Megazyme/&beta-葡聚糖(大麦;中等粘度)/P-BGBM/5克
商品编号:
P-BGBM
品牌:
Megazyme INC
市场价:
¥6168.00
美元价:
3700.80
产品分类:
其他试剂
公司分类:
Other_reagents
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
HighpurityBeta-Glucan(Barley;MediumViscosity)foruseinresearch,biochemicalenzymeassaysandinvitrodiagnosticanalysis.
Purity~95%.Mediumviscosityβ-glucanfrombarleyflour.Fortheassayofmaltβ-glucanaseandcellulases.SuitableforInstituteofBrewingviscometricassay.Viscosity20-30CST.
Enzymicquantificationof(1→3)(1→4)-β-D-glucaninbarleyandmalt.
McCleary,B.V.&Glennie-Holmes,M.(1985).JournaloftheInstituteofBrewing,91(5),285-295.
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Asimpleandquantitativemethodforthedeterminationof(1→3)(1→4)-β-D-glucaninbarleyflourandmaltisdescribed.Themethodallowsdirectanalysisofβ-glucaninflourandmaltslurries.Mixed-linkageβ-glucanisspecificallydepolymerizedwithahighlypurified(1→3)(1→4)-β-D-glucanase(lichenase),fromBacillussubtilis,totri-,tetra-andhigherdegreeofpolymerization(d.p.)oligosaccharides.Theseoligosaccharidesarethenspecificallyandquantitativelyhydrolysedtoglucoseusingpurifiedβ-D-glucosidase.Theglucoseisthenspecificallydeterminedusingglucoseoxidase/peroxidasereagent.Sincebarleyflourscontainonlylowlevelsofglucose,andmaltosaccharidesdonotinterferewiththeassay,removaloflowd.p.sugarsisnotnecessary.Blankvaluesaredeterminedforeachsampleallowingthedirectmeasurementofβ-glucaninmaltsamples.α-Amylasedoesnotinterferewiththeassay.Themethodissuitablefortheroutineanalysisofβ-glucaninbarleysamplesderivedfrombreedingprograms;50samplescanbeanalysedbyasingleoperatorinaday.Evaluationofthetechniqueondifferentdayshasindicatedameanstandarderrorof0–1forbarleyfloursamplescontaining3–8and4–6%(w/w)β-glucancontent.
Measurementof(1→3)(1→4)-β-D-glucaninmalt,wortandbeer.
McCleary,B.V.&Nurthen,E.(1986).JournaloftheInstituteofBrewing,92(2),168-173.
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Amethoddevelopedforthequantificationof(1→3)(1→4)-β-D-glucaninbarleyflourhasbeenmodifiedtoallowitsuseinthemeasurementofthiscomponentinmalt,wort,beerandspentgrain.Formaltsamples,freeD-glucosewasfirstremovedwithaqueousethanol.Quantificationofthepolymerinwortandbeersamplesinvolvedprecipitationoftheβ-glucanwithammoniumsulphatefollowedbywashingwithaqueousethanoltoremovefreeD-glucose.Spentgrainwaslyophilisedandmilledandthenanalysedbythemethoddevelopedformalt.Inallcases,theβ-glucanwasdepolymerisedwithlichenaseandtheresultantβ-gluco-oligosaccharideshydrolysedtoD-glucosewithβ-D-glucosidase.ThereleasedD-glucosewasthenspecificallydeterminedusingglucoseoxidase-peroxidasereagent.
Enzymichydrolysisandindustrialimportanceofbarleyβ-glucansandwheatflourpentosans.
McCleary,B.V.,Gibson,T.S.,Allen,H.&Gams,T.C.(1986).Starch-Starke,38(12),433-437.
