Megazyme/阿拉伯木聚糖（小麦粉；低粘度~10 cSt）/P-蜡基/3克
商品编号：
P-WAXYL
品牌：
Megazyme INC
市场价：
¥3576.00
美元价：
2145.60
产品分类：
其他试剂
公司分类：
Other_reagents
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
HighpurityArABInoxylan(WheatFlour;LowViscosity~10CST)foruseinresearch,biochemicalenzymeassaysand in vitro diagnosticanalysis.
Purity~95%.Viscosity~10cSt.Ara:Xyl=38:62.Glucose,galactoseandmannose<1%.
Novelsubstratesfortheautomatedandmanualassayofendo-1,4-β-xylanase.
Mangan,D.,Cornaggia,C.,Liadova,A.,McCormack,N.,Ivory,R.,McKie,V.A.,Ormerod,A.&McCleary,D.V.(2017).CarbohydrateResearch,445,14-22.
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endo-1,4-β-Xylanase(EC3.2.1.8)isemployedacrossabroadrangeofindustriesincludinganimalfeed,brewing,baking,biofuels,detergentsandpulp(paper).Despiteitsimportance,arapid,reliable,reproducIBLe,automatableassayforthisenzymethatisbasedontheuseofachemicallydefinedsubstratehasnotbeendescribedtodate.Reportedhereinisanewenzymecoupledassayprocedure,termedtheXylX6assay,thatemploysanovelsubstrate,namely4,6-O-(3-ketobutylidene)-4-nitrophenyl-β-45-O-glucosyl-xylopentaoside.ThedevelopmentofthesubstrateandassociatedassayisdiscussedhereandtherelationshipbetweentheactivityvaluesobtainedwiththeXylX6assayversustrADItionalreducingsugarassaysanditsspecificityandreproducibilitywerethoroughlyinvestigated.
Hydrolysisofwheatflourarabinoxylan,acid-debranchedwheatflourarabinoxylanandarabino-xylo-oligosaccharidesbyβ-xylanase,α-L-arabinofuranosidaseandβ-xylosidase.
McCleary,B.V.,McKie,V.A.,Draga,A.,Rooney,E.,Mangan,D.&Larkin,J.(2015).CarbohydrateResearch,407,79-96.
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Arangeofα-L-arabinofuranosyl-(1-4)-β-D-xylo-oligosaccharides(AXOS)wereproducedbyhydrolysisofwheatflourarabinoxylan(WAX)andaciddebranchedarabinoxylan(ADWAX),inthepresenceandabsenceofanAXH-d3α-L-arabinofuranosidase,byseveralGH10andGH11β-xylanases.ThestructuresoftheoligosaccharideswerecharacterisedbyGC-MSandNMRandbyhydrolysisbyarangeofα-L-arabinofuranosidasesandβ-xylosidase.TheAXOSwerepurifiedandusedtocharacterisetheactionpatternsofthespecificα-L-arabinofuranosidases.Theseenzymes,incombinationwitheitherCellvibriomixtusorNeocallimastixpatriciarumβ-xylanase,wereusedtoproduceelevatedlevelsofspecificAXOSonhydrolysisofWAX,suchas32-α-L-Araf-(1-4)-β-D-xylobiose(A3X),23-α-L-Araf-(1-4)-β-D-xylotriose(A2XX),33-α-L-Araf-(1-4)-β-D-xylotriose(A3XX),22-α-L-Araf-(1-4)-β-D-xylotriose(XA2X),32-α-L-Araf(1-4)-β-D-xylotriose(XA3X),23-α-L-Araf-(1-4)-β-D-xylotetraose(XA2XX),33-α-L-Araf-(1-4)-β-D-xylotetraose(XA3XX),23,33-di-α-L-Araf-(1-4)-β-D-xylotriose(A2+3XX),23,33-di-α-L-Araf-(1-4)-β-D-xylotetraose(XA2+3XX),24,34-di-α-L-Araf-(1-4)-β-D-xylopentaose(XA2+3XXX)and33,34-di-α-L-Araf-(1-4)-β-D-xylopentaose(XA3A3XX),manyofwhichhavenotpreviouslybeenproducedinsufficientquantitiestoallowtheiruseassubstratesinfurtherenzymicstudies.ForA2,3XX,yieldsofapproximately16%ofthestartingmaterial(wheatarabinoxylan)havebeenachieved.Mixturesoftheα-L-arabinofuranosidases,withspecificactiononAXOS,havebeencombinedwithβ-xylosidaseandβ-xylanasetoobtainanoptimalmixtureforhydrolysisofarabinoxylantoL-arabinoseandD-xylose.
