Megazyme/&beta-葡糖酶片/T-BGZ-200T/200片
商品编号：
T-BGZ-200T
品牌：
Megazyme INC
市场价：
¥5400.00
美元价：
3240.00
产品分类：
反应底物
公司分类：
Reaction_substrate
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Highpuritydyedandcrosslinkedβ-Glucazymetabletsforthemeasurementofenzymeactivity,forresearch,biochemicalenzymeassaysandinvitrodiagnosticanalysis.
RACIStandardMethod.Fortheassayofmaltβ-glucanaseandendo-cellulase.ContainingAZCL-Barleyβ-glucan.
Novelapproachestotheautomatedassayofβ-glucanaseandlichenaseactivity.
Mangan,D.,Liadova,A.,Ivory,R.&McCleary,B.V.(2016).CarbohydrateResearch,435,162-172.
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Wereporthereinthedevelopmentofanovelassayprocedureforthemeasurementofβ-glucanaseandlichenase(EC3.2.1.73)incrudeenzymeextracts.Twoassayformatsbasedona)adirectcleavageorb)anenzymecoupledsubstratewereinitiallyinvestigated.The‘directcleavage’substrate,namely4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-31-cellotriosyl-β-glucopyranoside(MBG4),wasfoundtobethemoregenerallyapplicablereagent.Thissubstratewasfullycharacterisedusingacrudemaltβ-glucanaseextract,abacteriallichenase(Bacillussp.)andanon-specificendo-1,3(4)-β-glucanasefromClostridiumThermocellum(EC3.2.1.6).Standardcurveswerederivedthatallowtheassayabsorbanceresponsetobedirectlyconvertedtoβ-glucanase/lichenaseactivityonbarleyβ-glucan.ThespecificityofMBG4wasconfirmedbyanalysingtheactionofcompetingglycosylhydrolasesthataretypicallyfoundinmaltonthesubstrate.Manualandautomatedassayformatsweredevelopedfortheanalysisofa)β-glucanaseinmaltflourandb)lichenaseenzymeextractsandtherepeatABIlityoftheseassayswasfullyinvestigated.
Comparisonofendolytichydrolasesthatdepolymerise1,4-β-D-mannan,1,5-α-L-arabinanand1,4-β-D-galactan.
McCleary,B.V.(1991).“EnzymesinBiomassConversion”,(M.E.HimmelandG.F.Leatham,Eds.),ACSSymposiumSeries460,Chapter34,pp.437-449.AmericanChemicalSociety,Washington.
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Hydrolysisofmannan-typepolysaccharidesbyβ-mannanaseisdependentonsubstitutiononandwithinthemain-chainaswellasthesourceoftheβ-mannanaseemployed.Characterisationofreactionproductscanbeusedtodefinethesub-sitebindingrequirementsoftheenzymesaswellasthefine-structuresofthepolysaccharides.Actionofendo-arabinanaseandendo-galactanaseonarabinansandarabinogalactansisdescribed.Specificassaysforendo-arabinanaseandarabinan(infruit-juiceconcentrates)arereported.
Measurementofmaltbeta-glucanase.
McCleary,B.V.(1986).Proceedingsofthe19thConventionoftheInstituteofBrewing(Aust.andN.Z.section),181-187.
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AProcedurehasbeendevelopedfortheassayofmaltβ-glucanase[a(1→3)(1→4)-β-D-glucanase]whichemploysassubstrate,barleyβ-glucandyedwithRemazolbrilliantBlueandchemicallymodifiedwithcarboxymethylgroupstoincreasesolubility.Thedescribedassayproceduretogetherwithamodifiedextractionformatallowsanalysisofuptotenmaltsamplesinlessthan80min.Also,theprocedureisspecificforenzymesactiveonbarleyβ-glucan,isaccurateandreliable,andcanbereADIlyappliedtotheanalysisofβ-glucanaseinmalt,greenmaltandwort.
Asolublechromogenicsubstratefortheassayof(1→3)(1→4)-β-D-glucanase(lichenase).
McCleary,B.V.(1986).CarbohydratePolymers,6(4),307-318.
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Asimpleprocedurefortheassayof(1→3)(1→4)-β-D-glucanase(lichenase)hasbeendeveloped.Thisassayemploysassubstratebarley(1→3)(1→4)-β-D-glucandyedwithRemazolbrilliantBlueRandchemicallymodifiedwithcarboxymethylgroupstoincreasesolubility.Preparationofthissubstraterequiredthedevelopmentofanimprovedprocedurefortheextractionandpurificationofbarleyβ-glucan.AssaysbasedontheuseofthedescribedchromogenicsubstrateatpH6•5aresensitiveandspecificforenzymesactiveonbarleyβ-glucan.
