Megazyme/线性1,5-&α-L-阿拉伯糖（甜菜）/P-LARB/500毫克
商品编号：
P-LARB
品牌：
Megazyme INC
市场价：
¥3576.00
美元价：
2145.60
产品分类：
其他试剂
公司分类：
Other_reagents
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
HighpurityLinear1,5-α-L-ArABInan(SugarBeet)foruseinresearch,biochemicalenzymeassaysandinvitrodiagnosticanalysis.
Purity>95%.Caroxymethylated(DS=0.05),highlypurifiedlinear1,5-α-L-arabinan.Fortheassayofendo-1,5-α-L-arabinanase(100assays/vial;methodsheetsupplied).
Developmentalcomplexityofarabinanpolysaccharidesandtheirprocessinginplantcellwalls.
Verhertbruggen,Y.,Marcus,S.E.,Haeger,A.,Verhoef,R.,Schols,H.A.,McCleary,B.V.,McKee,L.,Gilbert,H.J.&PaulKnox,J.(2009).ThePlantJournal,59(3),413-425.
LinktoArticle
ReadAbstract
Plantcellwallsareconstructedfromadiversityofpolysaccharidecomponents.Molecularprobesdirectedtostructuralelementsofthesepolymersarerequiredtoassaypolysaccharidestructuresinsitu,andtodeterminepolymerrolesinthecontextofcellwallBIOLOGy.Here,wereportontheisolationandthecharacterizationofthreeratmonoclonalantibodiesthataredirectedto1,5-linkedarabinansandrelatedpolymers.LM13,LM16andLM17,togetherwithLM6,constituteasetofantibodiesthatcandetectdifferingaspectsofarabinanstructureswithincellwalls.EachoftheseantibodiesbindsstronglytoisolatedsugarbeetarabinansamplesinELISAs.Competitive-inhibitionELISAsindicatetheantibodiesbinddifferentiallytoarabinanswiththebindingofLM6andLM17beingeffectivelyinhibitedbyshortoligoarabinosides.LM13bindspreferentiallytolongeroligoarabinosides,anditsbindingishighlysensitivetoarabinanaseaction,indicatingtherecognitionofalongerlinearizedarabinanepitope.Incontrast,thebindingofLM16tobranchedarabinanandtocellwallsisincreasedbyarabinofuranosidaseaction.Thepresenceofallepitopescanbedifferentiallymodulatedinvitrousingglycosidehydrolasefamily43andfamily51arabinofuranosidases.Inaddition,theLM16epitopeissensitivetotheactionofβ-galactosidase.Immunofluorescencemicroscopyindicatesthattheantibodiescanbeusedtodetectepitopesincellwalls,andthatthefourantibodiesrevealcomplexpatternsofepitopeoccurrencethatvarybetweenorgansandspecies,andrelatebothtotheprobableprocessingofarabinanstructuralelementsandthedifferingmechanicalpropertiesofcellwalls.
Cellulosemicrofibrilanglesandcell-wallpolymersindifferentwoodtypesofPinusrADIata.
Brennan,M.,McLean,J.P.,Altaner,C.M.,Ralph,J.&Harris,P.J.(2012).Cellulose,19(4),1385-1404.
LinktoArticle
ReadAbstract
FourcorewoodtypeswereexaminedfromsaplingtreesoftwoclonesofPinusradiatagrowninaglasshouse.Treesweregrowneitherstraighttoproducenormalcorewood,tiltedat45°fromtheverticaltoproduceoppositecorewoodandcompressioncorewood,orrockedtoproduceflexurecorewood.MeancellulosemicrofibrilangleoftracheidwallswasestimatedbyX-raydiffractionandlongitudinalswellingmeasuredbetweenanovendryandmoisturesaturatedstate.Ligninandacetylcontentsofthewoodsweremeasuredandthemonosaccharidecompositionsofthecell-wallpolysaccharidesdetermined.Finelymilledwoodwasanalysedusingsolution-state2DNMRspectroscopyofgelsfromfinelymilledwoodinDMSO-d6/pyridine-d5.Althoughtherewasnosignificantdifferenceincellulosemicrofibrilangleamongthecorewoodtypes,compressioncorewoodhadthehighestlongitudinalswelling.Alignincontent>32%andagalactosylresiduecontent>6%clearlydividedseverecompressioncorewoodfromtheothercorewoodtypes.Relationshipscouldbedrawnbetweenlignincontentandlongitudinalswelling,andbetweengalactosylresiduecontentandlongitudinalswelling.The2DNMRspectrashowedthatthepresenceofH-unitsinligninwasexclusivetocompressioncorewood,whichalsohadahigher(1→4)-β-D-galactancontent,definingauniquecompositionforthatcorewoodtype.