Preparationofarabinoxylobiosefromryexylanusingfamily10Aspergillusaculeatusendo-1,4-β-D-xylanase.
Rantanen,H.,Virkki,L.,Tuomainen,P.,Kabel,M.,Schols,H.&Tenkanen,M.(2007).CarbohydratePolymers,68(2),350-359.
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CommercialxylanasepreparationShearzyme®,whichcontainstheglycosidehydrolasefamily10endo-1,4-β-D-xylanasefromAspergillusaculeatus,wasusedtoprepareshort-chainarabinoxylo-oligosaccharides(AXOS)fromryearabinoxylan(AX).AmajorAXOSwasformedasahydrolysisproduct.LongerAXOSwerealsoproducedasminorproducts.ThepureGH10xylanasefromA.aculeatuswasusedasacomparisontoensurethattheformedAXOSwereconsequenceoftheendoxylanase‘sfunctioninsteadofsomesideenzymespresentinShearzyme.ThemajorAXOSwaspurifiedandthestructureconfirmedwithvariousanalysismethods(TLC,HPAEC-PAD,MALDI-TOF-MS,andone-andtwo-dimensionalNMRspectroscopywithnano-probe)asα-L-Araf-(1→3)-β-D-Xylp-(1→4)-D-Xylp(arabinoxylobiose).Thisisthefirstreporton13CNMRdataofpurearabinoxylobiose.Theyieldofarabinoxylobiosewas12%fromthequantifiedhydrolysisproducts.Inconclusion,GH10endoxylanasefromA.aculeatusisthusabletocutefficientlythexylosidiclinkagenexttothearabinofuranosyl-substitutedxyloseunitwhichisnottypicalforalltheGH10endoxylanases.Interestingly,pureA.aculeatusxylanaseshowednotablyactivitytowardsp-nitrophenyl-β-D-xylopyranose.InpreviouslystudieslongerAXOShavebeenproducedwithShearzymebuttheformationofshort-chainAXOSbyA.aculeatusGH10xylanasehasnotbeenstudiedbefore.
CompletegenomeofanewFirmicutesspeciesbelongingtothedominanthumancolonicmicrobiota(‘Ruminococcusbicirculans’)revealstwochromosomesandaselectivecapacitytoutilizeplantglucans.
Wegmann,U.,Louis,P.,Goesmann,A.,Henrissat,B.,Duncan,S.H.&Flint,H.J.(2014).EnvironmentalMicroBIOLOGy,16(9),2879–2890.
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Therecentlyisolatedbacterialstrain80/3representsoneofthemostabundant16SrRNAphylotypesdetectedinthehealthyhumanlargeintestineandbelongstotheRuminococcaceaefamilyofFirmicutes.Thecompletedgenomesequencereportedhereisthefirstforamemberofthisimportantfamilyofbacteriafromthehumancolon.Thegenomecomprisestwolargechromosomesof2.24and0.73Mbp,leadingustoproposethenameRuminococcusbicirculansforthisnewspecies.Analysisofthecarbohydrateactiveenzymecomplementsuggestsanabilitytoutilizecertainhemicelluloses,especiallyβ-glucansandxyloglucan,forgrowththatwasconfirmedexperimentally.Theenzymaticmachineryenablingthedegradationofcelluloseandxylanbyrelatedcellulolyticruminococciishoweverlackinginthisspecies.Whilethegenomeindicatedthecapacitytosynthesizepurines,pyrimidinesandall20aminoacids,onlygenesforthesynthesisofnicotinate,NAD+,NADP+andcoenzymeAweredetectedamongtheessentialvitaminsandco-factors,resultinginmultiplegrowthrequirements.Invivo,thesegrowthfactorsmustbesuppliedfromthediet,hostorothergutmicroorganisms.OtherfeaturesofecologicalinterestincludetwotypeIVpilins,multipleextracytoplasmicfunction-sigmafactors,aureaseandabilesalthydrolase.
