Megazyme/Red Debranched Arabinan（甜菜）/S-RDAR/2克
商品编号：
S-RDAR
品牌：
Megazyme INC
市场价：
¥3288.00
美元价：
1972.80
产品分类：
反应底物
公司分类：
Reaction_substrate
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Highpuritydyed,solubleRedDebranchedArABInan(SugarBeet)forthemeasurementofenzymeactivity,forresearch,biochemicalenzymeassaysandinvitrodiagnosticanalysis.
DebranchedarabinandyedwithProcionReddye.Substratefortheassayofendo-1,5-α-L-arabinanase.
PurificationandcharacterizationofanovelThermostableα-L-arabinofuranosidasefromacolor-variantstrainofAureobasidiumpullulans.
Saha,B.C.&Bothast,R.J.(1998).AppliedandEnvironmentalMicroBIOLOGy,64(1),216-220.
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Acolor-variantstrainofAureobasidiumpullulans(NRRLY-12974)producedα-L-arabinofuranosidase(α-L-AFase)whengrowninliquidcultureonoatspeltxylan.Anextracellularα-L-AFasewaspurified215-foldtohomogeneityfromtheculturesupernatantbyammoniumsulfatetreatment,DEAEBio-GelAagarosecolumnchromatography,gelfiltrationonaBio-GelA-0.5mcolumn,arabinan-Sepharose6Baffinitychromatography,andSP-SephadexC-50columnchromatography.Thepurifiedenzymehadanativemolecularweightof210,000andwascomposedoftwoequalsubunits.Ithadahalf-lifeof8hat75°C,displayedoptimalactivityat75°CandpH4.0to4.5,andhadaspecificactivityof21.48µmolmin-1·mg-1·ofproteinagainstp-nitrophenyl-α-L-arabinofuranoside(pNPαAF).Thepurifiedα-L-AFasereADIlyhydrolyzedarabinananddebranchedarabinanandreleasedarabinosefromarabinoxylansbutwasinactiveagainstarabinogalactan.TheKmvaluesoftheenzymeforthehydrolysisofpNPαAF,arabinan,anddebranchedarabinanat75°CandpH4.5were0.26mM,2.14mg/ml,and3.25mg/ml,respectively.Theα-L-AFaseactivitywasnotinhibitedatallbyL-arabinose(1.2M).Theenzymedidnotrequireametalionforactivity,anditsactivitywasnotaffectedbyp-chloromercuribenzoate(0.2mM),EDTA(10mM),ordithiothreitol(10mM).
DirectinterferencewithrhamnogalacturonanIbiosynthesisinGolgivesicles.
Skjøt,M.,Pauly,M.,Bush,M.S.,Borkhardt,B.,McCann,M.C.&Ulvskov,P.(2002).PlantPhysiology,129(1),95-102.
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Pectinisaclassofcomplexcellwallpolysaccharideswithmultiplerolesduringcelldevelopment.Assigningspecificfunctionstoparticularpolysaccharidesisinitsinfancy,inpart,becauseofthelimitednumberofmutantsandtransformantsavailablewithmodifiedpecticpolymersintheirwalls.Pectinsarealsoimportantpolymerswithdiverseapplicationsinthefoodandpharmaceuticalindustries,whichwouldbenefitfromtechnologyforproducingpectinswithspecificfunctionalproperties.Inthisreport,wedescribethegenerationofpotato(SolanumtuberosumL.cvPosmo)tubertransformantsproducingpecticrhamnogalacturonanI(RGI)withalowlevelofarabinosylation.ThiswasachievedbytheexpressionofaGolgimembrane-anchoredendo-α-1,5-arabinanase.SugarcompositionanalysisofRGIisolatedfromtransformedandwild-typetubersshowedthatthearabinosecontentwasdecreasedbyapproximately70%intransformedcellwallscomparedwithwildtype.ThemodificationoftheRGIwasconfirmedbyimmunolabelingwithanantibodyrecognizingα-1,5-arabinan.Thisisthefirsttime,toourknowledge,thatthebiosynthesisofaplantcellwallpolysaccharidehasbeenmanipulatedthroughtheactionofaglycosylhydrolasetargetedtotheGolgicompartment.
