Megazyme/聚半乳糖醛酸（PGA）/P-PGACT/10克
商品编号：
P-PGACT
品牌：
Megazyme INC
市场价：
¥2688.00
美元价：
1612.80
产品分类：
其他试剂
公司分类：
Other_reagents
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
HighpurityPolygalacturonicAcid(PGA)foruseinresearch,biochemicalenzymeassaysandinvitrodiagnosticanalysis.
Purity>95%.
Arevisedarchitectureofprimarycellwallsbasedonbiomechanicalchangesinducedbysubstrate-specificendoglucanases.
Park,Y.B.&Cosgrove,D.J.(2012).PlantPhysiology,158(4),1933-1943.
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Xyloglucaniswidelybelievedtofunctionasatetherbetweencellulosemicrofibrilsintheprimarycellwall,limitingcellenlargementbyrestrictingtheABIlityofmicrofibrilstoseparatelaterally.Totestthebiomechanicalpredictionsofthis“tetherednetwork”model,weassessedtheabilityofcucumber(Cucumissativus)hypocotylwallstoundergocreep(long-term,irreversIBLeextension)inresponsetothreefamily-12endo-β-1,4-glucanasesthatcanspecificallyhydrolyzexyloglucan,cellulose,orboth.Xyloglucan-specificendoglucanase(XEGfromAspergillusaculeatus)failedtoinducecellwallcreep,whereasanendoglucanasethathydrolyzesbothxyloglucanandcellulose(Cel12AfromHypocreajecorina)inducedahighcreeprate.Acellulose-specificendoglucanase(CEGfromAspergillusniger)didnotcausecellwallcreep,eitherbyitselforincombinationwithXEG.Testswithadditionalenzymes,includingafamily-5endoglucanase,confirmedtheconclusionthattocausecreep,endoglucanasesmustcutbothxyloglucanandcellulose.Similarresultswereobtainedwithmeasurementsofelasticandplasticcompliance.BothXEGandCel12Ahydrolyzedxyloglucaninintactwalls,butCel12AcouldhydrolyzeaminorxyloglucancompartmentrecalcitranttoXEGdigestion.XyloglucaninvolvementintheseenzymeresponseswasconfirmedbyexperimentswithArabidopsis(Arabidopsisthaliana)hypocotyls,whereCel12Ainducedcreepinwild-typebutnotinxyloglucan-deficient(xxt1/xxt2)walls.Ourresultsareincompatiblewiththecommondepictionofxyloglucanasaload-bearingtetherspanningthe20-to40-nmspacingbetweencellulosemicrofibrils,buttheydoimplicateaminorxyloglucancomponentinwallmechanics.Thestructurallyimportantxyloglucanmaybelocatedinlimitedregionsoftightcontactbetweenmicrofibrils.
ProductionoptimizationandexpressionofpectinreleasingenzymefromAspergillusoryzaePO.
Chen,J.,Yang,R.,Chen,M.,Wang,S.,Li,P.,Xia,Y.,Zhou,L.,Xie,J.&Wei,D.(2014).CarbohydratePolymers,101,89-95.
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Protopectinaseisanenzymethatsolubilizesprotopectinforminghighlypolymerizedsolublepectin.ProtopectinaseactivitywasdetectedfromAspergillusoryzaePOisolatedfromsoilofpersimmonorchard.ResponsesurfacemethodologyofBox–BehnkenDesignwiththreefermentationvariables(temperature,NaNO3andapplepomaceconcentration)wasusedtooptimizeprotopectinaseproductionofA.oryzaePO,andprotopectinaseactivitywasimprovedto270.0U/ml.Endo-polygalacturonasebelongedtoA-typePPasefromA.oryzaePOwasclonedandexpressedinPichiapastorisGS115.Theendo-polygalacturonaseexpressionwas0.418mg/mlandthespecificactivityofpurifiedrecombinantendo-polygalacturonasewas7520U/mgtowardpolygalacturonicacid.TheoptimaltemperatureandpHofrecombinantendo-polygalacturonasewere45°Cand5.0,respectively.Therecombinantendo-polygalacturonaseactivitywasenhancedbythepresenceofMg2+,whileCa2+,Ni2+Mn2+,Cu2+andSDSstronglyinhibitedtheenzymeactivity.TheapparentKmvalueandVmaxvaluewere5.59mg/mland1.01µmol/(minml),respectively.
