Megazyme/β-Glucan (Barley; Low Viscosity)/P-BGBL/5 grams
商品编号：
P-BGBL
品牌：
Megazyme INC
市场价：
¥5904.00
美元价：
3542.40
产品分类：
其他试剂
公司分类：
Other_reagents
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Highpurityβ-Glucan(Barley;LowViscosity)foruseinresearch,biochemicalenzymeassaysandinvitrodiagnosticanalysis.
Purity~95%.Lowviscosityβ-Glucanfrombarleyflour.Viscosity~11CST.
Enzymicquantificationof(1→3)(1→4)-β-D-glucaninbarleyandmalt.
McCleary,B.V.&Glennie-Holmes,M.(1985).JournaloftheInstituteofBrewing,91(5),285-295.
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Asimpleandquantitativemethodforthedeterminationof(1→3)(1→4)-β-D-glucaninbarleyflourandmaltisdescribed.Themethodallowsdirectanalysisofβ-glucaninflourandmaltslurries.Mixed-linkageβ-glucanisspecificallydepolymerizedwithahighlypurified(1→3)(1→4)-β-D-glucanase(lichenase),fromBacillussubtilis,totri-,tetra-andhigherdegreeofpolymerization(d.p.)oligosaccharides.Theseoligosaccharidesarethenspecificallyandquantitativelyhydrolysedtoglucoseusingpurifiedβ-D-glucosidase.Theglucoseisthenspecificallydeterminedusingglucoseoxidase/peroxidasereagent.Sincebarleyflourscontainonlylowlevelsofglucose,andmaltosaccharidesdonotinterferewiththeassay,removaloflowd.p.sugarsisnotnecessary.Blankvaluesaredeterminedforeachsampleallowingthedirectmeasurementofβ-glucaninmaltsamples.α-Amylasedoesnotinterferewiththeassay.Themethodissuitablefortheroutineanalysisofβ-glucaninbarleysamplesderivedfrombreedingprograms;50samplescanbeanalysedbyasingleoperatorinaday.Evaluationofthetechniqueondifferentdayshasindicatedameanstandarderrorof0–1forbarleyfloursamplescontaining3–8and4–6%(w/w)β-glucancontent.
Measurementof(1→3)(1→4)-β-D-glucaninmalt,wortandbeer.
McCleary,B.V.&Nurthen,E.(1986).JournaloftheInstituteofBrewing,92(2),168-173.
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Amethoddevelopedforthequantificationof(1→3)(1→4)-β-D-glucaninbarleyflourhasbeenmodifiedtoallowitsuseinthemeasurementofthiscomponentinmalt,wort,beerandspentgrain.Formaltsamples,freeD-glucosewasfirstremovedwithaqueousethanol.Quantificationofthepolymerinwortandbeersamplesinvolvedprecipitationoftheβ-glucanwithammoniumsulphatefollowedbywashingwithaqueousethanoltoremovefreeD-glucose.Spentgrainwaslyophilisedandmilledandthenanalysedbythemethoddevelopedformalt.Inallcases,theβ-glucanwasdepolymerisedwithlichenaseandtheresultantβ-gluco-oligosaccharideshydrolysedtoD-glucosewithβ-D-glucosidase.ThereleasedD-glucosewasthenspecificallydeterminedusingglucoseoxidase-peroxidasereagent.
Enzymichydrolysisandindustrialimportanceofbarleyβ-glucansandwheatflourpentosans.
McCleary,B.V.,Gibson,T.S.,Allen,H.&Gams,T.C.(1986).Starch-Starke,38(12),433-437.
