Megazyme/偶氮角豆半乳甘露聚糖/S-ACGLM/4克
商品编号:
S-ACGLM
品牌:
Megazyme INC
市场价:
¥3864.00
美元价:
2318.40
产品分类:
反应底物
公司分类:
Reaction_substrate
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Highpuritydyed,solubleAzo-CarobGalactomannanforthemeasurementofenzymeactivity,forresearch,biochemicalenzymeassaysandinvitrodiagnosticanalysis.
Substrateforthespecificmeasurementofendo-1,4-β-D-mannanase.
Asimpleassayprocedureforβ-D-mannanase.
McCleary,B.V.(1978).CarbohydrateResearch,67(1),213-221.
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Asimpleassayprocedureforβ-D-mannanaseenzymehasbeendevelopedwhichemployscarobD-galacto-D-mannandyedwithRemazolbrilliantBlue.Additionally,theprocedureisquantitative,relativelysensitive,andhighlyspecificforβ-D-mannanaseenzyme.ItcanbereADIlyusedforthedeterminationofβ-D-mannanaseactivityincrudeenzymepreparationsandcolumn-chromatographyeluates.
Regulationofendo-actingglycosylhydrolasesinthehyperThermophilicbacteriumThermotogamaritimagrownonglucan-andmannan-basedpolysaccharides.
Chhabra,S.R.,Shockley,K.R.,Ward,D.E.&Kelly,R.M.(2002).AppliedandEnvironmentalMicroBIOLOGy,68(2),545-554.
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ThegenomesequenceofthehyperthermophilicbacteriumThermotogamaritimaencodesanumberofglycosylhydrolases.Manyoftheseenzymeshavebeenshowninvitrotodegradespecificglycosidesthatpresumablyserveascarbonandenergysourcesfortheorganism.However,becauseofthebroadsubstratespecificityofmanyglycosylhydrolases,itisdifficulttodeterminethephysiologicalsubstratepreferencesforspecificenzymesfrombiochemicalinformation.Inthisstudy,T.maritimawasgrownonarangeofpolysaccharides,includingbarleyβ-glucan,carboxymethylcellulose,carobgalactomannan,konjacglucomannan,andpotatostarch.
Effectofenzymetreatmentonchemicalcompositionandproductionofreducingsugarsinpalm(Elaeisguineenis)kernelexpeller.
Saenphoom,P.,Liang,J.B.,Ho,Y.W.,Loh,T.C.&Rosfarizan,M.(2011).AfricanJournalofBiotechnology,10(68),15372-15377,
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Theprimaryobjectiveofthisstudywastoexaminetheeffectsofenzymetreatmentonchemicalcompositionsandproductionofreducingsugarsinpalm(Elaeisguineenis)kernelexpeller(PKE).PKEwasincubatedin80%watercontentat55°Cfor18hwithenzymeorwithoutenzyme(ascontrol).Crudeprotein(CP)andgrossenergy(GE)contentsofPKEwerenotsignificantlydifferent(P>0.05)betweenthecontrolandenzymetreatedPKE;averaged17.95%forCPand15.88MJ/kgforGE.However,etherextract(EE)neutraldetergentfiber(NDF),aciddetergentfiber(ADF),hemicelluloseandcellulosecontentsofenzymetreatedPKEdecreased(P<0.01) by="" approximately="" 34.6,="" 26,="" 20,="" 35.7="" and="" 22.1%,="" respectively,="" compared="" with="" the="" control.="" total="" content="" of="" reducing="" sugars="" (mannose,="" glucose="" and="" galactose)="" of="" the="" enzyme="" treated="" pke="" increased="" by="" approximately="" 200="" folds="" compared="" to="" the="" control="">0.01)><0.01). enzyme="" treated="" pke="" had="" higher="">0.01).><0.01) cellulase="" and="" mannanase="" activities="" but="" not="" α-galactosidase.="">0.01)>
Indirectmethodforquantificationofcellbiomassduringsolid-statefermentationofpalmkernelcakebasedonproteincontent.
ABD-Aziz,S.,Hung,G.S.,Hassan,M.A.,Karim,M.I.A.&Samat,N.(2008).AsianJournalofScientificResearch,1(4),385-393.