Effectofcarbonsourceonproductionofα-L-arabinofuranosidasebyAureobasidiumpullulans.
Saha,B.C.&Bothast,R.J.(1998).CurrentMicrobiology,37(5),337-340.
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Acolor-variantstrainofAureobasidiumpullulans(NRRLY-12974)producedα-L-arabinofuranosidase(α-L-AFase)whengrowninliquidcultureonsugarbeetarabinan,wheatarabinoxylan,L-arabinose,L-arabitol,xylose,xylitol,oatspeltxylan,cornfiber,orarabinogalactan.L-Arabinosewasmosteffectiveforproductionofbothwhole-brothandextracellularα-L-AFaseactivity,followedbyL-arabitol.Oatspeltxylan,sugarbeetarabinan,xylose,xylitol,andwheatarabinoxylanwereintermediateintheirabilitytosupportα-L-AFaseproduction.Loweramountsofenzymeactivityweredetectedincornfiber-andarabinogalactan-growncultures.
Characterizationofabn2(yxiA),encodingaBacillussubtilisGH43arabinanase,Abn2,anditsroleinarabino-polysaccharidedegradation.
Inácio,J.M.&DeSá-Nogueira,I.(2008).JournalofBacteriology,190(12),4272-4280.
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Theextracellulardepolymerizationofarabinopolysaccharidesbymicroorganismsisaccomplishedbyarabinanases,xylanases,andgalactanases.Here,wecharacterizeanovelendo-α-1,5-L-arabinanase(EC3.2.1.99)fromBacillussubtilis,encodedbytheyxiAgene(hereinrenamedabn2)thatcontributestoarabinandegradation.FunctionalstudiesbymutationalanalysisshowedthatAbn2,togetherwithpreviouslycharacterizedAbnA,isresponsIBLeforthemajorityoftheextracellulararabinanactivityinB.subtilis.Abn2wasoverproducedinEscherichiacoli,purifiedfromtheperiplasmicfraction,andcharacterizedwithrespecttosubstratespecificityandbiochemicalandphysicalproperties.Withlinear-α-1,5-L-arabinanasthepreferredsubstrate,theenzymeexhibitedanapparentKmof2.0mgml-1andVmaxof0.25mmolmin-1·mg-1·atpH7.0and50°C.RNAstudiesrevealedthemonocistronicnatureofabn2.Twopotentialtranscriptionalstartsiteswereidentifiedbyprimerextensionanalysis,andbothaσA--dependentandaσH-dependentpromoterwerelocated.Transcriptionalfusionstudiesrevealedthattheexpressionofabn2isstimulatedbyarabinanandpectinandrepressedbyglucose;however,arabinoseisnotthenaturalinducer.Additionally,trans-actingfactorsandciselementsinvolvedintranscriptionwereinvestigated.Abn2displayedacontrolmechanismatalevelofgeneexpressiondifferentfromthatobservedwithAbnA.Thesedistinctregulatorymechanismsexhibitedbytwomembersofextracellularglycosidehydrolasefamily43(GH43)suggestanadaptativestrategyofB.subtilisforoptimaldegradationofarabinopolysaccharides.
Cloning,purification,andcharacterizationofathermostableα-L-arabinofuranosidasefromAnoxybacilluskestanbolensisAC26Sari.
Canakci,S.,Kacagan,M.,Inan,K.,Belduz,A.O.&Saha,B.C.(2008).AppliedMicrobiologyandBiotechnology,81(1),61-68.