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Mixedlinkageβ-glucaneandpentosanes(mainlyarABInoxylanes)arethemajorendospermcell-wallpolysaccharidesofbarleyandwheatrespectively.Thesepolysaccharides,althoughminorcomponentsofthewholegrain,significantlyaffecttheindustrialutilizationofthesecereals.Themodificationofbarleycornsduringmaltingrequiresthedissolutionoftheβ-glucaneinthecell-wallofthestarchendosperm.Highβ-glucaneconcentrationinwortandbeereffecttherateoffiltrationandcanalsoleadtoprecipitateorgelformationinthefinalproduct.Inasimilarmanner,pentosaneisthoughttocausefiltrationproblemswithwheatstarchhydrolysatesbyincreasingviscosityandbyproducinggelatinousprecipitatewhichblocksfilters.Ironically,itisthissameviscositybuildingandwaterbindingcapacitywhichisconsideredtorenderpentosanesofconsiderablevalueindoughdevelopmentandbreadstorage(anti-stalingfunctions).Inthecurrentpaper,someaspectsofthebeneficialanddetrimentaleffectsofpentosanesandβ-glucaneintheindustrialutilizationofwheatandbarleyarediscussed.Morespecifically,enzymicmethodsforthepreparation,analysisandidentificationofthesepolysaccharidesandfortheremovaloftheirfunctionalproperties,aredescribedindetail.
Measurementof(1→3),(1→4)-β-D-glucaninbarleyandoats:Astreamlinedenzymicprocedure.
McCleary,B.V.&Codd,R.(1991).JournaloftheScienceofFoodandAgriculture,55(2),303-312.
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Acommerciallyavailableenzymicmethodforthequantitativemeasurementof(1→3),(1→4)-β-glucanhasbeensimplifiedtoallowanalysisofupto10grainsamplesin70minorof100–200samplesbyasingleoperatorinaday.Theseimprovementshavebeenachievedwithnolossinaccuracyorprecisionandwithanincreaseinreliability.Theglucoseoxidase/peroxidasereagenthasbeensignificantlyimprovedtoensurecolourstabilityforperiodsofupto1hafterdevelopment.Someproblemsexperiencedwiththeoriginalmethodhavebeenaddressedandresolved,andfurtherexperimentstodemonstratethequantitativenatureoftheassayhavebeendesignedandperformed.
InVitrofermentationofoatandbarleyderivedβ-glucansbyhumanfaecalmicrobiota.
Hughes,S.A.,Shewry,P.R.,Gibson,G.R.,McCleary,B.V.&Rastall,R.A.(2008).FEMSMicroBIOLOGyEcology,64(3),482–493.
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Fermentationofβ-glucanfractionsfrombarley[averagemolecularmass(MM),of243,172,and137kDa]andoats(averageMMof230and150kDa)bythehumanfaecalmicrobiotawasinvestigated.FractionsweresupplementedtopH-controlledanaerobicbatchculturefermentersinoculatedwithhumanfaecalsamplesfromthreedonors,intriplicate,foreachsubstrate.Microbiotachangesweremonitoredbyfluorescentinsituhybridization;groupsenumeratedwere:Bifidobacteriumgenus,BacteroidesandPrevotellagroup,Clostridiumhistolyticumsubgroup,Ruminococcus-Eubacterium-Clostridium(REC)cluster,Lactobacillus-Enterococcusgroup,Atopobiumcluster,andclostridialclusterIX.Short-chainfattyacidsandlacticacidweremeasuredbyHPLC.TheC.histolyticumsubgroupincreasedsignificantlyinallvesselsandclostridialclusterIXmaintainedhighpopulationswithallfractions.TheBacteroides-Prevotellagroupincreasedwithallbutthe243-kDabarleyand230-kDaoatsubstrates.Ingeneralβ-glucansdisplayednoapparentprebioticpotential.TheSCFAprofile(51:32:17;acetate:propionate:butyrate)wasconsideredpropionate-rich.Inafurtherstudyaβ-glucanoligosaccharidefractionwasproducedwithadegreeofpolymerizationof3-4.Thisfractionwassupplementedtosmall-scalefaecalbatchculturesandgavesignificantincreasesintheLactobacillus-Enterococcusgroup;however,theprebioticpotentialofthisfractionwasmarginalcomparedwiththatofinulin.
CompletegenomeofanewFirmicutesspeciesbelongingtothedominanthumancolonicmicrobiota(‘Ruminococcusbicirculans’)revealstwochromosomesandaselectivecapacitytoutilizeplantglucans.
Wegmann,U.,Louis,P.,Goesmann,A.,Henrissat,B.,Duncan,S.H.&Flint,H.J.(2014).EnvironmentalMicrobiology,16(9),2879–2890.