PurificationandcharacterizationofaxylanaseandanarabinofuranosidasefromBacilluspolymyxa.
Morales,P.,Madarro,A.,Flors,A.,Sendra,J.&Pérez-González,J.(1995).EnzymeandMicrobialTechnology,17(5),424-429.
LinktoArticle
ReadAbstract
TwohemicellulasesfromBacilluspolymyxawerepurifiedandcharacterized:axylanasewithamolecularmassof61kDandplof4.7andanarabinofuranosidasewithamolecularmassof166kDandplof4.7.Thexylanase,whichshowedincreasedThermostabilityinthepresenceofMgCl2,showedatypicalendo-actionmodeonxylansfromseveralsources.Thearabinofuranosidasewasonlyactiveon(1→5)-α-L-arabinooligosaccharidesbutnotonlinear(1→5)-α-L-arabinan,arabinogalactan,andarabinoxylan.However,itwasabletoreleasearabinosefromarabinoxylanwhenanactiveendoxylanasewasalsopresentinhydrolysisassays.
α-L-ArabinofuranosidasesfromAspergillusterreuswithpotentialapplicationinenology:induction,purification,andcharacterization.
LeClinche,F.,Piñaga,F.,Ramón,D.&Vallés,S.(1997).JournalofAgriculturalandFoodChemistry,45(7),2379-2383.
LinktoArticle
ReadAbstract
InthepresenceofL-arabitolassolecarbonsource,AspergillusterreusCECT2663producesthreeα-L-arabinofuranosidases(ABFs)namedABF1,ABF2,andABF3,withmolecularmassesof90 000,82 000,and78 500Da,respectively.Thesynthesisoftheseenzymesisundercarboncataboliterepression.WesternblottingrevealedthatABF2isimmunologicallyrelatedtotheα-L-arabinofuranosidaseBpreviouslyisolatedfromAspergillusniger.ThethreeA.terreusproteinshavebeenpurifiedtohomogeneity.TheyareacidicproteinswithoptimalpHsof5.0forABF1andABF2and5.5forABF3andoptimaltemperaturesof50,60,and65°C,respectively.Kineticconstantsforthepurifiedenzymesonp-nitrophenylα-L-arabinofuranoside(pNPA)assubstratehavebeendetermined.Thethreeenzymesmaintainelevatedactivitiesinthepresenceofethanolorglucoseatthoseconcentrationsnormallypresentinmustorwine.
Near-infraredFourier-transformRamanspectroscopyofflax(LinumusitatissimumL.)stems.
Himmelsbach,D.S.&Akin,D.E.(1998).JournalofAgriculturalandFoodChemistry,46(3),991-998.
LinktoArticle
ReadAbstract
Samplesofflax(LinumusitatissimumL.)stemanditsanatomicalpartswerestudiedbynear-infraredFouriertransformRaman(NIR-FT-Raman)spectroscopytodetermineifthemajorchemicalcomponentsofeachcouldbedetectedbythismethod.TheRamanspectraofreferencecompoundsfromrelativelypurematerialsservedasmodelsforthechemicalcomponents.Bandsforcelluloseweregreatestinthefibers.Hemicellulosicpolysaccharideswereobservedtobeprevalentinbasttissueandfibers.Weaksignalsforpectinswereobservedinthebast,cuticle/epidermis,fibers,andstem.Bandsforaromaticringsweredetectableinallmaterials.Bandsfromwaxes/fattyacidestersweredetectableinthecuticle/epidermaltissue.TheresultsindicatedthatNIR-FT-Ramancouldbeuseddetectthemajorchemicalcomponentsinflaxinsituandprovideasimple,rapid,andnoninvasiveassessmentoftheirrelativeamountsandlocationwithinthetissuesoftheflaxplant.
Purification,functionalcharacterization,cloning,andidentificationofmutantsofaseed-specificarabinanhydrolaseinArabidopsis.