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Solid-StateFermentation(SSF)ofAspergillusnigerFTCC5003onPalmKernelCake(PKC)isapracticalapproachtoupgradePKCintovalueaddedproduct.PresentstudywasconductedonAspergillusnigerFTCC5003growthprofileandmodelsthatareabletodescribethegrowthinSSFusingPKCsubstrate.DuetothedifficultiesofseparatingcellbiomassquantitativelyfromthesubstrateforSSFsystems,indirectmethodformeasurementofcellgrowthduringSSFofPKCbyAspergillusnigerFTCC5003wasstudiedbasedontheestimationofglucosamineandproteincontent.Preliminaryrelationshipsbetweenglucosamineandproteincontentstofungaldrycellweight(Dw)weredevelopedusingsimulatedhomogenousSSFdatausingglassbeadsassupportmaterials.BothglucosamineandproteincontentswerewellcorrelatedtothefungaldrycellweightinSSFonsupportmaterialsforproteinandglucosamine,respectively.TheequationsobtainedwereusedfortheestimationofcellbiomassprofileduringSSFofPKCfromthedataofglucosamineandproteinasgrowthindicatorstudy.Theestimatedfungaldrycellweightbasedonproteinconcentrationandβ-mannanaseactivityasmetabolicactivityformicrobialgrowthwerewellcorrelatedtoPKCdryweightwhich,indicatingthatbothweresuitableMarkerindescribingthegrowthofA.nigerFTCC5003inthissystem.Incontrast,estimatedfungaldrycellweightbasedonglucosamineconcentrationwasnotsuitabletodescribethegrowthofA.nigerFTCC5003.
Isolation,screeningandidentificationofmannanaseproducermicroorganisms(Pemencilan,penyaringandanpengenalpastianmikroorganismapenghasilenzimmannanase).
Asfamawi,K.K.,Noraini,S.&Darah,I.(2013).JournalofTropicalAgricultureandFoodScience,41(1),169–177.
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Palmkernelcake(PKC)isusedwidelyintheanimalfeedindustrybuthaslimiteduseinpoultryfeedduetoitshighfibreandlowproteincontents.Themajorcomponentofthefibreismannanwhichisinsolubleanddifficulttodigest.Therefore,isolationoflocalpotentialmicrobescapableofdegradingmannanwasexploredfromvarioussourcessuchaspeatsoil,rottenoilpalmtrunksandrawPKC.TheisolateswerescreenedbasedontheclearingzonemethodonselectiveagarmediacontainingAzo-carob-galactomannanassubstrate.Atotalof36isolateswerescreenedandonly13hadaclearzonerangingfrom3.42±0.02mmto5.44±0.06mmwhichwereusedforfurtheranalysis.Theseisolateswereincubatedinshakeflasksat35°Cfor48hwith10g/litrePKCassubstrate.ThebestenzymesproducerwasisolateIBRLF16.A4withspecificmannanaseenzymeacitivityof17.82±0.05U/mg,productionofglucosamineat9.84±0.11mg/gandmannoseat9.54±0.06mg/g.TheisolateIBRLF16.A4wasidentifiedasAspergillusnigerIBRLF16.A4usingascanningelectronmicroscope.
Afamily26mannanaseproducedbyClostridiumthermocellumasacomponentofthecellulosomecontainsadomainwhichisconservedinmannanasesfromanaerobicfungi.
Halstead,J.R.,Vercoe,P.E.,Gilbert,H.J.,Davidson,K.&Hazlewood,G.P.(1999).Microbiology,145,3101–3108.
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CellulosomespreparedbythecelluloseaffinitydigestionmethodfromClostridiumthermocellumculturesupernatanthydrolysedcarobgalactomannanduringincubationat60°CandpH6.5.ArecombinantphageexpressingmannanaseactivitywasisolatedfromalibraryofC.thermocellumgenomicDNAconstructedinλZAPII.TheclonedfragmentofDNAcontainingaputativemannanasegene(manA)wassequenced,revealinganORFof1767nt,encodingaprotein(mannanaseA;Man26A)of589aawithamolecularmassof66816Da.Theputativecatalyticdomain(CD)ofMan26A,identifiedbygenesectioningandsequencecomparisons,displayedupto32%identitywithothermannanasesbelongingtofamily26.ImmediatelydownstreamoftheCDandseparatedfromitbyashortproline/threoninelinkerwasaduplicated24-residuedockerinmotif,whichisconservedinallC.thermocellumcellulosomalenzymesdescribedthusfarandmediatestheirattachmenttothecellulosome-integratingprotein(CipA).Man26AconsistingoftheCDalone(Man26A")washyperexpressedinEscherichiacoliBL21(DE3)andpurified.Thetruncatedenzymehydrolysedsolubleandinsolublemannan,displayingatemperatureoptimumof65°CandapHoptimumof6.5,butexhibitednoactivityagainstotherplantcellwallpolysaccharides.AntiserumraisedagainstMan26A’cross-reactedwithapolypeptidewithamolecularmassof70000DathatispartoftheC.thermocellumcellulosome.AsecondvariantofMan26AcontainingtheN-terminalsegmentof130residuesandtheCD(Man26A")boundtoivory-nutmannanandweaklytosolubleCarobgalactomannanandinsolublecellulose.Man26A"consistingoftheCDalonedidnotbindtothesepolysaccharides.TheseresultsindicatethattheN-terminal130residuesofmatureMan26Amayconstituteaweakmannan-bindingdomain.SequencecomparisonsrevealedalackofidentitybetweenthisregionofMan26Aandotherpolysaccharide-bindingdomains,butsignificantidentitywitharegionconservedinthethreefamily26mannanasesfromtheanaerobicfungusPiromycesequi.