Problemscausedbybarleybeta-glucansinthebrewingindustry.
McCleary,B.V.(1986).ChemistryinAustralia,53,306-308.
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Brewing,theoldestapplicationofbio-technologyisnowamixoftradeartandmodernscience.Thisarticledescribesnewapplicationsofenzymechemistrytotrouble-shootinginbeerproduction.
Assayofmaltβ-glucanaseusingazo-barleyglucan:animprovedprecipitant.
McCleary,B.V.&Shameer,I.(1987).JournaloftheInstituteofBrewing,93(2),87-90.
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Aprocedurerecentlydescribedfortheassayofmaltβ-glucanase,whichemploysadye-labelledandchemically-modifiedbarleyβ-glucansubstrate,hasbeenimprovedbychangingtheprecipitantsolutionusedtoterminatethereaction.Thenewprecipitantsolutioncontains0•4%(w/v)zincacetateand4%(w/v)sodiumacetatedissolvedin80%(v/v)aqueousmethylcellosolve.Withthisprecipitanttheprocedurecanbedirectlyappliedtotheassayofcellulaseactivity,andwithminormodification,totheassayoflichenaseactivity.
Measurementofpolysaccharidedegradingenzymesusingchromogenicandcolorimetricsubstrates.
McCleary,B.V.(1991).ChemistryinAustralia,58,398-401.
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Enzymicdegradationofcarbohydratesisofmajorsignificanceintheindustrialprocessingofcerealsandfruits.Intheproductionofbeer,barleyisgerminatedunderwelldefinedconditions(malting)toinducemaximumenzymesynthesiswithminimumrespirationofreservecarbohydrates.Thegrainsaredriedandthenextractedwithwaterundercontrolledconditions.Theamylolyticenzymessynthesizedduringmalting,aswellasthosepresentintheoriginalbarley,convertthestarchreservestofermentablesugars.Otherenzymesactonthecellwallpolysaccharides,mixed-linkageβ-glucanandarabinoxylan,reducingtheviscosityandthusaidingfiltration,andreducingthepossibilityofsubsequentprecipitationofpolymericmaterial.Inbaking,β-amylaseandα-amylasegivecontrolleddegradationofstarchtofermentablesugarssoastosustainyeastgrowthandgasproduction.Excessquantitiesofα-amylaseintheflourresultinexcessivedegradationofstarchduringbakingwhichinturngivesastickycrumbtextureandsubsequentproblemswithbreadslicing.Juiceyieldfromfruitpulpissignificantlyimprovedifcell-walldegradingenzymesareusedtodestroythethree-dimensionalstructureandwaterbindingcapacityofthepecticpolysaccharidecomponentsofthecellwalls.Problemsofroutineandreliableassayofcarbohydratedegradingenzymesinthepresenceofhighlevelsofsugarcompoundsareexperiencedwithsuchindustrialprocess.
Optimisingtheresponse.
Acamovic,T.&McCleary,B.V.(1996).FeedMix,4,14-19.
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Afinebalanceexistsbetweenenzymeactivityandtheadverseeffectsassociatedwithfeedprocessing.Accurateestimationofenzymeactivityinthefeedisapre-requisitetooptimisingtheresponse.
SecretionofheterologousproteinsinBacillussubtiliscanbeimprovedbyengineeringcellcomponentsaffectingpost‐translocationalproteinfoldinganddegradation.
Vitikainen,M.,Hyyryläinen,H.L.,Kivimäki,A.,Kontinen,V.P.&Sarvas,M.(2005).JournalofAppliedMicroBIOLOGy,99(2),363-375.
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Aims:ToexplorethepotentialtoenhancesecretionofheterologousproteinsinBacillussubtilisbyengineeringcellfactorsaffectingextracytoplasmicproteinfoldinganddegradation.MethodsandResults:Bottleneckcomponentsaffectingtheextracytoplasmicphaseofproteinsecretionweregeneticallyengineeredandtheireffectsonthesecretionof11industriallyinterestingheterologousproteinswerestudiedbyWesternblottingandenzymaticassays.OverproductionofPrsAlipoproteinenhancedthesecretionofα-amylaseofBacillusstearothermophilus(fourfold)andpneumolysin(1•5-fold).IncreasingthenetnegativechargeofthecellwallbecauseoflackoftheD-alaninesubstitutionofanioniccellwallpolymersenhancedthesecretionofpneumolysinc.1•5-fold.DecreasingthelevelofHtrA-typequalitycontrolproteasescausedharmfuleffectsongrowthanddidnotenhancesecretion.Pertussistoxinsubunit,S1wasfoundtobeasubstrateforHtrA-typeproteasesanditssecretionwasdependentontheseproteases.Conclusions:Secretionofheterologousproteinscanbeenhancedbyengineeringcomponentsinvolvedinlatestagesofsecretioninaprotein-dependentmanner.SignificanceandImpactoftheStudy:Thestudyrevealedbothpossibilitiesandlimitationsofmodulatingthepost-translocationalphaseofsecretionasameanstoimprovetheyieldofheterologousproteins.