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Mixedlinkageβ-glucaneandpentosanes(mainlyarABInoxylanes)arethemajorendospermcell-wallpolysaccharidesofbarleyandwheatrespectively.Thesepolysaccharides,althoughminorcomponentsofthewholegrain,significantlyaffecttheindustrialutilizationofthesecereals.Themodificationofbarleycornsduringmaltingrequiresthedissolutionoftheβ-glucaneinthecell-wallofthestarchendosperm.Highβ-glucaneconcentrationinwortandbeereffecttherateoffiltrationandcanalsoleadtoprecipitateorgelformationinthefinalproduct.Inasimilarmanner,pentosaneisthoughttocausefiltrationproblemswithwheatstarchhydrolysatesbyincreasingviscosityandbyproducinggelatinousprecipitatewhichblocksfilters.Ironically,itisthissameviscositybuildingandwaterbindingcapacitywhichisconsideredtorenderpentosanesofconsiderablevalueindoughdevelopmentandbreadstorage(anti-stalingfunctions).Inthecurrentpaper,someaspectsofthebeneficialanddetrimentaleffectsofpentosanesandβ-glucaneintheindustrialutilizationofwheatandbarleyarediscussed.Morespecifically,enzymicmethodsforthepreparation,analysisandidentificationofthesepolysaccharidesandfortheremovaloftheirfunctionalproperties,aredescribedindetail.
Measurementof(1→3),(1→4)-β-D-glucaninbarleyandoats:Astreamlinedenzymicprocedure.
McCleary,B.V.&Codd,R.(1991).JournaloftheScienceofFoodandAgriculture,55(2),303-312.
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Acommerciallyavailableenzymicmethodforthequantitativemeasurementof(1→3),(1→4)-β-glucanhasbeensimplifiedtoallowanalysisofupto10grainsamplesin70minorof100–200samplesbyasingleoperatorinaday.Theseimprovementshavebeenachievedwithnolossinaccuracyorprecisionandwithanincreaseinreliability.Theglucoseoxidase/peroxidasereagenthasbeensignificantlyimprovedtoensurecolourstabilityforperiodsofupto1hafterdevelopment.Someproblemsexperiencedwiththeoriginalmethodhavebeenaddressedandresolved,andfurtherexperimentstodemonstratethequantitativenatureoftheassayhavebeendesignedandperformed.
InVitrofermentationofoatandbarleyderivedβ-glucansbyhumanfaecalmicrobiota.
Hughes,S.A.,Shewry,P.R.,Gibson,G.R.,McCleary,B.V.&Rastall,R.A.(2008).FEMSMicroBIOLOGyEcology,64(3),482–493.
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Fermentationofβ-glucanfractionsfrombarley[averagemolecularmass(MM),of243,172,and137kDa]andoats(averageMMof230and150kDa)bythehumanfaecalmicrobiotawasinvestigated.FractionsweresupplementedtopH-controlledanaerobicbatchculturefermentersinoculatedwithhumanfaecalsamplesfromthreedonors,intriplicate,foreachsubstrate.Microbiotachangesweremonitoredbyfluorescentinsituhybridization;groupsenumeratedwere:Bifidobacteriumgenus,BacteroidesandPrevotellagroup,Clostridiumhistolyticumsubgroup,Ruminococcus-Eubacterium-Clostridium(REC)cluster,Lactobacillus-Enterococcusgroup,Atopobiumcluster,andclostridialclusterIX.Short-chainfattyacidsandlacticacidweremeasuredbyHPLC.TheC.histolyticumsubgroupincreasedsignificantlyinallvesselsandclostridialclusterIXmaintainedhighpopulationswithallfractions.TheBacteroides-Prevotellagroupincreasedwithallbutthe243-kDabarleyand230-kDaoatsubstrates.Ingeneralβ-glucansdisplayednoapparentprebioticpotential.TheSCFAprofile(51:32:17;acetate:propionate:butyrate)wasconsideredpropionate-rich.Inafurtherstudyaβ-glucanoligosaccharidefractionwasproducedwithadegreeofpolymerizationof3-4.Thisfractionwassupplementedtosmall-scalefaecalbatchculturesandgavesignificantincreasesintheLactobacillus-Enterococcusgroup;however,theprebioticpotentialofthisfractionwasmarginalcomparedwiththatofinulin.
Preparationofarabinoxylobiosefromryexylanusingfamily10Aspergillusaculeatusendo-1,4-β-D-xylanase.
Rantanen,H.,Virkki,L.,Tuomainen,P.,Kabel,M.,Schols,H.&Tenkanen,M.(2007).CarbohydratePolymers,68(2),350-359.
