Megazyme/Rhamnogalacturonan I（马铃薯）/P-RHAM1/2克
商品编号：
P-RHAM1
品牌：
Megazyme INC
市场价：
¥2976.00
美元价：
1785.60
产品分类：
其他试剂
公司分类：
Other_reagents
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
HighpurityRhamnogalacturonanI(Potato)foruseinresearch,biochemicalenzymeassaysandinvitrodiagnosticanalysis.
Preparedfrompecticgalactanfrompotatofiber.Anexcellentsubstratefortheassayofrhamnogalacturonanhydrolaseandrhamnogalacturonanlyase.
CompletegenomeofanewFirmicutesspeciesbelongingtothedominanthumancolonicmicrobiota(‘Ruminococcusbicirculans’)revealstwochromosomesandaselectivecapacitytoutilizeplantglucans.
Wegmann,U.,Louis,P.,Goesmann,A.,Henrissat,B.,Duncan,S.H.&Flint,H.J.(2014).EnvironmentalMicroBIOLOGy,16(9),2879–2890.
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Therecentlyisolatedbacterialstrain80/3representsoneofthemostabundant16SrRNAphylotypesdetectedinthehealthyhumanlargeintestineandbelongstotheRuminococcaceaefamilyofFirmicutes.Thecompletedgenomesequencereportedhereisthefirstforamemberofthisimportantfamilyofbacteriafromthehumancolon.Thegenomecomprisestwolargechromosomesof2.24and0.73Mbp,leADIngustoproposethenameRuminococcusbicirculansforthisnewspecies.AnalysisofthecarbohydrateactiveenzymecomplementsuggestsanABIlitytoutilizecertainhemicelluloses,especiallyβ-glucansandxyloglucan,forgrowththatwasconfirmedexperimentally.Theenzymaticmachineryenablingthedegradationofcelluloseandxylanbyrelatedcellulolyticruminococciishoweverlackinginthisspecies.Whilethegenomeindicatedthecapacitytosynthesizepurines,pyrimidinesandall20aminoacids,onlygenesforthesynthesisofnicotinate,NAD+,NADP+andcoenzymeAweredetectedamongtheessentialvitaminsandco-factors,resultinginmultiplegrowthrequirements.Invivo,thesegrowthfactorsmustbesuppliedfromthediet,hostorothergutmicroorganisms.OtherfeaturesofecologicalinterestincludetwotypeIVpilins,multipleextracytoplasmicfunction-sigmafactors,aureaseandabilesalthydrolase.
CrystallizationandpreliminaryX-rayanalysisoftherhamnogalacturonanlyaseYesWfromBacillussubtilisstrain168,amemberofpolysaccharidelyasefamily11.
Ochiai,A.,Yamasaki,M.,Itoh,T.,Mikami,B.,Hashimoto,W.&Murata,K.(2006).ActaCrystallographicaSectionF:StructuralBiologyandCrystallizationCommunications,62(5),438-440.
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RhamnogalacturonanlyasesdegraderhamnogalacturonanI,amajorcomponentofpectin,throughaβ-eliminationreaction.YesWfromBacillussubtilisstrain168isanovelrhamnogalacturonanlyaseclassifiedintopolysaccharidelyasefamily11(PL-11).Theenzymewascrystallizedat293Kusingthesitting-dropvapour-diffusionmethodwith2-methyl-2,4-pentanediol(MPD)asaprecipitant.PreliminaryX-rayanalysisrevealedthattheYesWcrystalsbelongtospacegroupP21anddiffractto2.40Åresolution,withunit-cellparametersa=56.7,b=105.6,c=101.4Å,β=94.9°.ThisisthefirstreportonthecrystallizationandpreliminaryX-rayanalysisofafamilyPL-11rhamnogalacturonanlyase.
Degradationofdifferentpectinsbyfungi:correlationsandcontrastsbetweenthepectinolyticenzymesetsidentifiedingenomesandthegrowthonpectinsofdifferentorigin.
Benoit,I.,Coutinho,P.M.,Schols,H.A.,Gerlach,J.P.,Henrissat,B.&deVries,R.P.(2012).BMCGenomics,13(1),321.