Minic,Z.,Do,C.T.,Rihouey,C.,Morin,H.,Lerouge,P.&Jouanin,L.(2006).JournalofExperimentalBotany,57(10),2339-2351.
LinktoArticle
ReadAbstract
ThisworkdescribesthepurificationandcharacterizationofanenzymethatexhibitsarabinanhydrolaseactivityinseedsofArabidopsisthaliana.Theenzyme,designatedXYL3,hadanapparentmolecularmassof80kDawhenpurifiedtohomogeneity,andwasidentifiedusingMALDI-TOF(matrix-assistedlaserdesorptionionization–timeofflight)asaputativeβ-D-xylosidasethatbelongstofamily3ofglycosidehydrolasesencodedbygeneAt5g09730.XYL3hydrolysedsyntheticsubstratessuchasp-nitrophenyl-α-L-arabinofuranosideandp-nitrophenyl-β-D-xylosidewithsimilarcatalyticefficiency.XYL3releasedL-arabinosefrom(1→5)-α-L-arabinofuranobiose,arabinoxylan,sugarbeetarabinan,anddebranchedarabinan.Theenzymehydrolysedbotharabinosyl-substitutedsidegroupresiduesandterminalarabinofuranosylresidues(1→5)-α-linkedtothearabinanbackbone.ThisindicatesthatXYL3isabletodegradeallterminalarabinosylresiduesandsuggeststhatitparticipatesinthein-vivohydrolysisofarabinan.Analysisofgeneexpressionpatternsbysemi-quantitativeRT-PCR,in-situhybridizationandapromoter–GUSfusiondemonstratedthatAtBX3wasspecificallyexpressedintheseedendospermattheglobularstageoftheembryo.ImmunolocalizationusingLM6anti-arabinanantiserafoundthatarabinan,theXYL3substrate,wasalsopresentinthisseedtissue.T-DNAnullmutantsforAtBX3wereidentified.Themutantplantslackedtheα-L-arabinofuranosidaseandβ-D-xylosidaseactivitiescorrespondingtoXYL3.Mutantsshowedreducedseedsizeandaredelayedinseedlinggerminationcomparedwiththewildtype.
CharacterizationandcomparisonofpolysaccharidesfromLyciumbarbaruminChinausingsaccharidemappingbasedonPACEandHPTLC.
Wu,D.T.,Cheong,K.L.,Deng,Y.,Lin,P.C.,Wei,F.,Lv,X.J.,Long,Z,R.,Zhao,J.,Ma,S,C.&Li,S.P.(2015).CarbohydratePolymers,134,12-19.
LinktoArticle
ReadAbstract
Water-solublepolysaccharidesfrom51batchesoffruitsofL.barbarum(wolfberry)inChinawereinvestigatedandcomparedusingsaccharidemapping,partialacidhydrolysis,singleandcompositeenzymaticdigestion,followedbypolysaccharideanalysisbyusingcarbohydrategelelectrophoresis(PACE)analysisandhighperformancethinlayerchromatography(HPTLC)analysis,respectively.ResultsshowedthatmultiplePACEandHPTLCfingerprintsofpartialacidandenzymatichydrolysatesofpolysaccharidesfromL.barbaruminChinaweresimilar,respectively.Inaddition,resultsindicatedthatβ-1,3-glucosidic,α-1,4-galactosIduronicandα-1,5-arabinosidiclinkagesexistedinpolysaccharidesfrom L.barbarumcollectedinChina,andthesimilarityofpolysaccharidesinL.barbarumcollectedfromdifferentregionsofChinawasprettyhigh,whicharehelpfulfortheimprovementoftheperformanceofpolysaccharidesfrom L.barbaruminfunctional/healthfoodsarea.Furthermore,polysaccharidesfromPanaxnotoginseng,Angelicasinensis,andAstragalusmembranaceusvar.mongholicusweresuccessfullydistinguishedfromthoseof L.barbarumbasedontheirPACEfingerprints.Theseresultswerebeneficialtoimprovethequalitycontrolofpolysaccharidesfrom L.barbarumandtheirproducts,whichsuggestedthatsaccharidemappingbasedonPACEandHPTLCanalysiscouldbearoutineapproachforqualitycontrolofpolysaccharides.
Enzymaticpecticoligosaccharides(POS)productionfromsugarbeetpulpusingresponsesurfacemethodology.