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CommercialxylanasepreparationShearzyme®,whichcontainstheglycosidehydrolasefamily10endo-1,4-β-D-xylanasefromAspergillusaculeatus,wasusedtoprepareshort-chainarabinoxylo-oligosaccharides(AXOS)fromryearabinoxylan(AX).AmajorAXOSwasformedasahydrolysisproduct.LongerAXOSwerealsoproducedasminorproducts.ThepureGH10xylanasefromA.aculeatuswasusedasacomparisontoensurethattheformedAXOSwereconsequenceoftheendoxylanase‘sfunctioninsteadofsomesideenzymespresentinShearzyme.ThemajorAXOSwaspurifiedandthestructureconfirmedwithvariousanalysismethods(TLC,HPAEC-PAD,MALDI-TOF-MS,andone-andtwo-dimensionalNMRspectroscopywithnano-probe)asα-L-Araf-(1→3)-β-D-Xylp-(1→4)-D-Xylp(arabinoxylobiose).Thisisthefirstreporton13CNMRdataofpurearabinoxylobiose.Theyieldofarabinoxylobiosewas12%fromthequantifiedhydrolysisproducts.Inconclusion,GH10endoxylanasefromA.aculeatusisthusabletocutefficientlythexylosidiclinkagenexttothearabinofuranosyl-substitutedxyloseunitwhichisnottypicalforalltheGH10endoxylanases.Interestingly,pureA.aculeatusxylanaseshowednotablyactivitytowardsp-nitrophenyl-β-Dxylopyranose.InpreviouslystudieslongerAXOShavebeenproducedwithShearzymebuttheformationofshort-chainAXOSbyA.aculeatusGH10xylanasehasnotbeenstudiedbefore.
Newenzyme-basedmethodforanalysisofwater-solublewheatarabinoxylans.
Virkki,L.,Maina,H.N.,Johansson,L.&Tenkanen,M.(2008).CarbohydrateResearch,343(3),521-529.
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Arabinoxylans(AX)arethepredominantcell-wallpolysaccharidesinwheatflour.Water-extractableAXareessentialfordoughandbreadpropertiesandperformance.However,thereisnospecificandaccuratewayofdeterminingthecontentandstructureofAX.Anenzyme-assistedmethodemployinganefficientenzymemixtureforthetotalhydrolysisofAXwasdevelopedinthepresentwork.Enzymatichydrolysis(EH)isagentlemethodduringwhichnounwantedsugardestructionoccurs.FollowingEH,liberatedmonosaccharideswereanalysedbygaschromatography(GC)andliquidchromatographyusingHPAEC–PAD.Theresultswerecomparedwithacidmethanolysis(AM)andacidhydrolysis(AH).EHperformedbetteroncommerciallyisolatedAXsamplesthanthereferencemethodAM.ItsactioninthewaterextractfromwheatflourwasalsomoreefficientthanthatofAMandcomparabletotheefficiencyofAH.HPAEC–PADrevealedasignificantamountoffructoseinthewaterextractfollowingEH,originatingfromfructansinwheatflournotdetectedintheGCanalysis.Thewheatflourexaminedcontained0.29%water-extractableAX.Thearabinose/xyloseratiowas0.32.Theenzyme-basedmethoddevelopedisapplicableforcomparisonofdifferentwheatfloursandcanbeusedinevaluatingtheeffectofprocessingonthecontentandstructureofwater-extractableAX.
WaterextractofTriticumaestivumL.anditscomponentsdemonstrateprotectiveeffectinamodelofvasculardementia.
Han,H.S.,Jang,J.H.,Jang,J.H.,Choi,J.S.,Kim,Y.J.,Lee,C.,Lim,S.H.,Lee,H.K.&Lee,J.(2010).JournalofMedicinalFood,13(3),572-578.