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Background:Pectinsarediverseandverycomplexbiomoleculesandtheirstructuredependsontheplantspeciesandtissue.Itwaspreviouslyshownthatderivativesofpecticpolymersandoligosaccharidesfrompectinshavepositiveeffectsonhumanhealth.Toobtainspecificpecticoligosaccharides,highlydefinedenzymaticmixesarerequired.Filamentousfungiarespecializedinplantcellwalldegradationandsomeproduceabroadrangeofpectinases.Theymaythereforeshedlightontheenzymemixesneededforpartialhydrolysis.Results:Thegrowthprofilesof12fungionfourpectinsandfourstructuralelementsofpectinsshowthatthepresence/absenceofpectinolyticgenesinthefungalgenomeclearlycorrelateswiththeirabilitytodegradepectins.However,thiscorrelationislessclearwhenwezoomintothepecticstructuralelements.Conclusions:Thisstudyhighlightsthecomplexityofthemechanismsinvolvedinfungaldegradationofcomplexcarbonsourcessuchaspectins.Mininggenomesandcomparativegenomicsarepromisingfirststepstowardstheproductionofspecificpectinolyticfractions.
PlantcellwalldegradationbysaprophyticBacillussubtilisstrains:geneclustersresponsIBLeforrhamnogalacturonandepolymerization.
Ochiai,A.,Itoh,T.,Kawamata,A.,Hashimoto,W.&Murata,K.(2007).AppliedandEnvironmentalMicrobiology,73(12),3803-3813.
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PlantcellwalldegradationisapremiereventwhenBacillussubtilis,atypicalsaprophyticbacterium,invadesplants.HereweshowthedegradationsystemofrhamnogalacturonantypeI(RG-I),acomponentofpectinfromtheplantcellwall,inB.subtilisstrain168.Strain168cellsshowedasignificantgrowthonplantcellwallpolysaccharidessuchaspectin,polygalacturonan,andRG-Iasacarbonsource.DNAmicroarrayanalysisindicatedthatthreegeneclusters(yesOPQRSTUVWXYZ,ytePQRST,andybcMOPST-yBDABDE)areinduciblyexpressedinstrain168cellsgrownonRG-I.Cellsofanindustriallyimportantbacterium,B.subtilisstrainnatto,fermentingsoybeansalsoexpressthegeneclusterincludingtheyesseriesduringtheassimilationofsoybeanusedasacarbonsource.Amongproteinsencodedintheyescluster,YesWandYesXwerefoundtobenoveltypesofRGlyasesreleasingdisaccharidefromRG-I.GeneticandenzymaticpropertiesofYesWandYesXsuggestthatstrain168cellssecreteYesW,whichcatalyzestheinitialcleavageoftheRG-Imainchain,andtheresultantoligosaccharidesareconvertedtodisaccharidesthroughtheextracellularexotypeYesXreaction.ThedisaccharideisfinallydegradedintoitsconstituentmonosaccharidesthroughthereactionofintracellularunsaturatedgalacturonylhydrolasesYesRandYteR.ThisenzymaticrouteforRG-Idegradationinstrain168differssignificantlyfromthatinplant-pathogenicfungusAspergillusaculeatus.Thisis,toourknowledge,thefirstreportonthebacterialsystemforcompleteRG-Imainchaindegradation.
Generationofamonoclonalantibodyspecificto(1→5)-α-L-arabinan.
Willats,W.G.T.,Marcus,S.E.&Knox,J.P.(1998).CarbohydrateResearch,308(1),149-152.
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Aneoglycoprotein(aheptasaccharideof(1→5)-α-L-linked-arabinosylresidueslinkedtobovineserumalbumin)hasbeenusedtogeneratearatmonoclonalantibodyspecifictoalinearchainof(1→5)-α-L-arabinanwhichisastructuralfeatureofthesidechainsofpectins.Theantibody,designatedLM6,detected100ngofdebranchedsugarbeetarabinaninanimmunodotbindingassayand1µgofcommercialcitruspectininasimilarassay.Hapteninhibitionstudiesindicatedthattheantibodyrecognized5–6Araresiduesand50%inhibitionofantibodybindinginacompetitiveinhibitionELISAwasachievedwithca.2ng(21nM)of(1→5)-α-L-Arabinohexaose.Theantibodywillbeusefulforthelocalizationofarabinansinplanttissueandwillhaveusesintheanalysesofpectinstructure.Wereporthereonthelocalizationofthearabinanepitopeinlemonfruitsusingtissueprinting.
FunctionalidentificationoftwononredundantArabidopsisα(1,2)fucosyltransferasesspecifictoarabinogalactanproteins.