Arevisedarchitectureofprimarycellwallsbasedonbiomechanicalchangesinducedbysubstrate-specificendoglucanases.
Park,Y.B.&Cosgrove,D.J.(2012).PlantPhysiology,158(4),1933-1943.
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Xyloglucaniswidelybelievedtofunctionasatetherbetweencellulosemicrofibrilsintheprimarycellwall,limitingcellenlargementbyrestrictingtheabilityofmicrofibrilstoseparatelaterally.Totestthebiomechanicalpredictionsofthis“tetherednetwork”model,weassessedtheabilityofcucumber(Cucumissativus)hypocotylwallstoundergocreep(long-term,irreversibleextension)inresponsetothreefamily-12endo-β-1,4-glucanasesthatcanspecificallyhydrolyzexyloglucan,cellulose,orboth.Xyloglucan-specificendoglucanase(XEGfromAspergillusaculeatus)failedtoinducecellwallcreep,whereasanendoglucanasethathydrolyzesbothxyloglucanandcellulose(Cel12AfromHypocreajecorina)inducedahighcreeprate.Acellulose-specificendoglucanase(CEGfromAspergillusniger)didnotcausecellwallcreep,eitherbyitselforincombinationwithXEG.Testswithadditionalenzymes,includingafamily-5endoglucanase,confirmedtheconclusionthattocausecreep,endoglucanasesmustcutbothxyloglucanandcellulose.Similarresultswereobtainedwithmeasurementsofelasticandplasticcompliance.BothXEGandCel12Ahydrolyzedxyloglucaninintactwalls,butCel12AcouldhydrolyzeaminorxyloglucancompartmentrecalcitranttoXEGdigestion.XyloglucaninvolvementintheseenzymeresponseswasconfirmedbyexperimentswithArabidopsis(Arabidopsisthaliana)hypocotyls,whereCel12Ainducedcreepinwild-typebutnotinxyloglucan-deficient(xxt1/xxt2)walls.Ourresultsareincompatiblewiththecommondepictionofxyloglucanasaload-bearingtetherspanningthe20-to40-nmspacingbetweencellulosemicrofibrils,buttheydoimplicateaminorxyloglucancomponentinwallmechanics.Thestructurallyimportantxyloglucanmaybelocatedinlimitedregionsoftightcontactbetweenmicrofibrils.
Structuralbasisforentropy-drivencellulosebindingbyatype-Acellulose-bindingmodule(CBM)andbacterialexpansin.
Georgelis,N.,Yennawar,N.H.&Cosgrove,D.J.(2012).ProceedingsoftheNationalAcademyofSciences,109(37),14830-14835.
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Componentsofmodularcellulases,type-Acellulose-bindingmodules(CBMs)bindtocrystallinecelluloseandenhanceenzymeeffectiveness,butstructuraldetailsoftheinteractionareuncertain.WeanalyzedcellulosebindingbyEXLX1,abacterialexpansinwithabilitytoloosenplantcellwallsandwhosedomainD2hastype-ACBMcharacteristics.EXLX1stronglybindstocrystallinecelluloseviaD2,whereasitsaffinityforsolublecellooligosaccharidesisweak.Calorimetryindicatedcellulosebindingwaslargelyentropicallydriven.WesolvedthecrystalstructuresofEXLX1complexedwithcellulose-likeoligosaccharidestofindthatEXLX1bindstheligandsthroughhydrophobicinteractionsofthreelinearlyarrangedaromaticresiduesinD2.Thecrystalstructuresrevealedauniqueformofligand-mediateddimerization,withtheoligosaccharidesandwichedbetweentwoD2domainsinoppositepolarity.Thisreportclarifiesthemoleculartargetofexpansinandthespecificmolecularinteractionsofatype-ACBMwithcellulose.