UsingAFMandforcespectroscopytodeterminepectinstructureand(bio)functionality.
Morris,V.J.,Gromer,A.,Kirby,A.R.J.,Bongaerts,R.J.M.&PatrickGunning,A.(2011).FoodHydrocolloids,25(2),230-237.
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Pectinisanintegralcomponentofnon-graminaceousplantcellwalls.Itisbelievedtoformaninterconnectednetworkstructureindependentofthecellulose-xyloglucannetworkstructure.Pectingelsareoftenusedasamodelforthepectinnetworkstructurewithintheplantcellwall.Atomicforcemicroscopystudiesofcalcium-inducedgelprecursors,andfragmentsreleasedfromgels,suggestthatassociationleadstoabranchedfibrousstructurewithinthegels.Enzymaticde-esterificationofhigh-methoxylpectininthepresenceofcalciumionscaninducegelationofthepectin.Thuspectingelnetworksmayprovideamodelforaself-assemblednetworkstructurewithinthemiddlelamellaregionoftheplantcellwall.Thepectinnetworkinplantcellwallsisasourceofsolubleandinsolublefibre.Inadditiontothehealthbenefitsassociatedwiththedietaryfibreaspectsofpectinnewhealthclaimsareemerging.Recentlypublishedinvitroandinvivoanimalstudies,andhumanstudies,suggestthatoralconsumptionofamodifiedformofpectinmayhaveanti-cancerproperties.Thesestudiessuggestthatthemodifiedpectinmayactonarangeofcancersatseveralstagesofprogressionofthecancer.Ithasbeenhypothesisedthatthisgenericactionisduetothemodificationallowingreleaseofbioactivefragment(s)whichareclaimedtobindspecificallytoandinhibittheactionofthemammalianlectingalectin3(Gal3).Gal3isakeyregulatorofcellularhomeostasisandplaysimportantrolesinseveralstagesofcancermetastasis.Studiesusingforcespectroscopy,flowcytometryandfluorescencemicroscopysuggestthatthebioactivefragmentsofpectinmaybepectin-derivedgalactans.
Delayedutilizationofsomefast-fermentingsolubledietaryfibersbyhumangutmicrobiotawhenpresentedinamixture.
Tuncil,Y.E.,Nakatsu,C.H.,Kazem,A.E.,Arioglu-Tuncil,S.,Reuhs,B.,Martens,E.C.&Hamaker,B.R.(2017).JournalofFunctionalFoods,32,347-357.
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Deliveringfibersmoredistallycouldbeimportanttopreventortreatcolonicdiseasessuchascancerandulcerativecolitis.Here,wehypothesizedthatfermentationoffast-fermentingsolublefibersbythecolonicmicrobiotaisdelayedwhentheyarepresentedinamixtureduetohierarchicalutilizationoffibers.Aseriesofinvitrofermentationstudieswasperformedusingfecalmicrobiotaobtainedfromthreehealthydonorsusingsingledietaryfibers[arabinoxylan,chondroitinsulfate(CS),galactomannan(GM),polygalacturonicacid(PGA),xyloglucan(XG)]andamixturecontaininganequalamountofeach.Substratedisappearanceanalysis,asmeasuredbyGC–MS,revealedthatCS,PGA,andXGutilizationwasdelayedwhenpresentinthemixture.16SrRNAsequencingshowedcertainfibersconsistentlyincreasedspecificgenerainthemicrobiotaofalldonorgroups.Mixingdifferenttypesoffermentabledietaryfibersmightbealogicalstrategyfordeliveringfibersintomoredistalregionsofthecolon.
ReciprocalPrioritizationtoDietaryGlycansbyGutBacteriainaCompetitiveEnvironmentPromotesStableCoexistence.
Tuncil,Y.E.,Xiao,Y.,Porter,N.T.,Reuhs,B.L.,Martens,E.C.&Hamaker,B.R.(2017).mBio,8(5),e01068-17.