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Thegene,AbfAC26Sari,encodinganα-L-arabinofuranosidasefromAnoxybacilluskestanbolensisAC26Sari,wasisolated,cloned,sequenced,andcharacterizated.Onthebasisofaminoacidsequencesimilarities,this57-kDaenzymecouldbeassignedtofamily51oftheglycosylhydrolaseclassificationsystem.Characterizationofthepurifiedrecombinantα-L-arabinofuranosidaseproducedinEscherichiacoliBL21revealedthatitisactiveatabroadpHrange(pH4.5to9.0)andatabroadtemperaturerange(45–85°C)andithasanoptimumpHof5.5andanoptimumtemperatureof65°C.Kineticexperimentat65°Cwithp-nitrophenylα-L-arabinofuranosideasasubstrategaveaVmaxandKmvaluesof1,019U/mgand0.139mM,respectively.Theenzymehadnoapparentrequirementofmetalionsforactivity,anditsactivitywasstronglyinhibitedby1mMCu+2andHg+2.TherecombinantarabinofuranosidasereleasedL-arabinosefromarabinan,arabinoxylan,oatspeltxylan,arabinobiose,arabinotriose,arabinotetraose,andarabinopentaose.Endoarabinanaseactivitywasnotdetected.ThesefindingssuggestthatAbfAC26Sariisanexo-actingenzyme.
Purificationandfunctionalcharacterizationofanovelα-L-arabinofuranosidasefromBifidobacteriumlongumB667.
Margolles,A.&DelosReyes-Gavilan,C.G.(2003).AppliedandEnvironmentalMicrobiology,69(9),5096-5103.
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Thegeneencodinganovelα-L-arabinofuranosidasefromBifidobacteriumlongumB667,abfB,wasclonedandsequenced.Thededucedproteinhadamolecularmassofabout61kDa,andanalysisofitsaminoacidsequencerevealedsignificanthomologyandconservationofdifferentcatalyticresidueswithα-L-arabinofuranosidasesbelongingtofamily51oftheglycosidehydrolases.Regionsflankingthegenecomprisedtwodivergentlytranscribedopenreadingframescodingforhypotheticalproteinsinvolvedinsugarmetabolism.AhistidinetagwasintroducedattheCterminusofAbfB,andtherecombinantproteinwasoverexpressedinLactococcuslactisundercontrolofthetightlyregulated,nisin-induciblenisApromoter.Theenzymewaspurifiedbynickelaffinitychromatography.Themolecularmassofthenativeprotein,asdeterminedbygelfiltration,wasabout260kDa,suggestingahomotetramericstructure.AbfBwasactiveatabroadpHrange(pH4.5to7.5)andatabroadtemperaturerange(20to70°C),andithadanoptimumpHof6.0andanoptimumtemperatureof45°C.Theenzymeseemedtobelessthermostablethanmostpreviouslydescribedarabinofuranosidasesandhadahalf-lifeofabout3hat55°C.Chelatingandreducingagentsdidnothaveanyeffectonitsactivity,butthepresenceofCu2+,Hg2+,andZn2+markedlyreducedenzymaticactivity.Theproteinexhibitedahighlevelofactivitywithp-nitrophenylα-L-arabinofuranoside,withapparentKmandVmaxvaluesof0.295mMand417U/mg,respectively.AbfBreleasedL-arabinosefromarabinan,arabinoxylan,arabinobiose,arabinotriose,arabinotetraose,andarabinopentaose.Noendoarabinanaseactivitywasdetected.ThesefindingssuggestthatAbfBisanexo-actingenzymeandmayplayarole,togetherwithotherglycosidases,inthedegradationofL-arabinose-containingpolysaccharides.
Purification,characterizationandfunctionalanalysisofanendo‐arabinanase(AbnA)fromBacillussubtilis.
Leal,T.F.&DeSá‐Nogueira,I.(2004).FEMSMicrobiologyLetters,241(1),41-48.