Lentinulaedodestlg1encodesathaumatin-likeproteinthatisinvolvedinlentinandegradationandfruitingbodysenescence.
Sakamoto,Y.,Watanabe,H.,Nagai,M.,Nakade,K.,Takahashi,M.&Sato,T.(2006).PlantPhysiology,141(2),793-801.
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LentinanisanantitumorproductthatispurifiedfromfreshLentinulaedodesfruitingbodies.Itisacellwallcomponent,comprisingβ-1,3-glucanwithβ-1,6-linkedbranches,whichbecomesdegradedduringpostharvestpreservationasaresultofincreasedglucanaseactivity.Inthisstudy,weusedN-terminalaminoacidsequencetoisolatetlg1,ageneencodingathaumatin-like(TL)proteininL.edodes.TheCDNAclonewasapproximately1.0kbwhereasthegenomicsequencewas2.1kb,andcomparisonofthetwoindicatedthattlg1contains12introns.Thetlg1geneproduct(TLG1)waspredictedtocomprise240aminoacids,withamolecularmassof25kDandisoelectricpointvalueof3.5.Theputativeaminoacidsequenceexhibitsapproximately40%identitywithplantTLproteins,andafungalgenomedatabasesearchrevealedthattheseTLproteinsareconservedinmanyfungiincludingthebasidiomycotaandascomycota.Transcriptionoftlg1wasnotdetectedinvegetativemyceliumoryoungandfreshmushrooms.However,transcriptionincreasedfollowingharvest.Western-blotanalysisdemonstratedariseinTLG1levelsfollowingharvestandsporediffusion.TLG1expressedinEscherichiacoliandAspergillusoryzaeexhibitedβ-1,3-glucanaseactivityand,whenpurifiedfromtheL.edodesfruitingbody,demonstratedlentinandegradingactivity.Thus,wesuggestthatTLG1isinvolvedinlentinanandcellwalldegradationduringsenescencefollowingharvestandsporediffusion.
Thermostabilitiesofgrainβ-amylaseandβ-glucanaseinfinnishlandracebarleysandtheirputativepastadaptedness.
Ahokas,H.&Manninen,M.L.(2000).Hereditas,132(2),111-118.
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Thermostabilityofβ-amylaseactivitywasageneralfeatureinasampleof32Finnishbarleylandraces.OneoftwoFinnishlandracesprobablycontributedthethermostabilitytocv.‘Pirkka’incrossesperformedabout70yearsago.Thestabilityislessevolvedinβ-glucanaseactivityalthoughthemosttoleranttypesappearedinlandracesandinPirkkawithaFinnishlandracebackground.SelectionpressureforthermostabilityingrainsmayhavebeenafeatureoftraditionalcropmanagementpracticesamongFinnsinthepast:dryinggraincrops,includingprematurebarley,aboveanoveninaspecialdryinghouseattemperaturesexceeding55°C,andgerminationinblack,sunlitslash-and-burnsoils,withameasuredsurfacetemperatureof63°C.Apositive,thoughsmallcorrelationbetweenthethermotoleranceratiosofthetwoenzymesmaybearemnantoftheircommonlongselectionpressureendingtensofgenerationspriortocollectioninthe1960sand1970s.
Partialpurificationofcomponentsinryewaterextractableswhichimprovethequalityofoatbread.
Pauly,A.&Delcour,J.A.(2018).JournalofCerealScience,79,141-147.
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Unlikewheatbread,thedoughofwhichhasavisco-elasticnetworkandhighgas-holdingcapacity,oatbreadgenerallyhasalowvolumeandadensestructure.Weshowedearlierthatincludingryewater-extractablecomponentsinanoatbreadbatterrecipeincreasesloafvolumebyca.30%(PaulyandDelcour,submittedasback-to-backpublication).Weherereportoneffortstoidentifytheactivefactor(s).Anionexchangechromatographyallowedenrichingtheactivefactor(s).ThisandthefactthatonlyalimitedvolumeincreasewasobservedwhenoatbatterwassupplementedwithboiledryeextractindicatethatproteinsarelikelythemostimportantcomponentsresponsIBLeforthevolumeincrease.Whilethemostactivefactor(s)hadapIbelow4.5,componentswithpIvaluesbetween4.5and8.5alsocontributedtooatloafvolume.Alkalineryecomponents(pI>8.5)orryearabinoxylanhadnoimpact.Ryewater-extractablecomponentssmallerthan6–8kDaalsohadapositiveimpactonloafvolume.
