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Megazyme/果胶半乳聚糖(羽扇豆)/P-PGALU/4克
商品编号:
P-PGALU
品牌:
Megazyme INC
市场价:
¥3288.00
美元价:
1972.80
产品分类:
其他试剂
公司分类:
Other_reagents
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
HighpurityPecticGalactan(Lupin)foruseinresearch,biochemicalenzymeassaysandinvitrodiagnosticanalysis.
Highlypurified,water-solublepolysaccharideextractedwithalkalifromlupinfiber.Gal:Ara:Rha:Xyl:GalUA=77:14:3:0.6:5.4
Effectofnanocoatingwithrhamnogalacturonan‐Ionsurfacepropertiesandosteoblastsresponse.
Gurzawska,K.,Svava,R.,Syberg,S.,Yihua,Y.,Haugshøj,K.B.,Damager,I.,Ulvskov,P.,Christensen,L.H.,Gotfredsen,K.&Jørgensen,N.R.(2012).JournalofBiomedicalMaterialsResearchPartA,100(3),654-664.
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Long-termstABIlityoftitaniumimplantsaredependentonavarietyoffactors.Nanocoatingwithorganicmoleculesisoneofthemethodsusedtoimproveosseointegration.Therefore,theaimofthisstudyistoevaluatetheinvitroeffectofnanocoatingwithpecticrhamnogalacturonan-I(RG-I)onsurfacepropertiesandosteoblastsresponse.ThreedifferentRG-Isfromappleandlupinpectinsweremodifiedandcoatedonamino-functionalizedtissueculturepolystyreneplates(aminatedTCPS).Surfacepropertieswereevaluatedbyscanningelectronmicroscopy,contactanglemeasurement,atomicforcemicroscopy,andX-rayphotoelectronspectroscopy.Theeffectsofnanocoatingonproliferation,matrixformationandmineralization,andexpressionofgenes(real-timePCR)relatedtoosteoblastdifferentiationandactivityweretestedusinghumanosteoblast-likeSaOS-2cells.ItwasshownthatRG-Icoatingsaffectedthesurfaceproperties.AllthreeRG-Iinducedbonematrixformationandmineralization,whichwasalsosupportedbythefindingthatgeneexpressionlevelsofalkalinephosphatase,osteocalcin,andcollagentype-1wereincreasedincellsculturedontheRG-Icoatedsurface,indicatingamoredifferentiatedosteoblasticphenotype.ThismakesRG-Icoatingapromisingandnovelcandidatefornanocoatingsofimplants.
Characterizationofanexo-β-1,3-galactanasefromClostridiumThermocellum.
Ichinose,H.,Kuno,A.,Kotake,T.,Yoshida,M.,Sakka,K.,Hirabayashi,J.,Tsumuraya,Y.&Kaneko,S.(2006).AppliedandEnvironmentalMicroBIOLOGy,72(5),3515-3523.
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Ageneencodinganexo-β-1,3-galactanasefromClostridiumthermocellum,Ct1,3Gal43A,wasisolated.Thesequencehassimilaritywithanexo-β-1,3-galactanaseofPhanerochaetechrysosporium(Pc1,3Gal43A).ThegeneencodesamodularproteinconsistingofanN-terminalglycosidehydrolasefamily43(GH43)module,afamily13carbohydrate-bindingmodule(CBM13),andaC-terminaldockerindomain.ThegenecorrespondingtotheGH43modulewasexpressedinEscherichiacoli,andthegeneproductwascharacterized.TherecombinantenzymeshowsoptimalactivityatpH6.0and50°Candcatalyzeshydrolysisonlyofβ-1,3-linkedgalactosyloligosaccharidesandpolysaccharides.High-performanceliquidchromatographyanalysisofthehydrolysisproductsdemonstratedthattheenzymeproducesgalactosefromβ-1,3-galactaninanexo-actingmanner.Whentheenzymeactedonarabinogalactanproteins(AGPs),theenzymeproducedoligosaccharidestogetherwithgalactose,suggestingthattheenzymeisabletoaccommodateaβ-1,6-linkedgalactosylsidechain.ThesubstratespecificityoftheenzymeisverysimilartothatofPc1,3Gal43A,suggestingthattheenzymeisanexo-β-1,3-galactanase.AffinitygelelectrophoresisoftheC-terminalCBM13didnotshowanyaffinityforpolysaccharides,includingβ-1,3-galactan.However,frontalaffinitychromatographyfortheCBM13indicatedthattheCBM13specificallyinteractswitholigosaccharidescontainingaβ-1,3-galactobiose,β-1,4-galactosylglucose,orβ-1,4-galactosylN-acetylglucosaminidemoietyatthenonreducingend.Interestingly,CBM13intheCterminusofCt1,3Gal43Aappearedtointerferewiththeenzymeactivitytowardβ-1,3-galactanandα-L-arabinofuranosidase-treatedAGP.