Characterizationofsalt-adaptedsecretedlignocellulolyticenzymesfromthemangrovefungusPestalotiopsissp.
Arfi,Y.,Chevret,D.,Henrissat,B.,Berrin,J.G.,Levasseur,A.&Record,E.(2013).NatureCommunications,4,ArticleNumber1810.
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Fungiareimportantforbiomassdegradationprocessesinmangroveforests.Giventhepresenceofseawaterintheseecosystems,mangrovefungiareadaptedtohighsalinity.HereweisolatePestalotiopsissp.NCi6,ahalotolerantandlignocellulolyticmangrovefungusoftheorderXylariales.Westudyitslignocellulolyticenzymesandanalysetheeffectsofsalinityonitssecretomes.Denovotranscriptomesequencingandassemblyindicatethatthisfunguspossessesofover400putativelignocellulolyticenzymes,includingalargefractioninvolvedinlignindegradation.Proteomicanalysesofthesecretomessuggestthatthepresenceofsaltmodifieslignocellulolyticenzymecomposition,withanincreaseinthesecretionofxylanasesandcellulasesandadecreaseintheproductionofoxidases.Asaresult,celluloseandhemicellulosehydrolysisisenhancedbutligninbreakdownisreduced.Thisstudyhighlightstheadaptationtosaltofmangrovefungiandtheirpotentialforbiotechnologicalapplications.
Productionofmannan-degradingenzymesfromAspergillusnigerandSclerotiumrolfsiiUsingpalmkernelcakeascarbonsource.
Abd-Aziz,S.,Ab-Razak,N.A.,Musa,M.H.&Hassam,M.A.(2009).ResearchJournalofEnvironmentalSciences,3(2),251-256.
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Theaimofthisstudyistoproduceandprofilethemannan-degradingenzymesfromlocalfungalisolatesbysubmergedfermentationandsaccharificationofPKC.ResultsshowedthatAspergillusnigerandSclerotiumrolfsiicanproducemannan-degradingenzymes.ThefungiweregrowninsubmergedfermentationofPKCtoproducemannan-degradingenzymes.Thehighestalpha-galactosidasewasobtainedonday13offermentation(0.128UmL-1)whenusingA.nigerandonday18(0.126UmL-1)whenusingS.rolfsii.Analysisalsoshowedthatenzymeactivitiesforbeta-mannanaseusingS.rolfsiiwerethehighestatday17(3.166UmL-1)andforA.niger(2.482UmL-1)atday8.Meanwhilethehighestbeta-mannosidasewereobtainedatday16forA.niger(0.128UmL-1)andforS.rolfsiiatday16(0.116UmL-1).
CrystallizationandpreliminaryX-raydiffractionstudiesofafamily26endo-β-1,4mannanase(ManA)fromPseudomonasfluorescenssubspeciescellulosa.
Scott,M.,Pickersgill,R.W.,Hazelwood,G.P.,Bolam,D.,Gilbert,H.J.&Harris,G.W.(1998).ActaCrystallographica,D54,129-131.