Characterizationofanewα-L-arabinofuranosidasefromPenicilliumsp.LYG0704,andtheirapplicationinlignocellulosesdegradation.
Lee,D.S.,Wi,S.G.,Lee,Y.G.,Cho,E.J.,Chung,B.Y.&Bae,H.J.(2011).MolecularBiotechnology,49(3),229-239.
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Agene(arf)encodinganα-L-arabinofuranosidase(ARF)thathydrolyzesarabinosesubstitutedonxylanwasisolatedfromPenicilliumsp.Thegenewaspredictedtoencode339aminoacidresiduesshowing71–75%homologytoGHfamily54.E.coliexpressedARFshowedoptimalactivityat50°CandpH5–6onwheatarabinoxylan.ThehydrolysisactivitiesonoatspeltxylanbyARFandxylanasewere1.67-foldhigherthanthatofxylanasealone.ThesynergisticeffectsofARFandcommercialenzymes(xylanaseandcellulase)onpopping-pretreatedricestrawwere1.15–1.51-foldhigheramountsofsugarsreleasedinthe[ARF+xylanase+cellulase]mixturethaninthemixtures[ARF+xylanase],[ARF+cellulase],and[xylanase+cellulase].Moreover,theliberationofarabinosebyARFwasenhanced2.1–2.9-foldinareactionwithxylanaseandcellulaseascomparedwith[xylanase+cellulase]andARFalone.
Invitrofermentationkineticsandend-productsofcerealarabinoxylansand(1,3;1,4)-β-glucansbyporcinefaeces.
Williams,B.A.,Mikkelsen,D.,LePaih,L.&Gidley,M.J.(2011).Journalofcerealscience,53(1),53-58.
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Purifiedandsemi-purifiedpolysaccharidescharacteristicofcerealswerefermentedinvitrowithapigfaecalinoculum,usingthecumulativegasproductiontechnique,toexaminethekineticsandend-productsoffermentationafter48h.Itwasshownthatarabinoxylanandmixedlinkage(1,3;1,4)β-glucanwererapidlyfermentedifsoluble,whilelesssolublesubstrates(insolublearabinoxylan,maizeandwheatstarchgranules,andbacterialcellulose)weremoreslowlyfermented.Relevantmonosaccharideswerefermentedatverysimilarratestosolublepolymericarabinoxylanandβ-glucan,showingthatdepolymerisationwasnotalimitingstep,incontrasttosomepreviousstudies.Bacterialcelluloseisshowntobeausefulmodelsubstrateforfermentationofplantcellulosewhichisdifficulttoobtainwithoutharshchemicaltreatments.Fermentationend-productswererelatedtokinetics,withslowcarbohydratefermentationresultinginincreasedproteinfermentation.Ratiosofshort-chainfattyacidproductsweresimilarforallarabinoxylanandβ-glucansubstrates.
CharacterizationandpH-dependentsubstratespecificityofalkalophilicxylanasefromBacillusalcalophilus.
Lee,D.S.,Lee,K.H.,Cho,E.J.,Kim,H.M.,Kim,C.S.&Bae,H.J.(2012).JournalofIndustrialMicrobiology&Biotechnology,39(10),1465-1475.