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AlthoughvasculardementiaisthesecondleADIngcauseofdementiaandoftenunderdiagnosed,therearenodrugsyetapprovedforthetreatmentofvasculardementia.Inthisstudy,itisdemonstratedthatwaterextractofTriticumaestivumL.(TALE)andsomeofitscomponentshaveprotectiveeffectsagainstvasculardementia-induceddamagebypreservingthemyelinsheathandinhibitingastrocyticactivation.Thememorytestusedavasculardementiamodelutilizingbilateralligationofthecarotidarteriesofrats.TALE,someofitscomponents,suchasstarch,totaldietaryfiber(TDF),arabinoxylan,β-glucan,anddegradedproductsofarabinoxylan,suchasarabinoseandxylose,wereadmiNISTeredtotheanimalsfromday8today14,followingthesurgery.Twenty-onedaysafterthesurgery,thewatermazetestwasperformedfor5days,andthetimetakentofindtheplatformduringtrainingtrials(meanescapelatency)wasmeasured.ThemeanescapelatencywasdecreasedconsistentlyintheTALE-,starch-,TDF-,arabinoxylan-,andarabinose-treatedgroups,comparedwiththatinthevasculardementiagroup.Tomeasurebraindamage,Luxolfastbluestainingandimmunohistochemistryofmyelinbasicprotein(MBP)wereperformedtoobservemyelinsheathinthewhitematter,andimmunohistochemistryofglialfibrillaryacidicprotein(GFAP)wasperformedtoobservetheastrocyticreaction.VasculardementiareducedtheMBPlevelandincreasedtheGFAPlevel.ArabinoseeffectivelyinhibitedtheMBPandGFAPchange,whereasarabinoxylaninhibitedtheGFAPchangeonly.TheseresultssuggestthatTALEandsomeofitscomponentscanbeusedasamedicinalmaterialforthedevelopmentofneuroprotectiveagentsagainstvasculardementia.
Yeastsinanindustrialmaltingecosystem.
Laitila,A.,Wilhelmson,A.,Kotaviita,E.,Olkku,J.,Home,S.&Juvonen,R.(2006).JournalofIndustrialMicrobiologyandBiotechnology,33(11),953-966.
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Themaltingecosystemconsistsoftwocomponents:thegerminatingcerealgrainsandthecomplexmicrobialcommunity.Yeastsandyeast-likefungiareanimportantpartofthisecosystem,butthecompositionandtheeffectsofthismicrobialgrouphavebeenlargelyunknown.Inthisstudywesurveyedthedevelopmentofyeastsandyeast-likefungiinfourindustrialscalemaltingprocesses.Atotalof136maltingprocesssampleswerecollectedandexaminedforthepresenceofyeastsgrowingat15,25and37°C.Morethan700colonieswereisolatedandcharacterized.TheisolateswerediscriminatedbyPCR-fingerprintingwithmicrosatelliteprimer(M13).YeastsrepresentingdifferentfingerprinttypeswereidentifiedbysequenceanalysisoftheD1/D2domainofthe26SrRNAgene.FurThermore,identifiedyeastswerescreenedfortheproductionofα-amylase,β-glucanase,cellulaseandxylanase.Anumerousanddiverseyeastcommunityconsistingofbothascomycetous(25)andbasidiomycetous(18)specieswasdetectedinthevariousstagesofthemaltingprocess.ThemostfrequentlyisolatedascomycetousyeastsbelongedtothegeneraCandida,Clavispora,Galactomyces,Hanseniaspora,Issatchenkia,Pichia,SaccharomycesandWilliopsisandthebasidiomycetousyeaststoBulleromyces,Filobasidium,Cryptococcus,Rhodotorula,SporobolomycesandTrichosporon.Inaddition,twoascomycetousyeast-likefungi(blackyeasts)belongingtothegeneraAureobasidiumandExophialawerecommonlydetected.Yeastsandyeast-likefungiproducedextracellularhydrolyticenzymeswithapotentiallypositivecontributiontothemaltenzymespectrum.Knowledgeofthemicrobialdiversityprovidesabasisformicrofloramanagementandunderstandingoftheroleofmicrobesinthecerealgerminationprocess.
DifferentialadjuvanteffectsofsolublebetaglucansfrombarleyandSaccharomychescerevisiainprimaryandsecondaryhumoralimmuneresponses.
Uslu,K.&Bagriacik,E.U.(2011).InternationalJournalofHematology&Oncology/UHOD:UluslararasiHematolojiOnkolojiDergisi,21(3).