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Crystalsofanendo-β-1,4-mannanase(1,4-β-D-mannohydrolase,E.C.3.2.1.78)fromPseudomonasfluorescenssubspeciescellulosahavebeengrownbythehanging-droptechniqueat291Koveraperiodofonetotwoweekstomaximaldimensionsof0.17×0.17×0.25mm.ThesecrystalsbelongtothespacegroupR32(orR3)withcelldimensionsofa=b=155.4andc=250.8A(hexagonalsetting)andcontainthree(six)moleculesintheasymmetricunit.Thecrystalsdiffracttoatleast3.2/kusingalaboratorysourceandaresuitableforstructuredetermination.
Mannan-degradingenzymesfromCellulomonasfimi.
Stoll,D.Stalbrand,H.,Warren,R.A.J.(1999).JournalofAppliedandEnvironmentalMicrobiology,65(6),2598-2605.
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Thegenesman26aandman2AfromCellulomonasfimiencodemannanase26A(Man26A)andβ-mannosidase2A(Man2A),respectively.MatureMan26Aisasecreted,modularproteinof951aminoacids,comprisingacatalyticmoduleinfamily26ofglycosylhydrolases,anS-layerhomologymodule,andtwomodulesofunknownfunction.ExposureofMan26AproducedbyEscherichiacolitoC.fimiproteasegeneratesactivefragmentsoftheenzymethatcorrespondtopolypeptideswithmannanaseactivityproducedbyC.fimiduringgrowthonmannans,indicatingthatitmaybetheonlymannanaseproducedbytheorganism.AsignificantfractionoftheMan26AproducedbyC.fimiremainscellassociated.Man2Aisanintracellularenzymecomprisingacatalyticmoduleinasubfamilyoffamily2oftheglycosylhydrolasesthatatpresentcontainsonlymammalianβ-mannosidases.
品牌介绍
Megazyme品牌产品简介
来源:作者:人气:2149发表时间:2016-05-19 10:59:00【大 中 小】
Megazyme是一家全球性公司,专注于开发和提供用于饮料、谷物、乳制品、食品、饲料、发酵、生物燃料和葡萄酒产业用的分析试剂、酶和检测试剂盒。Megazyme的许多检测试剂盒产品已经为众多官方科学协会(包括AOAC, AACC , RACI, EBC和ICC等),经过严格的审核,批准认证为官方标准方法,确保以准确、可靠、定量和易于使用的测试方法,满足客户的质量诉求。
Megazyme的主要产品线包括:
◆ 检测试剂盒
◆ 酶
◆ 酶底物
◆ 碳水化合物
◆ 化学品/仪器
官网地址:http://www.megazyme.com
检测试剂盒特色产品:
货号
中文品名
用途
K-ACETAF
乙酸[AF法]检测试剂盒
酶法定量分析乙酸最广泛使用的方法
K-ACHDF
可吸收糖/膳食纤维检测试剂盒
酒精沉淀法测定膳食纤维
K-AMIAR
氨快速检测试剂盒
用于包括葡萄汁、葡萄酒以及其它食品饮料样品中氨含量的快速检测分析。
K-AMYL
直链淀粉/支链淀粉检测试剂盒
谷物淀粉和而粉中直链淀粉/支链淀粉比例和含量检测
K-ARAB
阿拉伯聚糖检测试剂盒
果汁浓缩液中阿拉伯聚糖的检测