Impactofwater-extractablecomponentsfromdifferentcerealsonthequalityofoatbread.
Pauly,A.&Delcour,J.A.(2018).JournalofCerealScience,79,134-140.
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Loafvolumeandcrumbstructureofoatbreadarenotcomparabletothoseofbreadfromwheatflour.Hydrocolloids,surfactantsand/orenzymesareoftenincludedinoatbatterrecipesforqualityenhancementreasons.Inthisstudy,weexaminedtheimpactofwater-extractablecomponentsfrombarley,oat,ryeandwheatflouronoatbreadquality.Wespeculatedthatsuchwaterextractscontaincomponentswhichalsowouldenhancethequalityofoatbread.Asexpected,extractprotein,non-starchpolysaccharide,lipidandenzymelevelsvariedwidelyamongstthedifferentcerealfloursused.Theextractsalsovariedinfoamingpropertiesandextractviscosities.Ryeflourcontainedthehighestlevelofwater-extractablecomponents.Inclusionofryeaqueousextractresultedinthelargestloafvolumeincreaseandinsoftercrumbthannotedforcontroloatbread.Rheofermentometeranalysesshowedthatthemomentofgascellopeningwasdelayedwhenryeextractwasadded,indicatingimprovedbattergascellstabilization,whilecollapseduringbakingwasnotaffected.Theoatbreadimprovingeffectoftheryeextractislikelyduetoacombinationoftheimpactofdifferentofitsconstituentssuchasenzymesandsurfaceactivecomponents.
品牌介绍
Megazyme品牌产品简介
来源：作者：人气：2149发表时间：2016-05-19 10:59:00【大 中 小】
Megazyme是一家全球性公司，专注于开发和提供用于饮料、谷物、乳制品、食品、饲料、发酵、生物燃料和葡萄酒产业用的分析试剂、酶和检测试剂盒。Megazyme的许多检测试剂盒产品已经为众多官方科学协会（包括AOAC, AACC , RACI, EBC和ICC等），经过严格的审核，批准认证为官方标准方法，确保以准确、可靠、定量和易于使用的测试方法，满足客户的质量诉求。
Megazyme的主要产品线包括：
◆ 检测试剂盒
◆ 酶
◆ 酶底物
◆ 碳水化合物
◆ 化学品/仪器
官网地址：http://www.megazyme.com
检测试剂盒特色产品：
货号
中文品名
用途
K-ACETAF
乙酸[AF法]检测试剂盒
酶法定量分析乙酸最广泛使用的方法
K-ACHDF
可吸收糖/膳食纤维检测试剂盒
酒精沉淀法测定膳食纤维
K-AMIAR
氨快速检测试剂盒
用于包括葡萄汁、葡萄酒以及其它食品饮料样品中氨含量的快速检测分析。
K-AMYL
直链淀粉/支链淀粉检测试剂盒
谷物淀粉和而粉中直链淀粉/支链淀粉比例和含量检测
K-ARAB
阿拉伯聚糖检测试剂盒
果汁浓缩液中阿拉伯聚糖的检测
K-ASNAM
L-天冬酰胺/L-谷氨酰胺和氨快速检测试剂盒
用于食品工业中丙烯酰胺前体、细胞培养基、以及上清液组分中、L-天冬酰胺，谷氨酰胺和氨的检测分析
K-ASPTM
阿斯巴甜检测试剂盒