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Thepurposeofthisstudywastoinvestigateandcompareadjuvanteffectsofsolubleβ-glucansfrombarleyandSaccharomychescerevisiaininductionofantigenspecifichumoralimmuneresponses.Micewereimmunizedwithconalbuminatarelativelylowconcentrationinthepresenceofbetaglucans.Anti-conalbuminantibodiesintheseraofimmunizedandcontrolmicewerequantifiedbyELISA.Athighdoses,bothofglucansincreasedeffectivelylevelsofcirculatingIgMandIgGantibodieswhichwerespecificforconalbumin.However,coadministrationofglucanfrombarleyat1μgdoseresultedinloweryieldinIgG1,IgG2a,IgG2b,andIgAlevelsincomparisontothatofyeast-derivedglucanatthesamedose.Wealsofoundthatantigen(conalbumin)specificantibodylevelsenhancedbyβ-glucanfromSaccharomychescerevisiawerealwayshigherthanthoseoftheglucanfrombarley.Basedonthesefindings,weconcludedthat(1,3),(1,6)-β-D-glucanfromyeastcellwallmighthavesuperiorimmunostimulantactivityininductionofantigenspecifichumoralimmuneresponsesover(1,3),(1,4)-β-D-glucanfrombarley.
Invitrofermentationkineticsandend-productsofcerealarabinoxylansand(1,3;1,4)-β-glucansbyporcinefaeces.
Williams,B.A.,Mikkelsen,D.,LePaih,L.&Gidley,M.J.(2011).JournalofCerealScience,53(1),53-58.
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Purifiedandsemi-purifiedpolysaccharidescharacteristicofcerealswerefermentedinvitrowithapigfaecalinoculum,usingthecumulativegasproductiontechnique,toexaminethekineticsandend-productsoffermentationafter48h.Itwasshownthatarabinoxylanandmixedlinkage(1,3;1,4)β-glucanwererapidlyfermentedifsoluble,whilelesssolublesubstrates(insolublearabinoxylan,maizeandwheatstarchgranules,andbacterialcellulose)weremoreslowlyfermented.Relevantmonosaccharideswerefermentedatverysimilarratestosolublepolymericarabinoxylanandβ-glucan,showingthatdepolymerisationwasnotalimitingstep,incontrasttosomepreviousstudies.Bacterialcelluloseisshowntobeausefulmodelsubstrateforfermentationofplantcellulosewhichisdifficulttoobtainwithoutharshchemicaltreatments.Fermentationend-productswererelatedtokinetics,withslowcarbohydratefermentationresultinginincreasedproteinfermentation.Ratiosofshort-chainfattyacidproductsweresimilarforallarabinoxylanandβ-glucansubstrates.
Roleof(1,3)(1,4)β-glucanincellwalls:Interactionwithcellulose.
Kiemle,S.N.,Zhang,X.,Esker,A.R.,Toriz,G.,Gatenholm,P.&Cosgrove,D.J.(2014).Biomacromolecules,15(5),1727-1736.
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(1,3)(1,4)-β-D-Glucan(mixed-linkageglucanorMLG),acharacteristichemicelluloseinprimarycellwallsofgrasses,wasinvestigatedtodeterminebothitsroleincellwallsanditsinteractionwithcelluloseandothercellwallpolysaccharidesinvitro.BindingisothermsshowedthatMLGadsorptionontomicrocrystallinecelluloseisslow,irreversIBLe,andtemperature-dependent.MeasurementsusingquartzcrystalmicrobalancewithdissipationmonitoringshowedthatMLGadsorbedirreversiblyontoamorphousregeneratedcellulose,formingathickhydrogel.Oligosaccharideprofilingusingendo-(1,3)(1,4)-β-glucanaseindicatedthattherewasnodifferenceinthefrequencyanddistributionof(1,3)and(1,4)linksinboundandunboundMLG.ThebindingofMLGtocellulosewasreducedifthecellulosesampleswerefirsttreatedwithcertaincellwallpolysaccharides,suchasxyloglucanandglucuronoarabinoxylan.ThetetheringfunctionofMLGincellwallswastestedbyapplyingendo-(1,3)(1,4)-β-glucanasetowallsamplesinaconstantforceextensometer.Cellwallextensionwasnotinduced,whichindicatesthatenzyme-accessibleMLGdoesnottethercellulosefibrilsintoaload-bearingnetwork.
Afibrolyticpotentialinthehumanileummucosalmicrobiotarevealedbyfunctionalmetagenomics.