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Thegeneofendo-beta-1-4xylanase,xynT,wasclonedfromBacillusalcalophilusAX2000andexpressedinEscherichiacoli.ThisXynT,whichbelongstoglycosidehydrolase(GH)family10,wasfoundtohaveamolecularweightofapproximately37kDaandexhibitoptimalactivityatpH7–9and50°C.Itexhibitsahighactivitytowardsbirchwoodxylanandhastheabilitytobindavicel.Underoptimalconditions,XynThydrolyzesallxylooligomersintoxylobioseasanendproductwithapreferenceforcleavagesitesatthesecondorthirdglycosidicbondfromthereducingend.XynThasadifferentsubstrateaffinityonxylooligomersatpH5.0,whichcontributestoitslowactivitytowardxylotrioseanditsderivedintermediateproducts.Thislowactivitymaybeduetoanunstableinteractionwiththeaminoacidsthatconstitutesubsitesoftheactivesite.Interestingly,theadditionofCo2+andMn2+ledtoasignificantincreaseinactivitybyupto40and50%,respectively.XynTpossessesahighbindingaffinityandhydrolyticactivitytowardtheinsolublexylan,forwhichitexhibitshighactivityatpH7–9,givingrisetoitsefficientbiobleachingeffectonPinusdensiflorakraftpulp.
品牌介绍
Megazyme品牌产品简介
来源：作者：人气：2149发表时间：2016-05-19 10:59:00【大 中 小】
Megazyme是一家全球性公司，专注于开发和提供用于饮料、谷物、乳制品、食品、饲料、发酵、生物燃料和葡萄酒产业用的分析试剂、酶和检测试剂盒。Megazyme的许多检测试剂盒产品已经为众多官方科学协会（包括AOAC, AACC , RACI, EBC和ICC等），经过严格的审核，批准认证为官方标准方法，确保以准确、可靠、定量和易于使用的测试方法，满足客户的质量诉求。
Megazyme的主要产品线包括：
◆ 检测试剂盒
◆ 酶
◆ 酶底物
◆ 碳水化合物
◆ 化学品/仪器
官网地址：http://www.megazyme.com
检测试剂盒特色产品：
货号
中文品名
用途
K-ACETAF
乙酸[AF法]检测试剂盒
酶法定量分析乙酸最广泛使用的方法
K-ACHDF
可吸收糖/膳食纤维检测试剂盒
酒精沉淀法测定膳食纤维
K-AMIAR
氨快速检测试剂盒
用于包括葡萄汁、葡萄酒以及其它食品饮料样品中氨含量的快速检测分析。
K-AMYL
直链淀粉/支链淀粉检测试剂盒
谷物淀粉和而粉中直链淀粉/支链淀粉比例和含量检测
K-ARAB
阿拉伯聚糖检测试剂盒
果汁浓缩液中阿拉伯聚糖的检测
K-ASNAM
L-天冬酰胺/L-谷氨酰胺和氨快速检测试剂盒
用于食品工业中丙烯酰胺前体、细胞培养基、以及上清液组分中、L-天冬酰胺，谷氨酰胺和氨的检测分析
K-ASPTM
阿斯巴甜检测试剂盒
专业用于测定饮料和食品中阿斯巴甜含量，操作简单
K-BETA3
β-淀粉酶检测试剂盒
适用于麦芽粉中β-淀粉酶的测定
K-BGLU
混合键β-葡聚糖检测试剂盒
测定谷物、荞麦粉、麦汁、啤酒及其它食品中混合键β-葡聚糖(1,3:1,4-β-D-葡聚糖)的含量
