ExtracellulararabinasesinAspergillusnidulans:theeffectofdifferentcremutationsonenzymelevels.
VanderVeen,P.,ArstJr,H.N.,Flipphi,M.J.&Visser,J.(1994).ArchivesofMicrobiology,162(6),433-440.
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Theregulationofthesynthesesoftwoarabinan-degradingextracellularenzymesandseveralintracellularL-arabinosecatabolicenzymeswasexaminedinwild-typeandcarboncatabolitederepressedmutantsofAspergillusnidulans.α-L-ArabinofuranosidaseB,endoarabinase,L-arabinosereductase,L-arabitoldehydrogenase,xylitoldehydrogenase,andL-xylulosereductasewereallinducIBLetovaryingdegreesbyL-arabinoseandL-arabitolandsubjecttocarboncataboliterepressionbyD-glucose.WiththeexceptionofL-xylulosereductase,allwereclearlyunderthecontrolofcreA,anegative-actingwidedomainregulatorygenemediatingcarboncataboliterepression.MeasurementsofintracellularenzymeactivitiesandofintracellularconcentrationsofarabitolandxylitolinmyceliagrownonD-glucoseinthepresenceofinducerindicatedthatcarboncataboliterepressiondiminishes,butdoesnotpreventuptakeofinducer.MutationsincreAresultedinanapparently,insomeinstancesverymarked,elevatedinducibility,perhapsreflectinganelementof“self”cataboliterepressionbytheinducingsubstrate.creAmutationsalsoresultedincarboncatabolitederepressiontovaryingdegrees.TheregulatoryeffectsofamutationincreBandincreC,twogeneswhoserolesareunclear,butlikelytobeindirect,were,whenobservable,moremodest.AswithpreviousdatashowingtheeffectofcreAmutationsonstructuralgeneexpression,therewerestrikinginstancesofphenotypicvariationamongstcreAmutantallelesandthisvariationfollowednodiscerniblepattern,i.e.itwasnon-hierarchical.Thisfurthersupportsmoleculardataobtainedelsewhere,indicatingadirectroleforcreAinregulatingstructuralgeneexpression,andextendstherangeofactivitiesundercreAcontrol.
Molecularcloning,expressionandstructureoftheendo-1,5-α-L-arabinasegeneofAspergillusniger.
Flipphi,M.J.A.,Panneman,H.,vanderVeen,P.,Visser,J.&deGraaff,L.H.(1993).AppliedMicrobiologyandBiotechnology,40(2-3),318-326.
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Secretionofendo-1,5-α-L-arabinaseA(ABNA)byanAspergillusnigerxylulosekinasemutantuponmyceliumtransfertomediumcontainingL-arabitolwasimmunochemicallyfollowedwithtimetomonitoritsinductionprofile.ACDNAexpressionlibrarywasmadefrompolyA+RNAisolatedfromtheinducedmycelium.ThislibrarywasimmunochemicallyscreenedandoneABNAspecificcloneemerged.ThecorrespondingabnAgenewasisolatedfromanA.nigergenomiclibrary.UponSouthernblotanalysis,a3.1-kbHindIIIfragmentwasidentifiedandsubclonedtoresultinplasmidpIM950.Bymeansofco-tranformationusingtheA.nigerpyrAgeneasselectionMarker,thegenewasintroducedinbothA.nigerandA.nidulansuridineauxotrophicmutants.PrototrophicA.nigerandA.nidulanstransformantsoverproducedA.nigerABNAupongrowthinmediumcontainingsugarbeetpulpasthesolecarbonsource,therebyestablishingtheidentityandfunctionalityoftheclonedgene.TheDNAsequenceofthecompleteHindIIIfragmentwasdeterminedandthestructureoftheabnAgeneaswellasofitsdeducedgeneproductwereanalysed.GeneabnAcontainsthreeintronswithinitsstructuralregionandcodesforaproteinof321aminoacids.Signalpeptideprocessingresultsinamatureproteinof302aminoacidswithadeducedmolecularmassof32.5kDa.A.nigerabnAisthefirstgeneencodinganABNtobeisolatedandcharacterized.
EfficientproductionofprotopectinasesbyBacillussubtilisusingmediumbasedonsoybeanflour.
Matsumoto,T.,Sugiura,Y.,Kondo,A.&Fukuda,H.(2000).BiochemicalEngineeringJournal,6(2),81-86.