Anexo-β-1,3-galactanasehavinganovelβ-1,3-galactan-bindingmodulefromPhanerochaetechrysosporium.
Ichinose,H.,Yoshida,M.,Kotake,T.,Kuno,A.,Igarashi,K.,Tsumuraya,Y.,Samejima,M.,Hirabayashi,J.,Kobayashi,H.&Kaneko,S.(2005).JournalofBiologicalChemistry,280(27),25820-25829.
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Anexo-β-1,3-galactanasegenefromPhanerochaetechrysosporiumhasbeencloned,sequenced,andexpressedinPichiapastoris.Thecompleteaminoacidsequenceoftheexo-β-1,3-galactanaseindicatedthattheenzymeconsistsofanN-terminalcatalyticmodulewithsimilaritytoglycosidehydrolasefamily43andanadditionalunknownfunctionaldomainsimilartocarbohydrate-bindingmodulefamily6(CBM6)intheC-terminalregion.Themolecularmassoftherecombinantenzymewasestimatedas55kDabasedonSDS-PAGE.Theenzymeshowedreactivityonlytowardβ-1,3-linkedgalactosyloligosaccharidesandpolysaccharideassubstratesbutdidnothydrolyzeβ-1,4-linkedgalacto-oligosaccharides,β-1,6-linkedgalacto-oligosaccharides,pecticgalactan,larcharabinogalactan,arabinan,gumarabic,debranchedarabinan,laminarin,solublebirchwoodxylan,orsolubleoatspelledxylan.Theenzymealsodidnothydrolyzeβ-1,3-galactosylgalactosaminide,β-1,3-galactosylglucosaminide,orβ-1,3-galactosylarabinofuranoside,suggestingthatitspecificallycleavestheinternalβ-1,3-linkageoftwogalactosylresidues.Highperformanceliquidchromatographicanalysisofthehydrolysisproductsshowedthattheenzymeproducedgalactosefromβ-1,3-galactaninanexo-actingmanner.However,noactivitytowardp-nitrophenylβ-galactopyranosidewasdetected.Whenincubatedwitharabinogalactanproteins,theenzymeproducedoligosaccharidestogetherwithgalactose,suggestingthatitisabletobypassβ-1,6-linkedgalactosylsidechains.TheC-terminalCBM6didnotshowanyaffinityforknownsubstratesofCBM6suchasxylan,cellulose,andβ-1,3-glucan,althoughitboundβ-1,3-galactanwhenanalyzedbyaffinityelectrophoresis.FrontalaffinitychromatographyfortheCBM6moietyusingseveralkindsofterminalgalactose-containingoligosaccharidesastheanalytesclearlyindicatedthattheCBM6specificallyinteractedwitholigosaccharidescontainingaβ-1,3-galactobiosemoiety.Whenthedegreeofpolymerizationofgalactoseoligomerswasincreased,thebindingaffinityoftheCBM6showednomarkedchange.
Family6carbohydrate‐bindingmodulesdisplaymultipleβ1,3‐linkedglucan‐specificbindinginterfaces.
Correia,M.A.S.,Pires,V.M.R.,Gilbert,H.J.,Bolam,D.N.,Fernandes,V.O.,Alves,V.D.,Prates,J.A.M.,Ferreira,L.M.A.&Fontes,C.M.G.(2009).FEMSMicrobiologyLetters,300(1),48-57.
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Noncatalyticcarbohydrate-bindingmodules(CBMs),whicharefoundinavarietyofcarbohydrate-degrADIngenzymes,havebeengroupedintosequence-basedfamilies.CBMs,byrecruitingtheirappendedenzymesontothesurfaceofthetargetsubstrate,potentiatecatalysisparticularlyagainstinsolublesubstrates.Family6CBMs(CBM6s)displayunusualpropertiesinthattheypresenttwopotentialligand-bindingsitestermedcleftsAandB,respectively.CleftBislocatedontheconcavesurfaceoftheβ-sandwichfoldwhilecleftA,themorecommonbindingsite,isformedbytheloopsthatconnecttheinnerandtheouterβ-sheets.Here,wereportthebiochemicalpropertiesofCBM6-1fromCellvibriomixtusCmCel5A.ThedatarevealthatCBM6-1specificallyrecognizesβ1,3-glucansthroughresidueslocatedbothincleftAandincleftB.Incontrast,apreviousreportshowedthataCBM6derivedfromaBacillushaloduranslaminarinasebindstoβ1,3-glucansonlyincleftA.Thesestudiesrevealadifferentmechanismbywhichahighlyconservedproteinplatformcanrecognizeβ1,3-glucans.