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BacillussubtilissynthesizesatleastonearabinanaseencodedbytheabnAgenethatisabletodegradethepolysaccharidearabinan.Here,wereporttheexpressioninEscherichiacoliofthefull-lengthabnAcodingregionwithaHis6-tagfusedtotheC-terminus.TherecombinantproteinwassecretedtotheperiplasmicspaceandcorrectlyprocessedbytheE.colisignalpeptidase.ThesubstratespecificityofpurifiedAbnA,thephysico-chemicalpropertiesandkineticparametersweredetermined.FunctionalanalysisstudiesrevealedGlu215asakeyresidueforAbnAhydrolyticactivityandindicatedthatinadditiontoAbnAB.subtilissecretesotherenzyme(s)abletodegradelinear1,5-α-L-arabinan.
品牌介绍
Megazyme品牌产品简介
来源：作者：人气：2149发表时间：2016-05-19 10:59:00【大 中 小】
Megazyme是一家全球性公司，专注于开发和提供用于饮料、谷物、乳制品、食品、饲料、发酵、生物燃料和葡萄酒产业用的分析试剂、酶和检测试剂盒。Megazyme的许多检测试剂盒产品已经为众多官方科学协会（包括AOAC, AACC , RACI, EBC和ICC等），经过严格的审核，批准认证为官方标准方法，确保以准确、可靠、定量和易于使用的测试方法，满足客户的质量诉求。
Megazyme的主要产品线包括：
◆ 检测试剂盒
◆ 酶
◆ 酶底物
◆ 碳水化合物
◆ 化学品/仪器
官网地址：http://www.megazyme.com
检测试剂盒特色产品：
货号
中文品名
用途
K-ACETAF
乙酸[AF法]检测试剂盒
酶法定量分析乙酸最广泛使用的方法
K-ACHDF
可吸收糖/膳食纤维检测试剂盒
酒精沉淀法测定膳食纤维
K-AMIAR
氨快速检测试剂盒
用于包括葡萄汁、葡萄酒以及其它食品饮料样品中氨含量的快速检测分析。
K-AMYL
直链淀粉/支链淀粉检测试剂盒
谷物淀粉和而粉中直链淀粉/支链淀粉比例和含量检测
K-ARAB
阿拉伯聚糖检测试剂盒
果汁浓缩液中阿拉伯聚糖的检测
K-ASNAM
L-天冬酰胺/L-谷氨酰胺和氨快速检测试剂盒
用于食品工业中丙烯酰胺前体、细胞培养基、以及上清液组分中、L-天冬酰胺，谷氨酰胺和氨的检测分析
K-ASPTM
阿斯巴甜检测试剂盒
专业用于测定饮料和食品中阿斯巴甜含量，操作简单
K-BETA3
β-淀粉酶检测试剂盒
适用于麦芽粉中β-淀粉酶的测定
K-BGLU
混合键β-葡聚糖检测试剂盒
测定谷物、荞麦粉、麦汁、啤酒及其它食品中混合键β-葡聚糖(1,3:1,4-β-D-葡聚糖)的含量
K-CERA
α-淀粉酶检测试剂盒
谷物和发酵液（真菌和细菌）中α-淀粉酶的分析测定
K-CITR
柠檬酸检测试剂盒
快速、可靠地检测食品、饮料和其它物料中柠檬酸（柠檬酸盐）含量
K-DLATE
乳酸快速检测试剂盒
快速、特异性检测饮料、肉类、奶制品和其它食品中L-乳酸和D-乳酸(乳酸盐）含量
K-EBHLG
酵母β-葡聚糖酶检测试剂盒