专业用于测定饮料和食品中阿斯巴甜含量，操作简单
K-BETA3
β-淀粉酶检测试剂盒
适用于麦芽粉中β-淀粉酶的测定
K-BGLU
混合键β-葡聚糖检测试剂盒
测定谷物、荞麦粉、麦汁、啤酒及其它食品中混合键β-葡聚糖(1,3:1,4-β-D-葡聚糖)的含量
K-CERA
α-淀粉酶检测试剂盒
谷物和发酵液（真菌和细菌）中α-淀粉酶的分析测定
K-CITR
柠檬酸检测试剂盒
快速、可靠地检测食品、饮料和其它物料中柠檬酸（柠檬酸盐）含量
K-DLATE
乳酸快速检测试剂盒
快速、特异性检测饮料、肉类、奶制品和其它食品中L-乳酸和D-乳酸(乳酸盐）含量
K-EBHLG
酵母β-葡聚糖酶检测试剂盒
用于测量和分析酵母中1,3:1,6?-β-葡聚糖，也可以检测1,3-葡聚糖
K-ETSULPH
总亚硫酸检测试剂盒
测定葡萄酒、饮料、食品和其他物料中总亚硫酸含量（按二氧化硫计）的一种简单，高效，可靠的酶法检测方法
K-FRGLMQ
D-果糖/D-葡萄糖[MegaQuant法]检测试剂盒
适用于使用megaquant?色度计（505nm下）测定葡萄、葡萄汁和葡萄酒中D-果糖和D-葡萄糖的含量。
K-FRUC
果聚糖检测试剂盒
含有淀粉、蔗糖和其他糖类的植物提取物和食品中果聚糖的含量测定。
K-FRUGL
D-果糖/D-葡萄糖检测试剂盒
对植物和食品中果糖或葡萄糖含量的酶法紫外分光测定。
K-GALM
半乳甘露聚糖检测试剂盒
食品和植物产品中半乳甘露聚糖的含量检测
K-GLUC
D-葡萄糖[GOPOD]检测试剂盒
谷物提取物中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUHK
D-葡萄糖[HK]检测试剂盒
植物和食品中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUM
葡甘聚糖检测试剂盒
植物和食品中葡甘聚糖的含量测定。
K-INTDF
总膳食纤维检测试剂盒
总膳食纤维特定检测和分析
K-LACGAR
乳糖/D-半乳糖快速检测试剂盒
用于快速检测食品和植物产品中乳糖、D-半乳糖和L-阿拉伯糖
K-LACSU
乳糖/蔗糖/D-葡萄糖检测试剂盒
混合面粉和其它物料中蔗糖、乳糖和D-葡萄糖的测定
K-LACTUL
乳果糖检测试剂盒
特异性、快速和灵敏测量奶基样品中乳果糖含量
K-MANGL
D-甘露糖/D-果糖/D-葡萄糖检测试剂盒
适合测定植物产品和多糖酸性水解产物中D-甘露糖含量
K-MASUG
麦芽糖/蔗糖/D-葡萄糖检测试剂盒
在植物和食品中麦芽糖，蔗糖和葡萄糖的含量检测
K-PECID
胶质识别检测试剂盒
食品配料中果胶的鉴别
K-PHYT
植酸(总磷)检测试剂盒
食品和饲料样品植酸/总磷含量测量的简便方法。不需要通过阴离子交换色谱对植酸纯化，适合于大量样本分析
K-PYRUV
丙酮酸检测试剂盒
在啤酒、葡萄酒、果汁、食品和体液中丙酮酸分析
K-RAFGA
棉子糖/D-半乳糖检测试剂盒
快速测量植物材料和食品中棉子糖和半乳糖含量
K-RAFGL
棉子糖/蔗糖/D-半乳糖检测试剂盒
分析种子和种子粉中D-葡萄糖、蔗糖、棉子糖、水苏糖和毛蕊花糖含量。通过将棉子糖、水苏糖和毛蕊花糖酶解D-葡萄糖、D-果糖和半乳糖，从而测定葡萄糖含量来确定
K-SDAM
淀粉损伤检测试剂盒
谷物面粉中淀粉损伤的检测和分析
K-SUCGL
蔗糖/D-葡萄糖检测试剂盒
饮料、果汁、蜂蜜和食品中蔗糖和葡萄糖的分析
K-SUFRG
蔗糖/D-果糖/D-葡萄糖检测试剂盒
适用于植物和食品中蔗糖、D-葡萄糖和D-果糖的测定
K-TDFR
总膳食纤维检测试剂盒
总膳食纤维检测
K-TREH
海藻糖检测试剂盒
快速、可靠地检测食品、饮料和其它物料中海藻糖含量
K-URAMR
尿素/氨快速检测试剂盒
适用于水、饮料、乳制品和食品中尿素和氨的快速测定
K-URONIC
D-葡萄糖醛酸/D-半乳糖醛酸检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中六元糖醛酸含量（D-葡萄糖醛酸和D-半乳糖醛酸）
K-XYLOSE
D-木糖检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中D-木糖含量
K-YBGL
Beta葡聚糖[酵母和蘑菇]检测试剂盒
检测酵母和蘑菇制品中1,3:1,6-beta-葡聚糖和α-葡聚糖含量
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