Megazyme/Xylan (Beechwood; purified)/P-XYLNBE-10G/10 grams
商品编号：
P-XYLNBE-10G
品牌：
Megazyme INC
市场价：
¥2880.00
美元价：
1728.00
产品分类：
其他试剂
公司分类：
Other_reagents
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Highlypurifiedxylanfrombeechwoodforuseinresearch,biochemicalenzymeassaysandinvitrodiagnosticanalysis.
Suitableasareplacementforbirchwoodxylanasasubstrateforβ-xylanaseinDNSAreducingsugarassay.
Novelsubstratesfortheautomatedandmanualassayofendo-1,4-β-xylanase.
Mangan,D.,Cornaggia,C.,Liadova,A.,McCormack,N.,Ivory,R.,McKie,V.A.,Ormerod,A.&McCleary,D.V.(2017).CarbohydrateResearch,445,14-22.
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endo-1,4-β-Xylanase(EC3.2.1.8)isemployedacrossabroadrangeofindustriesincludinganimalfeed,brewing,baking,biofuels,detergentsandpulp(paper).Despiteitsimportance,arapid,reliable,reproducIBLe,automatableassayforthisenzymethatisbasedontheuseofachemicallydefinedsubstratehasnotbeendescribedtodate.Reportedhereinisanewenzymecoupledassayprocedure,termedtheXylX6assay,thatemploysanovelsubstrate,namely4,6-O-(3-ketobutylidene)-4-nitrophenyl-β-45-O-glucosyl-xylopentaoside.ThedevelopmentofthesubstrateandassociatedassayisdiscussedhereandtherelationshipbetweentheactivityvaluesobtainedwiththeXylX6assayversustrADItionalreducingsugarassaysanditsspecificityandreproducibilitywerethoroughlyinvestigated.
AComparisonofPolysaccharideSubstratesandReducingSugarMethodsfortheMeasurementofendo-1,4-β-Xylanase.
McCleary,B.V.&McGeough,P.(2015).Appl.Biochem.Biotechnol.,177(5),1152-1163.
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Themostcommonlyusedmethodforthemeasurementofthelevelofendo-xylanaseincommercialenzymepreparationsisthe3,5-dinitrosalicylicacid(DNS)reducingsugarmethodwithbirchwoodxylanassubstrate.ItiswellknownthatwiththeDNSmethod,muchhigherenzymeactivityvaluesareobtainedthanwiththeNelson-Somogyi(NS)reducingsugarmethod.Inthispaper,wehavecomparedtheDNSandNSreducingsugarassaysusingarangeofxylan-typesubstratesandaccuratelycomparedthemolarresponsefactorsforxyloseandarangeofxylo-oligosaccharides.PurifiedbeechwoodxylanorwheatarABInoxylanisshowntobeasuitablereplacementforbirchwoodxylanwhichisnolongercommerciallyavailable,anditisclearlydemonstratedthattheDNSmethodgrosslyoverestimatesendo-xylanaseactivity.UnliketheDNSassay,theNSassaygavetheequivalentcolourresponsewithequimolaramountsofxylose,xylobiose,xylotrioseandxylotetraosedemonstratingthatitaccuratelymeasuresthequantityofglycosidicbondscleavedbytheendo-xylanase.TheauthorsstronglyrecommendcessationoftheuseoftheDNSassayformeasurementofendo-xylanaseduetothefactthatthevaluesobtainedaregrosslyoverestimatedduetosecondaryreactionsincolourdevelopment.
PurificationandCharacterizationofaThermostableβ-mannanasefromBacillussubtilisBE-91:PotentialApplicationinInflammatoryDiseases.
Cheng,L.,Duan,S.,Feng,X.,Zheng,K.,Yang,Q.&Liu,Z.(2016).BioMedResearchInternational,ArticleID6380147.
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β-mannanasehasshowncompellingBIOLOGicalfunctionsbecauseofitsregulatoryrolesinmetabolism,inflammation,andoxidation.Thisstudyseparatedandpurifiedtheβ-mannanasefromBacillussubtilisBE-91,whichisapowerfulhemicellulose-degradingbacteriumusinga“two-step”methodcomprisingultrafiltrationandgelchromatography.Thepurifiedβ-mannanase(about28.2 kDa)showedhighspecificactivity(79,859.2 IU/mg).TheoptimumtemperatureandpHwere65°Cand6.0,respectively.Moreover,theenzymewashighlystableattemperaturesupto70°CandpH4.5-7.0.Theβ-mannanaseactivitywassignificantlyenhancedinthepresenceofMn+,Cu2+,Zn2+,Ca2+,Mg2+,andAl3+andstronglyinhibitedbyBa2+,andPb2+.KmandVmaxvaluesforlocustbeangumwere7.14 mg/mLand107.5 μmol/min/mLversus1.749 mg/mLand33.45 µmol/min/mLforKonjacglucomannan,respectively.Therefore,β-mannanasepurifiedbythisworkshowsstabilityathightemperaturesandinweaklyacidicorneutralenvironments.Basedonsuchdata,theβ-mannanasewillhavepotentialapplicationsasadietarysupplementintreatmentofinflammatoryprocesses.