Wu,Y.,Williams,M.,Bernard,S.,Driouich,A.,Showalter,A.M.&Faik,A.(2010).JournalofBiologicalChemistry,285(18),13638-13645.
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Virtuallynothingisknownaboutthemechanismsandenzymesresponsiblefortheglycosylationofarabinogalactanproteins(AGPs).Theglycosyltransferase37familycontainsplant-specificenzymes,whichsuggestsinvolvementinplant-specificorganssuchasthecellwall.OurworkinghypothesisisthatAtFUT4andAtFUT6genesencodeα(1,2)fucosyltransferases(FUTs)forAGPs.Multiplelinesofevidencesupportthishypothesis.First,overexpressionofthetwogenesintobaccoBY2cells,knowntocontainnonfucosylatedAGPs,resultedinastainingoftransgeniccellswitheellectin,whichspecificallybindstoterminalα-linkedfucose.Second,monosaccharideanalysisbyhighpHanionexchangechromatographyandelectrosprayionizationmassspectrometryindicatedthepresenceoffucoseinAGPsfromtransgeniccelllinesbutnotinAGPsfromwildtypecells.Third,detergentextractsfrommicrosomalmembranespreparedfromtransgeniclineswereabletofucosylate,invitro,purifiedAGPsfromBY2wildtypecells.Susceptibilityof[14C]fucosylatedAGPstoα(1,2)fucosidase,andnottoα(1,3/4)fucosidase,indicatedthatanα(1,2)linkageisformed.FurThermore,dearabinosylatedAGPswerenotsubstrateacceptorsfortheseenzymes,indicatingthatarabinosylresiduesrepresentthefucosylationsitesonthesemolecules.Testingofseveralpolysaccharides,oligosaccharides,andglycoproteinsaspotentialsubstrateacceptorsinthefucosyltransferreactionsindicatedthatthetwoenzymesarespecificforAGPsbutarenotfunctionallyredundantbecausetheydifferentiallyfucosylatecertainAGPs.AtFUT4andAtFUT6arethefirstenzymestobecharacterizedforAGPglycosylationandfurtherourunderstandingofcellwallbiosynthesis.
Expression,purificationandcharacterizationofpectatelyaseAfromAspergillusnidulansinEscherichiacoli.
Zhao,Q.,Yuan,S.,Zhang,Y.,Zhu,H.,Dai,C.,Yang,F.&Han,F.(2007).WorldJournalofMicrobiologyandBiotechnology,23(8),1057-1064.
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PectatelyaseA(PelA)ofAspergillusnidulanswassuccessfullyexpressedinEscherichiacoliandeffectivelypurifiedusingaNi2+-nitrilotriacetate-agarosecolumn.EnzymeactivityoftherecombinantPelAcouldreach360Uml-1medium.TheexpressedPelAexhibiteditsoptimumlevelofactivityovertherangeofpH7.5–10at50°C.Mn2+,Ca2+,Fe2+,Mg2+andFe3+ionsstimulatedthepectatelyaseactivity,butCu2+andZn2+inhibitedit.TherecombinantPelAhadaVmaxof77µmolmin-1mg-1andanapparentKmof0.50mgml-1forpolygalacturonicacid.Low-esterifiedpectinwastheoptimumsubstrateforthePelA,whereashigher-esterifiedpectinwashardlycleavedbyit.PelAefficientlymaceratedmungbeanhypocotylsandpotatotubertissuesintosinglecells.
RestorationofmatureetiolatedcucumberhypocotylcellwallsusceptibilitytoexpansinbypretreatmentwithfungalpectinasesandEGTAinvitro.
Zhao,Q.,Yuan,S.,Wang,X.,Zhang,Y.,Zhu,H.&Lu,C.(2008).PlantPhysiology,147(4),1874-1885.