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Therecentlyisolatedbacterialstrain80/3representsoneofthemostabundant16SrRNAphylotypesdetectedinthehealthyhumanlargeintestineandbelongstotheRuminococcaceaefamilyofFirmicutes.Thecompletedgenomesequencereportedhereisthefirstforamemberofthisimportantfamilyofbacteriafromthehumancolon.Thegenomecomprisestwolargechromosomesof2.24and0.73Mbp,leADIngustoproposethenameRuminococcusbicirculansforthisnewspecies.Analysisofthecarbohydrateactiveenzymecomplementsuggestsanabilitytoutilizecertainhemicelluloses,especiallyβ-glucansandxyloglucan,forgrowththatwasconfirmedexperimentally.Theenzymaticmachineryenablingthedegradationofcelluloseandxylanbyrelatedcellulolyticruminococciishoweverlackinginthisspecies.Whilethegenomeindicatedthecapacitytosynthesizepurines,pyrimidinesandall20aminoacids,onlygenesforthesynthesisofnicotinate,NAD+,NADP+andcoenzymeAweredetectedamongtheessentialvitaminsandco-factors,resultinginmultiplegrowthrequirements.Invivo,thesegrowthfactorsmustbesuppliedfromthediet,hostorothergutmicroorganisms.OtherfeaturesofecologicalinterestincludetwotypeIVpilins,multipleextracytoplasmicfunction-sigmafactors,aureaseandabilesalthydrolase.
Structuralbasisforentropy-drivencellulosebindingbyatype-Acellulose-bindingmodule(CBM)andbacterialexpansin.
Georgelis,N.,Yennawar,N.H.&Cosgrove,D.J.(2012).ProceedingsoftheNationalAcademyofSciences,109(37),14830-14835.
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Componentsofmodularcellulases,type-Acellulose-bindingmodules(CBMs)bindtocrystallinecelluloseandenhanceenzymeeffectiveness,butstructuraldetailsoftheinteractionareuncertain.WeanalyzedcellulosebindingbyEXLX1,abacterialexpansinwithabilitytoloosenplantcellwallsandwhosedomainD2hastype-ACBMcharacteristics.EXLX1stronglybindstocrystallinecelluloseviaD2,whereasitsaffinityforsolublecellooligosaccharidesisweak.Calorimetryindicatedcellulosebindingwaslargelyentropicallydriven.WesolvedthecrystalstructuresofEXLX1complexedwithcellulose-likeoligosaccharidestofindthatEXLX1bindstheligandsthroughhydrophobicinteractionsofthreelinearlyarrangedaromaticresiduesinD2.Thecrystalstructuresrevealedauniqueformofligand-mediateddimerization,withtheoligosaccharidesandwichedbetweentwoD2domainsinoppositepolarity.Thisreportclarifiesthemoleculartargetofexpansinandthespecificmolecularinteractionsofatype-ACBMwithcellulose.
Invitrofermentationkineticsandend-productsofcerealarabinoxylansand(1,3;1,4)-β-glucansbyporcinefaeces.
Williams,B.A.,Mikkelsen,D.,LePaih,L.&Gidley,M.J.(2011).JournalofCerealScience,53(1),53-58.
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Purifiedandsemi-purifiedpolysaccharidescharacteristicofcerealswerefermentedinvitrowithapigfaecalinoculum,usingthecumulativegasproductiontechnique,toexaminethekineticsandend-productsoffermentationafter48h.Itwasshownthatarabinoxylanandmixedlinkage(1,3;1,4)β-glucanwererapidlyfermentedifsoluble,whilelesssolublesubstrates(insolublearabinoxylan,maizeandwheatstarchgranules,andbacterialcellulose)weremoreslowlyfermented.Relevantmonosaccharideswerefermentedatverysimilarratestosolublepolymericarabinoxylanandβ-glucan,showingthatdepolymerisationwasnotalimitingstep,incontrasttosomepreviousstudies.Bacterialcelluloseisshowntobeausefulmodelsubstrateforfermentationofplantcellulosewhichisdifficulttoobtainwithoutharshchemicaltreatments.Fermentationend-productswererelatedtokinetics,withslowcarbohydratefermentationresultinginincreasedproteinfermentation.Ratiosofshort-chainfattyacidproductsweresimilarforallarabinoxylanandβ-glucansubstrates.