Babbar,N.,Dejonghe,W.,Sforza,S.&Elst,K.(2017).JournalofFoodScienceandTechnology,1-9.
LinktoArticle
ReadAbstract
Pecticoligosaccharides(POS)havebeenindicatedasnovelcandidateprebiotics.Traditionally,POSareproducedfrompectin-richby-productsusingatwo-stepprocessinvolvingextractionofthepectin,followedbyitshydrolysisintoPOS.Aone-stepapproach,inwhichthePOSisdirectlyproducedfromtherawmaterial,mightprovideamoreefficientalternative.Thus,themainaimofthispaperwastoinvestigateaone-stepenzymatichydrolysisapproachtodirectlyproducePOSfromsugarbeetpulp(SBP).ThePOSyieldwasinvestigatedasafunctionoftheprocessparameters,aswellasrawmaterialcharacteristics.Astatistically-basedresponsesurfacemethodology,usingacentralcompositedesignwasapplied,toinvestigatetheindividualaswellasthecombinedinfluencesofthediverseparameters.Themodelwasconfirmedbyavalidationexperiment,carriedoutat135g/lsubstrateconcentration,0.75FPU/gSBPenzymeconcentration,0.8mmparticlesizeand3hhydrolysistime.Undertheseconditions,aPOS-richhydrolysatewasobtained,containingrhamnose,arabinose,galactose,xyloseandgalacturonicacid,at0.9,15.2,5.1,1.4,and13.2g/l,respectively,enzymeswereaddedeachat20FPU/gdrymatter(DM).
品牌介绍
Megazyme品牌产品简介
来源：作者：人气：2149发表时间：2016-05-19 10:59:00【大 中 小】
Megazyme是一家全球性公司，专注于开发和提供用于饮料、谷物、乳制品、食品、饲料、发酵、生物燃料和葡萄酒产业用的分析试剂、酶和检测试剂盒。Megazyme的许多检测试剂盒产品已经为众多官方科学协会（包括AOAC, AACC , RACI, EBC和ICC等），经过严格的审核，批准认证为官方标准方法，确保以准确、可靠、定量和易于使用的测试方法，满足客户的质量诉求。
Megazyme的主要产品线包括：
◆ 检测试剂盒
◆ 酶
◆ 酶底物
◆ 碳水化合物
◆ 化学品/仪器
官网地址：http://www.megazyme.com
检测试剂盒特色产品：
货号
中文品名
用途
K-ACETAF
乙酸[AF法]检测试剂盒
酶法定量分析乙酸最广泛使用的方法
K-ACHDF
可吸收糖/膳食纤维检测试剂盒
酒精沉淀法测定膳食纤维
K-AMIAR
氨快速检测试剂盒
用于包括葡萄汁、葡萄酒以及其它食品饮料样品中氨含量的快速检测分析。
K-AMYL
直链淀粉/支链淀粉检测试剂盒
谷物淀粉和而粉中直链淀粉/支链淀粉比例和含量检测
K-ARAB
阿拉伯聚糖检测试剂盒
果汁浓缩液中阿拉伯聚糖的检测
K-ASNAM
L-天冬酰胺/L-谷氨酰胺和氨快速检测试剂盒
用于食品工业中丙烯酰胺前体、细胞培养基、以及上清液组分中、L-天冬酰胺，谷氨酰胺和氨的检测分析
K-ASPTM
阿斯巴甜检测试剂盒
专业用于测定饮料和食品中阿斯巴甜含量，操作简单
K-BETA3