用于测量和分析酵母中1,3:1,6?-β-葡聚糖，也可以检测1,3-葡聚糖
K-ETSULPH
总亚硫酸检测试剂盒
测定葡萄酒、饮料、食品和其他物料中总亚硫酸含量（按二氧化硫计）的一种简单，高效，可靠的酶法检测方法
K-FRGLMQ
D-果糖/D-葡萄糖[MegaQuant法]检测试剂盒
适用于使用megaquant?色度计（505nm下）测定葡萄、葡萄汁和葡萄酒中D-果糖和D-葡萄糖的含量。
K-FRUC
果聚糖检测试剂盒
含有淀粉、蔗糖和其他糖类的植物提取物和食品中果聚糖的含量测定。
K-FRUGL
D-果糖/D-葡萄糖检测试剂盒
对植物和食品中果糖或葡萄糖含量的酶法紫外分光测定。
K-GALM
半乳甘露聚糖检测试剂盒
食品和植物产品中半乳甘露聚糖的含量检测
K-GLUC
D-葡萄糖[GOPOD]检测试剂盒
谷物提取物中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUHK
D-葡萄糖[HK]检测试剂盒
植物和食品中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUM
葡甘聚糖检测试剂盒
植物和食品中葡甘聚糖的含量测定。
K-INTDF
总膳食纤维检测试剂盒
总膳食纤维特定检测和分析
K-LACGAR
乳糖/D-半乳糖快速检测试剂盒
用于快速检测食品和植物产品中乳糖、D-半乳糖和L-阿拉伯糖
K-LACSU
乳糖/蔗糖/D-葡萄糖检测试剂盒
混合面粉和其它物料中蔗糖、乳糖和D-葡萄糖的测定
K-LACTUL
乳果糖检测试剂盒
特异性、快速和灵敏测量奶基样品中乳果糖含量
K-MANGL
D-甘露糖/D-果糖/D-葡萄糖检测试剂盒
适合测定植物产品和多糖酸性水解产物中D-甘露糖含量
K-MASUG
麦芽糖/蔗糖/D-葡萄糖检测试剂盒
在植物和食品中麦芽糖，蔗糖和葡萄糖的含量检测
K-PECID
胶质识别检测试剂盒
食品配料中果胶的鉴别
K-PHYT
植酸(总磷)检测试剂盒
食品和饲料样品植酸/总磷含量测量的简便方法。不需要通过阴离子交换色谱对植酸纯化，适合于大量样本分析
K-PYRUV
丙酮酸检测试剂盒
在啤酒、葡萄酒、果汁、食品和体液中丙酮酸分析
K-RAFGA
棉子糖/D-半乳糖检测试剂盒
快速测量植物材料和食品中棉子糖和半乳糖含量
K-RAFGL
棉子糖/蔗糖/D-半乳糖检测试剂盒
分析种子和种子粉中D-葡萄糖、蔗糖、棉子糖、水苏糖和毛蕊花糖含量。通过将棉子糖、水苏糖和毛蕊花糖酶解D-葡萄糖、D-果糖和半乳糖，从而测定葡萄糖含量来确定
K-SDAM
淀粉损伤检测试剂盒
谷物面粉中淀粉损伤的检测和分析
K-SUCGL
蔗糖/D-葡萄糖检测试剂盒
饮料、果汁、蜂蜜和食品中蔗糖和葡萄糖的分析
K-SUFRG
蔗糖/D-果糖/D-葡萄糖检测试剂盒
适用于植物和食品中蔗糖、D-葡萄糖和D-果糖的测定
K-TDFR
总膳食纤维检测试剂盒
总膳食纤维检测
K-TREH
海藻糖检测试剂盒
快速、可靠地检测食品、饮料和其它物料中海藻糖含量
K-URAMR
尿素/氨快速检测试剂盒
适用于水、饮料、乳制品和食品中尿素和氨的快速测定
K-URONIC
D-葡萄糖醛酸/D-半乳糖醛酸检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中六元糖醛酸含量（D-葡萄糖醛酸和D-半乳糖醛酸）
K-XYLOSE
D-木糖检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中D-木糖含量
K-YBGL
Beta葡聚糖[酵母和蘑菇]检测试剂盒
检测酵母和蘑菇制品中1,3:1,6-beta-葡聚糖和α-葡聚糖含量
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