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Whenpresentedwithnutrientmixtures,severalhumangutBacteroidesspeciesexhibithierarchicalutilizationofglycansthroughaphenomenonthatresemblescataboliterepression.However,itisunclearhowcloselytheseobservedphysiologicalchanges,oftenmeasuredbyalteredtranscriptionofglycanutilizationgenes,mirroractualglycandepletion.Tounderstandtheglycanprioritizationstrategiesoftwocloselyrelatedhumangutsymbionts,BacteroidesovatusandBacteroidesthetaiotaomicron,weperformedaseriesoftimecourseassaysinwhichbothspecieswereindividuallygrowninamediumwithsixdifferentglycansthatbothspeciescandegrade.Disappearanceofthesubstratesandtranscriptionofthecorrespondingpolysaccharideutilizationloci(PULs)weremeasured.Eachspeciesutilizedsomeglycansbeforeothers,butwithdifferentprioritiesperspecies,providinginsightintospecies-specifichierarchicalpreferences.Ingeneral,thepresenceofhighlyprioritizedglycansrepressedtranscriptionofgenesinvolvedinutilizinglower-prioritynutrients.However,transcriptionalsensitivitytosomeglycansvariedrelativetotheresidualconcentrationinthemedium,withsomePULsthattargethigh-prioritysubstratesremaininghighlyexpressedevenaftertheirtargetglycanhadbeenmostlydepleted.Coculturingoftheseorganismsinthesamemixtureshowedthatthehierarchicalordersgenerallyremainedthesame,promotingstablecoexistence.Polymerlengthwasfoundtobeacontributingfactorforglycanutilization,therebyaffectingitsplaceinthehierarchy.OurfindingsnotonlyelucidatehowB.ovatusandB.thetaiotaomicronstrategicallyaccessglycanstomaintaincoexistencebutalsosupporttheprioritizationofcarbohydrateutilizationbasedoncarbohydratestructure,advancingourunderstandingoftherelationshipsbetweendietandthegutmicrobiome.
品牌介绍
Megazyme品牌产品简介
来源：作者：人气：2149发表时间：2016-05-19 10:59:00【大 中 小】
Megazyme是一家全球性公司，专注于开发和提供用于饮料、谷物、乳制品、食品、饲料、发酵、生物燃料和葡萄酒产业用的分析试剂、酶和检测试剂盒。Megazyme的许多检测试剂盒产品已经为众多官方科学协会（包括AOAC, AACC , RACI, EBC和ICC等），经过严格的审核，批准认证为官方标准方法，确保以准确、可靠、定量和易于使用的测试方法，满足客户的质量诉求。
Megazyme的主要产品线包括：
◆ 检测试剂盒
◆ 酶
◆ 酶底物
◆ 碳水化合物
◆ 化学品/仪器
官网地址：http://www.megazyme.com
检测试剂盒特色产品：
货号
中文品名
用途
K-ACETAF
乙酸[AF法]检测试剂盒
酶法定量分析乙酸最广泛使用的方法
K-ACHDF
可吸收糖/膳食纤维检测试剂盒
酒精沉淀法测定膳食纤维
K-AMIAR
氨快速检测试剂盒