DistinctionoffungalpolysaccharidesbyN/Cratioandmidinfraredspectroscopy.
Gomba,G.K.,Synytsya,A.,Švecová,P.,Coimbra,M.A.&Čopíková,J.(2015).InternationalJournalofBiologicalMacromolecules,80,271-281.
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AsetoffungalpolysaccharidesampleswascharacterisedbyelementalanalysisandFTIRspectroscopyandcomparedwithreferencechitins,chitosansandβ-D-glucans.Thenitrogentocarbon(N/C)valuesandFTIRspectrawereusedtocomparethesamplesbasedontheircomposition.ItwasfoundthattheN/Cratiocorrelateswellwithdeacetylationdegree(DD)ofchitosansandchitin/glucanratioRchitoffungalchitin–β-D-glucancomplexeswiththeexceptionofsomesampleshavingsignificantnitrogenand/orcarbonadmixtures.FTIRspectroscopywasindicativefortheN-acetylationofchitins(chitosans)aswellasforthechitin(chitosan)contributiontofungalpolysaccharidepreparations.MultivariateanalysesoftheFTIRdata(HCA,PCA)discriminatedsamplesandreferencematerialsintoseveralclustersdependingontheirsimilarity.Chitosanlactates,chitosan–β-D-glucansandchitin–β-D-glucansofhighandlowamountsofchitinweresuccessfullydiscriminatedfromthereferencepolysaccharidesandfromeachother.TheproposedproceduresbasedontheN/CratioandmultivariateanalysesofFTIRspectramaybeusedinscreeningfungalpolysaccharidepreparations.
Effectsofinvitrofermentationofbarleyβ‐glucanandsugarbeetpectinusinghumanfecalinoculaoncytokineexpressionbydendriticcells.
Rösch,C.,Taverne,N.,Venema,K.,Gruppen,H.,Wells,J.M.&Schols,H.A.(2017).Molecularnutrition&FoodResearch,61(1).
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Scope:Thisstudysimulatesthefermentationprocessofbarleyβ-glucanandsugarbeetpectininthehumancolonandmonitorsthedegradationproductsformed.Additionally,immuneeffectsofthedegradationproductswereinvestigated.Methodsandresults:Immunostimulatoryactivityoffermentationdigestawasinvestigatedusingbonemarrowderiveddendriticcells(BMDCs)fromtoll-likereceptor2/4(TLR2/4)knockoutmice,whichwereunresponsivetomicrobe-associatedmolecularpatterns.CytokineresponseswereelicitedtodietaryfibersandnottotheSCFAandmicrobiota.ThefermentationdigestawereanalyzedfortheirSCFAprofilesandglycanmetabolitesovertime.DuringfermentationtheamountofinsolubleprecipitatingfibersincreasedandinducedaswellassolublemoleculesoflowermolecularmassgreateramountsofcytokinesinBMDCsthantheparentalfiber.Additionally,highamountsofcytokinescanbeattributedtosolublegalactose-richbeetpectinmolecules.Conclusions:Thefermentationofthetwofibersledtofiber-specificamountsofSCFA,glycosidicmetabolites,anddifferentimmunomodulatoryproperties.BMDCfromTLR2/4knockoutmicedidnotrespondtothedigestmicrobiotaandSCFA,makingitausefulapproachtostudytemporaleffectsoffermentationontheimmunomodulatoryeffectsoffibers.
Invitrofermentationofbeta-glucansandotherselectedcarbohydratesbyinfantfecalinoculum:Anevaluationoftheirpotentialasprebioticsininfantformula.
Lam,K.L.,Keung,H.Y.,Ko,K.C.,Kwan,H.S.&Cheung,P.C.K.(2017).BioactiveCarbohydratesandDietaryFibre,InPress.