Immobilizationandstabilizationofcommercialβ-1,4-endoxylanaseDepolTM333MDPbymultipointcovalentattachmentforxylanhydrolysis:Productionofprebiotics(xylo-oligosaccharides).
MartinsdeOliveira,S.,Moreno-Perez,S.,Romero-Fernández,M.,Fernandez-Lorente,G.,Rocha-Martin,J.&Guisan,J.M.(2017).BiocatalysisandBiotransformation,1-10.
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ThecommercialenzymeDepolTM333MDP(D333MDP)wasimmobilizedbymultipointcovalentattachmentonto10%cross-linkedagarosebeadssupporthighlyactivatedwithaldehydegroups.Theenzymeimmobilizationprocesswasveryefficient,retaining86%ofitsinitialcatalyticactivity.ThermalstabilityoftheimmobilizedD333MDPbiocatalystsvariedaccordingtotheincubationtimeoftheenzyme-support.Theoptimalimmobilizedbiocatalystwasproducedafter24 hofincubationunderalkalineconditionsandlongerincubationtimesresultedinalossofstability.Theoptimalimmobilizedbiocatalystwas60-and50-foldmorestableatpH5.5andpH7at50°Cthanthesolubleenzyme,respectively.ActivityandstabilityatpH5.5wereenhancedwhentheoptimalimmobilizedbiocatalystwasmodifiedbychemicalaminationoftheenzymesurface.Thechemicalaminationoftheimmobilizedenzymesurfacewas5-foldmorestableatpH5.5and50°Ccomparedwiththeunmodifiedimmobilizedbiocatalyst.Thebestimmobilizedbiocatalysts(containing100UI/gofsupport)wereevaluatedinthebeechwoodxylanhydrolysisreactionat50°CandpH5.5.80%ofthereducingsugarswerereleasedafter6 hofhydrolysiswiththeaminatedbiocatalyst.Xylanhydrolysisreactionwiththeaminatedbiocatalystwas80%fasterthanwiththenon-aminatedone.Thefinalcompositionofthexylooligosaccharides(XOS)obtainedwasidentifiedandquantifiedbyHPAEC-PADwhichshoweditwascomposedof90%ofxylobioseand5%ofxylotrioseandxylose.Theaminatedimmobilized-stabilizedbiocatalystwasusedforfourcyclesofhydrolysiswithnolossofcatalyticactivity,resultinginhighlyactiveandstablederivativesuitableforindustrialprocesses.
Improvementofthecatalyticcharacteristicsofasalt-tolerantGH10xylanasefromStreptomyceRocheiL10904.
Li,Q.,Sun,B.,Li,X.,Xiong,K.,Xu,Y.,Yang,R.,Hou,J.&Teng,C.(2017).InternationalJournalofBiologicalMacromolecules,InPress.
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AGH10xylanaseSrxyn10fromStreptomycerocheiL10904,anditstruncatedderivative,Srxyn10M,wereinvestigated.Bothdisplayedgreatsalt-tolerantability,retainingmorethan95%and91%activityafterincubationat37°Cfor1hin3.0Mand5.0MNaCl,respectively.Theyexhibitedaspecialhydrolyticpropertyofformingxylobioseasthemajorproductandproducedfewerxylosecompoundswhencombinedwithareportedxylanasewhiledigestingcorncobxylans.Themutant,Srxyn10M,wasconstructedfromSrxyn10bydeletingtheC-terminalcarbohydrate-bindingmodule.Itpossesseda3.26-foldhigherspecificactivityonbeechwoodxylanthanSrxyn10.Moreover,Srxyn10MshowedgreatersubstrateaffinityandcatalyticefficiencythanSrxyn10whenbeechwoodxylan,birchwoodxylan,andoat-speltxylanwereusedassubstrates.Thethermostabilitywasalsogreatlyimproved.Therefore,theapplicationpotentialwasmarkedlyenhancedbytheimprovementoftheseproperties.
Influenceofviscosityonthegrowthofhumangutmicrobiota.
Tamargo,A.,Cueva,C.,Álvarez,M.D.,Herranz,B.,Bartolomé,B.,Moreno-Arribas,M.V.&Laguna,L.(2017).FoodHydrocolloids,InPress.