K-ASNAM
L-天冬酰胺/L-谷氨酰胺和氨快速检测试剂盒
用于食品工业中丙烯酰胺前体、细胞培养基、以及上清液组分中、L-天冬酰胺,谷氨酰胺和氨的检测分析
K-ASPTM
阿斯巴甜检测试剂盒
专业用于测定饮料和食品中阿斯巴甜含量,操作简单
K-BETA3
β-淀粉酶检测试剂盒
适用于麦芽粉中β-淀粉酶的测定
K-BGLU
混合键β-葡聚糖检测试剂盒
测定谷物、荞麦粉、麦汁、啤酒及其它食品中混合键β-葡聚糖(1,3:1,4-β-D-葡聚糖)的含量
K-CERA
α-淀粉酶检测试剂盒
谷物和发酵液(真菌和细菌)中α-淀粉酶的分析测定
K-CITR
柠檬酸检测试剂盒
快速、可靠地检测食品、饮料和其它物料中柠檬酸(柠檬酸盐)含量
K-DLATE
乳酸快速检测试剂盒
快速、特异性检测饮料、肉类、奶制品和其它食品中L-乳酸和D-乳酸(乳酸盐)含量
K-EBHLG
酵母β-葡聚糖酶检测试剂盒
用于测量和分析酵母中1,3:1,6?-β-葡聚糖,也可以检测1,3-葡聚糖
K-ETSULPH
总亚硫酸检测试剂盒
测定葡萄酒、饮料、食品和其他物料中总亚硫酸含量(按二氧化硫计)的一种简单,高效,可靠的酶法检测方法
K-FRGLMQ
D-果糖/D-葡萄糖[MegaQuant法]检测试剂盒
适用于使用megaquant?色度计(505nm下)测定葡萄、葡萄汁和葡萄酒中D-果糖和D-葡萄糖的含量。
K-FRUC
果聚糖检测试剂盒
含有淀粉、蔗糖和其他糖类的植物提取物和食品中果聚糖的含量测定。
K-FRUGL
D-果糖/D-葡萄糖检测试剂盒
对植物和食品中果糖或葡萄糖含量的酶法紫外分光测定。
K-GALM
半乳甘露聚糖检测试剂盒
食品和植物产品中半乳甘露聚糖的含量检测
K-GLUC
D-葡萄糖[GOPOD]检测试剂盒
谷物提取物中D-葡萄糖的含量测定,可以和其它Megazyme检测试剂盒联合使用。
K-GLUHK
D-葡萄糖[HK]检测试剂盒
植物和食品中D-葡萄糖的含量测定,可以和其它Megazyme检测试剂盒联合使用。
K-GLUM
葡甘聚糖检测试剂盒
植物和食品中葡甘聚糖的含量测定。
K-INTDF
总膳食纤维检测试剂盒
总膳食纤维特定检测和分析
K-LACGAR
乳糖/D-半乳糖快速检测试剂盒
用于快速检测食品和植物产品中乳糖、D-半乳糖和L-阿拉伯糖
K-LACSU
乳糖/蔗糖/D-葡萄糖检测试剂盒
混合面粉和其它物料中蔗糖、乳糖和D-葡萄糖的测定
K-LACTUL
乳果糖检测试剂盒
特异性、快速和灵敏测量奶基样品中乳果糖含量
K-MANGL
D-甘露糖/D-果糖/D-葡萄糖检测试剂盒
适合测定植物产品和多糖酸性水解产物中D-甘露糖含量
K-MASUG
麦芽糖/蔗糖/D-葡萄糖检测试剂盒
在植物和食品中麦芽糖,蔗糖和葡萄糖的含量检测
K-PECID
胶质识别检测试剂盒
食品配料中果胶的鉴别
K-PHYT
植酸(总磷)检测试剂盒
食品和饲料样品植酸/总磷含量测量的简便方法。不需要通过阴离子交换色谱对植酸纯化,适合于大量样本分析
K-PYRUV
丙酮酸检测试剂盒
在啤酒、葡萄酒、果汁、食品和体液中丙酮酸分析
K-RAFGA
棉子糖/D-半乳糖检测试剂盒
快速测量植物材料和食品中棉子糖和半乳糖含量
K-RAFGL
棉子糖/蔗糖/D-半乳糖检测试剂盒
分析种子和种子粉中D-葡萄糖、蔗糖、棉子糖、水苏糖和毛蕊花糖含量。通过将棉子糖、水苏糖和毛蕊花糖酶解D-葡萄糖、D-果糖和半乳糖,从而测定葡萄糖含量来确定
K-SDAM
淀粉损伤检测试剂盒
谷物面粉中淀粉损伤的检测和分析
K-SUCGL
蔗糖/D-葡萄糖检测试剂盒
饮料、果汁、蜂蜜和食品中蔗糖和葡萄糖的分析
K-SUFRG
蔗糖/D-果糖/D-葡萄糖检测试剂盒
适用于植物和食品中蔗糖、D-葡萄糖和D-果糖的测定
K-TDFR
总膳食纤维检测试剂盒
总膳食纤维检测
K-TREH
海藻糖检测试剂盒
快速、可靠地检测食品、饮料和其它物料中海藻糖含量
K-URAMR
尿素/氨快速检测试剂盒
适用于水、饮料、乳制品和食品中尿素和氨的快速测定
K-URONIC
D-葡萄糖醛酸/D-半乳糖醛酸检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中六元糖醛酸含量(D-葡萄糖醛酸和D-半乳糖醛酸)
K-XYLOSE
D-木糖检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中D-木糖含量
K-YBGL
Beta葡聚糖[酵母和蘑菇]检测试剂盒
检测酵母和蘑菇制品中1,3:1,6-beta-葡聚糖和α-葡聚糖含量
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