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Prebioticsarebeingaddedtoinfantformulainordertomimictheeffectsofhumanmilkoligosaccharides(HMOs)forthegrowthofprobioticbacteriaespeciallybifidobacteriaandlactobacilliintheinfantgut.Thispreliminarystudycomparestheinvitrofermentationof13differentcarbohydratesincludingmonosaccharides,disaccharides,oligosaccharidesandpolysaccharidesbyinfantfecalsamplescollectedfrom3-montholdbreast-milkfedbabies.Thegrowthofthetotalanaerobicbacteriaandtwoprobioticbacteria(bifidobacteriaandlactobacilli)duringthefermentationperiodwasmeasuredbytotalplatecount(TPC)andwasexpressedascolonyformingunits(CFUs).Amongotherthings,beta-glucansseemtoselectivelyenhancethegrowthoflactobacilliforalongerperiodoffermentationtimethanmostofthecarbohydratestested.Theselectiveenrichmentoftheprobioticbacteriabythesecarbohydratesandtheirpotentialuseasprebioticsintheinfantformulaarediscussed.
Isbeerasourceofprebiotics?.
Kanyer,A.J.,Bornhorst,G.M.,Marco,M.L.&Bamforth,C.W.(2017).JournaloftheInstituteofBrewing,123(3),361-365.
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Beercontainslow-molecular-weightβ-linkedoligosaccharidesthatoriginatefromthedegradationofβ-glucaninthebarleycellwallduringmaltingandmashing.Over90%oftheseoligosaccharidescontainthreeorfourglucosylunits.Theyremainintactthroughastaticoral,gastricandsmallintestinalinvitrohumandigestivesystemmodel,indicatingthattheyshouldbeavailabletobeneficialorganismsknowntobepresentinthehumanlargeintestine.Severalintestine-associatedLactobacillusstrainswereshowntobecapableofgrowthontheseβ-linkedoligosaccharides,therebyleadingustotentativelyproposethatthesecompoundsmayrepresentprebiotics.
品牌介绍
Megazyme品牌产品简介
来源：作者：人气：2149发表时间：2016-05-19 10:59:00【大 中 小】
Megazyme是一家全球性公司，专注于开发和提供用于饮料、谷物、乳制品、食品、饲料、发酵、生物燃料和葡萄酒产业用的分析试剂、酶和检测试剂盒。Megazyme的许多检测试剂盒产品已经为众多官方科学协会（包括AOAC, AACC , RACI, EBC和ICC等），经过严格的审核，批准认证为官方标准方法，确保以准确、可靠、定量和易于使用的测试方法，满足客户的质量诉求。
Megazyme的主要产品线包括：
◆ 检测试剂盒
◆ 酶
◆ 酶底物
◆ 碳水化合物
◆ 化学品/仪器
官网地址：http://www.megazyme.com
检测试剂盒特色产品：
货号
中文品名
用途
K-ACETAF
乙酸[AF法]检测试剂盒
酶法定量分析乙酸最广泛使用的方法
K-ACHDF