Real-timeimagingofcellulosereorientationduringcellwallexpansioninArabidopsisroots.
Anderson,C.T.,Carroll,A.,Akhmetova,L.&Somerville,C.(2010).PlantPhysiology,152(2),787-796.
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Celluloseformsthemajorload-bearingnetworkoftheplantcellwall,whichsimultaneouslyprotectsthecellanddirectsitsgrowth.Althoughtheprocessofcellulosesynthesishasbeenobserved,littleisknownaboutthebehaviorofcelluloseinthewallaftersynthesis.UsingPontamineFastScarlet4B,adyethatfluorescespreferentiallyinthepresenceofcelluloseandhasexcitationandemissionwavelengthssuitableforconfocalmicroscopy,weimagedthearchitectureanddynamicsofcelluloseinthecellwallsofexpandingrootcells.WefoundthatcelluloseexistsinArabidopsis(Arabidopsisthaliana)cellwallsinlargefibrillarbundlesthatvaryinorientation.Duringanisotropicwallexpansioninwild-typeplants,weobservedthatthesecellulosebundlesrotateinatransversetolongitudinaldirection.Wealsofoundthatcelluloseorganizationissignificantlyalteredinmutantslackingeitheracellulosesynthasesubunitortwoxyloglucanxylosyltransferaseisoforms.Ourresultssupportamodelinwhichcelluloseisdepositedtransverselytoaccommodatelongitudinalcellexpansionandreorientedduringexpansiontogenerateacellwallthatisfortifiedagainststrainfromanydirection.
品牌介绍
Megazyme品牌产品简介
来源:作者:人气:2149发表时间:2016-05-19 10:59:00【大 中 小】
Megazyme是一家全球性公司,专注于开发和提供用于饮料、谷物、乳制品、食品、饲料、发酵、生物燃料和葡萄酒产业用的分析试剂、酶和检测试剂盒。Megazyme的许多检测试剂盒产品已经为众多官方科学协会(包括AOAC, AACC , RACI, EBC和ICC等),经过严格的审核,批准认证为官方标准方法,确保以准确、可靠、定量和易于使用的测试方法,满足客户的质量诉求。
Megazyme的主要产品线包括:
◆ 检测试剂盒
◆ 酶
◆ 酶底物
◆ 碳水化合物
◆ 化学品/仪器
官网地址:http://www.megazyme.com
检测试剂盒特色产品:
货号
中文品名
用途
K-ACETAF
乙酸[AF法]检测试剂盒
酶法定量分析乙酸最广泛使用的方法
K-ACHDF
可吸收糖/膳食纤维检测试剂盒
酒精沉淀法测定膳食纤维
K-AMIAR
氨快速检测试剂盒
用于包括葡萄汁、葡萄酒以及其它食品饮料样品中氨含量的快速检测分析。
K-AMYL
直链淀粉/支链淀粉检测试剂盒
谷物淀粉和而粉中直链淀粉/支链淀粉比例和含量检测
K-ARAB
阿拉伯聚糖检测试剂盒
果汁浓缩液中阿拉伯聚糖的检测
K-ASNAM
L-天冬酰胺/L-谷氨酰胺和氨快速检测试剂盒
用于食品工业中丙烯酰胺前体、细胞培养基、以及上清液组分中、L-天冬酰胺,谷氨酰胺和氨的检测分析
K-ASPTM