Patrascu,O.,Béguet-Crespel,F.,Marinelli,L.,LeChatelier,E.,Abraham,A.,Leclerc,M.,Klopp,C.,Terrapon,N.,Henrissat,B.,Blottière,H.M.,Doré,J.&ChristelBéra-Maillet.(2017).ScientificReports,7,40248.
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Thedigestionofdietaryfibersisamajorfunctionofthehumanintestinalmicrobiota.Sofarthisfunctionhasbeenattributedtothemicroorganismsinhabitingthecolon,andmanystudieshavefocusedonthisdistalpartofthegastrointestinaltractusingeasilyaccessiblefecalmaterial.However,microbialfermentations,supportedbythepresenceofshort-chainfattyacids,aresUSPectedtooccurintheuppersmallintestine,particularlyintheileum.Usingafosmidlibraryfromthehumanilealmucosa,wescreened20,000clonesfortheiractivitiesagainstcarboxymethylcelluloseandxylanschosenasmodelsofthemajorplantcellwall(PCW)polysaccharidesfromdietaryfibres.ElevenpositiveclonesrevealedabroadrangeofCAZymeencodinggenesfromBacteroidesandClostridialesspecies,aswellasPolysaccharideUtilizationLoci(PULs).Thefunctionalglycosidehydrolasegeneswereidentified,andoligosaccharidebreak-downproductsexaminedfromdifferentpolysaccharidesincludingmixed-linkageβ-glucans.CAZymesandPULswerealsoexaminedfortheirprevalenceinhumangutmicrobiome.Severalclustersofgenesoflowprevalenceinfecalmicrobiomesuggestedtheybelongtounidentifiedstrainsratherspecificallyestablishedupstreamthecolon,intheileum.Thus,theilealmucosa-associatedmicrobiotaencompassestheenzymaticpotentialforPCWpolysaccharidedegradationinthesmallintestine.
Bondsbrokenandformedduringthemixed-linkageglucan:xyloglucanendotransglucosylasereactioncatalysedbyEquisetumhetero-trans-β-glucanase.
Simmons,T.J.&Fry,S.C.(2017).BiochemicalJournal,474(7),1055-1070.
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Mixed-linkageglucan:xyloglucanendotransglucosylase(MXE)isoneofthethreeactivitiesoftherecentlycharacterisedhetero-trans-β-glucanase(HTG),whichamongland-plantsisknownonlyfromEquisetumspecies.ThebiochemicaldetailsoftheMXEreactionwereincompletelyunderstood-detailsthatwouldpromoteunderstandingofMXE"sroleinvivoandenableitsfulltechnologicalexploitation.WeinvestigatedHTG"ssiteofattackononeofitsdonorsubstrates,mixed-linkage(1→3),(1→4)-β-D-glucan(MLG),withradioactiveoligosaccharidesofxyloglucanasacceptorsubstrate.ComparingthreedifferentMLGpreparations,weshowedthattheenzymefavoursthosewithahighcontentofcellotetraoseblocks.Thereactionproductswereanalysedbyenzymicdigestion,thin-layerchromatography,HPLCandgel-permeationchromatography.EquisetumHTGconsistentlycleavedtheMLGatthethirdconsecutiveβ-(1→4)-bondfollowing(towardsthereducingterminus)aβ-(1→3)-bond.Itthenformedaβ-(1→4)-bondbetweentheMLGandthenon-reducingterminalglucoseresidueofthexyloglucanoligosaccharide,consistentwithitsXTHsubfamilymembership.Usingsize-homogeneousbarleyMLGasdonorsubstrate,weshowedthatHTGdoesnotfavouranyparticularregionoftheMLGchainrelativethepolysaccharide"sreducingandnon-reducingtermini;rather,itselectsitstargetcellotetraosylunitstochasticallyalongtheMLGmolecule.Thisworkimprovesourunderstandingofhowenzymescanexhibitpromiscuoussubstratespecificitiesandprovidesthefoundationstoexplorestrategiesforengineeringnovelsubstratespecificitiesintotransglycanases.
Multi-scalecharacterisationofdeuteratedcellulosecompositehydrogelsrevealsevidencefordifferentinteractionmechanismswitharabinoxylan,mixed-linkageglucanandxyloglucan.
Martínez-Sanz,M.,Mikkelsen,D.,Flanagan,B.M.,Gidley,M.J.&Gilbert,E.P.(2017).Polymer,124,1-11.