可吸收糖/膳食纤维检测试剂盒
酒精沉淀法测定膳食纤维
K-AMIAR
氨快速检测试剂盒
用于包括葡萄汁、葡萄酒以及其它食品饮料样品中氨含量的快速检测分析。
K-AMYL
直链淀粉/支链淀粉检测试剂盒
谷物淀粉和而粉中直链淀粉/支链淀粉比例和含量检测
K-ARAB
阿拉伯聚糖检测试剂盒
果汁浓缩液中阿拉伯聚糖的检测
K-ASNAM
L-天冬酰胺/L-谷氨酰胺和氨快速检测试剂盒
用于食品工业中丙烯酰胺前体、细胞培养基、以及上清液组分中、L-天冬酰胺，谷氨酰胺和氨的检测分析
K-ASPTM
阿斯巴甜检测试剂盒
专业用于测定饮料和食品中阿斯巴甜含量，操作简单
K-BETA3
β-淀粉酶检测试剂盒
适用于麦芽粉中β-淀粉酶的测定
K-BGLU
混合键β-葡聚糖检测试剂盒
测定谷物、荞麦粉、麦汁、啤酒及其它食品中混合键β-葡聚糖(1,3:1,4-β-D-葡聚糖)的含量
K-CERA
α-淀粉酶检测试剂盒
谷物和发酵液（真菌和细菌）中α-淀粉酶的分析测定
K-CITR
柠檬酸检测试剂盒
快速、可靠地检测食品、饮料和其它物料中柠檬酸（柠檬酸盐）含量
K-DLATE
乳酸快速检测试剂盒
快速、特异性检测饮料、肉类、奶制品和其它食品中L-乳酸和D-乳酸(乳酸盐）含量
K-EBHLG
酵母β-葡聚糖酶检测试剂盒
用于测量和分析酵母中1,3:1,6?-β-葡聚糖，也可以检测1,3-葡聚糖
K-ETSULPH
总亚硫酸检测试剂盒
测定葡萄酒、饮料、食品和其他物料中总亚硫酸含量（按二氧化硫计）的一种简单，高效，可靠的酶法检测方法
K-FRGLMQ
D-果糖/D-葡萄糖[MegaQuant法]检测试剂盒
适用于使用megaquant?色度计（505nm下）测定葡萄、葡萄汁和葡萄酒中D-果糖和D-葡萄糖的含量。
K-FRUC
果聚糖检测试剂盒
含有淀粉、蔗糖和其他糖类的植物提取物和食品中果聚糖的含量测定。
K-FRUGL
D-果糖/D-葡萄糖检测试剂盒
对植物和食品中果糖或葡萄糖含量的酶法紫外分光测定。
K-GALM
半乳甘露聚糖检测试剂盒
食品和植物产品中半乳甘露聚糖的含量检测
K-GLUC
D-葡萄糖[GOPOD]检测试剂盒
谷物提取物中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUHK
D-葡萄糖[HK]检测试剂盒
植物和食品中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUM
葡甘聚糖检测试剂盒
植物和食品中葡甘聚糖的含量测定。
K-INTDF
总膳食纤维检测试剂盒
总膳食纤维特定检测和分析
K-LACGAR
乳糖/D-半乳糖快速检测试剂盒
用于快速检测食品和植物产品中乳糖、D-半乳糖和L-阿拉伯糖
K-LACSU
乳糖/蔗糖/D-葡萄糖检测试剂盒
混合面粉和其它物料中蔗糖、乳糖和D-葡萄糖的测定
K-LACTUL
乳果糖检测试剂盒
特异性、快速和灵敏测量奶基样品中乳果糖含量
K-MANGL
D-甘露糖/D-果糖/D-葡萄糖检测试剂盒
适合测定植物产品和多糖酸性水解产物中D-甘露糖含量
K-MASUG
麦芽糖/蔗糖/D-葡萄糖检测试剂盒
在植物和食品中麦芽糖，蔗糖和葡萄糖的含量检测
K-PECID
胶质识别检测试剂盒
食品配料中果胶的鉴别
K-PHYT
植酸(总磷)检测试剂盒
食品和饲料样品植酸/总磷含量测量的简便方法。不需要通过阴离子交换色谱对植酸纯化，适合于大量样本分析
K-PYRUV
丙酮酸检测试剂盒
在啤酒、葡萄酒、果汁、食品和体液中丙酮酸分析
K-RAFGA
棉子糖/D-半乳糖检测试剂盒
快速测量植物材料和食品中棉子糖和半乳糖含量
K-RAFGL
棉子糖/蔗糖/D-半乳糖检测试剂盒
分析种子和种子粉中D-葡萄糖、蔗糖、棉子糖、水苏糖和毛蕊花糖含量。通过将棉子糖、水苏糖和毛蕊花糖酶解D-葡萄糖、D-果糖和半乳糖，从而测定葡萄糖含量来确定
K-SDAM
淀粉损伤检测试剂盒
谷物面粉中淀粉损伤的检测和分析
K-SUCGL
蔗糖/D-葡萄糖检测试剂盒
饮料、果汁、蜂蜜和食品中蔗糖和葡萄糖的分析
K-SUFRG
蔗糖/D-果糖/D-葡萄糖检测试剂盒
适用于植物和食品中蔗糖、D-葡萄糖和D-果糖的测定
K-TDFR
总膳食纤维检测试剂盒
总膳食纤维检测
K-TREH
海藻糖检测试剂盒
快速、可靠地检测食品、饮料和其它物料中海藻糖含量
K-URAMR
尿素/氨快速检测试剂盒
适用于水、饮料、乳制品和食品中尿素和氨的快速测定
K-URONIC
D-葡萄糖醛酸/D-半乳糖醛酸检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中六元糖醛酸含量（D-葡萄糖醛酸和D-半乳糖醛酸）
K-XYLOSE
D-木糖检测试剂盒
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