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Matureplantcellwallslosetheirabilitytoexpandandbecomeunresponsivetoexpansin.Thisphenomenonisbelievedtobeduetocross-linkingofhemicellulose,pectin,orphenolicgroupsinthewall.Byscreeningvarioushydrolyticenzymes,wefoundthatpretreatmentofnongrowing,heat-inactivated,basalcucumber(Cucumissativus)hypocotylswithpectinlyase(Pel1)fromAspergillusjaponicuscouldrestorereconstitutedexogenousexpansin-inducedextensioninmaturecellwallsinvitro.RecombinantpectatelyaseA(PelA)andpolygalacturonase(PG)fromAspergillusspp.exhibitedsimilarcapacitytoPel1.Pel1,PelA,andPGalsoenhancedthereconstitutedexpansin-inducedextensionoftheapical(elongating)segmentsofcucumberhypocotyls.However,theeffectiveconcentrationsofPelAandPGforenhancingthereconstitutedexpansin-inducedextensionweregreaterintheapicalsegmentsthaninthebasalsegments,whereasPel1behavedintheoppositemanner.Thesedataareconsistentwithdistributionofmoremethyl-esterifiedpectinincellwallsoftheapicalsegmentsandlessesterifiedpectininthebasalsegments.Associatedwiththedegreeofesterificationofpectin,morecalciumwasfoundincellwallsofbasalsegmentscomparedtoapicalsegments.PretreatmentofthecalciumchelatorEGTAcouldalsorestorematurecellwalls"susceptibilitytoexpansinbyremovingcalciumfrommaturecellwalls.Becauserecombinantpectinasesdonothydrolyzeotherwallpolysaccharides,andendoglucanase,xylanase,andproteasecannotrestorethematurewall"sextensibility,wecanconcludethatthepectinnetwork,especiallycalcium-pectatebridges,maybetheprimaryfactorthatdeterminescucumberhypocotylmaturecellwalls"unresponsivenesstoexpansin.
Tissue-specificrhamnogalacturonanIformsthegelwithhyperelasticproperties.
Mikshina,P.V.,Petrova,A.A.,Faizullin,D.A.,Zuev,Y.F.&Gorshkova,T.A.(2015).Biochemistry(Moscow),80(7),915-924.
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RhamnogalacturonansIarecomplexpectinpolysaccharidesextremelyvariableinstructureandpropertiesandwidelyrepresentedinvarioussources.ThecomplexityanddiversityofthestructureofrhamnogalacturonansIarethereasonsforthelimitedinformationaboutthepropertiesandsupramolecularorganizationofthesepolysaccharides,includingtherelationshipbetweentheseparametersandthefunctionsofrhamnogalacturonansIinplantcells.Inthepresentwork,ontheexampleofrhamnogalacturonanIfromflaxgelatinousfibers,theabilityofthistypeofpecticpolysaccharidestoformatphysiologicalconcentrationshydrogelswithhyperelasticpropertieswasrevealedforthefirsttime.AccordingtoIRspectroscopy,watermoleculesaremoretightlyretainedinthegellingrhamnogalacturonanIfromflaxfibercellwallincomparisonwiththenon-gellingrhamnogalacturonanIfromprimarycellwallofpotato.WithincreaseinstrengthofwaterbindingbyrhamnogalacturonanI,thereisanincreaseinelasticmodulusanddecreaseinPoisson’sratioofgelformedbythispolysaccharide.ThemodelofhyperelasticrhamnogalacturonanIcapturebylaterallyinteractingcellulosemicrofibrils,constructedusingthefiniteelementmethod,confirmedthesuitabilityofrhamnogalacturonanIgelwiththeestablishedpropertiesforthefunctioninthegelatinouscellwall,allowingconsiderationofthistissue-andstage-specificpecticpolysaccharideasanimportantfactorincreationofgelatinousfibercontractility.
PrioritizationofPolysaccharideUtilizationandControlofRegulatorActivationinBacteroidesthetaiotaomicron.
Schwalm,N.D.,Townsend,G.E.&Groisman,E.A.(2016).MolecularMicrobiology,104(1),32-45.
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Bacteroidesthetaiotaomicronisahumangutsymbioticbacteriumthatutilizesamyriadofhostdietaryandmucosalpolysaccharides.Theproteinsresponsiblefortheuptakeandbreakdownofmanyofthesepolysaccharidesaretranscriptionallyregulatedbyhybridtwo-componentsystems(HTCSs).Thesesystemsconsistofasinglepolypeptideharboringthedomainsofsensorkinasesandresponseregulators,andthus,arethoughttoautophosphorylateinresponsetospecificsignals.WenowreportthattheHTCSBT0366isphosphorylatedinvivowhenB.thetaiotaomicronexperiencestheBT0366inducerarabinanbutnotwhengrowninthepresenceofglucose.BT0366phosphorylationandtranscriptionofBT0366-activatedgenesrequirestheconservedpredictedsitesofphosphorylationinBT0366.Whenchondroitinsulfateisaddedtoarabinan-containingcultures,BT0366phosphorylationandtranscriptionofBT0366-activatedgenesareinhibitedandthebacteriumexhibitsdiauxicgrowth.Whereastwentyadditionalcombinationsofpolysaccharidesalsogiverisetodiauxicgrowth,othercombinationsresultinsynergisticorunalteredgrowthrelativetobacteriaexperiencingasinglepolysaccharide.ThedifferentstrategiesemployedbyB.thetaiotaomicronwhenfacedwithmultiplepolysaccharidesmayaiditscompetitivenessinthemammaliangut.