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Theinteractionsofcellulosewithothermajorplantcellwallpolysaccharides-arabinoxylan(AX),xyloglucan(XG)andmixedlinkageglucans(MLG)-havebeeninvestigatedbycharacterisingthearchitectureofcompositedeuteratedcellulosehydrogelsbymeansofSAXSandSANS,combinedwithXRD,NMRandmicroscopy.Theresultsindicatethatcellulose-AXinteractions,limitedtotheribbons"surface,takeplaceviaanon-specificadsorptionmechanism.Incontrast,XGandMLGinteractspecificallywithcellulose,formingtwodifferentfractions:(i)interfibrillardomainsinteractingwiththecellulosemicrofibrilsand(ii)surfacedomains,responsibleforthecross-linkingofribbons.XGco-crystalliseswithcellulose,promotingtheformationofIβ-richermicrofibrilsandformingintercalatedamorphousregions.Ontheotherhand,MLGinteractswithcelluloseformingaparacrystallinecoatinglayer.ThisstructuralroleofXGandMLGinpreventingmicrofibrilaggregationmayhelpexplaintheirkeyfunctioninthecellexpansionprocessofgrowingplanttissues.
品牌介绍
Megazyme品牌产品简介
来源:作者:人气:2149发表时间:2016-05-19 10:59:00【大 中 小】
Megazyme是一家全球性公司,专注于开发和提供用于饮料、谷物、乳制品、食品、饲料、发酵、生物燃料和葡萄酒产业用的分析试剂、酶和检测试剂盒。Megazyme的许多检测试剂盒产品已经为众多官方科学协会(包括AOAC, AACC , RACI, EBC和ICC等),经过严格的审核,批准认证为官方标准方法,确保以准确、可靠、定量和易于使用的测试方法,满足客户的质量诉求。
Megazyme的主要产品线包括:
◆ 检测试剂盒
◆ 酶
◆ 酶底物
◆ 碳水化合物
◆ 化学品/仪器
官网地址:http://www.megazyme.com
检测试剂盒特色产品:
货号
中文品名
用途
K-ACETAF
乙酸[AF法]检测试剂盒
酶法定量分析乙酸最广泛使用的方法
K-ACHDF
可吸收糖/膳食纤维检测试剂盒
酒精沉淀法测定膳食纤维
K-AMIAR
氨快速检测试剂盒
用于包括葡萄汁、葡萄酒以及其它食品饮料样品中氨含量的快速检测分析。
K-AMYL
直链淀粉/支链淀粉检测试剂盒
谷物淀粉和而粉中直链淀粉/支链淀粉比例和含量检测
K-ARAB
阿拉伯聚糖检测试剂盒
果汁浓缩液中阿拉伯聚糖的检测
K-ASNAM
L-天冬酰胺/L-谷氨酰胺和氨快速检测试剂盒
用于食品工业中丙烯酰胺前体、细胞培养基、以及上清液组分中、L-天冬酰胺,谷氨酰胺和氨的检测分析
K-ASPTM
阿斯巴甜检测试剂盒
专业用于测定饮料和食品中阿斯巴甜含量,操作简单
K-BETA3
β-淀粉酶检测试剂盒
适用于麦芽粉中β-淀粉酶的测定
K-BGLU
混合键β-葡聚糖检测试剂盒
测定谷物、荞麦粉、麦汁、啤酒及其它食品中混合键β-葡聚糖(1,3:1,4-β-D-葡聚糖)的含量
K-CERA