阿斯巴甜检测试剂盒
专业用于测定饮料和食品中阿斯巴甜含量,操作简单
K-BETA3
β-淀粉酶检测试剂盒
适用于麦芽粉中β-淀粉酶的测定
K-BGLU
混合键β-葡聚糖检测试剂盒
测定谷物、荞麦粉、麦汁、啤酒及其它食品中混合键β-葡聚糖(1,3:1,4-β-D-葡聚糖)的含量
K-CERA
α-淀粉酶检测试剂盒
谷物和发酵液(真菌和细菌)中α-淀粉酶的分析测定
K-CITR
柠檬酸检测试剂盒
快速、可靠地检测食品、饮料和其它物料中柠檬酸(柠檬酸盐)含量
K-DLATE
乳酸快速检测试剂盒
快速、特异性检测饮料、肉类、奶制品和其它食品中L-乳酸和D-乳酸(乳酸盐)含量
K-EBHLG
酵母β-葡聚糖酶检测试剂盒
用于测量和分析酵母中1,3:1,6?-β-葡聚糖,也可以检测1,3-葡聚糖
K-ETSULPH
总亚硫酸检测试剂盒
测定葡萄酒、饮料、食品和其他物料中总亚硫酸含量(按二氧化硫计)的一种简单,高效,可靠的酶法检测方法
K-FRGLMQ
D-果糖/D-葡萄糖[MegaQuant法]检测试剂盒
适用于使用megaquant?色度计(505nm下)测定葡萄、葡萄汁和葡萄酒中D-果糖和D-葡萄糖的含量。
K-FRUC
果聚糖检测试剂盒
含有淀粉、蔗糖和其他糖类的植物提取物和食品中果聚糖的含量测定。
K-FRUGL
D-果糖/D-葡萄糖检测试剂盒
对植物和食品中果糖或葡萄糖含量的酶法紫外分光测定。
K-GALM
半乳甘露聚糖检测试剂盒
食品和植物产品中半乳甘露聚糖的含量检测
K-GLUC
D-葡萄糖[GOPOD]检测试剂盒
谷物提取物中D-葡萄糖的含量测定,可以和其它Megazyme检测试剂盒联合使用。
K-GLUHK
D-葡萄糖[HK]检测试剂盒
植物和食品中D-葡萄糖的含量测定,可以和其它Megazyme检测试剂盒联合使用。
K-GLUM
葡甘聚糖检测试剂盒
植物和食品中葡甘聚糖的含量测定。
K-INTDF
总膳食纤维检测试剂盒
总膳食纤维特定检测和分析
K-LACGAR
乳糖/D-半乳糖快速检测试剂盒
用于快速检测食品和植物产品中乳糖、D-半乳糖和L-阿拉伯糖
K-LACSU
乳糖/蔗糖/D-葡萄糖检测试剂盒
混合面粉和其它物料中蔗糖、乳糖和D-葡萄糖的测定
K-LACTUL
乳果糖检测试剂盒
特异性、快速和灵敏测量奶基样品中乳果糖含量
K-MANGL
D-甘露糖/D-果糖/D-葡萄糖检测试剂盒
适合测定植物产品和多糖酸性水解产物中D-甘露糖含量
K-MASUG
麦芽糖/蔗糖/D-葡萄糖检测试剂盒
在植物和食品中麦芽糖,蔗糖和葡萄糖的含量检测
K-PECID
胶质识别检测试剂盒
食品配料中果胶的鉴别
K-PHYT
植酸(总磷)检测试剂盒
食品和饲料样品植酸/总磷含量测量的简便方法。不需要通过阴离子交换色谱对植酸纯化,适合于大量样本分析
K-PYRUV
丙酮酸检测试剂盒
在啤酒、葡萄酒、果汁、食品和体液中丙酮酸分析
K-RAFGA
棉子糖/D-半乳糖检测试剂盒
快速测量植物材料和食品中棉子糖和半乳糖含量
K-RAFGL
棉子糖/蔗糖/D-半乳糖检测试剂盒
分析种子和种子粉中D-葡萄糖、蔗糖、棉子糖、水苏糖和毛蕊花糖含量。通过将棉子糖、水苏糖和毛蕊花糖酶解D-葡萄糖、D-果糖和半乳糖,从而测定葡萄糖含量来确定
K-SDAM
淀粉损伤检测试剂盒
谷物面粉中淀粉损伤的检测和分析
K-SUCGL
蔗糖/D-葡萄糖检测试剂盒
饮料、果汁、蜂蜜和食品中蔗糖和葡萄糖的分析
K-SUFRG
蔗糖/D-果糖/D-葡萄糖检测试剂盒
适用于植物和食品中蔗糖、D-葡萄糖和D-果糖的测定
K-TDFR
总膳食纤维检测试剂盒
总膳食纤维检测
K-TREH
海藻糖检测试剂盒
快速、可靠地检测食品、饮料和其它物料中海藻糖含量
K-URAMR
尿素/氨快速检测试剂盒
适用于水、饮料、乳制品和食品中尿素和氨的快速测定
K-URONIC
D-葡萄糖醛酸/D-半乳糖醛酸检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中六元糖醛酸含量(D-葡萄糖醛酸和D-半乳糖醛酸)
K-XYLOSE
D-木糖检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中D-木糖含量
K-YBGL
Beta葡聚糖[酵母和蘑菇]检测试剂盒
检测酵母和蘑菇制品中1,3:1,6-beta-葡聚糖和α-葡聚糖含量
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