β-淀粉酶检测试剂盒
适用于麦芽粉中β-淀粉酶的测定
K-BGLU
混合键β-葡聚糖检测试剂盒
测定谷物、荞麦粉、麦汁、啤酒及其它食品中混合键β-葡聚糖(1,3:1,4-β-D-葡聚糖)的含量
K-CERA
α-淀粉酶检测试剂盒
谷物和发酵液（真菌和细菌）中α-淀粉酶的分析测定
K-CITR
柠檬酸检测试剂盒
快速、可靠地检测食品、饮料和其它物料中柠檬酸（柠檬酸盐）含量
K-DLATE
乳酸快速检测试剂盒
快速、特异性检测饮料、肉类、奶制品和其它食品中L-乳酸和D-乳酸(乳酸盐）含量
K-EBHLG
酵母β-葡聚糖酶检测试剂盒
用于测量和分析酵母中1,3:1,6?-β-葡聚糖，也可以检测1,3-葡聚糖
K-ETSULPH
总亚硫酸检测试剂盒
测定葡萄酒、饮料、食品和其他物料中总亚硫酸含量（按二氧化硫计）的一种简单，高效，可靠的酶法检测方法
K-FRGLMQ
D-果糖/D-葡萄糖[MegaQuant法]检测试剂盒
适用于使用megaquant?色度计（505nm下）测定葡萄、葡萄汁和葡萄酒中D-果糖和D-葡萄糖的含量。
K-FRUC
果聚糖检测试剂盒
含有淀粉、蔗糖和其他糖类的植物提取物和食品中果聚糖的含量测定。
K-FRUGL
D-果糖/D-葡萄糖检测试剂盒
对植物和食品中果糖或葡萄糖含量的酶法紫外分光测定。
K-GALM
半乳甘露聚糖检测试剂盒
食品和植物产品中半乳甘露聚糖的含量检测
K-GLUC
D-葡萄糖[GOPOD]检测试剂盒
谷物提取物中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUHK
D-葡萄糖[HK]检测试剂盒
植物和食品中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUM
葡甘聚糖检测试剂盒
植物和食品中葡甘聚糖的含量测定。
K-INTDF
总膳食纤维检测试剂盒
总膳食纤维特定检测和分析
K-LACGAR
乳糖/D-半乳糖快速检测试剂盒
用于快速检测食品和植物产品中乳糖、D-半乳糖和L-阿拉伯糖
K-LACSU
乳糖/蔗糖/D-葡萄糖检测试剂盒
混合面粉和其它物料中蔗糖、乳糖和D-葡萄糖的测定
K-LACTUL
乳果糖检测试剂盒
特异性、快速和灵敏测量奶基样品中乳果糖含量
K-MANGL
D-甘露糖/D-果糖/D-葡萄糖检测试剂盒
适合测定植物产品和多糖酸性水解产物中D-甘露糖含量
K-MASUG
麦芽糖/蔗糖/D-葡萄糖检测试剂盒
在植物和食品中麦芽糖，蔗糖和葡萄糖的含量检测
K-PECID
胶质识别检测试剂盒
食品配料中果胶的鉴别
K-PHYT
植酸(总磷)检测试剂盒
食品和饲料样品植酸/总磷含量测量的简便方法。不需要通过阴离子交换色谱对植酸纯化，适合于大量样本分析
K-PYRUV
丙酮酸检测试剂盒
在啤酒、葡萄酒、果汁、食品和体液中丙酮酸分析
K-RAFGA
棉子糖/D-半乳糖检测试剂盒
快速测量植物材料和食品中棉子糖和半乳糖含量
K-RAFGL
棉子糖/蔗糖/D-半乳糖检测试剂盒
分析种子和种子粉中D-葡萄糖、蔗糖、棉子糖、水苏糖和毛蕊花糖含量。通过将棉子糖、水苏糖和毛蕊花糖酶解D-葡萄糖、D-果糖和半乳糖，从而测定葡萄糖含量来确定
K-SDAM
淀粉损伤检测试剂盒
谷物面粉中淀粉损伤的检测和分析
K-SUCGL
蔗糖/D-葡萄糖检测试剂盒
饮料、果汁、蜂蜜和食品中蔗糖和葡萄糖的分析
K-SUFRG
蔗糖/D-果糖/D-葡萄糖检测试剂盒
适用于植物和食品中蔗糖、D-葡萄糖和D-果糖的测定
K-TDFR
总膳食纤维检测试剂盒
总膳食纤维检测
K-TREH
海藻糖检测试剂盒
快速、可靠地检测食品、饮料和其它物料中海藻糖含量
K-URAMR
尿素/氨快速检测试剂盒
适用于水、饮料、乳制品和食品中尿素和氨的快速测定
K-URONIC
D-葡萄糖醛酸/D-半乳糖醛酸检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中六元糖醛酸含量（D-葡萄糖醛酸和D-半乳糖醛酸）
K-XYLOSE
D-木糖检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中D-木糖含量
K-YBGL
Beta葡聚糖[酵母和蘑菇]检测试剂盒
检测酵母和蘑菇制品中1,3:1,6-beta-葡聚糖和α-葡聚糖含量
联络我们