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Numerousstudiessupportthebeneficialeffectsofdietaryfibre.Itiswellknownthatfibreincreasesviscosityatintestinallevel.Therefore,theeffectsoffibreongutmicrobiotacouldbeduenotonlybyitsintestinalbacteriafermentationbutalsototheincreaseinviscositybyitself.Theaimofthisstudywastoevaluatetheeffectofviscosityonthegrowthofgutmicrobiotaatphysiologicalconditions.Forthispurpose,fourcompartmentsfromagastrointestinalsimulator(simgi®)werefilledwithGutNutrientMedium(GNM)plusdifferentagarconcentrations(0,0.30,0.45and0.60%),inoculatedwithfaecalmicrobiota,andincubated48hunderanaerobicconditions.Sampleswerecollectedatthreetimepoints(0,24hand48h)forrepresentativeintestinalbacterialenumerationandrheologicalcharacterization.IncubationofGNMgelswithfaecalmicrobiotachangedthemediumviscosityovertime,evenwithconstantconditions(temperatureandpH).Insuchwaythat,inabsenceofagar(lowviscosity),viscosityslightlyincreasedovertime;however,inviscousmediums,viscositydecreasedovertime.Inrelationtothegrowthofgutmicrobiota,resultsshowedthatviscosityfavouredthegrowthoftotalanaerobesandClostridiumspp.;incontrast,totalnumberofaerobesandmembersofthegenusEnterococcuscorrelatednegativelywithviscosityincrement.Inconclusion,changesinintestinalviscosityseemtoselectivelymodifymicrobiotacomposition.Thisisapioneerworktounderstandtheeffectoffoodviscosityinthegastrointestinalsystem,showingthatviscosityisanimportantfactoritselftoconditionthegrowthofdifferentbacteria’sgroups.
DESCRIPTION
Xylan(Beechwood;purified)
CAS:9014-63-5
Synonyms:
4-O-methylglucuronoxylan
Stability:
>5yearsatroomtemperature
Properties:
Ash:3.2%
Moisture:1.7%
PhysicalDescription:Off-white,odourlesspowder
Applications:
Highlypurifiedxylanfrombeechwoodsuitableasareplacementforbirchwoodxylanasasubstrateforβ-xylanaseinDNSAreducingsugarassays.
品牌介绍
Megazyme品牌产品简介
来源：作者：人气：2149发表时间：2016-05-19 10:59:00【大 中 小】
Megazyme是一家全球性公司，专注于开发和提供用于饮料、谷物、乳制品、食品、饲料、发酵、生物燃料和葡萄酒产业用的分析试剂、酶和检测试剂盒。Megazyme的许多检测试剂盒产品已经为众多官方科学协会（包括AOAC, AACC , RACI, EBC和ICC等），经过严格的审核，批准认证为官方标准方法，确保以准确、可靠、定量和易于使用的测试方法，满足客户的质量诉求。
Megazyme的主要产品线包括：
◆ 检测试剂盒
◆ 酶
◆ 酶底物
◆ 碳水化合物
◆ 化学品/仪器
官网地址：http://www.megazyme.com
检测试剂盒特色产品：
货号
中文品名
用途
K-ACETAF
乙酸[AF法]检测试剂盒