BiochemicalcharacterizationofrhamnosyltransferaseinvolvedinbiosynthesisofpecticrhamnogalacturonanIinplantcellwall.
Uehara,Y.,Tamura,S.,Maki,Y.,Yagyu,K.,Mizoguchi,T.,Tamiaki,H.,Imai,T.,Ishii,T.,Ohashi,T.,Fujiyama,K.&Ishimizu,T.(2017).BiochemicalandBiophysicalResearchCommunications,486(1),130-136.
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Thepectininplantcellwallsconsistsofthreedomains:homogalacturonan,rhamnogalacturonan(RG)-I,andRG-II.Itispredictedthataround50differentglycosyltransferasesarerequiredfortheirbiosynthesis.Amongthese,theactivitiesofonlyafewglycosyltransferaseshavebeendetectedbecausepecticoligosaccharidesarenotreadilyavailableforuseassubstrates.Inthisstudy,fluorogenicpyridylaminatedRG-I-backboneoligosaccharides(PA-RGs)with3–14degreesofpolymerization(DP)wereprepared.Usingtheseoligosaccharides,theactivityofRG-I:rhamnosyltransferase(RRT),involvedinthebiosynthesisoftheRG-Ibackbonediglycosylrepeatingunits(-4GalUAα1-2Rhaα1-),wasdetectedfromthemicrosomesofazukibeanepicotyls.RRTwasfoundtopreferlongeracceptorsubstrates,PA-RGswithaDP > 7,anditdoesnotrequireanymetalionsforitsactivity.RRTislocatedintheGolgiandendoplasmicreticulum.TheactivityofRRTcoincidedwithepicotylgrowth,suggestingthatRG-Ibiosynthesisisinvolvedinplantgrowth.
GelationofrhamnogalacturonanIisbasedongalactansidechaininteractionanddoesnotinvolvechemicalmodifications.
Mikshina,P.V.,Makshakova,O.N.,Petrova,A.A.,Gaifullina,I.Z.,Idiyatullin,B.Z.,Gorshkova,T.A.&Zuev,Y.F.(2017).CarbohydratePolymers,171,143-151.
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Thearticlepresentsthestructuralprinciplesofmicrowave-inducedformationofnewgeltypefrompecticrhamnogalacturonanI(RG-I).Thebackboneofgel-formingRG-Idoesnotcontainconsecutivegalacturonicresiduesandmodifyinggroupsthatcanbethecauseofjunctionzoneformationasitoccursincourseofclassicalwaysofpectingelation.MicrowaveirradiationdoesnotcausedestructionandchemicalmodificationsofRG-I.RemovalofhalfofgalactanchainsfromRG-Ileadstolossofgellingcapabilitypointingoutontheirleadingroleinthisprocess.RisingofintensityofthebandsattributedtogalactoseandglycosidiclinkagesinRG-Igelcomparingtosolutionwherethispolymerexistsasmoleculeassociateindicatesthatthespatialorganizationofgalactansingelischanged.AmodeloftheRG-Igelationisproposed:beingdestabilizedatvolumetricmicrowaveheatingRG-IassociatesarerepackedformingnetworkwhereRG-Imoleculesareentangledbygalactanchains.
ReciprocalPrioritizationtoDietaryGlycansbyGutBacteriainaCompetitiveEnvironmentPromotesStableCoexistence.
Tuncil,Y.E.,Xiao,Y.,Porter,N.T.,Reuhs,B.L.,Martens,E.C.&Hamaker,B.R.(2017).mBio,8(5),e01068-17.