用于包括葡萄汁、葡萄酒以及其它食品饮料样品中氨含量的快速检测分析。
K-AMYL
直链淀粉/支链淀粉检测试剂盒
谷物淀粉和而粉中直链淀粉/支链淀粉比例和含量检测
K-ARAB
阿拉伯聚糖检测试剂盒
果汁浓缩液中阿拉伯聚糖的检测
K-ASNAM
L-天冬酰胺/L-谷氨酰胺和氨快速检测试剂盒
用于食品工业中丙烯酰胺前体、细胞培养基、以及上清液组分中、L-天冬酰胺，谷氨酰胺和氨的检测分析
K-ASPTM
阿斯巴甜检测试剂盒
专业用于测定饮料和食品中阿斯巴甜含量，操作简单
K-BETA3
β-淀粉酶检测试剂盒
适用于麦芽粉中β-淀粉酶的测定
K-BGLU
混合键β-葡聚糖检测试剂盒
测定谷物、荞麦粉、麦汁、啤酒及其它食品中混合键β-葡聚糖(1,3:1,4-β-D-葡聚糖)的含量
K-CERA
α-淀粉酶检测试剂盒
谷物和发酵液（真菌和细菌）中α-淀粉酶的分析测定
K-CITR
柠檬酸检测试剂盒
快速、可靠地检测食品、饮料和其它物料中柠檬酸（柠檬酸盐）含量
K-DLATE
乳酸快速检测试剂盒
快速、特异性检测饮料、肉类、奶制品和其它食品中L-乳酸和D-乳酸(乳酸盐）含量
K-EBHLG
酵母β-葡聚糖酶检测试剂盒
用于测量和分析酵母中1,3:1,6?-β-葡聚糖，也可以检测1,3-葡聚糖
K-ETSULPH
总亚硫酸检测试剂盒
测定葡萄酒、饮料、食品和其他物料中总亚硫酸含量（按二氧化硫计）的一种简单，高效，可靠的酶法检测方法
K-FRGLMQ
D-果糖/D-葡萄糖[MegaQuant法]检测试剂盒
适用于使用megaquant?色度计（505nm下）测定葡萄、葡萄汁和葡萄酒中D-果糖和D-葡萄糖的含量。
K-FRUC
果聚糖检测试剂盒
含有淀粉、蔗糖和其他糖类的植物提取物和食品中果聚糖的含量测定。
K-FRUGL
D-果糖/D-葡萄糖检测试剂盒
对植物和食品中果糖或葡萄糖含量的酶法紫外分光测定。
K-GALM
半乳甘露聚糖检测试剂盒
食品和植物产品中半乳甘露聚糖的含量检测
K-GLUC
D-葡萄糖[GOPOD]检测试剂盒
谷物提取物中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUHK
D-葡萄糖[HK]检测试剂盒
植物和食品中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUM
葡甘聚糖检测试剂盒
植物和食品中葡甘聚糖的含量测定。
K-INTDF
总膳食纤维检测试剂盒
总膳食纤维特定检测和分析
K-LACGAR
乳糖/D-半乳糖快速检测试剂盒
用于快速检测食品和植物产品中乳糖、D-半乳糖和L-阿拉伯糖
K-LACSU
乳糖/蔗糖/D-葡萄糖检测试剂盒
混合面粉和其它物料中蔗糖、乳糖和D-葡萄糖的测定
K-LACTUL
乳果糖检测试剂盒
特异性、快速和灵敏测量奶基样品中乳果糖含量
K-MANGL
D-甘露糖/D-果糖/D-葡萄糖检测试剂盒
适合测定植物产品和多糖酸性水解产物中D-甘露糖含量
K-MASUG
麦芽糖/蔗糖/D-葡萄糖检测试剂盒
在植物和食品中麦芽糖，蔗糖和葡萄糖的含量检测
K-PECID
胶质识别检测试剂盒
食品配料中果胶的鉴别
K-PHYT
植酸(总磷)检测试剂盒
食品和饲料样品植酸/总磷含量测量的简便方法。不需要通过阴离子交换色谱对植酸纯化，适合于大量样本分析
K-PYRUV
丙酮酸检测试剂盒
在啤酒、葡萄酒、果汁、食品和体液中丙酮酸分析
K-RAFGA
棉子糖/D-半乳糖检测试剂盒
快速测量植物材料和食品中棉子糖和半乳糖含量
K-RAFGL
棉子糖/蔗糖/D-半乳糖检测试剂盒
分析种子和种子粉中D-葡萄糖、蔗糖、棉子糖、水苏糖和毛蕊花糖含量。通过将棉子糖、水苏糖和毛蕊花糖酶解D-葡萄糖、D-果糖和半乳糖，从而测定葡萄糖含量来确定
K-SDAM
淀粉损伤检测试剂盒
谷物面粉中淀粉损伤的检测和分析
K-SUCGL
蔗糖/D-葡萄糖检测试剂盒
饮料、果汁、蜂蜜和食品中蔗糖和葡萄糖的分析
K-SUFRG
蔗糖/D-果糖/D-葡萄糖检测试剂盒
适用于植物和食品中蔗糖、D-葡萄糖和D-果糖的测定
K-TDFR
总膳食纤维检测试剂盒
总膳食纤维检测
K-TREH
海藻糖检测试剂盒
快速、可靠地检测食品、饮料和其它物料中海藻糖含量
K-URAMR
尿素/氨快速检测试剂盒
适用于水、饮料、乳制品和食品中尿素和氨的快速测定
K-URONIC
D-葡萄糖醛酸/D-半乳糖醛酸检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中六元糖醛酸含量（D-葡萄糖醛酸和D-半乳糖醛酸）
K-XYLOSE
D-木糖检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中D-木糖含量
K-YBGL
Beta葡聚糖[酵母和蘑菇]检测试剂盒
检测酵母和蘑菇制品中1,3:1,6-beta-葡聚糖和α-葡聚糖含量
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