K-CERA
α-淀粉酶检测试剂盒
谷物和发酵液（真菌和细菌）中α-淀粉酶的分析测定
K-CITR
柠檬酸检测试剂盒
快速、可靠地检测食品、饮料和其它物料中柠檬酸（柠檬酸盐）含量
K-DLATE
乳酸快速检测试剂盒
快速、特异性检测饮料、肉类、奶制品和其它食品中L-乳酸和D-乳酸(乳酸盐）含量
K-EBHLG
酵母β-葡聚糖酶检测试剂盒
用于测量和分析酵母中1,3:1,6?-β-葡聚糖，也可以检测1,3-葡聚糖
K-ETSULPH
总亚硫酸检测试剂盒
测定葡萄酒、饮料、食品和其他物料中总亚硫酸含量（按二氧化硫计）的一种简单，高效，可靠的酶法检测方法
K-FRGLMQ
D-果糖/D-葡萄糖[MegaQuant法]检测试剂盒
适用于使用megaquant?色度计（505nm下）测定葡萄、葡萄汁和葡萄酒中D-果糖和D-葡萄糖的含量。
K-FRUC
果聚糖检测试剂盒
含有淀粉、蔗糖和其他糖类的植物提取物和食品中果聚糖的含量测定。
K-FRUGL
D-果糖/D-葡萄糖检测试剂盒
对植物和食品中果糖或葡萄糖含量的酶法紫外分光测定。
K-GALM
半乳甘露聚糖检测试剂盒
食品和植物产品中半乳甘露聚糖的含量检测
K-GLUC
D-葡萄糖[GOPOD]检测试剂盒
谷物提取物中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUHK
D-葡萄糖[HK]检测试剂盒
植物和食品中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUM
葡甘聚糖检测试剂盒
植物和食品中葡甘聚糖的含量测定。
K-INTDF
总膳食纤维检测试剂盒
总膳食纤维特定检测和分析
K-LACGAR
乳糖/D-半乳糖快速检测试剂盒
用于快速检测食品和植物产品中乳糖、D-半乳糖和L-阿拉伯糖
K-LACSU
乳糖/蔗糖/D-葡萄糖检测试剂盒
混合面粉和其它物料中蔗糖、乳糖和D-葡萄糖的测定
K-LACTUL
乳果糖检测试剂盒
特异性、快速和灵敏测量奶基样品中乳果糖含量
K-MANGL
D-甘露糖/D-果糖/D-葡萄糖检测试剂盒
适合测定植物产品和多糖酸性水解产物中D-甘露糖含量
K-MASUG
麦芽糖/蔗糖/D-葡萄糖检测试剂盒
在植物和食品中麦芽糖，蔗糖和葡萄糖的含量检测
K-PECID
胶质识别检测试剂盒
食品配料中果胶的鉴别
K-PHYT
植酸(总磷)检测试剂盒
食品和饲料样品植酸/总磷含量测量的简便方法。不需要通过阴离子交换色谱对植酸纯化，适合于大量样本分析
K-PYRUV
丙酮酸检测试剂盒
在啤酒、葡萄酒、果汁、食品和体液中丙酮酸分析
K-RAFGA
棉子糖/D-半乳糖检测试剂盒
快速测量植物材料和食品中棉子糖和半乳糖含量
K-RAFGL
棉子糖/蔗糖/D-半乳糖检测试剂盒
分析种子和种子粉中D-葡萄糖、蔗糖、棉子糖、水苏糖和毛蕊花糖含量。通过将棉子糖、水苏糖和毛蕊花糖酶解D-葡萄糖、D-果糖和半乳糖，从而测定葡萄糖含量来确定
K-SDAM
淀粉损伤检测试剂盒
谷物面粉中淀粉损伤的检测和分析
K-SUCGL
蔗糖/D-葡萄糖检测试剂盒
饮料、果汁、蜂蜜和食品中蔗糖和葡萄糖的分析
K-SUFRG
蔗糖/D-果糖/D-葡萄糖检测试剂盒
适用于植物和食品中蔗糖、D-葡萄糖和D-果糖的测定
K-TDFR
总膳食纤维检测试剂盒
总膳食纤维检测
K-TREH
海藻糖检测试剂盒
快速、可靠地检测食品、饮料和其它物料中海藻糖含量
K-URAMR
尿素/氨快速检测试剂盒
适用于水、饮料、乳制品和食品中尿素和氨的快速测定
K-URONIC
D-葡萄糖醛酸/D-半乳糖醛酸检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中六元糖醛酸含量（D-葡萄糖醛酸和D-半乳糖醛酸）
K-XYLOSE
D-木糖检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中D-木糖含量
K-YBGL
Beta葡聚糖[酵母和蘑菇]检测试剂盒
检测酵母和蘑菇制品中1,3:1,6-beta-葡聚糖和α-葡聚糖含量
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