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Whenpresentedwithnutrientmixtures,severalhumangutBacteroidesspeciesexhibithierarchicalutilizationofglycansthroughaphenomenonthatresemblescataboliterepression.However,itisunclearhowcloselytheseobservedphysiologicalchanges,oftenmeasuredbyalteredtranscriptionofglycanutilizationgenes,mirroractualglycandepletion.Tounderstandtheglycanprioritizationstrategiesoftwocloselyrelatedhumangutsymbionts,BacteroidesovatusandBacteroidesthetaiotaomicron,weperformedaseriesoftimecourseassaysinwhichbothspecieswereindividuallygrowninamediumwithsixdifferentglycansthatbothspeciescandegrade.Disappearanceofthesubstratesandtranscriptionofthecorrespondingpolysaccharideutilizationloci(PULs)weremeasured.Eachspeciesutilizedsomeglycansbeforeothers,butwithdifferentprioritiesperspecies,providinginsightintospecies-specifichierarchicalpreferences.Ingeneral,thepresenceofhighlyprioritizedglycansrepressedtranscriptionofgenesinvolvedinutilizinglower-prioritynutrients.However,transcriptionalsensitivitytosomeglycansvariedrelativetotheresidualconcentrationinthemedium,withsomePULsthattargethigh-prioritysubstratesremaininghighlyexpressedevenaftertheirtargetglycanhadbeenmostlydepleted.Coculturingoftheseorganismsinthesamemixtureshowedthatthehierarchicalordersgenerallyremainedthesame,promotingstablecoexistence.Polymerlengthwasfoundtobeacontributingfactorforglycanutilization,therebyaffectingitsplaceinthehierarchy.OurfindingsnotonlyelucidatehowB.ovatusandB.thetaiotaomicronstrategicallyaccessglycanstomaintaincoexistencebutalsosupporttheprioritizationofcarbohydrateutilizationbasedoncarbohydratestructure,advancingourunderstandingoftherelationshipsbetweendietandthegutmicrobiome.
品牌介绍
Megazyme品牌产品简介
来源：作者：人气：2149发表时间：2016-05-19 10:59:00【大 中 小】
Megazyme是一家全球性公司，专注于开发和提供用于饮料、谷物、乳制品、食品、饲料、发酵、生物燃料和葡萄酒产业用的分析试剂、酶和检测试剂盒。Megazyme的许多检测试剂盒产品已经为众多官方科学协会（包括AOAC, AACC , RACI, EBC和ICC等），经过严格的审核，批准认证为官方标准方法，确保以准确、可靠、定量和易于使用的测试方法，满足客户的质量诉求。
Megazyme的主要产品线包括：
◆ 检测试剂盒
◆ 酶
◆ 酶底物
◆ 碳水化合物
◆ 化学品/仪器
官网地址：http://www.megazyme.com
检测试剂盒特色产品：
货号
中文品名
用途
K-ACETAF
乙酸[AF法]检测试剂盒
酶法定量分析乙酸最广泛使用的方法
K-ACHDF
可吸收糖/膳食纤维检测试剂盒
酒精沉淀法测定膳食纤维
K-AMIAR
氨快速检测试剂盒