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Wehavedevelopedaculturesystemforefficientproductionofprotopectinases(PPases)byBacillussubtilis.PPaseshowsthepectin-releasingactivityandisexpectedtobeutilizedintheenzymaticcottonscouring.B.subtilisIFO3134wascultivatedusingdefattedsoybeanflourasamaincomponentofculturemedia.ThisstrainproducedthreedifferenttypesofPPases,namely,PPase-C,-Nand-Rperformingendo-arabinaseactivity,pectate-lyaseactivityandpectin-lyaseactivity,respectively.Theeffectsofalkalinesolubilizationandautoclavetreatmentstoextractnutrientsfromsoybeanflourandinitialsoybeanflourconcentration(20–80g/l)onproductionofPPasesinbatchfermentationwereinvestigated.AlkalinesolubilizationofsoybeanflourwithNaOHremarkablyreducedenzymeproductivity.Inaddition,ahigherinitialconcentrationofsoybeanflourreducedtheenzymeproductivityofcells.Thepectin-releasingactivitywasthelargestandreachedupto2200–2400U/ml,whentheculturemediumcontaininganinitialsoybeanflourconcentrationof40g/lwasautoclavedfor45–60minwithoutalkalinesolubilizationtreatment.
AntisensesilencingofthecreAGeneinAspergillusnidulans.
Bautista,L.F.,Aleksenko,A.,Hentzer,M.,Santerre-Henriksen,A.&Nielsen,J.(2000).AppliedandEnvironmentalMicrobiology,66(10),4579-4581.
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AntisenseexpressionofaportionofthegeneencodingthemajorcarboncataboliterepressorCREAinAspergillusnidulansresultedinasubstantialincreaseinthelevelsofglucose-repressibleenzymes,bothendogenousandheterologous,inthepresenceofglucose.Thederepressioneffectwasapproximatelyone-halfofthatachievedinanullcreAmutant.Unlikeresultsforthatmutant,however,growthparametersandcolonymorphologyintheantisensetransformantswerenotaffected.
ArabinasegeneexpressioninAspergillusniger:indicationsforcoordinatedregulation.
Flipphi,M.J.A.,Visser,J.,vanderVeen,P.&deGraaff,L.H.(1994).Microbiology,140(10),2673-2682.
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AspergillusnigersecretesthreeglycosylatedglycosylhydrolaseswhichareinvolvedindegradationoftheplantcellwallpolysaccharideL-arabinan:α-L-arabinofuranosidases(ABF)AandB,andendo-1,5-α-L-arabinase(ABN)A.ThenucleotidesequenceofthepreviouslyclonedgeneencodingABFA(abfA)fromA.nigerwasdetermined.Thecodingregioncontainssevenintrons.MatureABFAcomprises603aminoacidswithamolecularmassof65.4kDaasdeducedfromthenucleotidesequence.ThesecretedenzymeisN-glycosylated.TheprimarystructuresofthethreeA.nigerarabinasescharacterizedlacksimilarity.RegulationofarabinaseexpressionuponinductionbysugarbeetpulpandbyL-arabitolwasstudiedasafunctionoftime.Thiswasdoneinwild-typeA.nigeraswellasintransformantscarryingmultiplecopiesofeitheroneoftheABF-encodinggenes.EacharabinasegenerespondeddifferentlyuponamycelialtransfertoL-arabitol-containingmedium.ExtracopiesofabfAorabfBledtoadecreasedexpressionlevelofABNA,thoughtherepressionelicitedbyabfBisstrongerandmorepersistentthanthateffectedbyabfA.MultiplecopiesofbothabfgenesinfluenceexpressionoftheotherABFsimilarly,buttoafarlesspronounceddegreethantheyaffectABNAsynthesis.Fourputativepromoterelements,sharedbyallthreearabinasegenes,couldbeinvolvedincoordinationofL-arabinandegradationbyA.niger.
RecombinantexpressionandcharacterizationofXynDfromBacillussubtilissubsp.subtilisATCC6051:aGH43arabinoxylanarabinofuranohydrolase.
Bourgois,T.M.,VanCraeyveld,V.,VanCampenhout,S.,Courtin,C.M.,Delcour,J.A.,Robben,J.&Volckaert,G.(2007).AppliedMicrobiologyandBiotechnology,75(6),1309-1317.