α-淀粉酶检测试剂盒
谷物和发酵液(真菌和细菌)中α-淀粉酶的分析测定
K-CITR
柠檬酸检测试剂盒
快速、可靠地检测食品、饮料和其它物料中柠檬酸(柠檬酸盐)含量
K-DLATE
乳酸快速检测试剂盒
快速、特异性检测饮料、肉类、奶制品和其它食品中L-乳酸和D-乳酸(乳酸盐)含量
K-EBHLG
酵母β-葡聚糖酶检测试剂盒
用于测量和分析酵母中1,3:1,6?-β-葡聚糖,也可以检测1,3-葡聚糖
K-ETSULPH
总亚硫酸检测试剂盒
测定葡萄酒、饮料、食品和其他物料中总亚硫酸含量(按二氧化硫计)的一种简单,高效,可靠的酶法检测方法
K-FRGLMQ
D-果糖/D-葡萄糖[MegaQuant法]检测试剂盒
适用于使用megaquant?色度计(505nm下)测定葡萄、葡萄汁和葡萄酒中D-果糖和D-葡萄糖的含量。
K-FRUC
果聚糖检测试剂盒
含有淀粉、蔗糖和其他糖类的植物提取物和食品中果聚糖的含量测定。
K-FRUGL
D-果糖/D-葡萄糖检测试剂盒
对植物和食品中果糖或葡萄糖含量的酶法紫外分光测定。
K-GALM
半乳甘露聚糖检测试剂盒
食品和植物产品中半乳甘露聚糖的含量检测
K-GLUC
D-葡萄糖[GOPOD]检测试剂盒
谷物提取物中D-葡萄糖的含量测定,可以和其它Megazyme检测试剂盒联合使用。
K-GLUHK
D-葡萄糖[HK]检测试剂盒
植物和食品中D-葡萄糖的含量测定,可以和其它Megazyme检测试剂盒联合使用。
K-GLUM
葡甘聚糖检测试剂盒
植物和食品中葡甘聚糖的含量测定。
K-INTDF
总膳食纤维检测试剂盒
总膳食纤维特定检测和分析
K-LACGAR
乳糖/D-半乳糖快速检测试剂盒
用于快速检测食品和植物产品中乳糖、D-半乳糖和L-阿拉伯糖
K-LACSU
乳糖/蔗糖/D-葡萄糖检测试剂盒
混合面粉和其它物料中蔗糖、乳糖和D-葡萄糖的测定
K-LACTUL
乳果糖检测试剂盒
特异性、快速和灵敏测量奶基样品中乳果糖含量
K-MANGL
D-甘露糖/D-果糖/D-葡萄糖检测试剂盒
适合测定植物产品和多糖酸性水解产物中D-甘露糖含量
K-MASUG
麦芽糖/蔗糖/D-葡萄糖检测试剂盒
在植物和食品中麦芽糖,蔗糖和葡萄糖的含量检测
K-PECID
胶质识别检测试剂盒
食品配料中果胶的鉴别
K-PHYT
植酸(总磷)检测试剂盒
食品和饲料样品植酸/总磷含量测量的简便方法。不需要通过阴离子交换色谱对植酸纯化,适合于大量样本分析
K-PYRUV
丙酮酸检测试剂盒
在啤酒、葡萄酒、果汁、食品和体液中丙酮酸分析
K-RAFGA
棉子糖/D-半乳糖检测试剂盒
快速测量植物材料和食品中棉子糖和半乳糖含量
K-RAFGL
棉子糖/蔗糖/D-半乳糖检测试剂盒
分析种子和种子粉中D-葡萄糖、蔗糖、棉子糖、水苏糖和毛蕊花糖含量。通过将棉子糖、水苏糖和毛蕊花糖酶解D-葡萄糖、D-果糖和半乳糖,从而测定葡萄糖含量来确定
K-SDAM
淀粉损伤检测试剂盒
谷物面粉中淀粉损伤的检测和分析
K-SUCGL
蔗糖/D-葡萄糖检测试剂盒
饮料、果汁、蜂蜜和食品中蔗糖和葡萄糖的分析
K-SUFRG
蔗糖/D-果糖/D-葡萄糖检测试剂盒
适用于植物和食品中蔗糖、D-葡萄糖和D-果糖的测定
K-TDFR
总膳食纤维检测试剂盒
总膳食纤维检测
K-TREH
海藻糖检测试剂盒
快速、可靠地检测食品、饮料和其它物料中海藻糖含量
K-URAMR
尿素/氨快速检测试剂盒
适用于水、饮料、乳制品和食品中尿素和氨的快速测定
K-URONIC
D-葡萄糖醛酸/D-半乳糖醛酸检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中六元糖醛酸含量(D-葡萄糖醛酸和D-半乳糖醛酸)
K-XYLOSE
D-木糖检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中D-木糖含量
K-YBGL
Beta葡聚糖[酵母和蘑菇]检测试剂盒
检测酵母和蘑菇制品中1,3:1,6-beta-葡聚糖和α-葡聚糖含量
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