用于包括葡萄汁、葡萄酒以及其它食品饮料样品中氨含量的快速检测分析。
K-AMYL
直链淀粉/支链淀粉检测试剂盒
谷物淀粉和而粉中直链淀粉/支链淀粉比例和含量检测
K-ARAB
阿拉伯聚糖检测试剂盒
果汁浓缩液中阿拉伯聚糖的检测
K-ASNAM
L-天冬酰胺/L-谷氨酰胺和氨快速检测试剂盒
用于食品工业中丙烯酰胺前体、细胞培养基、以及上清液组分中、L-天冬酰胺，谷氨酰胺和氨的检测分析
K-ASPTM
阿斯巴甜检测试剂盒
专业用于测定饮料和食品中阿斯巴甜含量，操作简单
K-BETA3
β-淀粉酶检测试剂盒
适用于麦芽粉中β-淀粉酶的测定
K-BGLU
混合键β-葡聚糖检测试剂盒
测定谷物、荞麦粉、麦汁、啤酒及其它食品中混合键β-葡聚糖(1,3:1,4-β-D-葡聚糖)的含量
K-CERA
α-淀粉酶检测试剂盒
谷物和发酵液（真菌和细菌）中α-淀粉酶的分析测定
K-CITR
柠檬酸检测试剂盒
快速、可靠地检测食品、饮料和其它物料中柠檬酸（柠檬酸盐）含量
K-DLATE
乳酸快速检测试剂盒
快速、特异性检测饮料、肉类、奶制品和其它食品中L-乳酸和D-乳酸(乳酸盐）含量
K-EBHLG
酵母β-葡聚糖酶检测试剂盒
用于测量和分析酵母中1,3:1,6?-β-葡聚糖，也可以检测1,3-葡聚糖
K-ETSULPH
总亚硫酸检测试剂盒
测定葡萄酒、饮料、食品和其他物料中总亚硫酸含量（按二氧化硫计）的一种简单，高效，可靠的酶法检测方法
K-FRGLMQ
D-果糖/D-葡萄糖[MegaQuant法]检测试剂盒
适用于使用megaquant?色度计（505nm下）测定葡萄、葡萄汁和葡萄酒中D-果糖和D-葡萄糖的含量。
K-FRUC
果聚糖检测试剂盒
含有淀粉、蔗糖和其他糖类的植物提取物和食品中果聚糖的含量测定。
K-FRUGL
D-果糖/D-葡萄糖检测试剂盒
对植物和食品中果糖或葡萄糖含量的酶法紫外分光测定。
K-GALM
半乳甘露聚糖检测试剂盒
食品和植物产品中半乳甘露聚糖的含量检测
K-GLUC
D-葡萄糖[GOPOD]检测试剂盒
谷物提取物中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUHK
D-葡萄糖[HK]检测试剂盒
植物和食品中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUM
葡甘聚糖检测试剂盒
植物和食品中葡甘聚糖的含量测定。
K-INTDF
总膳食纤维检测试剂盒
总膳食纤维特定检测和分析
K-LACGAR
乳糖/D-半乳糖快速检测试剂盒
用于快速检测食品和植物产品中乳糖、D-半乳糖和L-阿拉伯糖
K-LACSU
乳糖/蔗糖/D-葡萄糖检测试剂盒
混合面粉和其它物料中蔗糖、乳糖和D-葡萄糖的测定
K-LACTUL
乳果糖检测试剂盒
特异性、快速和灵敏测量奶基样品中乳果糖含量
K-MANGL
D-甘露糖/D-果糖/D-葡萄糖检测试剂盒
适合测定植物产品和多糖酸性水解产物中D-甘露糖含量
K-MASUG
麦芽糖/蔗糖/D-葡萄糖检测试剂盒
在植物和食品中麦芽糖，蔗糖和葡萄糖的含量检测
K-PECID
胶质识别检测试剂盒
食品配料中果胶的鉴别
K-PHYT
植酸(总磷)检测试剂盒
食品和饲料样品植酸/总磷含量测量的简便方法。不需要通过阴离子交换色谱对植酸纯化，适合于大量样本分析
K-PYRUV
丙酮酸检测试剂盒
在啤酒、葡萄酒、果汁、食品和体液中丙酮酸分析
K-RAFGA
棉子糖/D-半乳糖检测试剂盒
快速测量植物材料和食品中棉子糖和半乳糖含量
K-RAFGL
棉子糖/蔗糖/D-半乳糖检测试剂盒
分析种子和种子粉中D-葡萄糖、蔗糖、棉子糖、水苏糖和毛蕊花糖含量。通过将棉子糖、水苏糖和毛蕊花糖酶解D-葡萄糖、D-果糖和半乳糖，从而测定葡萄糖含量来确定
K-SDAM
淀粉损伤检测试剂盒
谷物面粉中淀粉损伤的检测和分析
K-SUCGL
蔗糖/D-葡萄糖检测试剂盒
饮料、果汁、蜂蜜和食品中蔗糖和葡萄糖的分析
K-SUFRG
蔗糖/D-果糖/D-葡萄糖检测试剂盒
适用于植物和食品中蔗糖、D-葡萄糖和D-果糖的测定
K-TDFR
总膳食纤维检测试剂盒
总膳食纤维检测
K-TREH
海藻糖检测试剂盒
快速、可靠地检测食品、饮料和其它物料中海藻糖含量
K-URAMR
尿素/氨快速检测试剂盒
适用于水、饮料、乳制品和食品中尿素和氨的快速测定
K-URONIC
D-葡萄糖醛酸/D-半乳糖醛酸检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中六元糖醛酸含量（D-葡萄糖醛酸和D-半乳糖醛酸）
K-XYLOSE
D-木糖检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中D-木糖含量
K-YBGL
Beta葡聚糖[酵母和蘑菇]检测试剂盒
检测酵母和蘑菇制品中1,3:1,6-beta-葡聚糖和α-葡聚糖含量
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