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ThecompletegenomesequenceofBacillussubtilisrevealsthatsequencesencodingseveralhemicellulasesareco-localisedwithagene(xynD)encodingaputativefamily43glycosidehydrolasethathasnotyetbeencharacterised.Inthiswork,xynDhasbeenisolatedfromgenomicDNAofB.subtilissubsp.subtilisATCC6051andclonedforcytoplasmaticexpressioninEscherichiacoli.RecombinantXynD(rXynD)waspurifiedusingion-exchangechromatographyandgelpermeationchromatography.Theenzymehadamolecularmassofapproximately52kDa,ap/above9.0andreleasesα-L-arabinosefromarabinoxylo-oligosaccharidesaswellasarabinoxylanpolymerswithvaryingdegreeofsubstitution.Usingpara-nitrophenyl-α-L-arabinofuranosideassubstrate,maximumactivitywasobservedatpH5.6and45°C.TheenzymeretaineditsactivityoveralargepHrange,whileactivitywaslostafterpre-incubationabove50°C.Gas–liquidchromatographyandprotonnuclearmagneticresonancespectrometryanalysisindicatedthatrXynDspecificallyreleasesarabinofuranosylgroupsfrommono-substitutedC-(O)-2andC-(O)-3xylopyranosylresiduesonthexylanbackbone.AsrXynDdidnotdisplayendoxylanase,xylosidaseorarabinanaseactivityandwasinactiveonarabinan,weconcludethatthisenzymeisbestdescribedasanarabinoxylanarabinofuranohydrolase.
品牌介绍
Megazyme品牌产品简介
来源：作者：人气：2149发表时间：2016-05-19 10:59:00【大 中 小】
Megazyme是一家全球性公司，专注于开发和提供用于饮料、谷物、乳制品、食品、饲料、发酵、生物燃料和葡萄酒产业用的分析试剂、酶和检测试剂盒。Megazyme的许多检测试剂盒产品已经为众多官方科学协会（包括AOAC, AACC , RACI, EBC和ICC等），经过严格的审核，批准认证为官方标准方法，确保以准确、可靠、定量和易于使用的测试方法，满足客户的质量诉求。
Megazyme的主要产品线包括：
◆ 检测试剂盒
◆ 酶
◆ 酶底物
◆ 碳水化合物
◆ 化学品/仪器
官网地址：http://www.megazyme.com
检测试剂盒特色产品：
货号
中文品名
用途
K-ACETAF
乙酸[AF法]检测试剂盒
酶法定量分析乙酸最广泛使用的方法
K-ACHDF
可吸收糖/膳食纤维检测试剂盒
酒精沉淀法测定膳食纤维
K-AMIAR
氨快速检测试剂盒
用于包括葡萄汁、葡萄酒以及其它食品饮料样品中氨含量的快速检测分析。
K-AMYL
直链淀粉/支链淀粉检测试剂盒
谷物淀粉和而粉中直链淀粉/支链淀粉比例和含量检测
K-ARAB
阿拉伯聚糖检测试剂盒
果汁浓缩液中阿拉伯聚糖的检测
K-ASNAM
L-天冬酰胺/L-谷氨酰胺和氨快速检测试剂盒
用于食品工业中丙烯酰胺前体、细胞培养基、以及上清液组分中、L-天冬酰胺，谷氨酰胺和氨的检测分析
K-ASPTM
阿斯巴甜检测试剂盒
专业用于测定饮料和食品中阿斯巴甜含量，操作简单