酶法定量分析乙酸最广泛使用的方法
K-ACHDF
可吸收糖/膳食纤维检测试剂盒
酒精沉淀法测定膳食纤维
K-AMIAR
氨快速检测试剂盒
用于包括葡萄汁、葡萄酒以及其它食品饮料样品中氨含量的快速检测分析。
K-AMYL
直链淀粉/支链淀粉检测试剂盒
谷物淀粉和而粉中直链淀粉/支链淀粉比例和含量检测
K-ARAB
阿拉伯聚糖检测试剂盒
果汁浓缩液中阿拉伯聚糖的检测
K-ASNAM
L-天冬酰胺/L-谷氨酰胺和氨快速检测试剂盒
用于食品工业中丙烯酰胺前体、细胞培养基、以及上清液组分中、L-天冬酰胺，谷氨酰胺和氨的检测分析
K-ASPTM
阿斯巴甜检测试剂盒
专业用于测定饮料和食品中阿斯巴甜含量，操作简单
K-BETA3
β-淀粉酶检测试剂盒
适用于麦芽粉中β-淀粉酶的测定
K-BGLU
混合键β-葡聚糖检测试剂盒
测定谷物、荞麦粉、麦汁、啤酒及其它食品中混合键β-葡聚糖(1,3:1,4-β-D-葡聚糖)的含量
K-CERA
α-淀粉酶检测试剂盒
谷物和发酵液（真菌和细菌）中α-淀粉酶的分析测定
K-CITR
柠檬酸检测试剂盒
快速、可靠地检测食品、饮料和其它物料中柠檬酸（柠檬酸盐）含量
K-DLATE
乳酸快速检测试剂盒
快速、特异性检测饮料、肉类、奶制品和其它食品中L-乳酸和D-乳酸(乳酸盐）含量
K-EBHLG
酵母β-葡聚糖酶检测试剂盒
用于测量和分析酵母中1,3:1,6?-β-葡聚糖，也可以检测1,3-葡聚糖
K-ETSULPH
总亚硫酸检测试剂盒
测定葡萄酒、饮料、食品和其他物料中总亚硫酸含量（按二氧化硫计）的一种简单，高效，可靠的酶法检测方法
K-FRGLMQ
D-果糖/D-葡萄糖[MegaQuant法]检测试剂盒
适用于使用megaquant?色度计（505nm下）测定葡萄、葡萄汁和葡萄酒中D-果糖和D-葡萄糖的含量。
K-FRUC
果聚糖检测试剂盒
含有淀粉、蔗糖和其他糖类的植物提取物和食品中果聚糖的含量测定。
K-FRUGL
D-果糖/D-葡萄糖检测试剂盒
对植物和食品中果糖或葡萄糖含量的酶法紫外分光测定。
K-GALM
半乳甘露聚糖检测试剂盒
食品和植物产品中半乳甘露聚糖的含量检测
K-GLUC
D-葡萄糖[GOPOD]检测试剂盒
谷物提取物中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUHK
D-葡萄糖[HK]检测试剂盒
植物和食品中D-葡萄糖的含量测定，可以和其它Megazyme检测试剂盒联合使用。
K-GLUM
葡甘聚糖检测试剂盒
植物和食品中葡甘聚糖的含量测定。
K-INTDF
总膳食纤维检测试剂盒
总膳食纤维特定检测和分析
K-LACGAR
乳糖/D-半乳糖快速检测试剂盒
用于快速检测食品和植物产品中乳糖、D-半乳糖和L-阿拉伯糖
K-LACSU
乳糖/蔗糖/D-葡萄糖检测试剂盒
混合面粉和其它物料中蔗糖、乳糖和D-葡萄糖的测定
K-LACTUL
乳果糖检测试剂盒
特异性、快速和灵敏测量奶基样品中乳果糖含量
K-MANGL
D-甘露糖/D-果糖/D-葡萄糖检测试剂盒
适合测定植物产品和多糖酸性水解产物中D-甘露糖含量
K-MASUG
麦芽糖/蔗糖/D-葡萄糖检测试剂盒
在植物和食品中麦芽糖，蔗糖和葡萄糖的含量检测
K-PECID
胶质识别检测试剂盒
食品配料中果胶的鉴别
K-PHYT
植酸(总磷)检测试剂盒
食品和饲料样品植酸/总磷含量测量的简便方法。不需要通过阴离子交换色谱对植酸纯化，适合于大量样本分析
K-PYRUV
丙酮酸检测试剂盒
在啤酒、葡萄酒、果汁、食品和体液中丙酮酸分析
K-RAFGA
棉子糖/D-半乳糖检测试剂盒
快速测量植物材料和食品中棉子糖和半乳糖含量
K-RAFGL
棉子糖/蔗糖/D-半乳糖检测试剂盒
分析种子和种子粉中D-葡萄糖、蔗糖、棉子糖、水苏糖和毛蕊花糖含量。通过将棉子糖、水苏糖和毛蕊花糖酶解D-葡萄糖、D-果糖和半乳糖，从而测定葡萄糖含量来确定
K-SDAM
淀粉损伤检测试剂盒
谷物面粉中淀粉损伤的检测和分析
K-SUCGL
蔗糖/D-葡萄糖检测试剂盒
饮料、果汁、蜂蜜和食品中蔗糖和葡萄糖的分析
K-SUFRG
蔗糖/D-果糖/D-葡萄糖检测试剂盒
适用于植物和食品中蔗糖、D-葡萄糖和D-果糖的测定
K-TDFR
总膳食纤维检测试剂盒
总膳食纤维检测
K-TREH
海藻糖检测试剂盒
快速、可靠地检测食品、饮料和其它物料中海藻糖含量
K-URAMR
尿素/氨快速检测试剂盒
适用于水、饮料、乳制品和食品中尿素和氨的快速测定
K-URONIC
D-葡萄糖醛酸/D-半乳糖醛酸检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中六元糖醛酸含量（D-葡萄糖醛酸和D-半乳糖醛酸）
K-XYLOSE
D-木糖检测试剂盒
简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中D-木糖含量
K-YBGL
Beta葡聚糖[酵母和蘑菇]检测试剂盒
检测酵母和蘑菇制品中1,3:1,6-beta-葡聚糖和α-葡聚糖含量
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