K-BETA3
β-淀粉酶检测试剂盒
适用于麦芽粉中β-淀粉酶的测定
K-BGLU
混合键β-葡聚糖检测试剂盒
测定谷物、荞麦粉、麦汁、啤酒及其它食品中混合键β-葡聚糖(1,3:1,4-β-D-葡聚糖)的含量
K-CERA
α-淀粉酶检测试剂盒
谷物和发酵液（真菌和细菌）中α-淀粉酶的分析测定
K-CITR
柠檬酸检测试剂盒
快速、可靠地检测食品、饮料和其它物料中柠檬酸（柠檬酸盐）含量
K-DLATE
乳酸快速检测试剂盒
快速、特异性检测饮料、肉类、奶制品和其它食品中L-乳酸和D-乳酸(乳酸盐）含量
K-EBHLG
酵母β-葡聚糖酶检测试剂盒
用于测量和分析酵母中1,3:1,6?-β-葡聚糖，也可以检测1,3-葡聚糖
K-ETSULPH
总亚硫酸检测试剂盒
测定葡萄酒、饮料、食品和其他物料中总亚硫酸含量（按二氧化硫计）的一种简单，高效，可靠的酶法检测方法
K-FRGLMQ
D-果糖/D-葡萄糖[MegaQuant法]检测试剂盒
适用于使用megaquant?色度计（505nm下）测定葡萄、葡萄汁和葡萄酒中D-果糖和D-葡萄糖的含量。
K-FRUC
果聚糖检测试剂盒
含有淀粉、蔗糖和其他糖类的植物提取物和食品中果聚糖的含量测定。
K-FRUGL
D-果糖/D-葡萄糖检测试剂盒
对植物和食品中果糖或葡萄糖含量的酶法紫外分光测定。
K-GALM
半乳甘露聚糖检测试剂盒
食品和植物产品中半乳甘露聚糖的含量检测
K-GLUC
D-葡萄糖[GOPOD]检测试剂盒
谷物提取物中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUHK
D-葡萄糖[HK]检测试剂盒
植物和食品中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUM
葡甘聚糖检测试剂盒
植物和食品中葡甘聚糖的含量测定。
K-INTDF
总膳食纤维检测试剂盒
总膳食纤维特定检测和分析
K-LACGAR
乳糖/D-半乳糖快速检测试剂盒
用于快速检测食品和植物产品中乳糖、D-半乳糖和L-阿拉伯糖
K-LACSU
乳糖/蔗糖/D-葡萄糖检测试剂盒
混合面粉和其它物料中蔗糖、乳糖和D-葡萄糖的测定
K-LACTUL
乳果糖检测试剂盒
特异性、快速和灵敏测量奶基样品中乳果糖含量
K-MANGL
D-甘露糖/D-果糖/D-葡萄糖检测试剂盒
适合测定植物产品和多糖酸性水解产物中D-甘露糖含量
K-MASUG
麦芽糖/蔗糖/D-葡萄糖检测试剂盒
在植物和食品中麦芽糖，蔗糖和葡萄糖的含量检测
K-PECID
胶质识别检测试剂盒
食品配料中果胶的鉴别
K-PHYT
植酸(总磷)检测试剂盒
食品和饲料样品植酸/总磷含量测量的简便方法。不需要通过阴离子交换色谱对植酸纯化，适合于大量样本分析
K-PYRUV
丙酮酸检测试剂盒
在啤酒、葡萄酒、果汁、食品和体液中丙酮酸分析
K-RAFGA
棉子糖/D-半乳糖检测试剂盒
快速测量植物材料和食品中棉子糖和半乳糖含量
K-RAFGL
棉子糖/蔗糖/D-半乳糖检测试剂盒
分析种子和种子粉中D-葡萄糖、蔗糖、棉子糖、水苏糖和毛蕊花糖含量。通过将棉子糖、水苏糖和毛蕊花糖酶解D-葡萄糖、D-果糖和半乳糖，从而测定葡萄糖含量来确定
K-SDAM
淀粉损伤检测试剂盒
谷物面粉中淀粉损伤的检测和分析
K-SUCGL
蔗糖/D-葡萄糖检测试剂盒
饮料、果汁、蜂蜜和食品中蔗糖和葡萄糖的分析
K-SUFRG
蔗糖/D-果糖/D-葡萄糖检测试剂盒
适用于植物和食品中蔗糖、D-葡萄糖和D-果糖的测定
K-TDFR
总膳食纤维检测试剂盒
总膳食纤维检测
K-TREH
海藻糖检测试剂盒
快速、可靠地检测食品、饮料和其它物料中海藻糖含量
K-URAMR
尿素/氨快速检测试剂盒
适用于水、饮料、乳制品和食品中尿素和氨的快速测定
K-URONIC
D-葡萄糖醛酸/D-半乳糖醛酸检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中六元糖醛酸含量（D-葡萄糖醛酸和D-半乳糖醛酸）
K-XYLOSE
D-木糖检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中D-木糖含量
K-YBGL
Beta葡聚糖[酵母和蘑菇]检测试剂盒
检测酵母和蘑菇制品中1,3:1,6-beta-葡聚糖和α-葡聚糖含量
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